• Title/Summary/Keyword: Preservation science

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Effects of Storage Time on Bacteria Concentration and Sperm Parameters in Boar Semen (돼지 액상정액 보존 일수에 따른 정액내 세균과 정자 기능의 변화)

  • Chung, Ki-Hwa;Kim, In-Cheol
    • Reproductive and Developmental Biology
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    • v.36 no.3
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    • pp.163-166
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    • 2012
  • This study was conducted to determine the relationship between elapsed time after semen preservation on the changes of bacteria and semen quality. Semen was diluted with BTS(Beltsville Thawing Solution) extender without antibiotic for 7 days and sperm parameter and fertility were measured. Sperm motility was measured by CASA and total bacteria number was counted after 22~24 hr incubation from counting agar plate in which sperm dilute to $10{\sim}10^6$ in 0.9% saline solution and inoculate to agar. Acrosomal integrity was measured by Chlortetracycline (CTC) staining. CTC patterns were uniform fluorescence over the whole head (pattern F), characteristic of incapacitated acrosome-intact spermatozoa; fluorescence-free band in the post-acrosomal region (pattern B), characteristic of capacitated acrosome-intact spermatozoa; and almost no fluorescence over the whole head except for a thin band in the equatorial segment (pattern AR), characteristic of acrosome reacted spermatozoa. Total number of bacteria was significantly increased (p<0.0001) 3 days after preservation. Sperm motility, viability, and morphological abnormality on elapsed time after preservation were lower from 5 ($77.24{\pm}6.47$, p<0.001) and 7 days ($77.24{\pm}6.47$, p<0.001) after preservation compared to 1 ($15.71{\pm}7.18$) and 3 days($18.39{\pm}7.22$) after preservation, respectively. Sperm viability was significantly lower ($53.25{\pm}35.03$, p<0.0001) at 7 days after preservation. Morphological abnormality of sperm was lower (p<0.001) at 1 ($15.71{\pm}7.18$) and 3 ($18.39{\pm}7.22$) days compared to 5 ($21.84{\pm}7.91$) and 7 ($22.59{\pm}9.93$) days after preservation. Acrosomal integrity and capacitation rate (pattern F) were significantly lower (p<0.001) from 5 days after preservation. Based on the data we obtained from this study suggested that semen preserved more than 5 days without antibiotic would not recommend use for artificial insemination.

Research on Audiovisual Type Preservation Format Selection Criteria and Recommended Formats: Focusing on Audio Types (시청각 유형 보존포맷 선정기준 및 권고포맷 연구 - 오디오 유형을 중심으로 -)

  • Hanyeok Jeon;Dongmin Yang
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.35 no.1
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    • pp.273-300
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    • 2024
  • In the electronic records environment, along with discussions on ways to digitize analog records, it is important to prepare preservation strategies for each type of records produced and received electronically. In the same context, there is a need for discussion on applying a preservation format selection system with the goal of long-term preservation of data sets and audio-visual type electronic records other than document types. Audiovisual records must apply preservation strategies appropriate to the characteristics of each medium, such as images, audio, and video. This study establishes unique standards for selecting a preservation format for audio-visual electronic records through analysis of Significant Properties based on literature review, composed audio-type preservation format suitability evaluation items, and proposed a recommended format based on the results of applying them.

Do Physiognomically Designated Protected Areas Match Well with Ecological Data based upon Diversity Indices and Ordination? Implications for Urban Forest Conservation

  • Kee Dae Kim
    • Journal of Environmental Science International
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    • v.32 no.5
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    • pp.329-341
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    • 2023
  • We surveyed the vegetation of an ecological landscape preservation area (legally protected conservation areas or national parks) and the surrounding areas of Mt. Cheonggye, Republic of Korea, to explore the conservation implications for preservation areas and surrounding transition areas. We calculated diversity indices to identify the properties of the preservation and surrounding areas that are relevant to conservation efforts. We then compared the plant community composition between the areas using field and quadrat surveys in the preservation and surrounding areas. The cover of the dominant species in all tree and herb layers was markedly higher in the preservation area than in the peripheral zones. The species richness indices were significantly higher in the preservation area than in the peripheral zones. Ordination using detrended canonical correspondence analyses showed that the cover of the dominant tree species and rocks could explain the distribution of plant species in the Cartesian space of the ordination. Our results demonstrate that physiognomically designated protected areas match well with ecological data based on diversity indices and ordination analyses and that disturbances in the areas surrounding the ecological landscape of preservation areas can have considerable impacts on plant diversity indices. Hence, the preservation and management of surrounding areas are essential conservation elements for protecting the entire ecological landscape of preservation areas.

Preservation Environments of Cooperative Library Storage Facilities (공동보존서고의 보존환경에 관한 연구)

  • Cho, Yong-Wan;Rho, Jee-Hyun
    • Journal of Korean Library and Information Science Society
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    • v.44 no.4
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    • pp.367-397
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    • 2013
  • Recently, there are some discussions about cooperative library storage facilities as methods to relieve the pressure from shortage of library stacks in Korea. This study tried to inspect the preservation environments of 16 cooperative storage facilities in USA, Australia, France, Spain and Korea to help to prepare and establish cooperative storage facilities with ideal preservation conditions. To do this, some aspects of the preservation environments including basic conditions shelving system, HVAC(heating, ventilating and air conditioning) system, fire extinguishing system, security system and other preservation environments were compared and analyzed.

Management of the Preservation Media for Electronic Records (전자기록물을 위한 보존매체의 관리)

  • Song, Byoung-Ho
    • Journal of the Korean Society for Library and Information Science
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    • v.39 no.4
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    • pp.177-192
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    • 2005
  • Traditional paper records have two aspects such that the paper records are information, and are information media itself. The preservation media for the paper records seems to be another forms for original ones including microfilm and optical disks. On the other hand, new-coming electronic records lack the aspect of information media. To guarantee the existency of the original electronic records we need the preservation media for the electronic records, different from the media for the traditional paper records. The preservation media helps the Authenticity, integrity, and usability as well. This paper describes the need for the preservation media, identifies problems on the existing concept for them, derives the principles to resolve the problems, and suggests the management policy based on the principles.

A Study on the Digital Archiving Workflow and Preservation Techniques (디지털 아카이빙의 워크플로우와 보존처리 기술에 관한 연구)

  • Lee Soo-Sang
    • Journal of Korean Library and Information Science Society
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    • v.35 no.3
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    • pp.119-138
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    • 2004
  • The objective of digital preservation is to ensure that digital information survives in usable form for as long as it is wanted That is to ensure long-term access to digitally stored information. The preservation of digital objects involves a variety of challenges, including policy questions, institutional roles, legal and rights issues, metadata, and technical strategies. In this paper, I introduce the definition of digital object life-cycle of digital object and related archiving workflow which are basic concepts of digital archiving. Also I focus on the aspects of current techniques used in digital information preservation including technology preservation, emulation, refreshing, migration, encapsulation.

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