• KSBB Journal
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• 제15권1호
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• pp.55-59
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• 2000

생난황으로부터 시알산 함유 올리고당을 직접 생산하기 위한 biphasic system(water-immiscible hexane system)의 최적 조건을 조사하였다. Biphasic system I은 II보다 생산수율이 높았으며, 온도, 함수율 및 반응 시간의 최적조건은 각각 $30^{\circ}C$, 20%, 12시간이었다. Biphasic system I 에서, 난황을 가수분해하기 위한 3종류의 효소(protease A, corolase 7089, neutrase 0.5L)의 활성은 함수율과 온도에 강하게 영향을 받았으나, biphasic system II에서는 온도($30^{\circ}C$와 $50^{\circ}C$)의 영향을 거의 받지 않았다. 두 종류의 biphasic system에서 반응시간 12시간까지는 시알산 함유 올리고당의 생산량이 증가되었으나, 15시간 이후에는 거의 증가하지 않았다.

• 한국수소및신에너지학회논문집
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• 제23권6호
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• pp.619-627
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• 2012

This study was experimentally investigated on the effects of spring stiffness applied to linear compressor chambers. The springs prevented piston head from colliding with engine cover, stored the kinetic energy and regenerated the kinetic energy. The linear engine has two combustion chambers and four compressor chamber. The combustion chamber bore size was 30 mm, maximum stroke was 31 mm and effective stroke volume was 25.45 cc respectively. The spring stiffness was varied such as 0, 0.5, 1.00, 2.9 and 14.7 N/mm. The linear engine was fueled with premixed LPG (propane 99%) and air by pre-mixture device. As an experimental result, The stroke, piston velocity and the piston frequency were increased by high spring stiffness. Also, thermal efficiency was grown. because the increased stroke made the higher compression ratio. In conclusion, electric power and efficiency were improved.

• BMB Reports
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• 제38권5호
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• pp.517-525
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• 2005

Solid phase peptide synthesis method, which was introduced by Merrifield in 1963, has spawned the concept of combinatorial chemistry. In this review, we summarize the present technologies of solid phase peptide synthesis (SPPS) that are related to combinatorial chemistry. The conventional methods of peptide library synthesis on polymer support are parallel synthesis, split and mix synthesis and reagent mixture synthesis. Combining surface chemistry with the recent technology of microelectronic semiconductor fabrication system, the peptide microarray synthesis methods on a planar solid support are developed, which leads to spatially addressable peptide library. There are two kinds of peptide microarray synthesis methodologies: pre-synthesized peptide immobilization onto a glass or membrane substrate and in situ peptide synthesis by a photolithography or the SPOT method. This review also discusses the application of peptide libraries for high-throughput bioassays, for example, peptide ligand screening for antibody or cell signaling, enzyme substrate and inhibitor screening as well as other applications.

• Clinical and Experimental Reproductive Medicine
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• 제47권3호
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• pp.186-193
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• 2020

Objective: The effectiveness of Staphylococcus protein A (SPA) in improving the penetration ability of sperm and reducing antisperm antibody (ASA) titers in immunologically infertile males was evaluated. Methods: Seminal fluid samples were obtained from 15 infertile men, and ASA titers were assessed with the latex agglutination test. Identification of immunoglobulin (Ig) classes and characterization of the antigens involved in the immune response were performed using indirect immunofluorescence. Local ASAs typically present as a mixture of IgG and IgA classes. The capillary tube penetration method was used to assess the capability of spermatozoa to penetrate the cervical mucus (CM). Results: ASAs associated with the neck region of sperm showed a significantly lower migration distance in the CM of infertile females than ASAs associated with the head or tail segments. ASA-positive seminal fluid exhibited significant increases in the mean migration distance (2.6 ± 1.4 cm vs. 1.54 ± 1.1 cm, respectively; p< 0.001) and sperm concentration (174 ± 121.0 × 10³/mL vs. 101 ± 93.7 × 10³/mL, respectively; p= 0.033) after treatment with SPA compared to pre-treated samples. A significant reduction (p< 0.01) in the recorded ASA titer was detected. Conclusion: These results indicate that SPA can be used as a sorting regimen for insemination programs. However, further studies are warranted to assess its influence on pregnancy rate.

• 대한한의학회지
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• 제30권6호
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• pp.96-102
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• 2009

Objectives: Traditional Korean herbal formulae are composed of multiple medicinal plants. This situation of multiple-ingredient mixtures has been considered as a major obstacle to new drug development using herbal formulae in the world market, despite the effectiveness of such mixtures. This study reviewed Liv-52 as a representative model of successful drug development using a multiple-herb mixture. Methods: All articles for Liv-52 were collected from the PubMed database. The history, composition of Liv-52, its pharmaceutical efficacy and mechanisms, and data from clinical studies including its market size were analyzed. Results: Liv-52 is composed of seven herbal plants and it is the best known in Ayurvedic medicine for treating liver disorders. Since its 1955 introduction, forty four international papers have been published based on pre-clinical and clinical trials. The efficacy and mechanisms of Liv-52 were intensively studied. Currently, Liv-52 is one of the top-selling products, with over 10 million dollars sales annually, in the world market. Conclusions: These results indicate that Korean herbal formulae could be new global drugs if scientific evidence for efficacy and standardization are produced via literature researches.

