• 한국식품영양과학회지
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• 제45권8호
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• pp.1227-1232
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• 2016

본 연구에서는 다양한 온도의 물로 제조한 전해수를 이용하여 그람양성균과 그람음성균에 대한 살균력을 확인하였다. 전해수의 물성은 물의 온도가 높아질수록 pH와 유효 잔류염소 농도 값이 높아졌으나, 산화환원전위 값은 감소하였다. 4, 22, $40^{\circ}C$의 물을 이용하여 생성한 전해수에 Escherichia coli O157:H7을 15분 동안 처리하였을 때 각각 1.18, 4.47, 5.46 log CFU/mL 감소하였으며, Staphylococcus aureus는 각각 0.72, 4.90, 5.54 log CFU/mL 감소하였다. 이를 통해 전해수를 생성하는 물의 온도가 증가할수록 살균 효과가 높아지는 것을 알 수 있다. 4, $40^{\circ}C$의 물을 이용하여 생성한 전해수에 균주를 처리한 경우, Listeria monocytogenes의 D 값(일정한 온도에서 90 %의 미생물이 사멸하는 데 걸리는 시간)이 각각 6.60, 1.57분으로 나타나 가장 낮았으며, $22^{\circ}C$ 전해수에서는 Salmonella Enteritidis의 D 값이 2.92분으로 가장 낮은 값을 나타내었다. 그람양성균과 그람음성균을 비교하였을 때 모든 온도에서 D 값에 대한 유의적인 차이는 나타나지 않았다(P>0.05). 본 연구 결과는 전해수를 제조할 경우 높은 온도의 물을 이용하여 제조하는 것이 미생물 살균 효과가 높다는 것을 보여주고 있다. 따라서 전해수를 생성할 때 물의 온도를 고려하여 높은 살균력을 나타내는 전해수를 생성할 수 있으며, 이는 물의 온도 외에도 물의 경도나 물의 종류 등을 고려하여 최적의 살균 전해수를 제조함으로써 식품산업에 적용하기 위한 자료로 활용될 것으로 기대된다.

• 한국식품과학회지
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• 제46권1호
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• pp.33-38
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• 2014

발효 전 가수량에 따른 복분자 와인의 품질 특성을 분석하기 위해, 복분자 중량 대비 0배(A), 1배(B), 2배(C), 3배(D) 그리고 4배(E)의 물을 첨가하여 발효를 진행하였다. 가수량이 증가할수록 초기 pH는 높았지만, 발효 후에는 오히려 pH가 낮은 값을 보였다. 총산도는 가수량이 증가할수록 점차 감소했지만, 감소되는 비율은 적었다. 가수량이 증가할수록 발효 속도가 느려졌지만, 최종 알코올 함량은 높았다. 가수를 하지 않은 복분자 와인(A)에 비해 가수를 하고 제조한 복분자 와인(B, C, D, E)의 복분자 원료당 추출되는 총 폴리페놀 함량은 2.86-4.42배 높았으며, 총 플라보노이드 함량은 2.58-3.01배 높았다.

• 대한미생물학회지
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• 제35권1호
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• pp.77-85
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• 2000

Streptococcus mutans is the most important causative bacteria of dental caries among the oral bacteria. Lactococcus lactis 1370 was isolated from the oral cavity of child. The effect of Lactococcus lactis 1370 on the formation of artificial plaque by Streptococcus mutans was studied. 1. The insoluble substances and bacteria were much more attached on the wall of disposable cuvette in the culture of Streptococcus mutans than in the combined culture of Streptococcus mutans and Lactococcus lactis 1370. 2. The mean weight of produced artificial plaque on the wires in the beaker was 131.7 mg in the culture of Streptococcus mutans only, whereas being reduced to 6.4 mg in the combined culture of Streptococcus mutans and Lactococcus lactis 1370 (p<0.05). The viable cell didn't show the significant difference between them after culturing. 3. When Streptococcus mutans was cultured in the media containing culture supernatant of Lactococcus lactis 1370 cultured in M17 broth containing 0.5% yeast extract and 5% sucrose, the mean weight of produced artificial plaque was 8.0 mg on the wires, whereas being 125.4 mg in the media without culture supernatant of Lactococcus lactis 1370 (p<0.05). The viable cell didn't show the significant difference between them after culturing. 4. When Streptococcus mutans was cultured in the media containing soluble polymer produced by Lactococcus lactis 1370, the mean weight of produced artificial plaque was significantly reduced compared with being cultured in the media without soluble polymer (p<0.05). The viable cell didn't show the significant difference between them after culturing. 5. The soluble polymer produced by Lactococcus lactis 1370 was glucan. 6. The glucan produced by Lactococcus lactis 1370 was water-soluble glucan containing ${\alpha}$-1,6-glucose linkage as the main linkage. These results suggest that the artificial plaque formed by Streptococcus mutans is inhibited by water-soluble glucan produced by Lactococcus lactis 1370.

