• The Korea Journal of Herbology
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• v.32 no.6
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• pp.9-15
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• 2017

Objectives : Traditional medicines isolated from natural products often have positive effects in the prevention and healing of various immune disorders, such as allergy and atopic inflammation. Scrophulariae Radix (SR) been used in oriental medicine used for treatment of acute and chronic inflammatory diseases. Mast cells are known to play important roles in the initiation of allergic reactions. In this study, we investigated the effects of SR ethanol extract on inflammatory responses in IgE-stimulated RBL-2H3 mast cells. Methods : Rat basophilic leukemia RBL-2H3 cells were purchased from Korean Cell Line Bank (KCLB No. 22256). Cell viability was measured by MTT assay. Assays for ${\beta}-Hexosaminidase$ Secretion : RBL-2H3 cells were sensitized with dinitrophenyl-ImmunoglobulinE (DNP IgE). The next antigen DNP-BSA ($25ng/m{\ell}$) was added for 10 minutes and the reaction was terminated after 5 minutes in the ice bath. To determine ${\beta}-Hexosaminidase$ release, supernatants were aliquoted into 96-well plates. Samples were mixed with substrate solution and incubated for 1 h at $37^{\circ}C$. Absorbance was measured with a spectrophotometer at 405 nm. IL-4 and tumor necrosis $factor-{\alpha}$($TNF-{\alpha}$) concentrations in cell culture supernatants were measured using enzyme-linked immunosorbent assay (ELISA) kits. Results : The cytotoxicity of SRE in RBL-2H3 cells was less than 5%. SRE inhibited DNP-IgE-imduced degranulation of mast cells in RBL-2H3 cells. Also significantly decreased the levels of inflammatory cytokine, IL-4 and TNF-alpha. In this study, the SRE showed potential anti-allergic and antiinflammatory. Conclusions : These results indicate that SRE could be inhibit the allergic response through suppressing the mast cell activation.

• Animal Bioscience
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• v.35 no.6
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• pp.892-901
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• 2022

Objective: This study was performed to investigate the potential effect of wheat phytase on long-chain inorganic polyphosphate (polyP)-mediated interleukin 8 (IL-8) signaling in an intestinal epithelial cell line, HT-29 cells. Methods: Cell viability and the release of the pro-inflammatory cytokine IL-8 in HT-29 cells exposed to polyP1150 (average of 1,150 phosphate residues) treated with or without wheat phytase were measured by the EZ-CYTOX kit and the IL-8 ELISA kit, respectively. Also, the activation of cellular inflammatory factors NF-κB and MAPK (p38 and ERK 1/2) in HT-29 cells was investigated using ELISA kits. Results: PolyP1150 negatively affected the viability of HT-29 cells in a dose-dependent manner. However, 100 mM polyP1150 dephosphorylated by wheat phytase increased cell viability by 1.4-fold over that of the intact substrate. Moreover, the 24 h exposure of cells to enzyme-treated 50 mM polyP1150 reduced the secretion of IL-8 and the activation of NF-κB by 9% and 19%, respectively, compared to the intact substrate. PolyP1150 (25 and 50 mM) dephosphorylated by the enzyme induced the activation of p38 MAPK via phosphorylation to 2.3 and 1.4-fold, respectively, compared to intact substrate, even though it had little effect on the expression of ERK 1/2 via phosphorylation. Conclusion: Wheat phytase could attenuate polyP1150-induced IL-8 release in HT-29 cells through NF-κB, independent of MAP kinases p38 and ERK. Thus, wheat phytase may alleviate inflammatory responses including hypercytokinemia caused by bacterial polyP infection in animals. Therefore, wheat phytase has the potential as an anti-inflammatory therapeutic supplement in animal husbandry.

• Transactions of the Korean hydrogen and new energy society
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• v.33 no.4
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• pp.284-292
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• 2022

Hydrogen is promising a candidate for energy supporting the carbon neutrality policy for greenhouse gas reduction, which is being promoted in several countries, including Korea. Although challenging efforts-such as lowering the costs of green hydrogen production and fuel cells-remain, hydrogen fuel cell electric vehicles (FCEVs) are expected to play a significant role in the energy transition from fossil fuels to renewable energy. In line with this objective, the hydrogen FCEV working group in the International Organization for Standardization (ISO) compiled and revised international standards related to hydrogen refueling stations as of 2019. A well-established hydrogen quality management system based on the standard documents will increase the reliability of hydrogen charging stations and accelerate the use of FCEVs. In this study, among the published ISO standards and other references, the main requirements for managing charging stations and developing related techniques were summarized and explained. To respond preemptively to the growing FCEV market, a continuous hydrogen quality monitoring method suitable for use at hydrogen charging stations was proposed.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.58 no.3
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• pp.230-240
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• 2022

