• The Transactions of The Korean Institute of Electrical Engineers
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• v.56 no.12
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• pp.2208-2213
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• 2007

In this study we have developed a hyperspectrum imaging system for highly sensitive and effective imaging analysis. An optical setup was designed using acoustic optical tunable filter (AOTF) for high sensitive hyperspectrum imaging. Light emitted by mercury lamp gets split in to diffracted and undiffracted beams while passing though AOTF. GFP transfected HEK-293 cell line was used as a model for in vitro imaging analysis. Cells were first, analyzed by fluorescence microscope followed by flow cytometric analysis. Flow cytometric analysis showed 66.31% transfection yield in GFP transfected HEK-293 cells. Various images of GFP transfected HEK-293 cell were grabbed by collecting the diffracted light using a CCD over a dynamic range of frequency of 129-171 MHz with an interval of 3 MHz. Subsequently, for in vivo image analysis of GFP transfected cells in mouse, a whole-body-imaging system was constructed. The blue light of 488 nm wavelength was obtained from a Xenon arc lamp using an appropriate filter and transmitted through an optical cable to a ring illuminator. To check the efficacy of the newly developed whole-body-imaging system, a comparative imaging analysis was performed on a normal mouse in presence and absence of Xenon arc irradiation. The developed hyperspectrum imaging analysis with AOTF showed the highest intensity of green fluorescent protein at 153 MHz of frequency and 494 nm of wavelength. However, the fluorescence intensity remained same as that of the background below 138 MHz (475 nm) and above 162 MHz (532 nm). The mouse images captured using the constructed whole-body-imaging system appeared monochromatic in absence of Xenon arc irradiation and blue when irradiated with Xenon arc lamp. Nevertheless, in either case mouse images appeared clearly.

• Journal of Embryo Transfer
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• v.27 no.3
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• pp.189-192
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• 2012

Although embryonic stem cells (ESCs) or ES-like cells are reported from many mammalian species other than the mouse, the culture system for murine ESCs may not be suitable to the other species. Previously many other research groups have modified either human or mouse ESC culture systems for bovine ESC culture. In this study, we compared three different culture mediums consisting of DMEM, ${\alpha}$-MEM or KnockOut$^{TM}$-DMEM (KO), which are modified from human or mouse ESC culture system, for the generation of bovine ESCs. In this study, some pre-requisite events which are important for establishment and long-term propagation of ESCs such as inner cell mass (ICM) attachment on feeder cells, primary colony formation and sustainability after passaging. Once the ICM clumps attached on feeder cells, this was designated as passage 0. In regards to the rate of ICM attachment, ${\alpha}$-MEM was superior to the other systems. For primary colony formation, there was no difference between DMEM and ${\alpha}$-MEM whereas KO showed lower formation rate than the other groups. For passaging, the colonies were split into 2~4 pieces and passed every 5~6 days. From passage 1 to passage 3, DMEM system seemed to be appropriate for maintaining putative bovine ESCs. On the other hand, ${\alpha}$-MEM tended to be more suitable after passage 6. Although ${\alpha}$-MEM support to maintain a ES-like cell progenies to passage 15, all three culture systems which are modified from human or mouse ESC culture media failed to retain the propagation and long-term culture of putative bovine ESCs. Our findings imply that more optimized alternative culture system is required for establishing bovine ESC lines.

• Journal of the Korean Society of Earth Science Education
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• v.5 no.1
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• pp.105-113
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• 2012

The purpose of this study was to examine the effects of storytelling skill on science learning motivation and space perception ability. For this study the 5 grade, 2 class was divided into a research group and a comparative group. The class was pre-tested in order to ensure the same standard. The research group had the science class with storytelling skill, and the comparative group had the class with teacher centered lectures for 10 classes in 10 weeks. The storytelling skill was focused on finding stories, constellation searching, story deciding, story hero deciding, story composition, storytelling completion. To prove the effects of this study, science learning motivation was split up according to attention power, relation, confidence, and sense of satisfaction. Also, space perception ability consisted of two-dimensional rotation, 3 dimension rotations, reflection, three-dimensional searching, number of block, and figure type in pattern. The results of this study are as follows. First, using storytelling skill was effective in science learning motivation. Second, using storytelling skill was effective in space perception ability. Also, after using storytelling skill was good reaction by students. As a result, the elementary science class with storytelling skill had the effects of developing science learning motivation and space perception ability. it means the science class with storytelling skill has potential possibilities and value to develop science learning motivation and space perception ability.

