A seagrass that is marine vascular plants plays an important role in the marine ecosystem, so periodic monitoring ofseagrass habitatsis being performed. Recently, the use of dronesthat can easily acquire very high-resolution imagery is increasing to efficiently monitor seagrass habitats. And deep learning based on a convolutional neural network has shown excellent performance in semantic segmentation. So, studies applied to deep learning models have been actively conducted in remote sensing. However, the segmentation accuracy was different due to the hyperparameter, various deep learning models and imagery. And the normalization of the image and the tile and batch size are also not standardized. So,seagrass habitats were segmented from drone-borne imagery using a deep learning that shows excellent performance in this study. And it compared and analyzed the results focused on normalization and tile size. For comparison of the results according to the normalization, tile and batch size, a grayscale image and grayscale imagery converted to Z-score and Min-Max normalization methods were used. And the tile size isincreased at a specific interval while the batch size is allowed the memory size to be used as much as possible. As a result, IoU was 0.26 ~ 0.4 higher than that of Z-score normalized imagery than other imagery. Also, it wasfound that the difference to 0.09 depending on the tile and batch size. The results were different according to the normalization, tile and batch. Therefore, this experiment found that these factors should have a suitable decision process.
Hydrophobic core variant of ubiquitin appeared to have partially folded structure at pH around 2. The intrinsic tryptophan fluorescence emission maximum of this ubiquitin variant at pH 2 showed slight blue shift compare to that of unfolded state, suggesting that some residual tertiary structures remain in this solvent condition. At the same solvent condition, this ubiquitin variant binds with hydrophobic dye, 8-anilinonaphthalene-1-sulfonic acid(AMS), which is known to bind to exposed hydrophobic surface. Furthermore, far-UV circular dichroic spectrum of this ubiquitin variant in the diminished pH was remarkably different from the far-UV CD spectrum of the native state or unfolded state. Based on the molar ellipticity at 220 nm, this ubiquitin variant at pH 2 appeared to have significant amount of secondary structures. All these observations suggest that this ubiquitin variant in the diminished solvent pH has loosely folded hydrophobic core with some secondary structures, which are key features of molten globule conformation. Since molten globule has long been considered as a protein folding intermediate, it is considered that this hydrophobic core variant ubiquitin will serve as a valuable model to study protein folding process.
Journal of The Korean Society of Grassland and Forage Science
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v.38
no.3
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pp.196-201
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2018
Soil is the main nitrogen (N) provider for plants but N in soil is not all available to advanced plants. Mineralization is a critical biological process for transferring organic N to inorganic N that can be used by plants directly. To investigate the effect of different levels of soil temperature and water content to soil mineralization, a field experiment was established on three different sites (A, B and C). We measured soil temperature, moisture and electrical conductivity once daily after swine slurry application. Average soil moisture and temperature in site A is the highest among three sites (40.9% and $9.7^{\circ}C$, respectively). Following is in site C (37.3% and $9.6^{\circ}C$) and the lowest is in site B (28.0% and $9.0^{\circ}C$). Ammonium N (NH4+-N) and nitrate N (NO3--N) were determined on the first and fifth day after treatment. Compared with site B and C, site A always had the highest soil total N content (1.54 g N kg-1 on day one; 1.22 g N kg-1 on day five) and highest NO3-- N content (93.18 mg N kg-1 on day one; 16.22 mg N kg-1 on day five) and a significant decrease on day five. Content of NH4+-N in site B and C reduced while in site A, it increased by 6.7%. Results revealed that net N mineralization positively correlated with soil temperature (P<0.5, $r=0.675^*$) and moisture (P<0.01, $r=0.770^{**}$), suggesting that to some extent, higher soil moisture and temperature contribute more to inorganic N that can be used by plants.
Journal of the Korea Academia-Industrial cooperation Society
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v.18
no.3
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pp.518-526
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2017
This study analyzed the impact of family firms and their characteristics on how they use debts to analyze the decision-making process of Korean family firms. For analysis, we classified the characteristics of family firms into three categories, through the influence of the relationship between the lack of funds and net debt issuance, which was confirmed as the 'packing order theory' of family firms. There was a total of 4,503 enterprises in the Korean Exchange (KRX). The period of analysis was 10 years, between 2004 and 2014. To summarize, Shyam-Sunder and Myers (1999) validated the packing order theory by presenting a model of family businesses that showed greater applicable to higher packing order theory than a model of non-family businesses. Moreover, the results also confirmed the application of the packing order theory by the family stronger corporate governance and ownership structure. The ownership and governance characteristics of the ruling family has also shown the applicability of higher packing order theory.