In general, a high tension on the anchor and chain is placed when a ship at anchor is subjected to heavy weather. Mariners have to pay attention to whether dragging anchor occurs to keep the safety of the ship at anchorage since it is difficult to maintain the stable motion of ship and it causes collisions with other ships nearby. In this paper, the ship motion against the external forces was shown to obtain the fundamental data about characteristic of holding power due to nature of seabed at anchor, so practical trials were carried out in rocky area and muddy area using a trial ship around coastal area of South Korea. In muddy seabed, holding power showed reasonable tension values depending on the distance from anchor position of continuing swing motions of a ship corresponding to wind force. Meanwhile in rocky seabed, tension values on the chain appeared very high occasionally regardless of the distance from the anchor position and seemed to exceed its holding power to be the breaking strain of the chain although weather was not in a severe condition. Therefore, some of the cables laid on the seabed were presumed to be caught in a crack on the rock. It is assumed that even a small amount of external force may cause the chain to break in a moment in rocky seabed. Additionally, wind and current forces had a somewhat contradictory effect on holding power of the ship between them.

• The Journal of the Korea institute of electronic communication sciences
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• v.17 no.4
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• pp.577-586
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• 2022

Detection and classification of steel surface defects are critical for product quality control in the steel industry. However, due to its low accuracy and slow speed, the traditional approach cannot be effectively used in a production line. The current, widely used algorithm (based on deep learning) has an accuracy problem, and there are still rooms for development. This paper proposes a method of steel surface defect detection combining EfficientNetV2 for image classification and YOLOv5 as an object detector. Shorter training time and high accuracy are advantages of this model. Firstly, the image input into EfficientNetV2 model classifies defect classes and predicts probability of having defects. If the probability of having a defect is less than 0.25, the algorithm directly recognizes that the sample has no defects. Otherwise, the samples are further input into YOLOv5 to accomplish the defect detection process on the metal surface. Experiments show that proposed model has good performance on the NEU dataset with an accuracy of 98.3%. Simultaneously, the average training speed is shorter than other models.

• Animal Bioscience
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• v.36 no.3
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• pp.492-497
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• 2023

Objective: The objectives were to demonstrate that the nitrogen and energy in pig urine supplemented with hydrochloric acid (HCl) are not volatilized and to determine the minimum amount of HCl required for nitrogen preservation from pig urine. Methods: In Exp. 1, urine samples of 3.0 L each with 5 different nitrogen concentrations were divided into 2 groups: 1.5 L of urine added with i) 100 mL of distilled water or ii) 100 mL of 6 N HCl. The urine in open plastic containers was placed on a laboratory table at room temperature for 10 d. The weight, nitrogen concentration, and gross energy concentration of the urine samples were determined every 2 d. In Exp. 2, three urine samples with different nitrogen concentrations were added with different amounts of 6 N HCl to obtain varying pH values. All urine samples were placed on a laboratory table for 5 d followed by nitrogen analysis. Results: Nitrogen amounts in urine supplemented with distilled water decreased linearly with time, whereas those supplemented with 6 N HCl remained constant. Based on the linear broken-line analysis, nitrogen was not volatilized at a pH below 5.12 (standard error = 0.71 and p<0.01). In Exp. 3, an equation for determining the amount of 6 N HCl to preserve nitrogen in pig urine was developed: additional 6 N HCl (mL) to 100 mL of urine = 3.83×nitrogen in urine (g/100 mL)+0.71 with R² = 0.96 and p<0.01. If 62.7 g/d of nitrogen is excreted, at least 240 mL of 6 N HCl should be added to the urine collection container. Conclusion: Nitrogen in pig urine is not volatilized at a pH below 5.12 at room temperature and the amount of 6 N HCl required for nitrogen preservation may be up to 240 mL per day for a 110-kg pig depending on urinary nitrogen excretion.

• Korean Journal of Agricultural Science
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• v.49 no.1
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• pp.19-29
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• 2022

The present experiment was conducted to evaluate the effects of single-sex or mixed rearing on the growth and laying performance, blood parameters, egg quality, and feather scores of laying hens in an aviary system. A total of 2,928 (females, 2,856; males, 72), Hy-Line Brown chickens at 20 weeks were evenly assigned to two treatments: termed here the single-sex (only female) and mixed-sex (female to male 20 : 1) conditions. They were allowed an ad libitum diet for 20 weeks in an aviary system. There were eight replicates of each treatment, each consisting of 12.42 m²·birds^-1 (n=183), respectively. The body weight and uniformity in the mixed-sex condition were higher than those in the single-sex condition, but egg production did not show any significant differences between the two types of treatment for 20 weeks. The mixed-sex group showed an effect through stress parameters (corticosterone), whereas the feather score on the back was low at the end of the experiment. These results suggest that the presence of males affect corticosterone level for mating but can reduce the vigilance and aggressive behavior of laying hens.