• Journal of the Korean Society of Earth Science Education
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• v.5 no.1
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• pp.51-59
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• 2012

The purpose of this study was to examine the effects of Future Problem Solving Program on creativity and scientific attitude. For this study the 4 grade, 2 class was divided into a research group and a comparative group. The class was pre-tested in order to ensure the same standard. The research group had the science class with FPSP, and the comparative group had the class with teacher centered lectures for 9 classes in 10 weeks. The FPSP was focused on finding problems, finding key problems, creating solutions, selecting the standard of judgement, making alternative solutions, and learning creative steps of solutions consisting of development of action planning. To prove the effects of this study, creativity was split up according to fluency, originality, abstractness, accuracy, and openness. Also, scientific attitude consisted of honesty, patience, curiosity, preparedness, autonomy, criticism, and openness. The results of this study are as follows. First, the science class with FPSP with finding problems, finding key problems, and creating solutions had the effect of developing the scientific creativity; fluency, originality, abstractness of the title, accuracy, and openness. Second, the FPSP had the effect of developing the scientific attitude. Students made ideas and solved the problems through divergent thinking and convergent thinking. During the class it had the effect of developing the scientific attitude; honesty, patience, curiosity, preparedness, autonomy, criticism, and openness. As a result, the elementary science class with FPSP had the effects of developing scientific creativity and scientific attitude. It means the science class with FPSP has potential possibilities and value to develop scientific creativity and scientific attitude.

• Imaging Science in Dentistry
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• v.32 no.4
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• pp.201-206
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• 2002

Purpose : To evaluate the radiographic change of operation sites after orthognathic surgery using the digital image processing and fractal analysis. Materials and Methods : A series of panoramic radiographs of thirty-five randomly selected patients who had undergone mandibular orthognathic surgery (bilateral sagittal split ramus osteotomy) without clinical complication for osseous healing, were taken. The panoramic radiographs of each selected patient were taken at pre-operation (stage 0), 1 or 2 days after operation (stage 1), 1 month after operation (stage 2), 6 months after operation (stage 3), and 12 months after operation (stage 4). The radiographs were digitized at 600 dpi, 8 bit, and 256 gray levels. The region of interest, centered on the bony gap area of the operation site, was selected and the fractal dimension was calculated by using the tile-counting method. The mean values and standard deviations of fractal dimension for each stage were calculated and the differences among stage 0, 1, 2, 3, and 4 were evaluated through repeated measures of the ANOVA and paired t-test. Results : The mean values and standard deviations of the fractal dimensions obtained from stage 0, 1, 2, 3, and 4 were 1.658±0.048, 1.580±0.050, 1.607±0.046, 1.624±0.049, and 1.641 ±0.061, respectively. The fractal dimensions from stage 1 to stage 4 were shown to have a tendency to increase (p < 0.05). Conclusion: The tendency of the fractal dimesion to increase relative to healing time may be a useful means of evaluating post-operative bony healing of the osteotomy site.

• Journal of Ginseng Research
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• v.30 no.4
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• pp.188-193
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• 2006

We induced the activation of melanocytes in the epidermis of C57BL/6 mice by ultraviolet B (UVB) irradiation and observed the effect of red ginseng (RG) on the formation, and decrease of UVB-induced epidermal mel-anocytes. C57BL/6 mice were irradiated by UVB $80mJ/cm^2$ (0.5 mW/sec) daily for 7 days, and RG was intraperitoneally or topically applied pre- or post-irradiation. For the estimation of change of epidermal melanocytes, light microscopic observation with dihydroxyphenylalanine (DOPA) stain was performed. Split epidermal sheets prepared from the ear of untreated mice exhibited 11-16 $melanocytes/mm^2$, and one week after UV irradiation, the applied areas show an increased number of strongly DOPA-positive melanocytes with stout dendrites. But intraperitoneal or topical treatment with RG before each irradiation interrupted UVB-induced pigmentation and resulted in a marked reduction in the number of epidermal melanocytes as compared to radiation control skin. The number and size of DOPA-positive epidermal mel-anocytes were also significantly decreased in intraperitoneally injected or topically applicated group after irradiation with RG at 3rd and 6th weeks after irradiation. The present study suggests the RG as inhibitor of UVB-induced pigmentation and depigmenting agent.

