• BMB Reports
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• 제49권10호
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• pp.572-577
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• 2016

The short gastrulation (sog) shadow enhancer directs early and late sog expression in the neurogenic ectoderm and the ventral midline of the developing Drosophila embryo, respectively. Here, evidence is presented that the sog primary enhancer also has both activities, with the late enhancer activity dependent on the early activity. Computational analyses showed that the sog primary enhancer contains five Dorsal (Dl)-, four Zelda (Zld)-, three Bicoid (Bcd)-, and no Single-minded (Sim)-binding sites. In contrast to many ventral midline enhancers, the primary enhancer can direct lacZ expression in the ventral midline as well as in the neurogenic ectoderm without a canonical Simbinding site. Intriguingly, the impaired transcriptional synergy between Dl and either Zld or Bcd led to aberrant and abolished lacZ expression in the neurogenic ectoderm and in the ventral midline, respectively. These findings suggest that the two enhancer activities of the sog primary enhancer are functionally consolidated and geographically inseparable.

• BMB Reports
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• 제30권4호
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• pp.269-273
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• 1997

Heptocvte-specific expression induced by Hepatitis B virus (HBV) enhancer 2-core gene promoter was examined in various hepatocyte and non-hepatocyte cell lines. using non-viral and retroviral vector systems in which chloramphenicol acetyltransferase (CAT) is used as a reporter. The non-viral plasmid containing the HBV enhancer 2-core promoter exhibited 22 and 66% of CAT activities in hepatoma cell lines. HepG2 and Hep3B, respectively when compared with CAT activity expressed by CMV promoter. The CAT activities, however. were found to be marginal in other tested hepatoma cell lines as well as mouse primary hepatocytes and non-hepatocytes. The HBV enhancer 2 located upstream the CMV promoter did not affect the CMV promoter activity nor provided hepatocyte-specific expression. Transfection of retroviral plasmid DNA containing the HBV enhancer 2-core promoter as an internal promoter exhibited high and specific CAT expression in HepG2 and Hep3B cell lines but the activity value was 5 to 10 fold lower than the non-viral plasmid with identical promoter. These results suggest that the usage of HBV enhancer 2-core promoter for liver specific expression is limited to certain vectors and hepatocyte cell lines.

• Journal of Pharmaceutical Investigation
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• 제32권1호
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• pp.35-40
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• 2002

We prepared a novel dosage form, peel-off type soft hydrogel using poly(vinyl alcohol), and evaluated the effect of skin penetration enhancer on the indomethacin release from soft hydrogel by in vitro permeation and in vivo absorption test. In this study, we used four enhancers-urea, dimethyl urea, 1,1,3,3-tetramethyl urea, and pirotiodecane (1-[2(decylthio)ethyl]azacyclopentane-2-one, $HPE-101^{circledR}$). In addition, we evaluated the primary skin irritation test of soft hydrogel using rabbit. From these results, we could find the pirotiodecane was a prominent enhancer, and soft hydrogel seemed to be safe and have no irritancy.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 1996년도 추계학술대회 논문집
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• pp.267-271
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• 1996

In this study, performance improvement of the inverse modeling as the on-line control method for the estimation, control experiment is performed. As the modeling errors is occurred in duct system arbitrarily, a case using the filtered-x LMS algorithm only as the control method, a case using tile inverse modeling method only and a case using the inverse modeling with the adaptive line enhancer are compared. The estimation errors between real secondary path transfer functions and the estimated and the control performances of primary noises with these estimated transfer functions are compared.

• 대한기계학회논문집A
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• 제21권8호
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• pp.1332-1338
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• 1997

In the case of the transfer function for the secondary path is dependent on time, the on-line method which can model it is continuously must be applied to the active noise control technique. And the adaptive random noise technique among the on-line methods is effective in the narrow-band control. In this method, the signal to noise ratio between random noise for modeling and primary noise is low. Therefore, the estimations of transfer function will be prone to inaccuracies and the convergence time will be too long. Such imperfections will have an influence upon the performance of an active noise controller. In this study, t enhance the signal to noise ratio, the on-line method that is combined the conventional adaptive random noise technique and the adaptive line enhancer, is proposed. By using proposed on-line method, a rigorous system identification and control of primary noise have been implemented.