• 한국분무공학회지
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• 제21권1호
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• pp.37-46
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• 2016

For the SI engines, at only full load, the pumping loss has a negligible effect, while at part load conditions, the pumping loss increases. To avoid the pumping loss, the spark-ignited engines are designed to inject gasoline directly into the combustion chamber. In the spark-ignited direct-injection engines, ignition probability is important for successful combustion and the flame propagation characteristics are also different from that of pre-mixed combustion. In this paper, a visualization experiment system is designed to study the ignition probability and combustion flame characteristics of spark-ignited direct-injection CNG fuel. The visualization system is composed of a combustion chamber, fuel supply system, air supply system, electronic control system and data acquisition system. It is found that ambient pressure, ambient temperature and ambient air flow velocity are important parameters which affect the ignition probability of CNG-air mixture and flame propagation characteristics and the injected CNG fuel can be ignited directly by a spark-plug under proper ambient conditions. For all cases of successful ignition, the flame propagation images were digitally recorded with an intensified CCD camera and the flame propagation characteristics were analyzed.

• 동력기계공학회지
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• 제20권2호
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• pp.32-42
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• 2016

Under part load condition of spark-ignition engine, pumping loss had great effect on engine efficiency. To reduce pumping loss, the study designed spark-ignited engines to make direct spray of gasoline to combustion chamber. In spark-ignited direct-injection engines, ignition probability is important for successful combustion and flame propagation characteristics are also different from pre-mixed combustion. This study designed a visualization testing device to study ignition probability of spark-ignited direct-injection LPG fuel and combustion flame characteristics. This visualization device consists of combustion chamber, fuel supply system, air supply system, electronic control system and data acquisition system. Ambient pressure, ambient temperature and ambient air flow velocity are important parameters on ignition probability of LPG-air mixture and flame propagation characteristics, and the study also found that sprayed LPG fuel can be directly ignited by spark-plug under proper ambient conditions. To all successful cases of ignition, the study recorded flame propagation image in digital method through ICCD camera and its flame propagation characteristics were analyzed.

• 한국분말재료학회지
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• 제7권1호
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• pp.35-41
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• 2000

Al2O3$.$SiC particle was prepared was prepared by the self-propagting high temperature sYthesis(SHS) process from a mixture of SiO2, Al and C powders, The fabricated Al2O3$.$SiC particle was applied to 2024Al/(Al2O3$.$SiC)pcomposite as a reinforcement. Aluminum matix composites were fabricares by the powder extrusion method using the synthesized Al2O3$.$SiC particle and commercial 2024Al powder. Theoptimum preparation conditions for Al2O3$.$SiC partticle by SHS process were described. The influence of the Al2O3$.$SiC voiume fraction on the mechanical was composite was also discussed. Despite adiabatic temperature was about 2367K, SHs reaction was completed not by itself, but by using pre-heating. Mean particle size of final particle synthesized was 0.73 ${\mu}$m and most of the particle was smaller than 2${\mu}$m. Elastic modulus and tensile strength of the composite increased with increase the volume fraction of reinforcement but, tensile strength depreciated at 30 vol% of reinforcement.

• 한국자동차공학회논문집
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• 제13권2호
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• pp.22-28
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• 2005

Exhaust gas emissions from internal combustion engines are one of the major sources of air pollution. And, it is extremely difficult to increase gasoline engine efficiency and to reduce $NO_X$ and PM(particulate matter) simultaneously in diesel combustion. This paper offers some basic concepts to overcome the above problems. To solve the problems, a recommended technique is CAI(controlled auto-ignition) combustion. In this paper, a flame trap was used to simulate internal EGR(exhaust gas recirculation) effect. An experimental study was carried out to find combustion characteristics using homogeneous premixed gas mixture in the constant volume combustion chamber(CVCC). Flame propagation photos and pressure signals were acquired to verify the flame trap effect. The flame trap creates high speed burned gas jet. It achieves higher flame propagation speed and more stable combustion due to the effect of geometry and burned gas jet.

• 한국자동차공학회논문집
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• 제19권5호
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• pp.29-34
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• 2011

This study investigates the potential of DME/Diesel dual fuel engine for reducing emissions with same power. Dual fuel engine controls the combustion using two different fuels, DME and diesel with different auto-ignition timings. In the previous work, the caracteristics of combustion and emissions under single cylinder engine and ignition is done by compression ignition. Pre-mixture is formed by injecting low-pressure DME into an intake manifold and high-pressure fuel (diesel or DME) is injected directly into the cylinder. Both direct diesel injection and port fuel injection reduced the significant amount of Smoke, CO and NOx in the homogeneous charge compression ignition engine due to present of oxygen in DME. In addition, when injecting DME directly in cylinder with port DME injection, there is no changes in emissions and energy consumption rate even operated by homogeneous charge compression ignition.