• Journal of the Korean Society of Earth Science Education
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• v.9 no.1
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• pp.97-105
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• 2016

The purpose of this study was to examine the effects of storytelling skill on science concept and science learning motivation. For this study the 5 grade, 2 class was divided into a research group and a comparative group. The class was pre-tested in order to ensure the same standard. The research group had the science class with storytelling skill, and the comparative group had the class of the teacher centered lectures on 11 classes in 8 weeks. The storytelling skill was focused on set the astronomical target wants to set up a story, through the small group discussion, present subject of the story, set the protagonist of the story for smooth configuration of the story, in order to smooth the flow of the story, make up a story around a hero, to make a clear story, decorated with pictures, shapes, graphs, etc, group story, complete with an astronomical(saints) in storytelling. To prove the effects of this study, science concept was split up according to knowledge, inquiry, attitude. Also, science learning motivation consisted of assignment is worth, learning beliefs about control, self efficacy. The results of this study are as follows. First, using storytelling skill was effective in science concept. Second, using storytelling skill was effective in science learning motivation. Also, after using storytelling skill was good reaction by students. As a result, the elementary science class with storytelling skill had the effects of developing science concept and science learning motivation. It means the science class with storytelling skill has potential possibilities and value to develop science concept and science learning motivation.

• Nuclear Engineering and Technology
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• v.41 no.9
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• pp.1143-1156
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• 2009

The SPACE code that is based on a multi-dimensional two-fluid, three-field model is under development for licensing purposes of pressurized water reactors in Korea. Among the participating research and industrial organizations, KAERI is in charge of developing the physical models and correlation packages for the constitutive equations. This paper introduces a developed wall-to-fluid heat transfer package for the SPACE code. The wall-to-fluid heat transfer package consists of twelve heat transfer subregions. For each sub-region, the models in the existing safety analysis codes and the leading models in literature have been peer reviewed in order to determine the best models which can easily be applicable to the SPACE code. Hence a wall-to-fluid heat transfer region selection map has been developed according to the non-condensable gas quality, void fraction, degree of subcooling, and wall temperature. Furthermore, a partitioning methodology which can take into account the split heat flux to the continuous liquid, entrained droplet, and vapor fields is proposed to comply fully with the three-field formulation of the SPACE code. The developed wall-to-fluid heat transfer package has been pre-tested by varying the independent parameters within the application range of the selected correlations. The smoothness between two adjacent heat transfer regimes has also been investigated. More detailed verification work on the developed wall-to-fluid heat transfer package will be carried out when the coupling of a hydraulic solver with the constitutive equations is brought to completion.

• Journal of the Korea Institute of Building Construction
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• v.19 no.4
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• pp.313-322
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• 2019

This study is to compare and analyze the physical and strength properties of lightweight concrete using domestic lightweight aggregate by replacement ratio of artificial lightweight fine and coarse aggregate after considering low cement mixture and pre-wetting time. The slump, unit weight, compressive strength and split tensile strength of lightweight concrete with domestic lightweight aggregate were measured. As test results, the slump of lightweight concrete by replacement ratio of lightweight fine aggregate increased as the replacement ratio of lightweight fine aggregate increased. The unit weight of lightweight concrete using 100% of lightweight fine aggregate was about 10.4% lower than that of the lightweight concrete with natural sand. In addition, the unit weight of lightweight concrete by replacement ratio of lightweight coarse aggregate increased with the increase of the ratio of LWG10(5~10mm). The compressive strength of lightweight concrete with lightweight fine and coarse aggregate increased as the replacement ratio of lightweight fine aggregate increased. The compressive strength of lightweight concrete with natural sand and LWG10 was 30 to 31MPa regardless of the replacement ratio of the lightweight coarse aggregate after 7 days.

• Advances in environmental research
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• v.9 no.4
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• pp.275-284
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• 2020

We provide the first study on the occurrence of microplastics in Dal lake, Kashmir, India. Microplastics act as catastrophe that trigger many environmental problems. The key origins of microplastics are larger plastics, which split into smaller plastics after UV light disintegration. There is relatively little work carried out on the existence of microplastics. The present work has been undertaken on the occurrence of microplastics at four pre-selected sites (surface water) in Dal lake, Kashmir. The samples were taken to the laboratory to dissolve organic matter by using H2O2 (6%). To speed up the organic digestion; the treated mixture was heated on a hot plate at 70℃. The mixture was then subjected to density separation. The supernatant obtained was observed under the microscope (10X) and measurements were taken. At site-I, the microplastics ranged from 2-3 mm, site-II 5-6 mm, site-III 3-4 mm, site-IV 4-5 mm in thickness, indicating the presence of microplastics in the lake. The presence of microplastics indicated that the lake has undergone an anthropogenic change over a period of time. Our research highlights the value of enhancing the quality of the drainage system and sewage disposal. This work can be helpful to recognize successful microplastic control management techniques and possible threats associated with the Dal lake. So far, no such data on the presence of microplastics in Kashmir lakes is available.