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Effects of $Ca^{++}$ Inhibitors on Compaction of Mouse 8-cell Embryos (생쥐 8세포배의 Compaction에 미치는 칼슘 Inhihitor의 영향)

  • Kim, Hee-Sun;Bae, In-Ha
    • Clinical and Experimental Reproductive Medicine
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    • v.21 no.1
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    • pp.49-62
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    • 1994
  • In mammalian development, the embryo which is in the process of compaction, involves a progressive flattening of blastomeres against each other with the consequence that the embryo assumes a spherical shape. This stage happens in the first differentiation. The present study was aimed to examine the involvement of other metal ions in compaction by treating with various divalent cations in the absence of $Ca^{++}$. When 8-cell embryos were cultured in $Ca^{++}$-free medium for 24hrs, they developed to 16-cell stage but did not compaction, and degenerated after 48hrs of culture. Embryos were cultured in $Ca^{++}$-free medium for 24hrs and then transferred to the control medium showed the normal compaction afterwards. When 8-cell embryos were cultured in the presence of $Ni^{++}$, known as a $Ca^{++}$ inhibitor, they cleaved to 16-cell stage but did not compact in the absence of $Ca^{++}$. On the other hand, embryos cultured in the media containing both $Ca^{++}$ and $Ni^{++}$ developed normally so that they underwent compaction during culture for 48hrs. However, they failed to hatch during further 24hrs in the same medium, indicationg that $Ni^{++}$ may exert some harmful effects. Embryos grow in the control medium that contained $Ca^{++}$ but not $Ni^{++}$, developed to the hatched blastocysts. The treatment with $Cd^{++}$ $10^{-1}$,$10^{-2}{\mu}M$, $Mn^{++}$ or $Ba^{++}$ 10,100, $1000{\mu}M$ in $Ca^{++}$-free medium, respectively, inhibited compaction and embryonic degeneration began as in $Ca^{++}$-free medium. When 3, 5, 10mM of $Sr^{++}$, known as a substitute for $Ca^{++}$ in cell, was added to $Ca^{++}$-free medium, respectively, compaction was induced unlike the above metal ions. Embryos were cultured in $Sr^{++}$ developed to blastocysts, but failed to hatch after 72hrs and degenrated. On the other hand, when embryos were cultured in 3, 5, 10mM of $Sr^{++}$ but in $Ca^{++}$-free medium for 24hrs respectively and then transferred to the control, they showed the similiar development as that in the control.

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The Effect of Foliar Application to Improve Putting Green Performance (벤트그래스 그린 관리를 위한 엽면 시비의 효과)

  • Hong, Beom-Seok;Tae, Hyun-Sook;Oh, Sang-Hun;Cho, Yong-Sup
    • Asian Journal of Turfgrass Science
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    • v.25 no.1
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    • pp.94-99
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    • 2011
  • This study was performed to investigate the effect of foliar feeding as the fertilization on creeping benrgrass green. Research results have showed that the visual quality of bentgrass as well as shoot density and chlorophyll index dramatically improved after foliar feeding. Shoot density of bent grass in foliar based fertilization was 2.8 ea/$cm^2$ higher than that of the liquid based fertilization during the experiment period, moreover that the foliar feeding is more effective to alleviate shoot density during the rainy season in summer. Results showed that foliar feeding was key role to achieve the stable visual quality, chlorophyll index and maintained Normalized Difference Vegetation Index (NDVI) throughout the year in addition to get fast recovery after aeration and dry spot. These were consistent with the previous research of foliar feeding which directly provided nutrient to the turfgrass leaf thus increased turfgrass growth within a short time. It is projected to be a especially adequate management program during the hot summer season in which the nutrition feeding is low. However, root growth was no significant difference between foliar based fertilization and liquid based fertilization. Golf Course managers also should consider about the number of frequent fertilizing. Lastly, it was no big change in chemical property by soil foliar based fertilization which is meaning of preventing the soil salinization, meanwhile, it is prone to reach the nutrient deficiency. It hopes to be settled as the general fertilization in golf course of Korea, since there are many advantages of foliar based fertilization program. It is suggested to study more practical process of foliar feeding through the further research.

