• The Journal of Industrial Distribution & Business
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• v.9 no.12
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• pp.45-53
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• 2018

Purpose - The purpose of the study was to investigate operation system of current convenience store and competing retail store and to find out development of unattended convenience store. Operators can save labor cost and solve labor seeking when opening shop under suitable environment and commerce area, and assure of employee's safety. Customers are allowed to select commodity comfortably without disclosure of face and to shorten standby time at simple store opening, buying and paying and to produce convenient living environment by financing, delivery and goods control. This study examined effect and problems of operation of existing and unattended convenience store from point of view of visual merchandising to find out model of unattended convenience store and to strengthen competitiveness of convenience store business. Research design, data, and methodology - Research procedure of accomplishment of goal of the study was: The study investigated state of distribution business, and role and definition of VMD. First, VMD played an important role of promotion and competitiveness of unattended convenience stores. Second, the author investigated research and cases of unattended convenience stores 14 months, that is to say, from August 15, 2017 to October 15, 2018. Third, the author investigated on-the-spot three of common convenience stores, three of unattended convenience stores and three of retailers one month, that is to say, June 1, 2018 to July 1, 2018. The subject was convenience stores and retailers selling similar type of products at Seoul and metropolitan area. Results - Layout, selling place, brand identity, goods assortment, authentication system, product information cognition and control, buying and payment system, security system, salesmen, promotion and other services should be improved from point of view of merchandising. Structure, furniture, lighting, voice, façade, VP, PP, IP and POP should be developed. Conclusions - Unattended convenience stores saved operating cost and lessened labor and gave visitors convenience and comfortable shopping and made use of resources effectively. Further studies with quantitative analysis shall find out strategic conditions for promotion of unattended convenience stores.

• Microbiology and Biotechnology Letters
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• v.30 no.4
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• pp.312-319
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• 2002

The Sporosarcina psychrophilia pyrB gene, which encodes aspartate transcarbamylase (ATcase), was cloned on Sau3AI restriction endonuclease fragment inserted into pUC19 plasmid vector, S. psychrophilia pyrB gene was expressed in Escherichia coli pyrB mutant for the complementation test. The sequence of 2,606 nucleotides including putative pyrB gene was determined. The region contained one full open reading frame (ORf) and two partial ORFs. The deduced amino acid sequence of the second ORF showed 59% identity with that of Bacillus caldolyticus ATCase. The first and third partial ORFs were closely related to the uracil permease (pyrP) and dihydroorotase (pyrC), respectively. Besides, potential terminator, antiterminator, and anti-antiterminator structures were found in the intergenic region between pyrP and pyrB. These results suggested that S. psychrophilia pyrimidine nucleotide biosynthesis genes are clustered as well as other Bacillus sp. Over-expressed product of pyrB encoding ATCase was purified and analyzed by the SDS-PAGE. The purified PyrB protein turned out to be molecular mass of 27 kDa and showed ATCase activity.

• Journal of Aquaculture
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• v.6 no.3
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• pp.221-233
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• 1993

Cell size, DNA content, chromosome and PCR-based mitochondrial 12S rRNA gene analyses were conducted to obtain basic informations for genetic stock identification of spotted flounder (Verasper variegatus) from Yeocheun, Korea. The mean erythrocytic and nuclear volumes of spotted flounder were $211.10{\mu}m^3$ and $23.03{\mu}m^3$, respectively. The haploid DNA content of this species was 0.79 pg/cell which correspond to $46.5\%$ of carp and to $22.6\%$ of mammals. Spotted flounder had the 2n = 46 acrocentric chromosomes but no heteromorphic sex chromosomes was found. Mitochondrial DNA gene for 12S ribosomal RNA was amplified by polymerase chain reaction (PCR) and the PCR products were subjected to digestion with 15 restriction endonucleases. Restriction enzyme analyses revealed that Ava I, Mae II, Sma I and Xba I had one restriction site in the mitochondrial 12S rRNA gene segment of spotted flounder, while Mae I had two. Segments of 12S rRNA gene from mitochondria in spotted flounder were sequenced and compared with channel catfish and human as controls. The 12S rRNA gene of this species was more similar to that of channel catfish than to human's.

