• 제목/요약/키워드: Preservation method

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충청남도 발굴유구 보존현황 및 보존방안 연구 (A Study on Present State of Preservation of Excavated Remains in Chungcheongnam-do and Preservation Methods)

  • 위광철;오승준;정제원
    • 보존과학회지
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    • 제33권6호
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    • pp.541-552
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    • 2017
  • 발굴된 모든 매장문화재는 보존조치의 필요성에 따라 문화재청장은 보존조치를 결정한다. 하지만 보존조치 결과에 따라 이전 복원된 유적의 관리에 대한 법적인 규정이 없는 상태로 인해 관리에 대하여 소홀한 실정이다. 이에 본 논문에서는 충청남도 지역을 대상으로 최근까지 보존조치되어 이전복원된 매장문화재의 보존현황 및 보존방안에 대하여 제시하였다. 충청남도에서 매장문화재 발굴 후 보존조치된 유적은 23개 유적 46개 유구가 확인되었다. 보존유형은 해체이전 27개 유구, 유구전사 14개 유구, 해체이전+유구전사 병행 2개 유구, 모형제작 3개 유구로 이루어졌다. 복원 설치된 유적의 지역별로는 부여군 9개 유적, 공주시 3개 유적으로 부여군과 공주시에 12개 유적 18개 유구가 집중적으로 분포되어 있고, 시대별로는 백제시대 유적이 16개 유적으로 약 70%를 차지하고 있었다. 보호유형은 노천형이 33개 유구로 약 72%를 차지하고 있는 것으로 확인되었다. 이전복원된 유적 관리 상태조사 결과 관리 주체가 명확한 박물관 등 공공기관은 관리를 위한 자체인력을 보유하고 있어 보존 관리가 잘 이루어지고 있었으나 관리 주체가 모호한 경우에는 관리 소홀로 인한 유구 손상이 심각한 것으로 나타났다. 이러한 문제점 해결을 위해 명확한 관리 주체의 명시, 주기적인 관리 및 점검, 이전 복원 방법에 대한 보존처리 방법 연구 등이 필요하다. 발굴된 매장문화재는 학술적 목적뿐만 아니라 홍보자료 및 전시자료로도 활용하고 있어 보다 철저한 관리를 통한 매장문화재의 활용이 필요하다고 판단된다.

Autographa californica 핵다면체 바이러스의 세포외 미봉입비리온의 안전한 보존 (A Stable Preservation of Extracellular Nonoccluded Virions from Autographa californica Nuclear Polyhedrosis Virus Infection)

  • 이형환;이근광;이건주
    • 한국미생물·생명공학회지
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    • 제18권6호
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    • pp.660-661
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    • 1990
  • Autographa californica nuclear polyhedrosis virus(AcNPV) L-1주의 extracellular nonoccluded virion (NOV)을 보관하는 방법을 연구하였다. AcNPV NOVs을 Spodoptera frugiperda cell line에 감염을 시킨 후에 배양액을 원심분리하여 AcNPV NOVs가 들어 있는 상등액을 취하여 $4^{\circ}C$에서 약 11년간 보관하였다. 보관되어 있는 AcNPV NOV을 Spodoptera frugiprda cell line에 재감염하여 관찰한 결과 NOVs의 감염과 증식이 정상적이었으며, NOV의 역가가 $8.9 \times 10^7$pfu/ml에서 $3.8 \times 10^5$pfu/ml로 떨어졌을 뿐이다. 또한 HindIII와 EcoRI 제한효소로 AcNPV genome DNA을 절단하여 패턴을 조사한 결과 DNA제한 효소 패턴은 변하지 않았다. 즉 AcNPV NOVs는 $4^{\circ}C$에서 보존하면 10년 이상 안정성이 있고, 취급이 용이하다는 것을 알 수 있었다.

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일측 폐이식 실험견에서 이식폐의 기능 평가연구 (An Assessment of Allograft Function in Canine Single Lung Transplantation)