A Study on Development of Safety Management Evaluation Index for Shipping Company (해운선사 안전관리 평가지표 개발에 관한 연구)

  • Kim, Hwa-Young
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.19 no.6
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    • pp.629-636
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    • 2013
  • There is no safety evaluation index with marine accidents and information of ship's safety management result for good grasp of safety management level of shipping company and effective monitoring. In this paper, we proposed a SMEI(Safety Management Evaluation Index) for evaluation of shipping companies. The SMEI is consist of MAR(marine accidents ratio), SSMR(ship safety management ratio) and SAP(safety advantage point) through brainstorming of the expert group and AHP(analysis hierarchy process)in previous study. And weights of SMEI are calculated using experts's opinions through the Delphi method. We also verified the validity of proposed SMEI to 119 Korean shipping companies with 916 ocean-going ships of Korean flag, which grouped three part (small, mid and big) by number of ship and gross tonnage. And we found out 19 shipping companies, 8 companies in group1 & group2 respectively and 3 companies in group 3, with score below 1.0 by SMEI. In these companies, there are no marine accidents in recent 3 years and appeared low frequency remarkably in the detention and mojor non-conformity by data analysis.

Transient Structural Analysis of Piston and Connecting Rods of Reciprocating Air Compressor Using FEM (FEM을 이용한 왕복동 공기압축기의 피스톤 및 커넥팅로드의 구조해석)

  • Pham, Minh-Ngoc;Yang, Chang-Jo;Kim, Jun-Ho;Kim, Bu-Gi
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.23 no.4
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    • pp.393-399
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    • 2017
  • In a reciprocating compressor, the piston and connecting rod are important parts. Excess mechanical stress on these parts may cause damage, and broken parts are expensive and difficult to replace. Therefore, it is necessary to analyze the mechanical stress affecting durability and longevity. The main purpose of this study was to identify locations of maximum stress on pistons and connecting rods. Based on dynamic calculation of the working process of a specific air compressor, an analysis of piston and connecting rod performance has been completed. A three-dimensional model for the air compressor's pistons and connecting rods was built separately, and FEM analysis of these components was carried out using a numerical method. The pistons were loaded by pressure which was changed according to crankshaft angle without thermal boundary conditions. The simulation results were used to predict and estimate stress concentration as well as the value of this stress on pistons and connecting rods. The maximum equivalent stress calculated are over 190 MPa on pistons and 123 MPa on connecting rods at crank angle $135^{\circ}$ and $225^{\circ}$ but these are under tensile yield strength. Besides, the calculated safety factors of connecting rods and pistons is higher than 1. Moreover, the results obtained can be used to provide manufacturers with references to optimize the design of pistons and connecting rods for reciprocating compressors.

The Current Status of Domestic Marine Salvage Industry and Measures for its Promotion (국내 해양 구난산업의 현황과 육성방안에 관한 연구)

  • An, Kwang;Jeong, Jung-Sik;Kim, In-Chul;Kim, Deuk-Bong
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.26 no.2
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    • pp.149-155
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    • 2020
  • Since marine accidents cause large-scale damage to human lives and the marine environment, prompt response measures are crucial including salvage operations for ships and cargoes along with lifesaving operations. In Korea, marine salvage operations are mainly dependent on global salvage companies in case of large-scale marine accidents due to lack of national capacity for marine salvage. The purpose of this study was to suggest public measures for the promotion of the private salvage industry by identifying the current situation and problems in the domestic marine salvage industry. As a result of the study, the measures of setting up a dedicated agency to support the domestic salvage industry and the function of the dedicated agency were identified and presented. In addition, the main points of the bill of "Law on the Promotion of the Domestic Salvage Industry" were presented to support and promote the salvage industry. To prepare the draft bill of law, relevant domestic laws and international conventions were examined to define the terms and regulations. Based on the legislation enacted as a result of this study, relevant ministries should focus on the legislative process. Legislation can be pursued by competent ministries in cooperation with the private sector and academia. It is expected that this study will help in the development of the domestic marine salvage industry and enhance the national response capacity for marine accidents in Korea.

