• 제목/요약/키워드: Producer analysis

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Isolation and Characterization of the Biosynthetic Gene Clusters for Aminoglycoside Antibiotics

  • Jung Yong-Gyun;Jo You-Young;Hyun Chang-Gu;Lee In Hyung;Yang Young-Ye1l;Suh Joo-Won
    • 한국미생물학회:학술대회논문집
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    • 한국미생물학회 2001년도 추계학술대회
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    • pp.146-156
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    • 2001
  • The biosynthetic gene clusters for bluensomycin and spectinomycin were isolated and characterized from the bluensomycin producer, Streptomyces bluensis ATCC27420 and the spectinomycin producer, Streptomyces spectabilis ATCC27741, respectively. PCR primers were designed specifically to amplify a segment of dTDP-glucose synthase gene based on its conserved sequences of several actinomycete strains. By screening cosmid libraries using amplified PCR fragments, 30-kb and 45-kb DNA fragments were isolated from Streptomyces bluensis and Streptomyces spectabilis, respectively. Sequencing analysis of them revealed that each contains 15 open reading frames (ORFs). Some of these ORFs were turned out to be antibiotic resistance genes (blmA and speN), dTDP-glucose synthase genes (blmD and spcD), and dTDP-D-glucose 4,6-dehydratase genes (blmE and spcE), suggesting that the blm and spec gene clusters are likely involved in the biosynthesis of bluensomycin and spectinomycin, respectively.

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20세기 한국 남성 성역할에 따른 남성복 디자인 변화 특성 연구 (Diachronic Analysis of Korean Men's Wear Design based on Changes in Gender Roles)

  • 이지현
    • 복식
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    • 제60권8호
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    • pp.51-66
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    • 2010
  • This study aimed to analyze the changing characteristics between Men's Gender Role and Men's wear Design in 20th century. To analyze the representative Men' Gender roles, the related studies and literatures reviewed. Literature reviews and quantitative studies were performed to analyze the changing differences of Korean Men's wear Deign as a symbolic factors of gender role. The results of study are as follows. 1) The representative men's gender roles in 1900's~1920's were 'Patriarchal/ Stern Man'. The narrow V-zone of jacket and shoulder, high shirt collars and well fitted suits expressed the stern images of that times. 2) In 1930's~50's, 'Enervated/ Escapist Man' were the representative images. They were expressed with wide lapels and shoulders, emphasized V-zone and abundant trousers. Especially 40's style was under the influence of American 'Zoot Style'. 3) The representative gender roles were the men of 'Producer/Provider' in 60's~70's. Shortened jacket, wide lapels & neckties, slim trousers were used to express the strong & young images of Producer/ Provider during the industrialization era. 4) The representative gender roles were 'Attentive/ Intellectual/ Beautiful Men' in 80's~90's. The exaggerated round shoulders which were influenced by 'Big Look', and easy silhouette were related with the attentive images.

Nucleotide sequence analysis of a second set of the polyketide synthase .betha.-ketoacyl synthase and chain length factor genes from the salinomycin-producing streptomyces albus

  • Hyun, Chang-Gu;Park, Kwan-Hyung;C.Richard Hutchinson;Suh, Joo-Won
    • Journal of Microbiology
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    • 제35권1호
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    • pp.40-46
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    • 1997
  • The pWHM220 cosmid with a 24-kb insert cloned from Streptomyces albus ATCC 21838 induces the biosynthesis of a polysther antibiotic similar to salinomycin in Streptomyces invidans. We have analyzed this region by DNA sequencing as well as Southern blot hybridization with type I and type II polyketide synthase (PKS) probes. Surprisingly, we found another set of type II SKS genes only 10-kb from the original PKS genes, salABCDE. The DNA sequence revealed two complete open reading frames (ORFs) named salB2 and salC2, and one partial ORF that does not resemble any known DNA or deduced protein sequence. The salC2 should code for chain length determining factor while the deduced amino acid sequence encoded by salB2 exhibits high similarity to .betha.-ketoacyl synthase from different PKS gene clusters. The highest identity was found for .betha.-keetoacyl synthases from S. argillaceus (MtmP. 59.1% identity), the mithramycin producer and from S. venezuelae ISP5230 (JadA, 52.3% identity), the jadomycin producer. The SalC2 protein clearly resembles its counterparts in order aromatic PKS gene clusters that are believed to influence the length of the polyketide chain. The highest identities observed were to that of S. argillaceus (MtmK, 62.3%) and S. venezuelae ISP 5230 (JadB, 55.1%) proteins, Moreover, the deduced amino acid sequences of the salB2 and salC2 products were 29.0% identical.