• Transactions of Materials Processing
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• v.32 no.3
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• pp.153-159
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• 2023

This paper presents the optimal design of a heating system using radiant heating elements for application in smart farms. Smart farming, an advanced agricultural technology, is based on artificial intelligence and the internet of things and promotes crop production. Temperature and humidity regulation is critical in smart farms, and thus, a heating system is essential. Radiant heating elements are devices that generate heat using electrical energy. Among other applications, radiant heating elements are used for environmental control and heating in smart farm greenhouses. The performance of these elements is directly related to their electrical energy consumption. Therefore, achieving a balance between efficient electrical energy consumption and maximum heating performance in smart farms is crucial for the optimal design of radiant heating elements. In this study, the size, electrical energy supply, heat generation efficiency, and heating performance of radiant heating elements used in these heating systems were investigated. The effects of the size and electrical energy supply of radiant heating elements on the heating performance were experimentally analyzed. As the radiant heating element size increased, the heat generation efficiency improved, but the electrical energy consumption also increased. In addition, increasing the electrical energy supply improved both the heat generation efficiency and heating performance of the radiant heating elements. Based on these results, a method for determining the optimal size and electrical energy supply of radiant heating elements was proposed, and it reduced the electrical energy consumption while maintaining an appropriate heating performance in smart farms. These research findings are expected to contribute to energy conservation and performance improvement in smart farming.

• The Research Journal of the Costume Culture
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• v.31 no.4
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• pp.411-429
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• 2023

Modernism is an internationally accepted design style. In addition, traditional Korean costume is not the clothing of the past, but its form is also used in modern fashion. Therefore, the purpose of this study is to examine the social and cultural contents of the morphological structure of traditional Korean costume, in order to derive the design characteristics of modernism and examine the global applicability of traditional Korean costume structure. For this study, photographic materials from museums, schematic drawings of relics, and Hanbok production books published by government agencies were analyzed. Further, this study examined traditional Korean costume, especially the modernism characteristics derived from Paul Greenhalgh and previous studies. The results are as follows. First, The traditional Korean costume is a cut based on basic shapes, along with a silhouette appears according to the background of the times. Second, the characteristics of modernism can be divided into universality, functionality, and simplicity. Third, as a result of analyzing the form structure of traditional Korean costume based on the characteristics of modernism, universality is expressed as standardized form, functionality as practical aesthetics, and simplicity as sustainable diversity. Furthermore, Korean costume has a difference in that was greatly influenced by various Eastern ideas and aesthetics of the late Joseon Dynasty. These findings provide a perspective of modern reinterpretation of the uniqueness and universality of traditional Korean costume in line with globalization by utilizing the design characteristics of modernism, an international style.

• The Korea Journal of Herbology
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• v.24 no.1
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• pp.169-178
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• 2009

Objectives : In this study, the effects of ethylacetate extract of Ostericum koreanum on inflammation in RAW264.7 cells were investigated. Methods : Dried roots of Ostericum koreanum was extracted with 80% methanol for 24 h, and then fractionated with n-butanol, n-hexan and ethylacetate. RAW264.7 cells, a mouse macrophage line were incubated with different concentrations of the extract for 30 min and then stimulated with LPS at indicated times. Cell toxicity was determined by MTT assay. The concentrations of nitric oxide (NO) and prostaglandin E2 ($PGE_2$) were measured by Griess assay and enzyme immunoassay (EIA), respectively. The expressions of inducible nitric oxide synthease (iNOS) and cyclooxyganase (COX) -2 mRNA and protein were determined by RT-PCR and Western blot. Results : The methanol extract of Ostericum koreanuman and its fractions were significantly inhibited the NO and PGE2 productions in LPS-stimulated RAW264.7 cells. Among the fractions of Ostericum koreanuman the ethylacetate fraction was more strongly inhibited NO and $PGE_2$ productions compared with other fractions. The ethylacetate fraction was also suppressed LPS-induced mRNA expressions of iNOS and its protein levels in RAW264.7 cells. Conclusions : This study suggests that the ethylacetate fraction of Ostericum koreanum may have an anti-inflammatory property through suppressing inflammatory mediator productions in activated macrophages, suggesting have a therapeutic potential for the treatment of various inflammatory diseases.