Collapse Analysis of Ultimate Strength Considering the Heat Affected Zone of an Aluminum Stiffened Plate in a Catamaran (카타마란 알루미늄 보강판의 열영향부 효과를 고려한 최종강도 붕괴 해석)

  • Kim, Sung-Jun;Seo, Kwang-Cheol;Park, Joo-Shin
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.26 no.5
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    • pp.542-550
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    • 2020
  • The use of high-strength aluminum alloys for ships and of shore structures has many benefits compared to carbon steels. Recently, high-strength aluminum alloys have been widely used in onshore and of shore industries, and they are widely used for the side shell structures of special-purpose ships. Their use in box girders of bridge structures and in the topside of fixed platforms is also becoming more widespread. Use of aluminum material can reduce fuel consumption by reducing the weight of the composite material through a weight composition ratio of 1/3 compared to carbon steel. The characteristics of the stress strain relationship of an aluminum structure are quite different from those of a steel structure, because of the influence of the welding[process heat affected zone (HAZ). The HAZ of aluminum is much wider than that of steel owing to its higher heat conductivity. In this study, by considering the HAZ generated by metal insert gas (MIG) welding, the buckling and final strength characteristics of an aluminum reinforcing plate against longitudinal compression loads were analyzed. MIG welding reduces both the buckling and ultimate strength, and the energy dissipation rate after initial yielding is high in the range of the HAZ being 15 mm, and then the difference is small when HAZ being 25 mm or more. Therefore, it is important to review and analyze the influence of the HAZ to estimate the structural behavior of the stiffened plate to which the aluminum alloy material is applied.

Prediction of ground-condition ahead of tunnel face using electromagnetic wave - analytical study (전자기파를 이용한 터널전방 예측 -해석기법 중심으로)

  • Choi, Jun-Su;Cho, Gye-Chun;Lee, Geun-Ha;Yoon, Ji-Nam
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.6 no.4
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    • pp.327-343
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    • 2004
  • During tunnel construction, ground failures often occur due to existence of weak zones, such as faults, joints, and cavities, ahead of tunnel face. It is hard to detect effectively weak zones, which can lead underground structure to fail after excavation and before supporting, by using conventional characterization methods. In this study, an enhanced analytical method of predicting weak zones ahead of tunnel face is developed to overcome some problems in the conventional geophysical exploration methods. The analytical method is based on Coulomb's and Gauss' laws with considering the characteristics of electric fields subjected to rock mass. Using the developed method, closed form solutions are obtained to detect a spherical shaped zone and an oriented fault ahead of tunnel face respectively. The analytical results suggest that the presence of weak zones and their sizes, location, and states can be accurately predicted by combining a proper inversion process with resistance measured from several electrodes on the tunnel face. It appears that the skin depth or resistivity in rock mass is affected by the diameter of tunnel face, natural electric potential and noises induced by experimental measurement and spatial distribution of uncertain properties. The developed analytical solution is verified through experimental tests. About 1800 concrete blocks of 5cm by 5cm by 5cm in size are prepared and used to model a joint rock mass around tunnel face. Weak zones are simulated ahead of tunnel face with a material which has relatively higher conductivity than concrete blocks. Experimental results on the model test show a good agreement with analytical results.

