• Journal of Life Science
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• v.17 no.2 s.82
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• pp.266-271
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• 2007

Bisphenol A (BPA) has been widely used as a monomer for production of epoxy resins and polycarbonate plastics. The annual production of BPA exceeds 640,000 metric tons in worldwide. BPA, a suspected phenolic endocrine disrupter, is moderately soluble and frequently detected in industrial wastewater. To date, HPLC and GC has been used for BPA analysis. However, HPLC and GC-analysis need high operation lost, experts, and an elaborate pre-treatment of samples, and is difficult to apply on-time and mass analysis. Therefore, simple, mass and rapid detection of BPA in environments is necessary. In the present study, spectrophotometric method of BPA quantification was developed. Based on blue-color product formation with BPA and ferric chloride/ferricyanide under the optimized conditions, the standard curve was acquired $({\lambda}_{750}=0.061\;BPA\;[{\mu}M]+0.07155,\;R^2=0.992)$. Using an established method, the BPA contents in the soil extract, and different water samples and living products, including disposable syringe, cup and plastic tube, were analyzed. The results suggested that the method is useful for BPA determination from different massive samples. Since the BPA metabolites, nontoxic 4-hydroxyacetophenone or 4-hydroxybenzaldehyde, did not form blue-color product, this method is also useful to screen a microorganism for BPA bioremediation.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.40 no.4
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• pp.666-677
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• 2015

The importance of application traffic analysis for efficient network management has been emphasized continuously. Snort is a popular traffic analysis system which detects traffic matched to pre-defined signatures and perform various actions based on the rules. However, it is very difficult to get highly accurate signatures to meet various analysis purpose because it is very tedious and time-consuming work to search the entire traffic data manually or semi-automatically. In this paper, we propose a novel method to generate signatures in a fully automatic manner in the form of sort rule from raw packet data captured from network link or end-host. We use a sequence pattern algorithm to generate common substring satisfying the minimum support from traffic flow data. Also, we extract the location and header information of the signature which are the components of snort content rule. When we analyzed the proposed method to several application traffic data, the generated rule could detect more than 97 percentage of the traffic data.

• The Journal of the Acoustical Society of Korea
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• v.22 no.5
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• pp.375-382
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• 2003

Lip-reading technique based on bimodal is to enhance speech recognition rate in noisy environment. It is most important to detect the correct lip-image. But it is hard to estimate stable performance in dynamic environment, because of many factors to deteriorate Lip-reading's performance. There are illumination change, speaker's pronunciation habit, versatility of lips shape and rotation or size change of lips etc. In this paper, we propose the IIR filtering in time-domain for the stable performance. It is very proper to remove the noise of speech, to enhance performance of recognition by digital filtering in time domain. While the lip-reading technique in whole lip image makes data massive, the Principal Component Analysis of pre-process allows to reduce the data quantify by detection of feature without loss of image information. For the observation performance of speech recognition using only image information, we made an experiment on recognition after choosing 22 words in available car service. We used Hidden Markov Model by speech recognition algorithm to compare this words' recognition performance. As a result, while the recognition rate of lip-reading using PCA is 64%, Time-domain filter applied to lip-reading enhances recognition rate of 72.4%.

• YAKHAK HOEJI
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• v.51 no.2
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• pp.96-102
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• 2007

