• Asian-Australasian Journal of Animal Sciences
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• v.12 no.1
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• pp.9-14
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• 1999

Factors affecting gene gun-mediated expression of the human erythropoietin gene were investigated in primary cultured oviduct cells from laying hens. The human erythropoietin gene was transfected by a gene gun method at $1.25{\mu}g$ per dish, and cultured in a synthetic serum-free medium for 72 hrs. The concentration of human erythropoietin mRNA was determined by RNA : RNA solution hybridization. In experiment 1, the effect of changing the shooting pressure of DNA-coated microparticles with nitrogen gas was tested at 20 and $60kgf/cm^2$. The results showed that the erythropoietin mRNA concentration was significantly higher at 60 than $20kgf/cm^2$. In experiment 2, the effects of supplementing the medium with fetal calf serum at 10%, and raising the shooting pressure from 60 to $80kgf/cm^2$ on the cell number and erythropoietin gene expression were examined. Although supplementation with fetal calf serum significantly increased the cell numbes compared with no supplemented controls (p < 0.05), erythropoietin mRNA concentration per $10^3$ cells was not affected. Raising the shooting pressure from 60 to $80kgf/cm^2$ did not affect either of the parameters, In experiment 3, the effect of supplementing ascorbate 2-phosphate at 0.5 mM was tested. The results indicated that the ascorbate supplementation significantly increased the cell number (p < 0.05), and tended to increase erythropoietin mRNA concentration (p < 0.1). Thus, for human erythropoietin gene expression by using the gene gun method, shooting pressure with nitrogen gas should be sufficient at $60kgf/cm^2$ and supplementation with ascorbate phosphate would be useful to enhance not only the cell proliferation but also erythropoietin gene expression.

• The Journal of the Acoustical Society of Korea
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• v.36 no.3
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• pp.165-171
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• 2017

The effect of the concentric solid tube inserted inside the vibrator on the sound field distribution was analyzed for the sound waves focused on the center axis in the fluid - filled cylindrical piezoelectric transducer. The sound waves radiated from the inside of the cylindrical piezoelectric vibrator are transmitted through the fluid medium and are reflected or transmitted on the wall surface of the solid tube, and are focused on the central axis. At this time, the sound field distribution centered on the acoustic tube varies depending on the acoustic impedance and the thickness of the solid tube. In order to theoretically analyze this, the transfer matrix for each medium is derived, and the sound pressure level at the center axis is theoretically analyzed. For the acrylic tube with various thicknesses, the changing trend in the sound pressure level measured on the central axis agrees well with the result of the theoretical analysis, and it confirmed that the sound pressure formed at the center changes very sensitively with the thickness of the solid tube.

• Microbiology and Biotechnology Letters
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• v.27 no.2
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• pp.91-95
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• 1999

To investigate the effect of salts on the production of erythritol by Torula sp., cells were grown on the media containing various concentrations of KCl or NaCl. Cell growth and glucose consumption rates decreased when KCl or NaCl concentration increased from 0.0 to 0.5M. The production of erythritol, however, was maximal at 0.3M aCl. The erythritol concentration of 54.3g/l in the medium containing 0.3M NaCl and 200g/l glucose was obtained after 120h. The production of erythritol decreased in cultures above 0.3M NaCl or 0.4M KCl due to the inhibition of cell growth. To elucidate the effect of salts more quantitatively, KCl and NaCl concentrations were converted to osmotic pressure. As the osmotic pressure increased, the yield of erythritol from glucose increased regardless of the kinds of salts and the yield of erythritol was approximately 49% at the osmolality of 2.4Os/kg. When the osmotic pressure increased to 2.5Os/kg, the specific growth rate of cells decreased but the production rate of erythritol increased. For the effective production of erythritol, osmotic pressure must be adjusted not to inhibit markedly the growth rate of cells and to stimulate the production rate of erythritol by supplementing salt.

• International Journal of Naval Architecture and Ocean Engineering
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• v.10 no.6
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• pp.711-729
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• 2018

This paper reports on the experimental investigations on the failure modes of ring-stiffened cylinder models subjected to external hydrostatic pressure. Nine models were welded from general structural steel. The shells were initially formed by cold-rolling, and flat-bar ring frames were welded to the shell. The hydrostatic pressure tests were conducted by using water as the medium in pressure chambers. The details of the preparation and main test were briefly explained. The investigation identified the consequence of the structural failure modes, including: shell yielding, local shell buckling between ring stiffeners, overall buckling of the shell together with the stiffeners, and interactive buckling mode combining local and overall buckling. In addition, the ultimate strengths were predicted by using existing design codes. Non-linear numerical computations were also conducted by employing the actual imperfection coordinates. Finally, accuracy and reliability of the predictions of design formulae and numerical were substantiated with the test results.

