• 한국동물생명공학회지
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• 제35권4호
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• pp.307-314
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• 2020

In the present study, we examined the effect of straw size on spermatozoa motility, viability, acrosome integrity, mitochondrial membrane potential, and plasma membrane integrity after freezing-thawing. Hanwoo semen was collected from three bulls and diluted with an animal protein-free extender, divided into two groups, namely, 10 million spermatozoa in 0.25 mL and 20 million spermatozoa in 0.5 mL straw, and cryopreserved. In Experiment 1, the motility and motility parameters of the frozen-thawed spermatozoa were evaluated. After freezing-thawing, the spermatozoa motility parameters fast progressive, straight line velocity, and average path velocity were compared between the 0.25 mL straw and 0.5 mL straw groups. They were 35.2 ± 1.0 and 32.3 ± 0.7%, 34.6 ± 0.7 and 31.8 ± 0.5 μm/s, 51.4 ± 1.3 and 47.1 ± 1.1 μm/s, 0.25 mL straw and 0.5 mL straw groups, respectively. In Experiment 2, the viability, acrosome membrane integrity, and mitochondrial membrane potential of the frozen-thawed spermatozoa were assessed. After freezing-thawing, the percentages of spermatozoa with live, intact acrosomes and high mitochondrial membrane potential were compared between the in 0.25 mL straw and 0.5 mL straw groups. They were 48.0 ± 2.6% and 35.6 ± 2.8% between the 0.25 mL straw and 0.5 mL straw groups. In Experiment 3, the plasma membrane integrity of frozen-thawed spermatozoa was compared. After freezing-thawing, the plasma membrane integrity was higher for the in 0.25 mL straw group than the 0.5 mL straw group. They were 62.0 ± 2.2 and 54.1 ± 1.3% between the 0.25 mL straw and 0.5 mL straw groups. In conclusion, our results suggest that freezing semen in 0.25 mL straw improves the relative motility, viability, and acrosomal, mitochondrial membrane potential, and plasma membrane integrity of Hanwoo bull spermatozoa.

• 방사성폐기물학회지
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• 제21권3호
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• pp.411-417
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• 2023

During the operation of a nuclear power plant (NPP), the generation of radioactive waste, including dry active waste (DAW), concentrates, spent resin, and filters, mandates the implementation of appropriate disposal methods to adhere to Korea's waste acceptance criteria (WAC). In this context, this study investigates the potential use of polymer concrete (PC) as a high-integrity container (HIC) material for solidifying and packaging these waste materials. PC is a versatile composite material comprising binding polymers, aggregates, and additives, known for its exceptional strength and chemical stability. A comprehensive analysis of PC's long-term integrity was conducted in this study. First, its compressive strength, which is crucial for ensuring the structural stability of HICs over extended periods, was evaluated. Subsequently, the resilience of PC was tested under various stress conditions, including biological, radiological, thermal, and chemical stressors. The findings of this study indicate that PC exhibits remarkable long-term properties, demonstrating exceptional stability even when subjected to diverse stressors. The results therefore underscore the potential viability of PC as a reliable material for constructing high-integrity containers, thus contributing to the safe and sustainable management of radioactive waste in NPPs.

• Asian Pacific Journal of Cancer Prevention
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• 제17권3호
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• pp.939-944
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• 2016

Background: Cell-free DNA circulating in blood is a candidate biomarker for malignant tumors. Unlike uniformly truncated DNA released from apoptotic non diseased cells, DNA released from necrotic cancer cells varies in size. Objectives: To measure the DNA integrity index in serum and the absolute DNA concentration to assess their clinical utility as potential serum biomarkers for colorectal carcinoma (CRC) compared to CEA and CA19-9. Materials and Methods: Fifty patients with CRC, 10 with benign colonic polyps and 20 healthy sex and age matched volunteers, were investigated by real time PCR of ALU repeats (ALU q-PCR) using two sets of primers (115 and 247 bp) amplifying different lengths of DNA fragments. The DNA integrity index was calculated as the ratio of q-PCR results of ALU 247/ALU 115bp. Results: Serum DNA integrity was statistically significantly higher in CRC patients compared to the benign and control groups (p<0.001). ROC curves for differentiating CRC patients from normal controls and benign groups had areas under curves of 0.90 and 0.85 respectively. Conclusions: The DNA integrity index is superior to the absolute DNA concentration as a potential serum biomarker for screening and diagnosis of CRC. It may also serve as an indicator for monitoring the progression of CRC patients. Combining CEA and CA19-9 with either of the genetic markers studied is better than either of them alone.

