• Title/Summary/Keyword: Product Recovery

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Characterization of Streptomyces sp. KSM-35 and Purificaton of Its Maltotetraose Forming Amylase (Streptomyces sp. KSM-35의 특성과 Maltotetraose 생산성 아밀라제의 정제)

  • Cha, Jin;Kim, Young-Bae;Seo, Byung-Cheol;Park, Kwan-Wha
    • Korean Journal of Food Science and Technology
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    • v.26 no.5
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    • pp.633-637
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    • 1994
  • A bacterial strain KSM-35 producing maltotetraose forming amylase was isolated from compost and identified as Streptomyces based on its morphological, cultural, and physiological characteristics. The amylase from Streptomyces sp. KSM-35 culture filtrate was purified by ammonium sulfate precipitation, followed by the liquid chromatographic procedures using DEAE-Toyo pearl and sephadex G-100 with 27.1% activity recovery. The molecular weight of the enzyme was estimated to be 50,000 and the isoelectric point 4.3. The main product by the amylase from soluble starch was maltotetraose which accounted for 56% of all the oligosaccharides detected after 26 hrs of reaction. Maltose (20%o) and maltotriose (16%) were the next important byproducts while glucose and maltopentaose were detected as traces.

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Empirical Analysis on the Factors Affecting the Consumption of Aquatic Products in China Using Panel Data (패널자료를 이용한 중국 수산물소비지출에 영향을 미치는 요인에 관한 연구)

  • Lin, Xuemei;Kim, Ki-Soo
    • The Journal of Fisheries Business Administration
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    • v.44 no.2
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    • pp.19-33
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    • 2013
  • China is the world's largest producer of aquatic products and its fishery sector is comprised primarily of aquaculture facilities, both fresh and seawater, and a much smaller wild catch component. The expansion of the aquaculture sector continues to boost China's aquatic output. The increase is attributable to several factors. Among them rapidly growing domestic demand is no doubt one of the most important reasons. Rapid economic growth and rising disposable income are influencing domestic consumption and the economic recovery of major import markets is further stimulating consumption of China's aquatic products. This paper tries to examine the status, trend and reasons of consumption of aquatic products in China. Based on the analysis on the consumption of aquatic products and its change, this paper utilizes panel regression model to estimate the affecting factors of aquatic products consumption in China. By using panel data between 1997 and 2010, the results indicate that income, urbanization, aging and dummy variable have positive effects and fish price index has negative effect on China's aquatic products consumption as predicted. However, effects of meat consumption and education on fishery consumption are totally opposite with our predictions. In other words, it turns out that meat consumption has positive effect on fish consumption while education turns out to have negative effect. Finally the member of household does not show any significant effect on consumption of aquatic products in China.

A New Complex-Number Multiplication Algorithm using Radix-4 Booth Recoding and RB Arithmetic, and a 10-bit CMAC Core Design (Radix-4 Booth Recoding과 RB 연산을 이용한 새로운 복소수 승산 알고리듬 및 10-bit CMAC코어 설계)

  • 김호하;신경욱
    • Journal of the Korean Institute of Telematics and Electronics C
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    • v.35C no.9
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    • pp.11-20
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    • 1998
  • High-speed complex-number arithmetic units are essential to baseband signal processing of modern digital communication systems such as channel equalization, timing recovery, modulation and demodulation. In this paper, a new complex-number multiplication algorithm is proposed, which is based on redundant binary (RB) arithmetic combined with radix-4 Booth recoding scheme. The proposed algorithm reduces the number of partial product by one-half as compared with the conventional direct method using real-number multipliers and adders. It also leads to a highly parallel architecture and simplified circuit, resulting in high-speed operation and low power dissipation. To demonstrate the proposed algorithm, a prototype complex-number multiplier-accumulator (CMAC) core with 10-bit operands has been designed using 0.8-$\mu\textrm{m}$ N-Well CMOS technology. The designed CMAC core contains about 18,000 transistors on the area of about 1.60 ${\times}$ 1.93 $\textrm{mm}^2$. The functional and speed test results show that it can operate with 120-MHz clock at V$\sub$DD/=3.3-V, and its power consumption is given to about 63-mW.

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Study on Oil Production from Pyrolysis of Mixed Plastic Waste Using Multidimensional Chromatography (Multidimensional Chromatography/Mass Spectrometry를 이용한 혼합 폐플라스틱의 열분해 오일 특성 평가에 관한 연구)

