• Journal of Pharmaceutical Investigation
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• 제36권6호
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• pp.363-369
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• 2006

Granisetron is a selective 5-HT3 receptor antagonist that is used therapeutically for the prevention of vomiting and nausea associated with emetogenic cancer chemotherapy. Although this drug is commercially available for intravenous and oral dosage, there is a need for intranasal delivery formulations in specific patient populations in which the use of these dosage forms may be unfeasible and/or inconvenient. A rapid and specific high-performance liquid chromatography method with mass spectrometric detection(LC-MS) was developed and validated for the analysis of granisetron in plasma after nasal administration in rats. This method has been validated for concentrations ranging from 5 to 1000 ng/ml with simple treatment. This technique has high level reproducibility, accuracy, and sensitivity. The method described was found to be suitable for the analysis of all samples collected during preclinical pharmacokinetic investigations of granisetron in rats after nasal administration. This study was aimed to investigate the feasibility of nasal delivery of granisetron for the elimination of vomiting. The effects of osmolarity, dosage volume at the same dose and applied dose on the nasal absorption of granisetron in rats were observed. No significant difference in the effect of osmolarity and dosage volume at the same dose was observed. As the applied dose of granisetron in nasal formulation increased, the absorption increased linearly. Based on these results it appears that only the applied dose(drug mass) determines the nasal absorption of granisetron. The bioavailability of granisetron on nasal administration of 4 mg/kg appeared to be comparable to that of intravenous administration of the same dose. These results suggest that granisetron can be efficiently delivered nasally and the development of nasal formulation will be feasible.

• Journal of Nutrition and Health
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• 제49권2호
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• pp.72-79
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• 2016

Purpose: The initiation of mandatory folic acid fortification using pteroylmonoglutamic acid (PteGlu) has reduced the rate of congenital malformations. However, it also appears to be responsible for several adverse effects, including increased cancer incidence. This may be related to physicho-chemical characteristics of PteGlu. This study examines the potential effect of high concentrations of PteGlu on a population subjected to mandatory folic acid fortification using an in vitro model. Methods: Caco-2 (colorectal cancer) and MCF7 (breast cancer) cell lines were cultured at 6 different PteGlu concentrations (0, 0.1, 1, 50, 250, and $500{\mu}g/ml$) for 6 days. Cell growth was determined using thiazolyl blue tetrazolium bromide assay. The genotype of dihydrofolate reductase 19bp deletion/insertion (DHFR 19-del) was also scored in cell lines using a restriction fragment length polymorphism technique to examine whether genetic variations may factor in cell proliferation. Results: PteGlu exhibited differential growth promoting properties between cell lines. Caco-2 cells did not show a significant growth difference at low concentrations compared to control, however, at higher concentrations, the growth showed a contrasting trend in the early experimental period, while MCF7 showed enhanced cell growth at all concentrations. The DHFR 19-del genotype differed in the two cell lines. Conclusions: Altered response to PteGlu by Caco-2 and MCF7 may reflect a tissue specific disease aetiology or genotype specific differential enzyme activity, for example by DHFR, to critical levels of PteGlu. As folic acid fortification is a blanket intervention, and DHFR and other enzyme activities vary between individuals, PteGlu intake may have an as yet undefined effect on health. These findings may be relevant when considering mandatory folic acid fortification for disease prevention.

• 한국농공학회논문집
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• 제47권3호
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• pp.59-68
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• 2005

The rainfall-runoff potential of Jangseong reservoir watershed was studied based on SCS (Soil Conservation Service, which is now the NRCS, Natural Resources Conservation Service, USDA) runoff curve number (CN) technique. Precipitation and reservoir operation data had been collected. The rainfall-runoff pairs from the watershed for ten years was estimated using reservoir water balance analysis using reservoir operation records. The maximum retention, S, for each storm event from rainfall-runoff pair was estimated for selected storm events. The estimated S values were arranged in descending order, then its probability distribution was determined as log-normal distribution, and associated CNs were found about probability levels of Pr=0.1, 0.5, and 0.9, respectively. A subwatershed that has the similar portions of land use categories to the whole watershed of Jangseong reservoir was selected and hydrologic monitoring was conducted. CNs for subwatershed were determined using observed data. CNs determined from observed rainfall-runoff data and reservoir water balance analysis were compared to the suggested CNs by the method of SCS-NEH4. The $CN_{II}$ measured and estimated from water balance analysis in this study were 78.0 and 78.1, respectively. However, the $CN_{II}$, which was determined based on hydrologic soil group, land use, was 67.2 indicating that actual runoff potential of Jangseong reservoir watershed is higher than that evaluated by SCS-NEH4 method. The results showed that watershed runoff potential for large scale agricultural reservoirs needs to be examined for efficient management of water resources and flood prevention.