Kim, Il-Woung;Sun, Won Suk;Yun, Bong-Sik;Kim, Na-Ri;Min, Dongsun;Kim, Si-Kwan
Journal of Ginseng Research
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v.37
no.1
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pp.124-134
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2013
The authentication of the physico-chemical properties of ginsenosides reference materials as well as qualitative and quantitative batch analytical data based on validated analytical procedures is a prerequisite for certifying good manufacturing practice (GMP). Ginsenoside Rb1 and Rg1, representing protopanaxadiol and protopanaxatriol ginsenosides, respectively, are accepted as marker substances in quality control standards worldwide. However, the current analytical methods for these two compounds recommended by Korean, Chinese, European, and Japanese pharmacopoeia do not apply to red ginseng preparations, particularly the extract, because of the relatively low content of the two agents in red ginseng compared to white ginseng. In manufacturing fresh ginseng into red ginseng products, ginseng roots are exposed to a high temperature for many hours, and the naturally occurring ginsenoside Rb1 and Rg1 are converted to artifact ginsenosides such as Rg3, Rg5, Rh1, and Rh2 during the heating process. The analysis of ginsenosides in commercially available ginseng products in Korea led us to propose the inclusion of the (20S)- and (20R)-ginsenoside Rg3, including ginsenoside Rb1 and Rg1, as additional reference materials for ginseng preparations. (20S)- and (20R)-ginsenoside Rg3 were isolated by Diaion HP-20 adsorption chromatography, silica gel flash chromatography, recrystallization, and preparative HPLC. HPLC fractions corresponding to those two ginsenosides were recrystallized in appropriate solvents for the analysis of physico-chemical properties. Documentation of those isolated ginsenosides was achieved according to the method proposed by Gaedcke and Steinhoff. The ginsenosides were subjected to analyses of their general characteristics, identification, purity, content quantification, and mass balance tests. The isolated ginsenosides showed 100% purity when determined by the three HPLC systems. Also, the water content was found to be 0.534% for (20S)-Rg3 and 0.920% for (20R)-Rg3, meaning that the net mass balances for (20S)-Rg3 and (20R)-Rg3 were 99.466% and 99.080%, respectively. From these results, we could assess and propose a full spectrum of physico-chemical properties of (20S)- and (20R)-ginsenoside Rg3 as standard reference materials for GMP-based quality control.
We have analyzed the torque signals measured in synthetic antiferromagnetic (SAF) coupled CoFeB/Ru/CoFeB thin film, which signals were drastically changed at flopping field ($H_F$) and saturation field ($H_s$). The minimum value of negative uniaxial anisotropy constant ($-\;K_1$) was appeared at HF. The $-\;K_1$ was due to the zero net magnetization by the antiferromagnetic coupling between two ferromagnetic layers. Whereas, the biaxial anisotropy constant (K2) was induced in the field range of $H_F$ < H < $H_s$. The induced $K_2$ was originated from deviation angles between magnetization directions of two ferromagnetic layers. And at H > $H_s$, intrinsic uniaxial anisotropy constant of CoFeB layer was observed. These change of the anisotropy constant with magnetic field was explained by the magnetization process of two ferromagnetic layers based on Stoner-Wohlfarth model calculation for SAF thin film.
Core-wide temperature distribution in sodium-cooled fast reactor plays a key role in its decay heat removal process, however the prediction for temperature distribution is quite complex due to the conjugate heat transfer between the assembly flow and the inter-wrapper flow. Hybrid medium model has been proposed for conjugate heat transfer modeling in the core. The core is modeled with a Realistic modeled inter-wrapper flow and hybrid medium modeled assembly flow. To validate present model, simulations for a three-assembly model were performed with Realistic modeling, traditional porous medium model and hybrid medium model, respectively. The influences of Uniform/Non-Uniform power distribution among assemblies and the Peclet number within the assembly flow have been considered. Compared to traditional porous medium model, present model shows a better agreement with in Realistic modeling prediction of the temperature distribution and the radial heat transfer between the inter-wrapper flow and the assembly flow.