  • 김석완
    • Journal of Environmental Science International
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    • v.11 no.4
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    • pp.375-382
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    • 2002
  • The total hydrocarbon distribution of oil products obtained from the pyrolysis of four kinds of mixtures of polyethylene-polystyrene waste has been studied by multidimensional chromatography(high performance liquid chromatography followed by capillary gas chromatography)/mass spectrometry. Saturated, unsaturated and aromatic hydrocarbons in oil products were selectively pre-separated according to structural groups by HPLC and the weight fraction of each group was estimated by analysis of each component using GC-FID response factors. The hydrocarbon distribution of aliphatic fraction consists of $C_{5}$ to $C_{25}$ saturated and unsaturated hydrocarbons. And that of aromatics fraction consists of benzene, toluene, xylene, styrene, propenyl benzene, naphthalene, and some of derivatives. Pyrolysis temperature did not affect the ratio of total weight fraction of aliphatic over aromatic hydrocarbon distribution in case of PS only and PE-PS mixtures (1:1 and 1:4 wt. ratio) as a feed while affected the ratio of total wt. fraction in case of PE only. The optimal temperature for the maximum oil production was $600^{\circ}C$ for pyrolysis of PS and 1:1 and 1:4 mixtures of PE and PS. The optimal condition for aromatic recovery was $600^{\circ}C$ with 1:1 mixture of PE and PS. In this condition, aromatic was produced up to 90% of total oil product. The maximum yield of toluene, xylene, styrene, and propenyl benzene were 8.6, 8.9, 51.0 and 7.4% of feed for pyrolysis PS at $700^{\circ}C$, respectively. However, only 1.3% naphthalene was recovered at $700^{\circ}C$ with 1:1 PE:PS(by wt.).

Studies on Whole Cell Immobilized Glucose Isomerase - I. Preparation and Properties of Whole Cell Immobilized Glucose Isomerase - (포도당 이성화 효소의 세포 고정화에 관한 연구 - I. 세포 고정화 효소의 제조와 성질 -)

  • Ahn, Byung-Yoon;Byun, Si-Myung
    • Korean Journal of Food Science and Technology
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    • v.11 no.3
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    • pp.192-199
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    • 1979
  • With cells of Streptomyces spp K-45 isolated from soil, the immobilization of glucose isomerase by a series of treatments ; heat, carefully manipulated drying, extrusion with a thickening agent, and glutaraldehyde-induced crosslinking, was presented. This was aimed to obtain a mechanically stable form of whole cell containing glucose isomerase. The resulted pellet form had a good mechanical strength, compared with a commercial product, and showed 26 % of the activity recovery. The specific activity was 48.1 units per g of the dry material. The immobilized glucose isomerase generally showed properties similar to those of the soluble enzyme ; optimal pH at $7.5{\sim}9.0$, optimal temperature at $80{\sim}85^{\circ}C$, activation energy of 10.9 kcal/mole, and $K_m$ for glucose of 10.9M. The immobilized enzyme was very thermostable and pH stable.

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Radioprotective Potential of Panax ginseng: Current Status and Future Prospectives (고려인삼의 방사선 방어효과에 대한 연구현황과 전망)

  • Nam, Ki-Yeul;Park, Jong-Dae;Choi, Jae-Eul
    • Korean Journal of Medicinal Crop Science
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    • v.19 no.4
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    • pp.287-299
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    • 2011
  • Pharmacological effects of Panax ginseng have been demonstrated in cardiovascular system, endocrine secretion and immune system, together with antitumor, anti-stress and anti-oxidant activities. Modern scientific data show protective effect of ginseng against bone marrow cell death, increased survival rate of experimental animals, recovery of hematopoietic injury, immunopotentiation, reduction of damaged intestinal epithelial cells, inhibition of mutagenesis and effective protection against testicular damages, caused by radiation exposure. And also, ginseng acts in indirect fashion to protect radical processes by inhibition of initiation of free radical processes and thus reduces the radiation damages. The research has made much progress, but still insufficient to fully uncover the action mechanism of ginseng components on the molecule level. This review provides the usefulness of natural product, showing no toxic effects, as an radioprotective agent. Furthermore, the further clinical trials on radioprotection of ginseng need to be highly done to clarify its scientific application. The effective components of ginseng has been known as ginsenosides. Considering that each of these ginsenosides has pharmacological effect, it seems likely that non-saponin components might have radioprotective effects superior to those of ginsenosides, suggesting its active ingredients to be non-saponin series. These results also show that the combined effects of saponin and non-saponin components play an important role in the radioprotective effects of ginseng.

A Study on the Selective Leaching of the Copper Component by Sulfation Process (황산화 배소법에 의한 구리성분의 선택적 침출연구)

  • Kim, Woo Jin;Kim, Joon Soo;Kim, Myong Jun;Tran, Tam;Lee, Jin-Young;Shin, Shun-Myung
    • Resources Recycling
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    • v.25 no.5
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    • pp.57-63
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    • 2016
  • This study were carried out sulfation roasting and selective leaching test for the effective recovery of copper component in concentrate obtained by froth floatation of Autrallian low grade copper ore. The optimum conditions of sulfation roasting were temp. $450^{\circ}C$, $Na_2SO_4$ 2 mole ratio and time 1.5 h, and then selective leaching were room temperature and $H_2O$ or 1M $H_2SO_4$ solutions. Leaching efficiency of optimum sulfation product were 90 wt.% of copper, 20 wt.% of iron and 15wt.% of nickel elements. In this results, it was possible to selective decomposition leaching of the copper component under optimum conditions in this research.