• 한국관개배수논문집
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• 제18권2호
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• pp.87-100
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• 2011

This study is to delineate the watershed hydrological parameters such as area, slope, rain gauge weight, NRCS-CN and time of concentration (Tc) by using the Geographic Information Sytem (GIS) technique, and estimation of design flood for an ungauged watershed. Especially, we attempted to determine the Tc of ungauged watershed and develop simple program using the cell-based algorithm to calculates upstream or downstream flow time along a flow path for each cell. For a $19km^2$ watershed of tributary of Nakdong river (Seupmoon), the parameters including flow direction, flow accumulation, watershed boundary, stream network and Tc map were extracted from 30m Agreeburn DEM (Digital Elevation Model) and landcover map. And NRCS-CN was extracted from 30m landcover map and soil map. Design rainfall estimation for two rainfall gauge which are Sunsan and Jangcheon using FARD2006 that developed by National Institute for Disaster Prevention (NIDP). Using the parameters as input data of HEC-l model, the design flood was estimated by applying Clark unit hydrograph method. The results showed that the design flood of 50 year frequency of this study was $8m^3/sec$ less than that of the previous fundamental plan in 1994. The value difference came from the different application of watershed parameter, different rainfall distribution (Huff quartile vs. Mononobe) and critical durations. We could infer that the GIS-based parameter preparation is more reasonable than the previous hand-made extraction of watershed parameters.

• 대한교통학회지
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• 제29권3호
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• pp.73-82
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• 2011

최근 교통상황을 정확하게 관측할 수 있는 교통류 검지에 관한 기술개발과 더불어 개별차량 주행궤적을 이용한 교통안전도 평가기법에 대한 관심이 높아지고 있다. 본 연구에서는 개별차량의 주행궤적을 이용하여 다음시점(t+1) 의 후미추돌 확률을 산출하는 방법론을 제시하였다. 신뢰성 있는 예측 기법인 칼만 필터링(Kalman Filtering)을 이용하여 주행궤적을 예측하고, 예측된 시점에 대한 개별차량의 후미추돌 확률을 산출하였다. 안전도를 평가하는 확률모형을 수립하기 위해서 서해안 고속도로의 동영상 자료로부터 개별차량의 주행궤적을 추출하였다. 추출한 개별차량의 주행궤적 자료를 이항 로지스틱 회귀분석(Binary logistic regression)을 이용하여 차량의 차로변경 결정 확률 모형을 생성하였고, exponential decay function을 이용하여 surrogate safety measure(SSM)의 하나인 time-to-collision(TTC)기반의 추돌확률 모형을 생성하였다. 미시적 교통류 시뮬레이터인 VISSIM에서 추출한 개별차량의 주행궤적 데이터를 이용하여 제안된 방법론을 평가하였다. 본 연구의 결과는 교통류 감시, 제어 및 정보 시스템에 효과적으로 적용될 수 있으며, 나아가 교통사고 예방에 효율적인 대안이 될 수 있을 것으로 판단된다.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2008년도 춘계 학술발표회 초청강연 및 논문집
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• pp.938-946
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• 2008

This study is on sealing leakage holes where are in landfills to make clay cakes with clay particles, which have a negative surface charge using the method of electrophoresis. Generally, electrophoresis is the motion of charged particles in a colloid under the influence of an electric field; particles with a positive charge go to the cathode and negative to the anode. In this study in order to develop the prevention system of leakages of the leachate in landfills, one-dimensional electrophoresis tests were conducted for determining the properties of the motion of the electrophoresis and cutoff using the method of electrophoresis depending on various the effect factors such as types of clays, concentrations of the clays, and applied electric field. In case of the experiments of determining the optimum clays, Na and Ca-Bentonite, Na and Ca-Montmorillonite, which have greater zeta-potential, cation, exchange capacity as well as ability of cutoff, and Micro-cement inducing cementation were chosen and then the effect of those clays was investigated. Moreover, the properties of the motion and settling of the clays were investigated following electric field varied from 0 to 1V/cm at different concentration of the clays in order to determine both the properties of the motion of the clays and the efficiency of electric field when applying different direct current. Ultimately, the ability of cutoff was examined through measuring the permeability of the clay cakes derived from the one-dimensional electrophoresis tests.