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Market Efficiency Analysis between Facility-Based and Service-Based Competition

  • Seo, Il-Won;Lee, Duk-Hee;Kim, Byung-Woon
    • ETRI Journal
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    • 제30권4호
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    • pp.587-596
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    • 2008
  • Facility-based competition (FBC) in the telecommunications market is considered to have lower static efficiency in the short term and higher dynamic efficiency in the long term. Under service-based competition (SBC), the entrant can reduce its setup costs by leasing network facilities from the incumbent, which makes the entrant viable, pushes the market price down and promotes static efficiency. This paper attempts to measure static efficiency by comparing the profits of the incumbent and entrant in terms of consumer surplus and social welfare under each competition type by extending the Stackelberg model. The results, assuming a linear demand function and variation in regulatory level, show that FBC results in higher social welfare than SBC on the whole. However, SBC accompanied by strong regulation is also shown to have the potential to be superior over FBC. It is also revealed that FBC exhibits a higher producer surplus (particularly, the incumbent's producer surplus) and is, therefore, more desirable in terms of dynamic efficiency. When the entrant's cost is high in FBC, social welfare is shown to be lowered, implying that cost competitiveness is a necessary condition for social welfare.

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거시경제변수를 고려한 기술평가모형의 개선 : 기술보증기금의 사례 (An Improved Technology Appraisal Model Considering Macroeconomic Variable : A Case of KOTEC)

  • 김대철;김재범;조근태
    • 경영과학
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    • 제30권2호
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    • pp.117-132
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    • 2013
  • The objective of this paper is to provide an improved technology appraisal model, which considers a variety of macroeconomic variables such as consumer price index and producer price index. The improved model was built using cross correlation analysis and logistic regression analysis. The AUROC analysis showed that goodness-of-fit of the proposed model turned out to be improved than that of the existing model. The model proposed in the paper would be helpful for making a reasonable investments and financing decision, lessening the default rates by systematic risk management, and enhancing the technology commercialization capabilities.

원예부문 연구 및 지도 사업의 투자효과 분석 (Returns to Investment on Research and Extension in Korean Horticulture)

  • 강경하;이민수;최영찬
    • 농촌지도와개발
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    • 제7권2호
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    • pp.257-277
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    • 2000
  • The objectives of this study are to investigate the relationship between the growth of the horticultural sector and horticultural research and extension and to examine the socioeconomic returns to investment on research and extension in Korean horticulture. Data for horticultural production values, producer price indices and research and extension budgets for horticultural sector from 1965 to 1998 are collected from various sources. Multi-variate time series analysis technique with vector auto-regression model and Akino-Hayami Formula were employed for the analysis. This study finds (1) horticultural production responds about seven years later to the horticultural research investment shock. the magnitude of the impacts increases to a peak in seventeen years from the initial expenditures and then declines slowly thereafter until twenty years. and this peak gives a tip that horticultural research impact lasts much longer than grain's or agriculture's: (2) the social surplus from research investment benefits more to the consumer rather than to the horticultural producer: (3) B/C ratios in horticultural research are quite high with the range of 9 to 55 from 1965 to 1998. but these have been decreased since the early 1990s: (4) the socioeconomic returns to horticultural research is quite high with 56 percents of internal rate of return. It remains to be analyzed returns to investment on extension in horticulture because of no statistic significance in this study.