  • 박창권
    • Journal of Chest Surgery
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    • 제28권12호
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    • pp.1096-1106
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    • 1995
  • We experienced 7 cases of left single lung transplantation in 14 mongrel dogs and analyzed graft lung function by hemodynamics, blood gas analysis, chest X-ray, biopsy and perfusion lung scan. We performed right pulmonary artery cuff[PA cuff for analysis of graft lung function in 3 cases. The donor lungs were flushed with modified Euro-Collins solution[n=3 or low potassium dextran glucose solution[n=4 and preserved for 4 to 5 hours[n=4 or 24 hours[n=3 at 10o C and implanted to the dogs with similar weight . Assessment of left graft lung was done by occluding the right pulmonary artery for 10 minutes using PA cuff. Assessment for graft lung function was done immediately after an operation and after 3 days, 7days and 3 weeks postoperatively. Four dogs survived for 3days, 7days[2 cases and 3 weeks respectively. Other three dogs expired within 3 hours of reperfusion. Immediate perfusion scans of left lung in four survived dogs after reperfusion were 42.1%, 36% , 11% and 5.9% respectively, and another dog with 4.8% perfusion to left lung was dead due to left atrial thrombi after 3 hours reperfusion. In one case among three acute rejections follow-up perfusion scan was done on 3rd and 11th postoperative day and the result decreased from 36% perfusion immediate postoperatively to 21% and 15% respectively. Three expired dogs postoperatively couldn`t tolerate occlusion of right pulmonary artery with above 40 mmHg of mean pulmonary artery pressure. On the other hand, three survival dogs postoperatively tolerated occlusion of right pulmonay artery with less than 30 mmHg of mean pulmonary artery pressure. and one dog couldn`t tolerate same procedure immediate postopertively but in 2 hours reperfusion later tolerated with 29 mmHg of mean pulmonary artery pressure.In conclusion we couldn`t compare the effect of two flushing solutions but low potassium dextran glucose solution showed relatively safe preservation effect in cases with preservation of more than 20 hours. Also canine left single lung transplantation model with PA cuff indicated useful method for the assessment of graft lung function with effect of lung preservation.

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승모판막 협착 질환에서 건삭보존 치환술에 대한 연구 (Mitral Valve Replacement with Chordal Preservation in Mitral Stenotic Disease)

  • 김태호;김공수;구자홍
    • Journal of Chest Surgery
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    • 제32권1호
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    • pp.10-15
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    • 1999
  • 배경: 건삭보존 승모판 치환술이 승모판 폐쇄부전증에 있어서는 좌심실 기능의 개선 및 술후 합병증을 줄일 수 있다고 알려져 있으나 승모판 협착증에 있어서는 좌심실 유출로의 폐쇄나 기계판엽의 운동장애 없이 적당한 크기의 기계판막을 삽입할 수 없다는 점등으로 아직 논란의 대상이 되고 있다. 대상 및 방법: 본원에서는 5명의 승모판 협착증환자와 7명의 승모판 폐쇄부전증 환자를 대상으로, 융합된 교련의 절개, 두꺼워진 판첨을 얇게 박리하고, 전판첨을 전판륜으로부터 2 mm 정도에 절개를 가하고 전판첨 중앙부를 절제하여 판엽을 두 개의 분절로 분리하여 교련부에 재 부착함으로써 건삭 및 판첨을 판륜에 위치이동 하면서 승모판막 치환술을 시행하여 건삭 및 판륜의 연속성을 유지하였다. 결과: 술후 기계판엽의 운동장애나 판막주위누출, 좌심실 유출로의 폐쇄 등 합병증 및 수술 사망환자는 없었다. 결론: 결론적으로 건삭보존 승모판 치환술이 승모판 협착증에 있어서도 안전하고 효과적인 수술방법이라 할 수 있다

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어류서식처 평가 및 목표종 도입을 통한 하천복원방향 - 부안댐 상류 거석천과 청림습지를 대상으로 - (Stream Restoration Guidelines by Evaluation of Fish Habitat and Introducing of the Keystone Species - Geo-seok Stream and Cheonglim Wetland in Upper Stream of Buan-Dam -)

  • 박선아;이명우
    • 한국환경복원기술학회지
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    • 제11권4호
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    • pp.24-36
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    • 2008
  • The essence of a natural stream restoration project is providing habitats for various creatures in terms of the stream's environmental function. This research suggests the problems of natural stream restoration project by studying streams in terms of habitats for creatures and reviewing the national and international cases on existing natural stream restoration project. As a solution for the above, this research suggests a preservation method for biotope of upper stream areas focusing on the fish biotope area considering the ecological characteristics and preservation value of the respective stream so that the stream can be preserved as habitats for creatures. The research was carried out on Cheonglimli Baekcheon region which is an upper stream area of Buan-Dam located in the public park in Byunsanbando, Buangoon, Jeonlabookdo. We suggest the preservation method based on the habitat environment and keystone species of fish. The habitat environment evaluation was carried out mainly being divided into three groups namely, habitats and surrounding environment, reservoir's features and fish way and the diversity of fish. By improving the problematic elements discovered through such evaluation, it tries to improve the biotope of the stream and continuously preserve the stream. In addition, in order to restore the stream as habitats for various creatures, this research suggests to select keystone species which are suitable for the stream environment and restore the habitats based on the keystone species rather than restoring habitats for all species. Fish not only plays the role of a consumer of Epilithic algae and water beetles but also plays an important ecological role as a food for birds or small mammalia. If such ecologically important biotope for fish is preserved, the ecological environment of the stream will be maintained more stably.