• PNF and Movement
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• v.16 no.3
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• pp.425-432
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• 2018

Purpose: The purpose of this study was to evaluate the relationship between foot pressure and upper arm PNF exercise conducted with elastic bands while standing. Methods: Eighteen asymptomatic patients consented to participate in the study. Foot pressure was measured in the standing position using the Gaitview system for proprioceptive neuromuscular facilitation stretching (PNF) using a right upper arm pattern. Resistance strength was varied based on the type of elastic band used (red=medium, green=heavy, and blue=extra heavy). Statistical significance of the results was evaluated using a repeated one-way ANOVA, and the Bonferroni method was used for the ad hoc test (SPSS ver. 18. for Windows). Results: A significant difference was observed in fore-foot, rear-foot, and everage pressure after upper arm PNF exercise. However, there was no significant difference according to the type of elastic band. Conclusion: Based on the results of this study, an upper arm PNF exercise with and without resistance may affect foot pressure in the standing position.

• The Bulletin of The Korean Astronomical Society
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• v.44 no.1
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• pp.77.1-77.1
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• 2019

It has been proposed by Gunn & Gott (1972) that galaxies may lose their interstellar gas by ram pressure due to the dense intra-cluster medium while falling to the cluster potential. The observational evidence for this process, which is known as ram pressure stripping, is increasing, and it is believed to be one of the key environmental effects that can dramatically change the star formation activity of galaxies and hence their evolution. Intriguingly however, some cases with clear signs of ram pressure stripping are found in the environment which betrays our expectations (e.g. large clustercentric distances), and our understandings to the detailed working principle behind ram pressure stripping seem to be still lacking. As one of the ways to gain more theoretical insights into the conditions for ram pressure stripping process, we have been comparing the gas truncation radius which is predicted based on the simple Gunn & Gott's prescription with what is actually observed in a sample of carefully selected Virgo galaxies. In this work, we present the results of our comparisons between the theoretically predicted truncation radius and the observationally measured truncation radius for individual galaxies in the sample and discuss which additional conditions are needed in order to fully understand the observations.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2004.05a
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• pp.408-413
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• 2004

High speed planning boats also have been required more and more the rational strength analysis and evaluation for the optimal structural design in respect of the structural lightness according to the high speed trend. Even though the suggestion of the simple type equation for the equivalent dynamic pressure is reasonable to design the scantling of ship structure conveniently, many research activities for more reasonable improvement of the simple design pressure, have been continued to suggest the more accurate equivalent static description of tire structural response such as the deflection and stress of hull structure. In this research, we focus on the aluminum bottom stiffened plate structure in which structural scantling is mainly depend on the local loads such as dynamic or impact pressure without other load effects and structural response for the simple dynamic equivalent pressure was investigated through the structural analysis. In order to investigate the structural response of the bottom stiffened plate structure subjected to the dynamic equivalent design pressure, linear and nonlinear structural analysis of the bottom stiffened plate structure of 4.3 ton aluminum planning boat was performed based on the equivalent static applied loads which were derived from the KR regulation and representative one among various dynamic equivalent pressure equations. From above analysis results, we found that the response such as deflection and stress of plate member was similar with the response results of one plate member model with fixed boundary, which was published previous paper and in case of KR design loading, all response of stiffened plate structure were within elastic limit. Through the nonlinear analysis, nearly elastic behavior including the slight geometrical nonlinear response was dominant but plastic local zone was appeared at $85\%$ limit load. Therefore, we can say that through tire linear and nonlinear analysis, this stiffened plate member has no structural strength problem based on the yield criteria in case within $60\%$ limit load except the other strength point of view such as the fatigue and buckling problem.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2018.11a
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• pp.404-405
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• 2018

• Journal of Mushroom
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• v.9 no.2
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• pp.74-79
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• 2011

This study was carried out to develop a method related to measuring the temperature of sterile medium in bottle cultivation. When the medium is sterilized, the device is able to be inserted inside of the medium and the temperature can be directly measured in real time although high temperature and pressure are detected in the sterilization. This device can be able to measure the sterilization temperature in short intervals inside of autoclave and medium used in bottle cultivation. As the method were applied to the field cultivation of mushroom, we could produce mushroom in consistent manner through the optimum sterilization of the medium.

• Explosives and Blasting
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• v.36 no.2
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• pp.1-9
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• 2018

In this paper, AUTODYN blasting simulation of single blast hole were conducted to evaluate the blasting effects of Polymer Gel. The coupling mediums used as the filling material around an explosive charge were air and gelatin. each simulation case was D I(decoupling index) 1.0, 1.25, 1.56 with air or polymer gel coupling materials. In order to evaluate blast effects full charge model was used as a reference for evaluation of blasting effects. The results of numerical analysis showed that fragmentation of a limestone model of were much more fractured by polymer gel medium than by air medium. As expected, the transmitted peak pressure was higher polymer gel coupled model than in air medium.