• The Journal of the Korea Contents Association
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• v.14 no.10
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• pp.583-595
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• 2014

Hospitals in Korea have been increasingly using physician assistants (PA) as an alternative way of dealing with the shortage of residents. However, some incidents of a Physician's Assistant practicing beyond their legal scope require closer examination of the current PA's roles and functions. This study is a web-based survey designed towards targeting physician assistants in Korea (KPA) who practice delegated tasks under a physician's license. Currently, there are 2,125 KPAs working in 141 general hospitals and medical centers. Data from 704 nurses from who responded to the questionnaire were analyzed with descriptive statistics using the SPSS 12.0 program. Their mean age is 32.5 years with 8-10 years of clinical experiences, with males being more likely to be a PA. Despite of KPAs providing medical services and performing invasive procedures, only 13% of KPAs are licensed APNs (advanced practice nurse). KPAs have a low job satisfaction due to a lack of rewards and the necessity for providing illegal practices, and are experiencing identity confusion. The current KPA system is a transitional product of the change from the hierarchial structure to a more collaborative relationship between the medical and nursing departments. Providing adequate education and training, establishing protocols with legal protection, and developing professional independent scope of care are recommended to deliver safe and efficient medical services.

• Clean Technology
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• v.13 no.4
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• pp.287-292
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• 2007

[ $M/Al_2O_3$ ] (M = Mn, Fe, Co, Ni, Cu) catalysts supported on commercial alumina ($Al_2O_3$) were prepared by an impregnation method, and were applied to the hydrogen production by auto-thermal reforming of ethanol. It was revealed that each catalyst retained its own metallic phase and product distribution strongly depended on the identity of active metal. Among the catalysts prepared, $Ni/Al_2O_3$ and $Co/Al_2O_3$ showed the best catalytic performance in the auto-thermal reforming of ethanol. However, the reaction mechanisms over these two catalysts were different. Ni/Al_2O_3 catalyst showed 100% ethanol conversion at $500^{\circ}C$, but it exhibited a rapid decrease in hydrogen selectivity. Although $Co/Al_2O_3$ catalyst showed an excellent performance in hydrogen selectivity, on the other hand, no significant improvement in hydrogen yield was observed due to the low ethanol conversion over the catalyst.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.5
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• pp.736-742
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• 2017

Objective: Experiments were conducted to clone the sequence of Wild Argali short palate, lung and nasal epithelium clone 1 (SPLUNC1) cDNA, and to lay the foundation for further study the biological function of Wild Argali SPLUNC1. Methods: The complete sequence of Wild Argali SPLUNC1 cDNA was generated by rapid amplification of cDNA ends. The entire coding sequence was inserted into the pPIC9K vector and expressed in Pichia pastoris (P. pastoris) GS115. The recombinant SPLUNC1 protein was detected by Western blot and purified by $Ni^{2+}$ chelate affinity chromatography. The test of effect of the protein on Mycoplasma ovipneumoniae (MO) was performed with real-time polymerase chain reaction. Results: The Wild Argali SPLUNC1 cDNA was 1,076 bp with an open reading frame of 768 bp, which encoded a 26.49 kDa protein composed of 255 amino acids. Its amino acid sequence shared 98.4%, 96.9%, 94.5%, 90.2%, 80.8%, 78.4%, 78.3%, 72.5%, 72.3%, 68.8% identity with those of SPLUNC1 cDNA from Ovis aries (accession no. NP_001288334.1), Capra hircus (accession no. XP_005688516.1), Pantholops hodgsonii (accession no. XP_005979709.1), Bos taurus (accession no. NP_776851.1), Felis catus (accession no. XP_006929910.1), Homo sapiens (accession no. NP_001230122.1), Sus scrofa (accession no. NP_001005727.1), Chinchilla lanigera (accession no. NP_001269294.1), Mus musculus (accession no. NP_035256.2), and Rattus norvegicus (accession no. NP_742028.1), respectively. The recombinant protein corresponded to the expected molecular mass of 25.47 kDa as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it was detected in the supernatant of P. pastoris, and it could be purified. The results from the test of inhibition effect of argali recombinant SPLUNC1 protein on MO showed that the product could inhibit MO very well (p<0.01). Conclusion: The amino acid sequence of Wild Argali SPLUNC1 was different from other organisms. The recombinant SPLUNC1 protein has good biological activity.