• 정보처리학회논문지C
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• 제11C권5호
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• pp.697-702
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• 2004

디지털콘텐트는 복제가 용이하고 원본과 복사본이 동일하다는 특성 때문에 불법적인 복제와 유통의 방지를 통한 저작권의 보호에 어려움이 있다. 근래에는 웹을 기반으로 한 각종 디지털콘텐트 서비스 시스템이 상용화되고 있으며, 이것이 안정된 수익 모델로서 발전하기 위하여 적절한 저작권 보호 기술이 요구된다. 일반적으로 웹 기반의 저작권 보호를 위해서는 디지털콘텐트의 암호화를 통한 안전한 전송 방법을 사용한다. 이때 암호화된 디지털콘텐트의 크기는 증가하여 실행과정에 필요한 시간을 증가시킨다. 따라서 실행시간과 안전성을 고려한 시스템의 설계가 필요하다. 본 연구에서는, 디지털콘텐트의 저작권 관리 기술을 기반으로 부분 암호화를 통해 수행시간과 안전성을 고려한 디지털콘텐트 전송시스템을 설계하였다. 또한 분석을 통해 제안시스템의 성능을 평가하였다.

• KIEAE Journal
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• 제3권1호
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• pp.13-20
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• 2003

It is highly recognized that there is need for protection against indoor air pollution, as we realize environmental pollution is growing, For example, in an indoor environment, a person spends more than 80 percent of their time inside the building. Thus, concern about indoor decoration materials is growing, since they cause pollution in the rooms of an apartment, as well as in offices. As the indoor decoration materials become more diverse and lusurious, so the effect of VOCs(Volatile Organic Compounds) and HCHO(Formaldehy) is growing. The indoor decoration materials cause the Sick Building Syndrome, such as headaches, dizziness, or lack of concentraion, and they in turn cause serious deterioration in people's health. In this study, I probed the status of the indoor air pollution and carried on an investigation and analysis about the prevention technique. In doing so, I performed experimental tests and an assessment of the indoor decoration materials of an apartment. I also examined elements of the emitted and the emission. Finally, I examined the character of emissions, by changing environmental conditions, such as the temperature, humidity, and ventilation. With respect to VOCs tests, I applied the method of solid state adsorption using the adsorptive tube, based on the measurement of the American EPA TO-17, ASTM 5116-97, and the measurement of the Japanese Wall Decoration Industrial Association. The tested sample was analyzed by High Performance Liquid Chromatography, after going through the process of dissolvent extraction. As subjects of the test, Paint were selected. The process of this test is as follows; first, I figured out the character of the emission, by measuring the emitted concentration of VOCs and HOHC from the indoor decoration materials of an apartment. Second, I made a small-scale chamber and the test was processed in the chamber in order to suggest an environment-friendly prediction modlel development.

• 대한두개안면성형외과학회지
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• 제19권2호
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• pp.94-101
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• 2018

Background: Atrophy of muscle and fat often contributes to temporal hollowing after pterional craniotomy. However, the main cause is from the bony defect. Several methods to prevent temporal hollowing have been introduced, all with specific limitations. Autologous bone grafts are most ideal for cranial defect reconstruction. The authors investigated the effectiveness of bony defect coverage and temporal augmentation using pterional craniotomy bone flap. Methods: This study was conducted in 100 patients who underwent brain tumor excision through pterional approach from 2015 to 2016. Group 1 underwent pterional craniotomy with temporal augmentation and group 2 without temporal augmentation. In group 1, after splitting the calvarial bone at the diploic space, the inner table was used for covering the bone defect and as an onlay graft for temporal augmentation. The outcome is evaluated by computed tomography at 1-year follow-up. Results: The mean operative time for temporal augmentation was 45 minutes. The mean follow-up was 12 months. The ratio of temporal thickness of operated side to non-operated side was 0.99 in group 1 and 0.44 in group 2, which was statistically different. The mean visual analogue scale score was 1.77 in group 1 and 6.85 in group 2. Conclusion: This study demonstrated a surgical technique using autologous bone graft for successfully preventing the temporal hollowing and improved patient satisfaction.

• Parasites, Hosts and Diseases
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• 제51권2호
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• pp.237-241
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• 2013

Giardia lamblia is recognized as one of the most prevalent parasites in dogs. The present study aimed to establish a loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of G. lamblia from dogs. The fecal samples were collected and prepared for microscopic analysis, and then the genomic DNA was extracted directly from purified cysts. The concentration of DNA samples of G. lamblia were diluted by 10-fold serially ranging from $10^{-1}$ to $10^{-5}ng/{\mu}l$ for LAMP and PCR assays. The LAMP assay allows the amplification to be finished within 60 min under isothermal conditions of $63^{\circ}C$ by employing 6 oligonucleotide primers designed based on G. lamblia elongation factor 1 alpha ($EF1{\alpha}$) gene sequence. Our tests showed that the specific amplification products were obtained only with G. lamblia, while no amplification products were detected with DNA of other related protozoans. Sensitivity evaluation indicated that the LAMP assay was sensitive 10 times more than PCR. It is concluded that LAMP is a rapid, highly sensitive and specific DNA amplification technique for detection of G. lamblia, which has implications for effective control and prevention of giardiasis.