Phenanthrene Derivatives, 3,5-Dimethoxyphenanthrene-2,7-diol and Batatasin-I, as Non-Polar Standard Marker Compounds for Dioscorea Rhizoma

  • Yoon, Kee-Dong;Yang, Min-Hye;Nam, Sang-Il;Park, Ju-Hyun;Kim, Young-Choong;Kim, Jin-Woong
    • Natural Product Sciences
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    • v.13 no.4
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    • pp.378-383
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    • 2007
  • Phenathrene derivatives, such as batatasins, are well-known constituents in Dioscorea Rhizoma. Although phenanthrenes have been reported as representative compounds in this plant, standard markers for quality control have been focused on the polar constituents (saponins and purine derivatives). Herein, simple, rapid and reliable HPLC method was developed to determine 3,5-dimethoxyphenanthrene-2,7-diol (DMP) and batatasin-I (BA-I) as non-polar standard maker compounds of Dioscorea Rhizoma. DMP and BA-I were analyzed under optimized HPLC conditions [column: Columbus $5{\mu}$ C18 100A ($30{\times}4.6mm$ i.d., $5{\mu}m$; mobile phase: $H_2O$ with 0.025% $CH_3COOH$ (v/v) for solvent A and $CH_3CN$ with 0.025% $CH_3COOH$ (v/v) for solvent B, gradient elution; flow rate: 2 mL/min; detection: 260 nm), and each experiment was finished within 13 min. Good linearity was achieved in the range from 0.5 to $10.0{\mu}g/mL$ for each compound, and intra- and inter-day precision were in the acceptable levels. The recovery test were performed with three different Dioscorea Rhizoma samples (D. opposita, D. batatas and D. japonica), and showed its accuracy values in the range of 97.2 - 102.8% for three different concentrations of DMP and BA-I. The content levels of DMP and BA-I were ranged under 0.0020%. These results demonstrated that amounts of DMP and BA-I are easily determined with conventional HPLC-UV-DAD method although the content levels were lower than those of saponins and allantoin in Dioscorea Rhizoma. This HPLC method could be used for quality control of various Dioscorea preparations.

Simultaneous Determination of Paeoniflorin, Trans-cinnamic Acid, Schisandrin and Glycyrrhizin in So-Cheong-Ryong-Tang by HPLC-DAD and HPLC-ESI-MS

  • Lee, Mi-Kyeong;Lee, Ki-Yong;Park, Jung-Hyun;Kim, Seung-Hyun;Choi, Ok-Gyung;Park, Jin-Ho;Cho, Jung-Hee;Kim, Do-Hoon;Baek, Ju-Hyun;Oh, Mi-Hyune;Kim, Hyo-Jin;Sung, Sang-Hyun
    • Natural Product Sciences
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    • v.16 no.1
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    • pp.26-31
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    • 2010
  • High performance liquid chromatographic method with diode-array detection (HPLC-DAD) has been performed for the simultaneous determination of four marker constituents, paeoniflorin, trans-cinnamic acid, schisandrin and glycyrrhizin in traditional herbal medicinal preparation, So-Cheong-Ryong-Tang (SCRT). The presence of paeoniflorin, trans-cinnamic acid, schisandrin and glycyrrhizin in this decoction was ascertained by retention time, spiking with each authentic standard, UV spectrum and ESI mass spectrum. All four compounds showed good linearity ($r^2$ > 0.998) in a relatively wide concentration ranges. The RSD for intra-day and inter-day precision was less than 3% and the limits of detection (LOD) were less than 30 ng. The mean recovery of each compound was 94.1-113.0% with RSD values less than 3.0%. These results suggest that the developed HPLC method is simple, effective and could be readily utilized as a quality control method for commercial SCRT products.

Overview and Recent Development of Recycling Waste Refrigerators (폐(廢) 냉장고(冷藏庫) 재활용(再活用) 현황(現況)과 기술(技術) 전망(展望))

  • Yang, Hyunseok;Kim, Geon-Hong;Kong, Man-Sik;Park, Kiejin;Lee, Gwang Weon;Kim, Bo Saeng
    • Resources Recycling
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    • v.22 no.4
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    • pp.70-80
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    • 2013
  • Waste refrigerator is the most large amount of item being recycled and the recycling process is the most complicated in WEEE (Waste Electrical and Electronic Equipment) because refrigerator is biggest product and consists of various parts and materials such as ferrous, non-ferrous, and plastics. Recently, recycling process of waste refrigerator has been being more complex since large capacity 2 door refrigerators and standing Kimchi refrigerators with various material are distributed on custom market. In addition, recycling of valuable resource from waste refrigerator is mandatory by WEEEs recycling legislation; therefore, high efficiency recycling enough for economic and environment-friendly recovery of valuable resource through present technical situation analysis and comparison of recycling technologies of waste refrigerator with advanced country.