• Journal of Positioning, Navigation, and Timing
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• 제9권3호
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• pp.285-292
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• 2020

This paper introduces a new computational efficient Dilution of Precision (DOP)-based landmark exclusion method while ensuring the safety of the LiDAR-based navigation system that uses an innovation-based Nearest-Neighbor (NN) Data Association (DA) process. The NN DA process finds a correct landmark association hypothesis among all potential landmark permutations using Kalman filter innovation vectors. This makes the computational load increases exponentially as the number of landmarks increases. In this paper, we thus exclude landmarks by introducing DOP that quantifies the geometric distribution of landmarks as a way to minimize the loss of integrity performance that can occur by reducing landmarks. The number of landmarks to be excluded is set as the maximum number that can satisfy the integrity risk requirement. For the verification of the method, we developed a simulator that can analyze integrity risk according to the landmark number and its geometric distribution. Based on the simulation, we analyzed the relationship between DOP and integrity risk of the DA process by excluding each landmark. The results showed a tendency to minimize the loss of integrity performance when excluding landmarks with poor DOP. The developed method opens the possibility of assuring the safety risk of the Lidar-based navigation system in real-time applications by reducing a substantial amount of computational load.

• 한국콘텐츠학회논문지
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• 제9권7호
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• pp.268-275
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• 2009

우리나라는 범국가적 차원에서 2002년 국가청렴위원회(과거 부패방지위원회)를 출범하여 부패방지를 위한 과학적이고 체계적인 접근 방법과 법률적 제도적 장치를 마련하고 부패의 사전 예방을 위한 홍보 및 교육프로그램을 운용하고 있으며, 공공기관별 부패 취약분야 및 유방요인을 파악하여 공공기관의 자율적인 반부패 대책수립과 개선노력을 적극 유도 하고 있다. 이에 따라 경찰청에서는 다양한 강도 높은 자체 사정활동과 부단한 윤리교육의 실시, 경찰공무원 행동강령 제정 등 다양한 부패방지 시책을 발굴하고 시행하여 왔음에도 불구하고 경찰에 대한 일반 국민은 아직도 경찰의 청렴도에 대하여 부정적인 평가를 하고 있는 실정이다. 경찰이 일반 국민들에게 청렴도에 있어서 부정적인 평가를 받고 있는 이유는 금품 향응 수수와 직결되는 부패인식과 부패경험이라는 체감청렴도보다 기준절차의 현실성, 업무태도, 업무처리의 공정성 등과 같은 잠재청렴도와 밀접한 관계가 있다. 실제 국가청렴위원회에서 조사한 대국민인식도 조사에서 경찰청이 낮은 지수를 받은 것도 체감청렴도에 비해 잠재청렴도의 중요성에 대한 인식이 부족하여 종합적 첨렴도지수를 향상시키지 못한 가장 큰 이유이다. 본 연구에서는 잠재청렴도를 향상시키기 위하여 경찰공무원 행동강령 준수의식의 정착, 민원 모니터링 확대 실시, 공정한 업무처리에 대한 신뢰 확보, 업무행태의 자가진단 시스템 도입, 온라인 고객평가 시스템 구축 등에 관하여 고찰함으로써 잠재청렴도 향상방안을 알아보고자 하였다.

• Animal Bioscience
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• 제35권2호
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• pp.166-176
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• 2022

Objective: Sperm is particularly susceptible to reactive oxygen species (ROS) stress. Glutathione (GSH) is an endogenous antioxidant that regulates sperm redox homeostasis. However, it is not clear whether boar sperm could utilize cysteine for synthesis GSH to protect sperm quality from ROS damage. Therefore, the present study was undertaken to elucidate the mechanism of how cysteine is involved in protecting boar sperm quality during liquid storage. Methods: Sperm motility, membrane integrity, lipid peroxidation, 4-hydroxyIlonenal (4-HNE) modifications, mitochondrial membrane potential, as well as the levels of ROS, GSH, and, ATP were evaluated. Moreover, the enzymes (GCLC: glutamate cysteine ligase; GSS: glutathione synthetase) that are involved in glutathione synthesis from cysteine precursor were detected by western blotting. Results: Compared to the control, addition of 1.25 mM cysteine to the liquid storage significantly increased boar sperm progressive motility, straight-line velocity, curvilinear velocity, beat-cross frequency, membrane integrity, mitochondrial membrane potential, ATP level, acrosome integrity, activities of superoxide dismutase and catalase, and GSH level, while reducing the ROS level, lipid peroxidation and 4-HNE modifications. It was also observed that the GCLC and GSS were expressed in boar sperm. Interestingly, when we used menadione to induce sperm with ROS stress, the menadione associated damages were observed to be reduced by the cysteine supplementation. Moreover, compared to the cysteine treatment, the γ-glutamylcysteine synthetase (γ-GCS) activity, GSH level, mitochondrial membrane potential, ATP level, membrane integrity and progressive motility in boar sperm were decreased by supplementing with an inhibitor of GSH synthesis, buthionine sulfoximine. Conclusion: These data suggest that boar sperm could biosynthesize the GSH from cysteine in vitro. Therefore, during storage, addition of cysteine improves boar sperm quality via enhancing the GSH synthesis to resist ROS stress.

