• BMB Reports
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• v.49 no.10
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• pp.572-577
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• 2016

The short gastrulation (sog) shadow enhancer directs early and late sog expression in the neurogenic ectoderm and the ventral midline of the developing Drosophila embryo, respectively. Here, evidence is presented that the sog primary enhancer also has both activities, with the late enhancer activity dependent on the early activity. Computational analyses showed that the sog primary enhancer contains five Dorsal (Dl)-, four Zelda (Zld)-, three Bicoid (Bcd)-, and no Single-minded (Sim)-binding sites. In contrast to many ventral midline enhancers, the primary enhancer can direct lacZ expression in the ventral midline as well as in the neurogenic ectoderm without a canonical Simbinding site. Intriguingly, the impaired transcriptional synergy between Dl and either Zld or Bcd led to aberrant and abolished lacZ expression in the neurogenic ectoderm and in the ventral midline, respectively. These findings suggest that the two enhancer activities of the sog primary enhancer are functionally consolidated and geographically inseparable.

• BMB Reports
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• v.30 no.4
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• pp.269-273
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• 1997

Heptocvte-specific expression induced by Hepatitis B virus (HBV) enhancer 2-core gene promoter was examined in various hepatocyte and non-hepatocyte cell lines. using non-viral and retroviral vector systems in which chloramphenicol acetyltransferase (CAT) is used as a reporter. The non-viral plasmid containing the HBV enhancer 2-core promoter exhibited 22 and 66% of CAT activities in hepatoma cell lines. HepG2 and Hep3B, respectively when compared with CAT activity expressed by CMV promoter. The CAT activities, however. were found to be marginal in other tested hepatoma cell lines as well as mouse primary hepatocytes and non-hepatocytes. The HBV enhancer 2 located upstream the CMV promoter did not affect the CMV promoter activity nor provided hepatocyte-specific expression. Transfection of retroviral plasmid DNA containing the HBV enhancer 2-core promoter as an internal promoter exhibited high and specific CAT expression in HepG2 and Hep3B cell lines but the activity value was 5 to 10 fold lower than the non-viral plasmid with identical promoter. These results suggest that the usage of HBV enhancer 2-core promoter for liver specific expression is limited to certain vectors and hepatocyte cell lines.

• Journal of Pharmaceutical Investigation
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• v.32 no.1
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• pp.35-40
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• 2002

We prepared a novel dosage form, peel-off type soft hydrogel using poly(vinyl alcohol), and evaluated the effect of skin penetration enhancer on the indomethacin release from soft hydrogel by in vitro permeation and in vivo absorption test. In this study, we used four enhancers-urea, dimethyl urea, 1,1,3,3-tetramethyl urea, and pirotiodecane (1-[2(decylthio)ethyl]azacyclopentane-2-one, $HPE-101^{circledR}$). In addition, we evaluated the primary skin irritation test of soft hydrogel using rabbit. From these results, we could find the pirotiodecane was a prominent enhancer, and soft hydrogel seemed to be safe and have no irritancy.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1996.11a
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• pp.267-271
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• 1996

In this study, performance improvement of the inverse modeling as the on-line control method for the estimation, control experiment is performed. As the modeling errors is occurred in duct system arbitrarily, a case using the filtered-x LMS algorithm only as the control method, a case using tile inverse modeling method only and a case using the inverse modeling with the adaptive line enhancer are compared. The estimation errors between real secondary path transfer functions and the estimated and the control performances of primary noises with these estimated transfer functions are compared.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.21 no.8
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• pp.1332-1338
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• 1997

In the case of the transfer function for the secondary path is dependent on time, the on-line method which can model it is continuously must be applied to the active noise control technique. And the adaptive random noise technique among the on-line methods is effective in the narrow-band control. In this method, the signal to noise ratio between random noise for modeling and primary noise is low. Therefore, the estimations of transfer function will be prone to inaccuracies and the convergence time will be too long. Such imperfections will have an influence upon the performance of an active noise controller. In this study, t enhance the signal to noise ratio, the on-line method that is combined the conventional adaptive random noise technique and the adaptive line enhancer, is proposed. By using proposed on-line method, a rigorous system identification and control of primary noise have been implemented.

