• 한국버섯학회지
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• 제10권3호
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• pp.109-114
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• 2012

느타리 버섯류의 새로운 품종을 개발하기 위하여 고품질의 짙은 회색느타리 신품종을 육성하였다. 2003년부터 2004년까지 느타리 유전자원의 특성검정을 하였다. 2008년에 육종모본수한과 '농기201호'의 단핵체간 교잡하여 04-154 교잡주를 육성하였다. 이 교잡주 04-154와 품종 '청풍'의 단핵체와 다시 교잡하여 우수한 Po2008-275를 선발하여 특성검정, 확대재배를 실시하여 농작물 직무육성 신품종 선정심의회에서 '구슬'로 명명되었다. 주요특성으로 균사 생장 적온이 $25{\sim}30^{\circ}C$이며 버섯 원기형성 및 발생 온도는 $10{\sim}16^{\circ}C$이였다. 자실체의 갓 색깔은 짙은 회색으로 자연 상태에서 봄, 가을에 재배가 알맞은 특성을 가지고 있다. 균사체 배양기간은 25~30일이며 균 긁기 후 초발이소요일수는 3~5일로 온도가 높을수록 단축된다. 자실체 형태는 얕은 깔때기형이다. 대굵기는 $16.8{\pm}1.6mm$, 대길이는 $51.0{\pm}4.4mm$로 다른 느타리 종에 비해 자실체 대가 굵고 다소 짧은 편이다. 자실체 수량은 상자 당($43{\times}43{\times}11cm$) 1,545g으로 04-154 계통친이 100일 때 '구슬' 137, '청풍'이 146이었다. 가변특성으로는 감자배지와 버섯완전배지에서 균사를 배양한 결과 버섯완전배지에서 생장이 양호하였다. 또한 3종류의 primer를 이용하여 새로운 품종 '구슬'과 모균주에 대한 DNA profile을 분석한 결과 primer URP 1, primer URP 2, primer URP 5에서 양친주의 주요 밴드를 가지며 대조구인 '춘추2호'와는 뚜렷하게 구분되었다. 신품종 느타리 '구슬'은 소비자들이 가장 선호하는 짙은 회색의 갓을 나타내어 고품질을 요구하는 소비자를 만족시키는데 기여 할 것으로 기대된다.

• 식물조직배양학회지
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• 제27권5호
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• pp.415-421
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• 2000

The disaccharide trehalose ($\alpha$-D-glucopyranosyl-$\alpha$-D-glucopyranoside) is found in variety of organ-isms that are able to withstand almost complete desiccation. In order to identify the function of trehalose in plants, we isolated Arabidopsis trehalase (AtTRE) gene that encodes the enzyme able to hydrolyze trehalose to glucose, and trehalose-6-phosphate synthase isolog, TPS3 gene by RT-PCR. The AtTRE had the substrate specificity to hydrolyze only trehalose, and a broad pH range of enzyme activity. The AtTRE promoter/GUS reporter gene was expressed in cotyledons, mature leaf tissues including guard cells, and developing siliques. The GUS expression driven by AtTPS3 promoter was significant in root tissues, and the level of GUS activity was much higher than that of the pBll 21 control seedlings. The knockout of AtTPS3 gene in Arabidopsis resulted in the retarded root development, whereas the overexpression of AtTPS3 increased the root elongation in the presence of sucrose in MS medium. Possible functions of AtTRE and AtTPS3 in plant will be discussed. In addition, ectopic expression of yeast TPS1 driven by the inducible promoters in tobacco and potato conferred the plants on the drought and freezing tolerances.

• 동아시아식생활학회지
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• 제19권5호
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• pp.761-768
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• 2009

This study evaluated on the recognition and satisfaction for functional bakery products by consumers living in Seoul, Overall, 82.0% answered of the respondents reported that they had 'yes' which was 4.5 times as much as those who answered 'no' tried functional bakery products. Purchasing mostly depended on 'myself' (69.0%), and other methods of acquiring information as well as 'bakery' (10.8%), 'neighbour' (8.5%), 'media' (7.9%), and the 'internet' (3.7%). The preferred functional ingredients were 'sweet potato' with (22.9%), and 'rye and oat' (13.8%), 'green tea' (13.5%), 'pumpkin' (11.4%), and 'chestnut' (7.3%) were other preferred ingredients. The reasons for this preference were that they were 'healthy' by (47.1%) and had a 'good taste' by (36.6%). Evaluation of the recognition of functional bakery products revealed that, 'nutrition' had the highest scale of recognition of (3.80 out of 5), and followed by 'healthy' with (3.69 out of 5). Conversely, 'proper price' received a below average score of 2.97, indicating dissatisfaction. Moreover, the overall average satisfaction with functional bakery products was 3.37, and with the taste, nutrition, and quality receiving a highly satisfactory score of 3.52, and price receiving an unsatisfactory score of 3.00. The factors most important to improve functional bakery products were, 'variety' (37.6%) was most important, followed by 'increased nutrition' (21.2%) and 'lower price' needed to be improved as well. The factor that had the greatest influence on the decision to purchase functional bakery products was 'material' with (3.737) followed by 'name value' with (3.56).