• Structural Engineering and Mechanics
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• 제78권4호
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• pp.403-411
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• 2021

Ties are mandated by many design guidelines and codes to prevent the progressive collapse of buildings initiated by local failures. This study develops a model to estimate catenary/cable action capacity and the required ties in continuous reinforced concrete beams to bridge above the potential failed interior columns. The developed model is derived based on virtual work method and verified using test results presented in the literature. Also, parametric investigations are conducted to estimate the required ties in continuous reinforced concrete beams supporting one-way slab systems. A comparison is conducted between the estimated tie reinforcement using the developed model and that provided by satisfying the integrity provisions of the ACI 318-14 (2014) code. It is shown that the required tie reinforcements to prevent progressive collapse using the developed model are obviously larger than that provided by the integrity requirements of the ACI 318-14 (2014) code. It has been demonstrated that the increases in the demanded tie reinforcements over that provided by satisfying ACI 318-14 (2014) integrity provisions are varied between 1.01 and 1.46.

• 정보보호학회논문지
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• 제18권5호
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• pp.181-188
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• 2008

E-Science Grid is designed to cope with computation-intensive tasks and to manage a huge volume of science data efficiently. However, certain tasks may involve more than one grid can offer in computation capability or incur a long wait time on other tasks. Resource sharing among Grids can solve this problem with proper processing-integrity check via audit. Due to their computing-intensive nature, the processing time of e-Science tasks tends to be long. This potential long wait before an audit failure encourages earlier audit mechanism during execution in order both to prevent resource waste and to detect any problem fast. In this paper, we propose a Light-weight, Adaptive and Reliable Audit, LARA, of processing Integrity for e-Science applications. With the LARA scheme. researchers can verify their processing earlier and fast.

• The Korean Journal of Physiology and Pharmacology
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• 제28권5호
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• pp.393-401
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• 2024

Large language models (LLMs) are rapidly transforming medical writing and publishing. This review article focuses on experimental evidence to provide a comprehensive overview of the current applications, challenges, and future implications of LLMs in various stages of academic research and publishing process. Global surveys reveal a high prevalence of LLM usage in scientific writing, with both potential benefits and challenges associated with its adoption. LLMs have been successfully applied in literature search, research design, writing assistance, quality assessment, citation generation, and data analysis. LLMs have also been used in peer review and publication processes, including manuscript screening, generating review comments, and identifying potential biases. To ensure the integrity and quality of scholarly work in the era of LLM-assisted research, responsible artificial intelligence (AI) use is crucial. Researchers should prioritize verifying the accuracy and reliability of AI-generated content, maintain transparency in the use of LLMs, and develop collaborative human-AI workflows. Reviewers should focus on higher-order reviewing skills and be aware of the potential use of LLMs in manuscripts. Editorial offices should develop clear policies and guidelines on AI use and foster open dialogue within the academic community. Future directions include addressing the limitations and biases of current LLMs, exploring innovative applications, and continuously updating policies and practices in response to technological advancements. Collaborative efforts among stakeholders are necessary to harness the transformative potential of LLMs while maintaining the integrity of medical writing and publishing.

• 한국전자파학회지:전자파기술
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• 제24권2호
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• pp.51-57
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• 2013

The current expanding mobile markets incessantly demands small form factor, low power consumption and high aggregate throughput for silicon-level integration such as memory to logic system. One of emerging solution for meeting this high market demand is 3D through silicon stacking (TSS) technology. Main challenges to bring 3D TSS technology to the volume production level are establishing a cost effective supply chain and building a reliable manufacturing processes. In addition, this technology inherently help increase number of IOs and shorten interconnect length. With those benefits, however, potential signal and power integrity risks are also elevated; increase in PDN inductance, channel loss on substrate, crosstalk and parasitic capacitance. This paper will report recent progress of wide IO memory to high count TSV logic device assembly development work. 28 nm node TSV test vehicles were fabricated by the foundry and assembled. Successful integration of memory wide IO chip with less than a millimeter package thickness form factor was achieved. For this successful integration, we discussed potential signal and power integrity challenges. This report demonstrated functional wide IO memory to 28 nm logic device assembly using 3D package architecture with such a thin form factor.