• 반도체공학회 논문지
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• 제2권1호
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• pp.17-20
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• 2024

본 논문은 전통적인 자원 집약적인 컴퓨터 비전 모델의 한계를 해결하기 위해 저전력 엣지 장치에 최적화된 새로운 경량 객체 검출 모델을 제안합니다. 제안된 검출기는 Single Shot Detector (SSD)에 기반하여 소형이면서도 견고한 네트워크를 설계하였고, 작은 객체를 효율적으로 감지하는 데 있어 효율성을 크게 향상시키도록 모델을 구성하였다. 이 모델은 주로 두 가지 구성요소로 구성되어 있습니다: Depthwise 와 Pointwise Convolution 레이어를 사용하여 효율적인 특징 추출을 위한 Light_Block, 그리고 작은 객체의 향상된 감지를 위한 Enhancer_Block 으로 나누었다. 우리의 모델은 300x480 의 이미지 크기를 가진 Udacity 주석이 달린 데이터셋에서 처음부터 훈련되었으며, 사전 훈련된 분류 가중치의 필요성을 제거하였다. 약 0.43M 의 파라미터로 5.5MB 만의 무게를 가진 우리의 검출기는 평균 정밀도 (mAP) 27.7%와 140 FPS 의 처리 속도를 달성하여, 정밀도와 효율성 모두에서 기존 모델을 능가하였다. 따라서, 본 논문은 추론의 정확성을 손상시키지 않으면서 엣지 장치를 위한 객체 검출에서의 효과적인 경량화를 보여주고 있다.

• 대한기계학회논문집A
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• 제21권11호
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• pp.1837-1843
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• 1997

This study experimentally demonstrates the use of active muffler attached to the automotive exhaust system to reduce exhaust noise. For improving the signal to noise ratio in the process of estimation of secondary path transfer functions, the on-line algorithm that conventional inverse modeling is combined with adaptive line enhancer is used as the control algorithm. Active muffler is designed that the primary noise and the control sound are propagated as a plane wave in the outlet. Therefore, the error microphone could be placed out of the tail pipe center of a high temperature and the radiation noise to the outside could be reduced in the whole area around the outlet. The control experiment for reducing exhaust noise with active muffler is implemented during run-up at no load. From the experimental results presented, compared with the conventional off-line method, the proposed on-line method is capable to acquire a reduction of exhaust noise above 5 dB in overall sound power level.

• 대한기계학회논문집A
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• 제21권2호
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• pp.281-287
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• 1997

In the conventional inverse modeling method for on-line modeling of the secondary path transfer function, the signal to noise ratio between the arbitrary random signal and the plant noise have to keep at -10 - -20 dB. For these reasons, the modeling can't be exactly implemented by the conventional method alone and the convergence time for modeling becomes too long. In this study, by combining the conventional inverse modeling method with an adaptive line enhancer, or with an adaptive noise canceller, a rigorous transfer functions of secondary path modeling and the control of a primary noise have been implemented simultaneously.

• Molecules and Cells
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• 제26권3호
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• pp.291-298
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• 2008

Human karyopherin ${\beta}3$, highly homologous to a yeast protein secretion enhancer (PSE1), has often been reported to be associated with a mediator of a nucleocytoplasmic transport pathway. Previously, we showed that karyopherin ${\beta}3$ complemented the PSE1 and KAP123 double mutant. Our research suggested that karyopherin beta has an evolutionary function similar to that of yeast PSE1 and/or KAP 123. In this study, we performed yeast two-hybrid screening to find a protein which would interact with karyopherin ${\beta}3$ and identified apolipoprotein A-I (apo A-I), a secretion protein with a primary function in cholesterol transport. By using in vitro binding assay, co-immunoprecipitation, and colocalization studies, we defined an interaction between karyopherin ${\beta}3$ and apo A-I. In addition, overexpression of karyopherin ${\beta}3$ significantly increased apo A-I secretion. These results suggest that karyopherin ${\beta}3$ plays a crucial role in apo A-I secretion. These findings may be relevant to the study of a novel function of karyopherin ${\beta}3$ and coronary artery diseases associated with apo A-I.

• 한국음향학회지
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• 제29권1호
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• pp.10-17
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• 2010

수중에서 소나를 이용하여 표적을 탐지하고자 할 때 자함에서 발생되는 소음을 감소시켜 표적의 탐지성능을 향상시키는 것은 매우 중요한 일이다. 본 논문에서는 수상함에서 선체고정형소나(HMS)를 사용하는 경우 두 가지 운영모드에 대하여 자함의 소음감소 성능을 분석하였다. 운영자모드에서는 ALE(Adaptive Line Enhancer)기법을 적용하여 자함신호의 광대역 성분을 감소하므로 토널 성분의 탐지성능을 향상하였으며, 자동모드에서는 주 입력신호와 상관관계를 가진 기준 입력신호를 선정하여 조향 방위로 유입되는 잡음신호를 제거하였다. 적응필터를 설계하기 위하여 NLMS(Normalized LMS)알고리즘을 이용하였으며 실제 해상실험 데이터를 이용하여 시뮬레이션을 수행하므로 제안한 기법의 성능을 확인하였다.