Measurement of globotriaosylceramide (Gb3, ceramide trihexoside) in urine has clinical importance for monitoring after enzyme replacement therapy in Fabry disease patients. The disease is an X-linked lipid storage disorder that results from a deficiency of the enzyme ${\alpha}$-galactosidase A (${\alpha}$-Gal A). The lack of ${\alpha}$-Gal A causes an intracellular accumulation of glycosphingolipids, mainly Gb3. A simple, rapid, and highly sensitive analytical method for Gb3 in urine was developed without labor-extensive pre-treatment by electrospray ionization MS/MS (ESI-MS/MS). Only simple 5-fold dilution of urine is necessary for the extraction and isolation of Gb3 in urine. Gb3 in diluted urine was dissolved in dioxane containing C17:0 Gb3 as an internal standard. After centrifugation it was directly injected and analyzed through guard column by in combination with multiple reaction monitoring mode of ESI-MS/MS. Eight isoforms of Gb3 were completely resolved from urine matrix. C24:0 Gb3 occupied 50% of total Gb3 as a major component in urine. Linear relationship for Gb3 isoforms was found in the range of 0.005${\sim}$5.0 ${\mu}$g/ml. The limit of detection (S/N=5) was 0.005 ${\mu}$g/ml and limit of quantification was 0.05 ${\mu}$g/ml for C24:0 Gb3 with acceptable precision and accuracy. Correlation coefficient of calibration curves for 8 Gb3 isoforms ranged from 0.9598 to 0.9975. This method could be useful for rapid and sensitive 1st line Fabry disease screening, monitoring and/or diagnostic tool for Fabry disease.

• Journal of Biomedical Engineering Research
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• v.18 no.3
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• pp.301-306
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• 1997

Surgical removal of hepatic cancerous tissues have been widely performed due to its early detection. However, a patient can not survive if excessive hepatic tissues were removed. Therefore, quantitative evaluation of remaining hepatic function after surgery is a really important factor for surgeon. the currently used ICG Rmax and Lidocaine clearance tests have disadvantages such as tedium, complexity, and inability to estimate remaining hepatic function after surgery. While HEF has advantages such as simplicity, quickness, nonivasiveness, and quantification, its reliability has been doubtful. Thus, the program for calculation of HEF has been developed from serial gamma camera image data. And we compared the reliability of HEF with ICG Rmax and Lidocaine clearance test using 6normal and 18 abnormal rabbits with damaged livers. The correlation coefficient of HEF to ICG Rmax and MEGX was 0.91, 0.94, respectively. I was also found that the HEFs of normal and abnormal hepatic tissues was higher than 100% and lower than 80%, respectively. Thus we confirmed that HEF can be a good indicator distinguishing between abnormal tissues and normal ones. Finally, we could conclude that patients would survive if both the pre-and the post-operative HEF were greater than 60%.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.42 no.3
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• pp.649-660
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• 2017

In electronic warfare, the pulse amplitude, one of information of a pulse signal emitted by an enemy, is used for estimating distance from the source and for deinterleaving mixed source signals. An estimate of pulse amplitude is conventionally determined as the maximum magnitude of a Fourier transformed signal within its pulse width which is estimated pre-step in an electronic warfare receiver. However, when frequency modulated signals are received, it is difficult to estimate their pulse amplitudes with this conventional method because the energy of signals is dispersed in frequency domain. In order to overcome this limitation, this paper proposes an enhanced pulse amplitude estimation method which calculates the average power of the received pulse signal in time domain and removes the noise power of the receiver. Simulation results show that even in case the frequency modulated signal is received, the proposed method has the same performance as estimating the pulse amplitude when unmodulated signal is received. In addition, the proposed method is shown to be more robust to an estimation error of pulse width, which affects the estimation performance of pulse amplitude, than the conventional method.

• The Journal of Engineering Geology
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• v.16 no.2 s.48
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• pp.145-152
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• 2006

Industry development caused numerical and quantitative increase of noxious chemical substances that contain risk assessment in water resources. For use of efficient water resources a pre-treatment of contaminant source which is flowed in water resources is recognized in essential process. Therefore, the discharged water quality from discharged company began to control contaminant by total amount of pollutant in domestic. However, to estimate closely chemical substances it is not proved up to now, monitoring is very important. This study achieved a monitoring of micro noxious chemical substance by fiber and chemistry industrial wastewater inflow to examine risk assessment of the water system of Nakdong river. Chloroform was measured highest among volatile organic compounds (VOCs) that the results of water quality of influent and effluent are detected from 7 companies of study area. The other side, because measured value of detected chloroform is indefinite detection level in the same company, it is difficulty in management of water quality. However it may not be much effects of the water system of Nakdong river because these company's effluent is high treatment efficiency of chloroform (more than 88%) in sewage treatment plant. On the other hand, in the investigated results for the European Union specified priority substance that is detected to relationship influent and effluent from fiber/chemistry associated industries, these substances were not detected and domestic data was hardly referred. Therefore, data construction of continuous monitoring about this water quality may have to be achieved certainly to utilize as country policy.