Korean Journal of Construction Engineering and Management
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v.16
no.5
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pp.114-122
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2015
Real estate development requires significant amount of capital investment. The project duration has been increased according to its enlarged size. For this reason, cost overrun and time delay are important risk factors that should be managed properly. As a method to hedge the risk, varoius real option methods have been presented. However, conventional project value assesment methods such as NPV(Net Present Value) have weakness to support decision making by reflecting dynamic situations in terms of variation of cost and time. Furthermore, the decision making process is serious of actions rather than discrete event. The purpose of this paper is to present a multiple real option valuation method to overcome the deterministic aspect of real option presented in previous research and practice. The method is developed as following: firstly, to select the model that can be applied in the real estate development project through a survey from previous literature on real options analysis; secondly, to apply data from office development case in order to verify the model by applying conventional real option and multiple real option valuation. According to analysis result, multiple real option provides enhanced values comparing to NPV and single real option.
The purpose of this study is to evaluate the phenomenon of attachment and spreading of the cultured rat calvarial cell inoculated on their surface of different kinds of biodegradable membrane which had been used on tissue regeneration on periodontal defects by using scanning electron microscope. In this experiment 30 Sprague-Dawley male rats (mean BW 150gm) were used to harvest abundant number of cell in the short period. The rats were sacrificed by decapitatioan to obtain the calvaria for bone cell culture. Calvarial cells were cultured with Dulbecco's Modified Essential Medium contained with 10% Fetal Bovine Serum under the conventional conditions. Biodegradable barrier membrane were collected with collagen type, and were divided into 3 different kind of surface such as scattered, polarized and fine-net type as their surface texture. Microcover plate which usually used for cell culture was used as control for smooth surface. All the membrane were seeded with cultured calvarial cell on their surface. The number of cell inoculated on the membrane were $1{\times}10^6$ Cells/ml. After the culture as designed time, all the membrane were washed with 0.1 M Phosphate Buffered saline and fuxed with 2.5% Glutaraldehyde. And all specimen were treated with $OsO_4$, and Tannic acid before drying the cell for coating the cell with gold. Scanning Electron Microscope was used to observation. The following results were obtained. I. During the whole period of experiment, the phenomenon of cell attachment and spreading were revealed similar pattern to compare with smooth surface culture plate and ordinary culture dish. 2. The shape of cell attachment and spreading on the surface of barrier membrane were observed no remarked difference pattern between smooth surface culture plate and ordinary culture dish. 3. The cytoplasmic process of cultured calvaria cell extent to the deep portion of barrier membrane like as their own proper shape. 4. There were no remarkable relationships between the degree of cultured cell spreading and surface structure of barrier membrane. 5. Slight starified layer of cultured calvaria cell were observed on the scattered type of resorbable membrane, Conclusively, this study thus suggest that cultured bone cell inoculated onto the biodegradable barrier membrane may have an important role of carrier for many cell which could be used as new tissue regeneration, and those tissue engeering technique may become an new method in the approach to the repair of bone defects.
The study starts from the critical thought on why most of female-headed single-parent families are under poverty regardless of their hard working. The study is to explore the lives of low-income single-parent families as working poor and to develop comprehensive policies for them out of poverty and other social vulnerability, using the concept of social exclusion which has been broadly introduced in the field of social science. Specifically, the study aims to analyse the process of social exclusion of low-income single-parent households, by comparing the differential lives in the economic, social, political aspects among male-headed dual-parent household, female-headed single-parent household, and poor female-headed lone-parent household who is under the public poverty line. The study was based on the secondary data analysis. A total of 3182 samples(the first 2508, the second 235, and the third 439) were included. Using SAS 6.12, frequency, chi-square, means, and ANOVA were utilized for the statistical analysis. Results show that single mothers, on the whole, are vulnerable population in terms of employment, housing stability and income. Also, low-income households of single mothers are more negatively affected by the IMF economic crisis and carry more pessimism on their future. In comparison with male-headed households, single mothers have fewer support from social insurance as well as family resources and higher burdens of additional caring need for the old, the disabled, and the chronically ill. These findings identify the reality in which female-headed households with dependent children are exposed to the overall social vulnerability and thus are easily trapped into the social disadvantaged. finally, the study suggests the development of comprehensive policies and counter-measurements for low-income female-headed families to be the primary member in our society.
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