Preservation of Coagulation Efficiency of Moringa oleifera, a Natural Coagulant

  • Katayon, S.;Ng, S.C.;Johari, M.M.N.Megat;Ghani, L.A.Abdul
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제11권6호
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    • pp.489-495
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    • 2006
  • In recent years, there has been an interest to use Moringa oleifera as the natural coagulant due to cost, associated health and environmental concerns of synthetic organic polymers and inorganic chemicals. However, it is known that M. oleifera as the natural coagulant is highly biodegradable and has a very short shelf life. This research was carried out to investigate the effects of storage temperature, packaging methods, and freeze-drying on the preservation of M. oleifera seeds powders. Non freeze-dried M. oleifera was prepared into different packaging namely open container, closed container and vacuum packing, whilst, freeze-dried M. oleifera was stored in closed container and vacuum packing. Each of the packaging was stored at room temperature ($30\;to\;32^{\circ}C$) and refrigerator ($4^{\circ}C$). The turbidity removal efficiencies of stored M. oleifera were examined using jar test at monthly interval for 12 months. The results indicated that non freeze-dried M. oleifera kept in the refrigerator ($4^{\circ}C$) would preserve its coagulation efficiency. In addition, closed container and vacuum packing were found to be more appropriate for the preservation of non freeze-dried M. oleifera, compared to open container. Freeze-dried M. oleifera retained its high coagulation efficiency regardless the storage temperature and packaging method for up to 11 months. Besides, higher increment in zeta potential values for water coagulated with freeze-dried M. oleifera indicated the higher frequency of charge neutralization and better coagulation efficiency of freeze-dried M. oleifera, compared to non freeze-dried seeds. As a coagulant, M. oleifera did not affect the pH of the water after treatment.

중국경극(京劇)의 보전(保全)과 전승(傳承) - '중국경극음배상공정(中國京劇音配像工程)'에 대하여 (The studies on preservation and transmission of Jing-Ju - focused on the 'China Jing-Ju Yinpeixiang project')

  • 오경희
    • 비교문화연구
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    • 제25권
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    • pp.421-452
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    • 2011
  • This writing is about an effort to try to preserve and transmit the materials related to Jing-Ju(京劇) who is the Chinese traditional theatrical art. The plan to preserve the materials of performing arts is very important because its collection and preservation about them are much more particular than other genres. China got the great results in this field because of China Jing-Ju Yinpeixiang (中國京劇音配像) project. Jing-Ju Yinpeixiang project is a kind of project to restore and preserve the materials which reproduced Jing-Ju repertoire by inserting the performance method of Yinpeixiang (音配像: put together images to sounds) by later actors and actresses of Jing-Ju in recordings of 1950s to 1960s by remained famous ones. Also, this is the large scale culture project that more than 170 institutions and more than 3000 persons participated in during the total 21 years that it first started in 1985 and was completed in the end of 2006. Thanks to this project, China got to have the almost perfect materials which reproduced live performances to add the images of junior actors and actresses who accurately imitated performances of 115 famous actors and actresses including each type of role, schools and great actors from 1907 to the end of 20th century in their voices. This is the only method to restore the materials in this world and very creative. It's been 15 years since TV broadcasting first showed its fruition to Chinese people. The Chinese government gave the positive judgement on this project and prepared to hold the big event which celebrated itself this year. But very little is known of this process and there is no any study in Korea. This writing tried to clarify what Jing-Ju Yinpeixiang project is, what it has meant to Chinese people and what results it has gotten after it's been 15 years since the first show was on TV.

전자기록 장기보존 위험관리 사례 (A Case Study on the Risk Management for the Long-term Preservation Business Activities Related to Electronic Records)

  • 임진희
    • 기록학연구
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    • 제39호
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    • pp.3-43
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    • 2014
  • 이 논문에서는 2010년 국가기록원에서 DRAMBORA(Digital Repository Audit Method Based on Risk Assessment) 프레임워크를 응용하여 전자기록 장기보존 업무에 대해 점검했던 사례를 상세히 제시하고 있다. 국가기록원은 2010년 전자기록 장기보존 업무에 관련하여 총 44개의 위험요소를 도출하여 정의하였고, 업무담당자들의 평가를 거쳐 위험도 등급에 따라 분류하였으며, 고위험도를 지닌 2개의 위험요소에 대해서는 상세한 관리방안을 개발하였다. 이 논문에서는 NR04 '기록정보의 무결성 손실' 위험요소에 대한 사전관리방안, 사후관리방안, 비상대책조직, 재난선포 주체와 기준, 개인임무카드 개발 내역을 소개하고 있다. 이 논문 내용을 통해 기록관리자들은 위험관리 기법을 구체적으로 이해할 수 있을 것이며, 기록관리 기관들은 중요 업무관리 방법으로 위험관리 기법을 수용할지 점검하는데 참고할 수 있을 것이다.