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Estimation of design parameters of TBM using punch penetration and Cerchar abrasiveness test (압입시험 및 세르샤 마모시험에 의한 TBM의 설계변수 추정)

  • Jeong, Ho-Young;Lee, Sudeuk;Jeon, Seokwon
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.16 no.2
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    • pp.237-248
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    • 2014
  • Linear cutting test is known to be very effective to determine machine parameters (i.e. thrust force and torque) and to estimate penetration rate of TBM and other operation conditions. Although the linear cutting test has significant advantages, the test is expensive and time-consuming because it requires large size specimen and high load capacity of the testing machine. Therefore, a few empirical prediction models (e.g. CSM, NTNU and QTBM) alternatively adopt laboratory index tests to estimate design parameters of TBM. This study discusses the estimation method of TBM machine parameters and disc cutter consumption using punch penetration test and Cerchar abrasion test of which the researches are rare. The cutter forces and cutter consumption can be estimated by the empirical models derived from the relationship between laboratory test result with field data and linear cutting test data. In addition, the estimation process was programmed through which the design parameters of TBM (e.g. thrust, torque, penetration rate, and cutter consumption) are automatically estimated using laboratory test results.

Development of a programming logic to estimate the wall friction coefficient in vehicle tunnels with piston effects (교통환기력이 작용하는 터널 내 벽면마찰계수 추정을 위한 프로그램 로직 개발)

  • Kim, Hyo-Gyu;Choi, Pan-Gyu;Ryu, Ji-Oh;Lee, Young-Hoon
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.20 no.1
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    • pp.39-53
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    • 2018
  • Generally, the total ventilation resistance coefficient in a tunnel consists of inlet/outlet loss coefficient, wall friction coefficient, and other loss coefficient caused by sudden expansion and contraction of cross-section, etc. For the tunnel before opening, when the running ventilation fan is stopped, the wind speed in the tunnel is reduced by the total ventilation resistance drag. The velocity decay method is comparatively stable and easy to estimate the wall friction coefficient in the pre-opening tunnel. However, the existing study reported that when the converging wind speed is a negative value after the ventilation fan stops, it is difficult to estimate the wall friction coefficient according to the velocity decay method. On the other hand, for the operating tunnel in which the piston effect acts, a more complex process is performed; however, a reasonable wall friction coefficient can be estimated. This paper aims at suggesting a method to minimize the measurement variables of the piston effect and reviewing a method that can be applied to the operating tunnel. Also, in this study, a new method has been developed, which enables to calculate an variation of the piston effect if the piston effect is constant with a sudden change of external natural wind occurring while the wind speed in the tunnel decreases after the ventilation fan stops, and a programming logic has been also developed, which enables dynamic simulation analysis in order to estimate the wall friction coefficient in a tunnel.

Fundamental study on enlargement method of existing subway tunnel during operation for sidetrack construction (부본선 건설을 위한 기존 지하철 터널의 운영 중 확폭 방안에 대한 기초연구)

  • Lee, Hyobum;Koh, Sung-Yil;Jun, Jonghun;Yoon, Hee Taek;Yi, Na Hyun;Choi, Hangseok
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.22 no.1
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    • pp.59-76
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    • 2020
  • As a continuous increase in demand for the transportation infrastructure in the metropolitan area, the renovation of existing metropolitan and urban railway lines for the rapid transport system requires the construction of sidetrack that can operate local and express trains simultaneously. However, the construction of sidetrack after stopping the operation of the existing subway line causes a lot of economic losses, therefore it is essential to study the tunnel enlargement scheme during the operation of the existing subway tunnel. Accordingly, in this paper, basic research on the enlargement plan of the existing subway tunnel was carried out for the renovation of the existing subway line. In order to investigate the method for the sidetrack construction, the Government Complex Gwacheon station on the Gwacheon line of subway line 4 was selected as a virtual research station. Subsequently, four construction plans including tunnel cross-sectional plan for each section were reviewed and constructability and economic feasibility were compared. Finally, the stability assessment was conducted for the selected construction plan which was considered to be relatively unstable by 3-D full numerical analysis considering the sidetrack construction process.