• Korean Journal of Acupuncture
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• 제28권3호
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• pp.33-41
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• 2011

Objectives : The purpose of this study is to investigate effects of Scutellariae Radix water extract on hydrogen peroxide production in LPS-induced RAW 264.7 mouse macrophages. Methods : Scutellariae Radix produced from South Korea (SK) and Scutellariae Radix produced from China (SC) were extracted by hot water. Effects of SK and SC on hydrogen peroxide production in LPS-induced RAW 264.7 were measured by dihydrorhodamine 123 assay after 20, 24, 28, 44, 48, and 52 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL. Results : SK significantly increased hydrogen peroxide production in LPS-induced RAW 264.7 cells for 20, 24, 28, 44, 48, and 52 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL (P < 0.05). But SC did not represent any significant effect on hydrogen peroxide production in LPS-induced RAW 264.7 cells. Conclusions : These results suggest that Scutellariae Radix, especially produced from South Korea, has the immune-enhancing property related with its increasement of bacteriocidal hydrogen peroxide production in LPS-induced macrophages.

• 아시안잔디학회지
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• 제12권4호
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• pp.211-220
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• 1998

Cellulose-degrading fungi were idenfied as Rhizoctonia solani AG2-2(IV), T. harzianum and C. cochliodes. Rhizoctonia solani AG2-2(IV) grows better in the acidified media of pH 4 and 5 than pH 6 and 7. Mycelial growth of T. harzianum and C. cochliodes was also higher in pH 4 and 5 than in pH 6 and 7. In order to relate the above findings to nutrient utilization, mycelial growth of R. solani AG2-2(IV) are evaluated with various carbon sources. R. solani AG2-2(IV) grows well in the order of mannose, cellobiose, glucose, xylose and arabinose. However, mycelial dry weights of T. harzianum were 98.7, 78.0, 72.3, 43.7 and 32.3mg in glucose, mannose, cellobiose, xylose, and arabinose, respectively. Mycelial dry weight of C. cochilodes was 118, 65, 57, 49, and 16mg in mannose, cellobiose, xylose, glucose, and arabinose, respectively. Result of cellulase assay of R. solani AG2-2(IV) and soil fungi was reffered as, R. solani AG2-2(IV) produced more cellulase on CMC substrate than on CEL and secretes more enzyme in floated condition than in water-immersed condition. T. harzianum secreted less amount of cellulase than R. solani AG2-2 and C. cochliodes. T. harzianum produced no enzyme on CEL under water-immersed condition. C. cochliodes produced similar amounts of cellulase on either CMC or CEL under both water-immersed and floated condition.

• 한국구조물진단유지관리공학회 논문집
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• 제24권3호
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• pp.57-62
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• 2020

국내에 설치된 하수관거 중에서 20년을 초과한 콘크리트관의 연장은 66,334km로서 전체의 42.5%를 차지한다. 열화된 콘크리트 하수관거에서는 내부 하수의 유출로 인해 관로 주변의 동공을 확장시켜 땅꺼짐 등의 문제도 발생시키기 때문에 이에 대한 보수를 통한 유지관리가 필요한 실정이다. 본 연구에서는 초속경시멘트 및 분리저감제를 적용한 수중불분리성 모르타르를 하수관거 보수에 적용하기 위한 기초연구를 수행하였다. 배합조건에 따른 응결시험 결과 분리저감제 혼입에 따라 종결이 최대 172%정도 지연되는 것으로 나타났다. 수중분리도는 분리저감제 0.3% 이상 혼입시 pH 12 이하로 측정되었고, 현탁액량은 분리저감제 0.2% 이상 혼입시 50mg/l이하로 측정되어 국내 KCI-AD102 기준을 만족하였다. 기건조건과 수중조건에서 타설하였을 때의 강도비 평가 결과, 분리저감제 혼입시 80% 이상의 강도비를 나타내어 국내 KCI-AD102 기준을 만족하였다. 부착강도는 분리저감제 혼입시 기건조건보다 수중조건에서 타설하였을 때 높은 강도로 측정되었다. 내수성 검토는 분리저감제 혼입량에 따른 흡수율과 투수성으로 평가하였고, 각각 42.6%, 36.6%의 성능개선을 확인하였다.