• Transactions on Semiconductor Engineering
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• v.2 no.1
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• pp.17-20
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• 2024

This paper presents a novel lightweight object detection model tailored for low-powered edge devices, addressing the limitations of traditional resource-intensive computer vision models. Our proposed detector, inspired by the Single Shot Detector (SSD), employs a compact yet robust network design. Crucially, it integrates an 'enhancer block' that significantly boosts its efficiency in detecting smaller objects. The model comprises two primary components: the Light_Block for efficient feature extraction using Depth-wise and Pointwise Convolution layers, and the Enhancer_Block for enhanced detection of tiny objects. Trained from scratch on the Udacity Annotated Dataset with image dimensions of 300x480, our model eschews the need for pre-trained classification weights. Weighing only 5.5MB with approximately 0.43M parameters, our detector achieved a mean average precision (mAP) of 27.7% and processed at 140 FPS, outperforming conventional models in both precision and efficiency. This research underscores the potential of lightweight designs in advancing object detection for edge devices without compromising accuracy.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.21 no.11
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• pp.1837-1843
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• 1997

This study experimentally demonstrates the use of active muffler attached to the automotive exhaust system to reduce exhaust noise. For improving the signal to noise ratio in the process of estimation of secondary path transfer functions, the on-line algorithm that conventional inverse modeling is combined with adaptive line enhancer is used as the control algorithm. Active muffler is designed that the primary noise and the control sound are propagated as a plane wave in the outlet. Therefore, the error microphone could be placed out of the tail pipe center of a high temperature and the radiation noise to the outside could be reduced in the whole area around the outlet. The control experiment for reducing exhaust noise with active muffler is implemented during run-up at no load. From the experimental results presented, compared with the conventional off-line method, the proposed on-line method is capable to acquire a reduction of exhaust noise above 5 dB in overall sound power level.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.21 no.2
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• pp.281-287
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• 1997

In the conventional inverse modeling method for on-line modeling of the secondary path transfer function, the signal to noise ratio between the arbitrary random signal and the plant noise have to keep at -10 - -20 dB. For these reasons, the modeling can't be exactly implemented by the conventional method alone and the convergence time for modeling becomes too long. In this study, by combining the conventional inverse modeling method with an adaptive line enhancer, or with an adaptive noise canceller, a rigorous transfer functions of secondary path modeling and the control of a primary noise have been implemented simultaneously.

• Molecules and Cells
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• v.26 no.3
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• pp.291-298
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• 2008

Human karyopherin ${\beta}3$, highly homologous to a yeast protein secretion enhancer (PSE1), has often been reported to be associated with a mediator of a nucleocytoplasmic transport pathway. Previously, we showed that karyopherin ${\beta}3$ complemented the PSE1 and KAP123 double mutant. Our research suggested that karyopherin beta has an evolutionary function similar to that of yeast PSE1 and/or KAP 123. In this study, we performed yeast two-hybrid screening to find a protein which would interact with karyopherin ${\beta}3$ and identified apolipoprotein A-I (apo A-I), a secretion protein with a primary function in cholesterol transport. By using in vitro binding assay, co-immunoprecipitation, and colocalization studies, we defined an interaction between karyopherin ${\beta}3$ and apo A-I. In addition, overexpression of karyopherin ${\beta}3$ significantly increased apo A-I secretion. These results suggest that karyopherin ${\beta}3$ plays a crucial role in apo A-I secretion. These findings may be relevant to the study of a novel function of karyopherin ${\beta}3$ and coronary artery diseases associated with apo A-I.

• The Journal of the Acoustical Society of Korea
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• v.29 no.1
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• pp.10-17
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• 2010

In a passive sonar, the improvement of detection performance by using noise cancellation is usually a important problem. In this paper, we have analyzed the own-ship noise cancellation in the two operation modes which are used in the HMS system. In the operator mode, an adaptive line enhancer(ALE) is applied to improve the tonal detection by using broadband noise cancellation and the normalized least mean square(NLMS) algorithm is applied to the design of an adaptive filter. The reference input that is correlated with a primary input can be used to remove the noise incident on the observation directionin the automatic mode. Computer simulations with real sea that data show that the proposed adaptive noise canceller has good performance in passive detection under HMS operation.