• BMB Reports
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• v.57 no.2
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• pp.79-85
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• 2024

Pre-harvest sprouting is a critical phenomenon involving germination of seeds in the mother plant before harvest under relative humid conditions and reduced dormancy. In this paper, we generated HDR mutant lines with one region SNP (C/T) and an insertion of 6 bp (GGT/GGTGGCGGC) in OsERF1 genes for pre-harvest sprouting (PHS) resistance using CRISPR/Cas9 and a geminiviral replicon system. The incidence of HDR was 2.6% in transformed calli. T₁ seeds were harvested from 12 HDR-induced calli and named ERF1-hdr line. Molecular stability, key agronomic properties, physiological properties, and biochemical properties of target genes in the ERF1-hdr line were investigated for three years. The ERF1-hdr line showed significantly enhanced seed dormancy and pre-harvest sprouting resistance. qRT-PCR analysis suggested that enhanced ABA signaling resulted in a stronger phenotype of PHS resistance. These results indicate that efficient HDR can be achieved through SNP/InDel replacement using a single and modular configuration applicable to different rice targets and other crops. This work demonstrates the potential to replace all genes with elite alleles within one generation and greatly expands our ability to improve agriculturally important traits.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.59 no.5
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• pp.847-853
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• 2010

This paper describes switching surge analysis for reclosing decision in 345kV combined transmission line with XLPE power cable. Reclosing operation should be decided based on the detailed technical analysis in combined transmission line because this line includes power cable section which is week on insulation. Insulation of power cable can be breakdown at the week point in case of reclosing moment. Therefore the detailed analysis has to be carried out by considering several conditions such as length ratio of power cable section, arrestor, inserting resistance, charging rate, grounding resistance, etc.. On the other hand sheath voltage on IJ(Insulated Joint) is analyzed to check dangerous condition on cable cover. Analysis is performed by EMTP/ATP. Analysis results show that reclosing can be operated as the single line-to-ground fault occurs on overhead line in 345kV combined transmission line, if the inserting resistance is considered before the operation of main circuit breaker.