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Assessment & implications of the business cessation support system for farmers: focus on the grape business

  • Han, Sukho;Youm, Jungwon;Jang, Heesoo;Koo, Seungmo
    • 농업과학연구
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    • 제47권3호
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    • pp.533-544
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    • 2020
  • In this paper, we conducted a dynamic ex-post assessment on the grape business cessation support System. Based on the analysis results, in the short term, there was an increase in grape price due to a decrease in production and accompanying increases in the prices of consumption substitution items. However, in the long run, grape prices fell again due to an increase in grape production because of the entry of new grape farmers and the growth of adult grape trees. In addition, the analysis showed that the balloon effect caused by the conversion of crops caused indirect damage such as an increase in the production volume of substitution crops and a decrease in prices. When analyzing the social welfare measurement, the results showed that the support system for business cessation increased the overall social welfare due to an increase in producer welfare because of a price increase in the short term, but in the long term, both producer and consumer welfare decreased. In the end, it is necessary to review the system because the government's intervention may cause market distortion and inefficient resource allocation. Above all, it is necessary to minimize the indirect effect of the industry's contraction and balloon effect due to excessive business cessation. For this, conditional support should be provided in parallel with post management rather than unconditional support. In addition, it is necessary to provide a strategic support system that considers substitution items in addition to those items to be supported.

친환경농산물에 대한 구매행태 특성분석 -광주광역시 소비자를 중심으로- (An Analysis on the Characteristics of Purchasing Behavior for Environment-friendly Agri-products on Consumers in Gwangju)

  • 안병렬
    • 한국유기농업학회지
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    • 제13권3호
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    • pp.229-242
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    • 2005
  • This study was examined to analyze of the characteristics analysis of purchasing behavior for environment-friendly agri-products on consumers in Gwangju. The 310 subjects formatted questionnaires were analyzed to frequency, percentage, crosstabulation, chi-square test and logistic regression by SPSS WIN 10.0. The main results of this study were as followings (1) It was analyzed that consumers have had the reason to purchase, purchase places, purchase number of times, purchase background, optimum price and purchase item for environment-friendly agri-products. (2) It was tended that consumer showed degree of satisfaction, point to improve and purchase intention continuously for environment-friendly agri-products. (3) It was proved that age, income, medium contact level, cognitive level, valuation basis, producer and the direct deal, the consumer organization's co-op, large-sized discount hoarded goods for purchase places and price reduction for promotion of purchase for environment-friendly agri-products was of the characteristics analysis.

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인체를 대상으로 하는 혈허 혈어증 모델 개발을 위한 손톱 주름살의 모세혈관에 대한 영상장치 연구 ( I ) (Study on the Image Analysis System of Nail-fold Capillary Vessel( I ))

  • 김경철;황원덕
    • 동의생리병리학회지
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    • 제18권3호
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    • pp.789-791
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    • 2004
  • We study on the video-capillaroscopy system for look a.t the nail fold capillary by the computer. This system is composed of the luminous source-producer, image-input, phase microscope. The method and contents of observation on this system are seperated from the image-analysis of nail fold capillary form-patterns and the currents of hematocytes. Therefore we think this system is possible to the practical clinic use for the basic model on the cardiac failure and the deficiency(血虛), stagnant(血瘀) of blood in human.

Cellulase 유전자 염기서열에 기초한 Sorangium cellulosum 균주들의 계통분류 (Phylogenetic Analysis of Sorangium cellulosum Strains Based on Cellulase Gene Sequences)

  • 이한빛;윤진권;조경연
    • 한국미생물·생명공학회지
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    • 제39권1호
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    • pp.20-28
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    • 2011
  • 두 개의 cellulase 유전자 xynB1, bglA2과 groEL1 유전자 염기서열에 기초하여 국내에서 분리한 34균주의 Sorangium cellulosum 균주들을 계통 분석한 결과, 점액세균 중 가장 많은 생리활성물질이 발견된 종인 S. cellulosum 내에는 최소한 5그룹의 소그룹이 존재함을 보였다. 이 분석은 또한 S. cellulosum 균주들의 다양성을 보여주어 분석한 34균주 중 30균주가 서로 다른 균주인 것으로 나타났다.