• Journal of the Korean Society for Marine Environment & Energy
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• v.8 no.4
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• pp.179-185
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• 2005

Polycyclic aromatic hydrocarbons (PAHs) are a kind of toxic environmental pollutants and has been accumulated usually in marine sediments. Due to their potential hazardous to human, removal of PAHs from environments has been great concern. In the present study, the effect of microbial inoculation and the supplementation of mixed form cyclodextrin (M-CD) was assessed in the pre-sterilized or nonsterilized microcosms for optimizing operational conditions for ex situ bioremediation of sediments contaminated by PAHs. Activity of electron transport system (ETSA) was increased by the addition of M-CD regardless of inoculation of microorganisms in microcosms without sterilization. The degradation rate of PAHs in sterilized microcosms was app. 9-20% by the inoculation of single strain and 24-37% by the inoculation of microbial consortium supplemented with 1% M-CD, respectively. The degradation was not observed in microcosms without sterilization under the same conditions. The proportion of inoculated microorganisms also decreased in nonsterilized microcosms. Signals of inoculated bacteria were decreased to detection limit after 2 days in the microcosms without M-CD. In conclusion, microbial inoculation with appropriate carbon sources and removal of natural flora and grazers are required for the efficient ex situ bioremediation of sediments contaminated by PAHs in bioslurry reactor.

• Journal of Animal Science and Technology
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• v.49 no.4
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• pp.451-458
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• 2007

This study was to investigate the effectiveness of pre-weaning injection of gonadotropin and scheduled fixed-time insemination on sow fertility. Sows were randomly assigned to receive gonadotropin 2 days before weaning, 1 day after weaning or none as control. In gonadotropin treated groups, half of sows were inseminated twice at 24 and 36 hours after onset estrus and half of sows were inseminated twice at scheduled fixed-time. Weaning to onset of estrus was the shortest in lactating sows injected with gonadotropin prior to weaning. Liter size was significantly higher in AI groups after detection of estrus, compared to fixed-time AI group. Results of these experiments indicated that injection of gonadotropin in lactating sows could initiate the final follicular development, and has potential to enhance the reintegration of estrus. Further researches are needed to define the relationship between reduced interval of wean to onset estrus and enhanced fertility in lactating sows.

• Journal of Food Hygiene and Safety
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• v.29 no.4
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• pp.285-291
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• 2014

Saflufenacil is a low-volatile and uracil-based herbicide. This herbicide is used for pre-and post-emergence control of major broadleaf weeds. The objective of present study was to develop and validate an analytical method for saflufenacil determination in agricultural products for ensuring the food safety. The saflufenacil residues in samples were extracted with acetone, dichloromethane, and then purified with silica and graphitized carbon cartridge. The purified samples were analyzed by HPLC-UVD and confirmed with LC-MS. The linear range of saflufenacil was $0.1{\sim}5.0{\mu}gmL^{-1}$ with the correlation coefficient (r) = 0.999. Average recoveries of saflufenacil ranged from 80.5% to 110.2% at the spiked level of $0.02{\sim}0.5mgkg^{-1}$, while the relative standard deviation was 0.3~7.3%. In addition, the limit of detection and limit of quantification were 0.005 and $0.02mgL^{-1}$, respectively. Furthermore, an interlaboratory study among three labs was conducted to validate the method, and the results were satisfactory.