• 공업화학
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• 제10권1호
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• pp.173-176
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• 1999

초임계수를 이용한 금속산화물의 미세입자 제조에 대하여 연구하였다. 금속염 수용액으로는 cobalt nitrate solution과 manganese nitrate solution을 sample solution으로 선택하여 코발트산화물과 망간산화물 입자를 제조하였다. 얻어진 결과로부터 초임계수를 이용한 금속산화물 미세입자의 제조가 가능함을 확인할 수 있었으며, 초임계수 하에서는 매우 빠른 dehydration반응이 일어남을 관찰할 수 있었다. 짧은 반응시간(30~100 초)에도 불구하고 미세입자 ($0.5{\sim}2{\mu}m$)가 생성되었으며, 초임계수 공정에서는 mixer의 온도가 입자의 크기 및 분포에 큰 영향을 미침을 알 수 있었다. 반응온도 조절을 통하여 입자의 크기를 제어할 수 있음을 확인할 수 있었다.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2008년도 춘계학술대회 논문집
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• pp.206-209
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• 2008

The modified supercritical water treatment method is adopted for hydrolysis of wood powder, Populus alba$\times$glandulosa. This modified method is containing 0.05% HCl or $HNO_3$ as acid catalyst. The supercritical water treatment(SCW) was performed for 1 min. with $350^{\circ}C$, $380^{\circ}C$, $400^{\circ}C$ and $425^{\circ}C$, respectively, under 230 $\pm$ 10 atm using continuous flow system. When acid was added to powder prepared for SCW treatment, the yields of monomeric sugars were significantly increased. The lignin remained after supercritical treatment was applied to gel permeation chromatography(GPC) for molecular weight distribution analysis. Compared to the lignin produced from SCW treatment without acid catalyst, the average molecular weight of lignin compounds treated with acid was clearly decreased. Particularly, Mn/Mw ratio is decreased. This result shows supercritical water treatment of wood powder can change the molecular weight of lignin to small size. However, it is necessary to be further studied for exactly characterizing the lignin produced from supercritical water treatment.

• Journal of Applied Biological Chemistry
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• 제53권3호
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• pp.170-173
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• 2010

Antioxidant and anticoagulant activities of water extract and 70% ethanol extract of Phyllostachys pubescence leaf (PPL) produced in Geoje were investigated. Total polyphenol contents of PPL-water extract and PPL-70%, EtOH extract were measured as $80.6{\pm}3.2$ and $71.3{\pm}2.7$ mg/g, respectively. DPPH radical scavenging activity of PPL-water extract and PPL-70% EtOH extract were 50% and 90%, respectively. The anticoagulant activity, in intrinsic pathway of activated partial thromboplastin time (APTT) showed a concentration-dependency. This results showed that PPL-water extract and PPL-70% EtOH extract have high antioxidant and anticoagulant activities.

• Bulletin of the Korean Chemical Society
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• 제8권5호
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• pp.414-418
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• 1987

Electrochemical reduction of dibenzoylmethane was studied on mercury electrode by means of cyclic voltammetry, polarography and potentiostatic measurements in ethanol-water system. In acidic solutions monomeric pinacol was produced by irreversible two-electron process while monomeric and dimeric pinacol were competitively produced by the same process in neutral solution. However, in basic solution the dimeric pinacol was mostly produced through radical by irreversible one-electron transfer process. Mechanisms of the reduction of dibenzoylmethane are deduced from Tafel slope, pH dependance and reaction order with respect to the concentration of dibenzoylmethane in the solution of various pH.