배아 보존에 관한 합리적 제도 개선을 위한 연구 (A Study on the Rational Improvement of the Regulation and System about Embryo Preservation)

  • 백수진;문한나;박인경;차승현;박준석;이경훈;박춘선;조희수;김명희
    • 의료법학
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    • 제22권3호
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    • pp.57-95
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    • 2021
  • 생명윤리법에 따라 우리나라의 배아 보존기간은 최대 5년이다. 그러나 최근 관련 과학기술 발달 및 보존기간 제한이 배아생성권자 권리를 제한한다는 요구가 제기됨에 따라 본 연구는 국내·외 법률을 검토하고 쟁점을 도출하였다. 첫 번째 쟁점으로 보존된 배아는 임신목적 착상 시도를 그 목적으로 하며, 과학적 근거 등 충분한 정보에 근거한 숙고를 통해 배아생성권자의 자율성이 보호되도록 하는 것이 중요하다는 것이다. 두 번째는 배아 보존기관 관리 의무 관련 규정이 필요하다는 것이다. 세 번째는 배아의 특수한 지위를 고려하여, 배아 생성, 보존, 폐기 행위가 최소한의 범위에서 이루어지는 사회적 분위기를 형성하는 것이다. 이 쟁점을 바탕으로 합리적 제도 개선 방안 제안의 첫 번째는 구체적 보존기간을 법에 명시하는 것보다, 충분한 설명과 적절한 동의가 행사될 수 있는 환경조성 및 보존기간의 연장 사유 확대 우선 도입이다. 두 번째는 보존기관 관리의무 뿐 아니라 그 결과 나타날 수 있는 우려 등 현장 전반을 고려한 제도화가 필요하다는 것이며, 마지막은 연구목적 제공을 위한 이관, 타인의 임신목적 기증 등 배아의 향후 활용 방안을 고려한 관리 방식의 도입을 제안한다. 이 과정은 태어난 아이와 가족관계 등 전반적인 고찰 뿐 아니라, 충분한 사회적 논의와 합의를 통한 방식이 되어야 할 것이다.

저장 기간에 따른 스페셜티 등급 커피의 소비자 관능평가 (Consumer's Sensory Evaluation of Specialty Grade Coffee during Storage)

  • 권대중;이민준;박옥진
    • 한국식품영양학회지
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    • 제25권4호
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    • pp.1033-1038
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    • 2012
  • This study was done to provide basic information about the storage and distribution of specialty grade coffee of which the market will be expanded through customer sensory evaluation according to the storage period of specialty grade coffee by the SCAA classified method with consideration to defective beans and scent. The specialty grade coffee sample of this study was extracted on day 1, day 14, and day 28. The water content showed a significant reduction on the 28th storage day and there was no pH change according to the storage period. Color degree was brightened according to the preservation period before and after grinding but there was no significant difference. The panels for sensory evaluation considered of 24 university. The sensory evaluation consist of aroma, bitterness, and astringency, acidity, aftertaste, overall satisfaction, and items for strength estimation consist of aroma, acidity and bitterness. The sensory evaluation was expressed by applying a 5 point Likert scale. The results showed that as the storage period increased so too did the aroma and the strength of bitterness. Preference was the highest on day 1 in every items except aroma and astrigency. However on day 14 and day 28, most of the sensory evaluation became low and then became high, which was inconsistent. Male students were not consistent in their evaluation according to the preservation period, but female students rated aroma and bitterness the lowest on preservation period day 1 and they prefer after a storage period of 1 day in regards to the entire degree of satisfaction including sour test. astrigency and aftertaste. The analysis of visiting frequency for coffee shops used demonstrated that the longer the preservation period, the lower the preference but was not significant in the case of involving a little frequency group. The group that did not like Americano coffee evaluated that the longer the preservation period, the lower the preference based on Americano coffee preference analysis. There was no consistency between the storage period day 14 and day 28 in strength and preference, but preference for storage period day 1 was high in every group, which means that the quality of coffee is affected by the preservation period.