• The Journal of the Korea Contents Association
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• v.19 no.11
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• pp.664-675
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• 2019

This study examined Korean sign Language of the linguistic form and the etymological forms about the origins of the Korean national sign languages. The general sign language has been shown through previous research all of place names from Chinese character except Seoul and Lmsil. And then, Sign language's form and origins which are current using in order to examine what kind of feature were analysed through interviews and publications in Korean association of the deaf people. As a result, it was analysed that was composed majority. indigenous sign language Korean place names were made and used by deaf than loan word character of Chinese characters, Hangul and loanword. When we consider that place names were correspond to a precious cultural heritage, representing the history with the culture and identity of the relevant area, we can worth of preservation and transmission to the abundant iconicity in the name of Sui. On the other hand the indigenous sign language korea place manes can worth deaf culture or korean sign language. Even lf geographical characteristics of area have been changed or local product was disappeared in this situation by The origin of sign language reach in modern time local specialty by geographical form lt continued over generation. This can be regarded as the Korean sign language of the form in the way of visual. lt will be very valuable heritage in the preservation deaf culture.

• Journal of the Mineralogical Society of Korea
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• v.19 no.3 s.49
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• pp.129-138
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• 2006

In this study, we identified the secondary precipitates from Samsan-jeil and Sambong mine, Goseong, Gyeongnam by means of scanning electron microscopy, electron probe microanalysis and X-ray powder diffraction analysis. Copper sulfide minerals had been produced from the mines during last few decades, however they are not worked. White and blue precipitates were found at the downstream of mine rock dump at Sambong mine and green one was at Samsan-jeil mine. The white precipitate covered the host rock surface with thickness of $30{\mu}m$, and is a kind of diatom with $10{\mu}m$ in length and $3{\mu}m$ in width. It is a species Fragilaria constuens, which is contained a order Pennales(pennate diatom) and lives in fresh water. The blue precipitate is the alteration product of chalcopyrite. It resultes in the increase in the ratio Cu:Fe from 5 to 13. The green precipitate has worm-like morphology with $10{\sim}20nm$ in diameter and $200{\sim}300nm$ in length. It is mainly composed of secondary copper sulfate such as woodwardite. However, it could be formed by the activity of microorganism, because the copper content is more than any secondary copper sulfate reported in copper sulfide mine. In order to identity the green precipitate exactly, the further research is needed.

• Korean journal of communication and information
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• v.57
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• pp.84-108
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• 2012

This article is concerned with exploring labour market transformation in newspaper industry and thereby looking into skill formation and professionalization of journalists. In Korea, according to previous research, newspaper journalist labour market had been characterized by a low inter-firm mobility of workers and patterns of long-term employment and firm-based skill training. However, over the past few years, as new product strategies of newspaper firms has changed due to management crisis employers pull back from the responsibilities of investment on skill training and securing job stability for journalists. However, in spite of overall weakened firm-based skill formation systems within the market, there still seems to be long way to build alternative systems of skill developments for them. As a result, it will be argued that, in the absence of manifest labor market institutions such as apprenticeships or skill certification system, which are assumed to traditionally certify workers' professionalization, occupational identity and professionality of newspaper journalist labour market will be weakened. Labor market data from interviews with journalists in the newspaper industry are used in order to test this hypothesis.

• The Journal of the Korea Contents Association
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• v.20 no.1
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• pp.324-333
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• 2020

This study compares the visual elements of the top five global beer brands currently in the Chinese market with those of the top five Chinese beer brands. The purpose of the research is to identify the importance of visual elements in the label design of beer package and to present the direction of efficient label design. To this end, it is a brand among visual elements of beer label design, and it investigates the label design of Chinese beer and global beer brand through the multi-accelerated comparative analysis method, focusing on color, illustration and layout, and analyzes the difference of elements appearing in the global beer label design from China by utilizing the recursive five-point scale. According to the research, the visual elements used in the Chinese beer brand were more complex compared to the global beer brand, and most layouts were similar, making the product less differentiated. On the other hand, the global beer brand has secured brand identity and differentiation based on its iconic visual elements and diversity of layout. In order to establish itself as a strong brand in consumers' minds, the Chinese beer brand needs differentiated label design through active use of unique brand design.