• 한국작물학회지
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• 제47권3호
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• pp.143-146
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• 2002

조기재배 고구마에서 2모작 재배용 묘를 생산하기 위하여 조기재배의 수량감소를 최소화하고, 2모작용 묘를 최대 생산하기 위한 최적 지상부 경엽절제에 대한 시험 결과는 다음과 같았다. 1. 무절제와 비교한 지상부 및 지하부의 생체중과 건물중은 15% 경엽절제 이하에서 수확기에 동일수준이었다. 2. 경엽절제 정도가 많아짐에 따라 지상부 경엽중의 감소는 주만장과 주만절수의 감소가 원인이었다. 3. 총저중이 20% 경엽절제까지 같은 수준이었던 것에 대하여 50g 이상의 상저중은 15% 경엽절제까지 무절제와 비교하여 동일 수준이었던 것은 경엽절제 정도가 많아짐에 따라 50g 이하의 설저의 증가로 상품화율이 감소했기 때문이었다.

• 셀메드
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• 제7권3호
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• pp.13.1-13.10
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• 2017

Nutrition is one of the factors influencing cognitive functions. But, the role of nutrition on cognitive functions within the elderly is recognized to a lesser degree. The aim of this academic endeavor was to analyze the affiliation between nutritional status and cognitive functions of the elderly in Korea. 316 subjects, of 114 male and 201 female aged above 65 years, were gathered from university hospital clinics, Elderly Welfare Centers, and Health Welfare Centers located in the district of Seoul, Gyeonggi province, and Incheon in Korea. The cognitive function was tested by the Korean-Mini Mental State Examination (K-MMSE) questionnaire. Nutrition intake was assessed by a 24-hour recall, a food frequency questionnaire (FFQ). Nutrition adequacy and quality were assessed by the Nutrition Screening Initiative (NSI) checklist, the nutritional adequacy ratio (NAR), and the mean adequacy ratio (MAR). The elderly with normal cognitive function status displayed significantly higher levels of calcium, riboflavin, thiamin, folate, vitamin C, vitamin E, vitamin B6, phosphorous, potassium, iron, niacin, zinc, animal fat, animal protein, polyunsaturated fatty acids, total protein, vegetable oil, vegetable protein, fiber than levels of the elderly with moderate cognitive impairment. The K-MMSE score positively correlated with of high consumption of pork, white radish, sea mustard, tomato, tangerine, grape, apple, and ice cream, and K-MMSE was negatively associated with high intake of potato, anchovy, fish cake, and mushroom. The findings suggest that nutritional status is affiliated with cognitive function within the elderly of Korea. The consumption of variety of foods and nutrients ensures adequate cognitive function in the Korean elderly.

• Plant Biotechnology Reports
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• 제4권1호
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• pp.49-52
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• 2010

A DNA extraction method using Chelex 100 is widely used for bacteria, Chlamydomonas, and animal cell lines, but only rarely for plant materials due to the need for additional time-consuming and tedious steps. We have modified the Chelex 100 protocol and successfully developed a rapid and simple method of DNA extraction for efficient PCR-based detection of transgenes from a variety of transgenic plant and algal species. Our protocol consists of homogenizing plant tissue with a pestle, boiling the homogenized tissue in a microfuge tube with 5% Chelex 100 for 5 min, and centrifuging the boiled mixture. The supernatant, which is used for PCR analysis, was able to successfully amplify transgenes in transgenic tobacco, tomato, potato, Arabidopsis, rice, strawberry, Spirodela polyrhiza, Chlamydomonas, and Porphyra tenera. The entire DNA extraction procedure requires <15 min and is therefore comparable to that used for bacteria, Chlamydomonas, and animal cell lines.

• 한국균학회지
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• 제39권2호
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• pp.141-144
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• 2011

균류의 단기 보존은 일반적으로 한천이 포함된 고체 사면배지에서 이루어진다. 본 연구에서는 2~7년간 PDA 배지를 이용하여 $4^{\circ}C$에서 보관된 담자균류 28종 76개 균주들에 대한 활력을 조사하였다. 균의 생존율은 보존기간이 7년 82%, 6년 86%, 5년 94%, 4년 96%, 3년 94%, 2년 94%로 나타났다. 표고 균주의 배지 부피는 2년 이후부터 감소하였다. 보존된 배지의 pH는 2007년에 5.42였지만 보존기간이 길어질수록 거의 중성 pH에 도달하였다.

• 농업과학연구
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• 제43권3호
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• pp.432-440
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• 2016

The consumption of pre-treated vegetables (including fresh-cut vegetables) that are washed, peeled, and trimmed has been significantly increased because of their easy use for cooking. Vegetable cutting machines have been widely utilized for producing fresh-cut vegetables or agricultural products of different sizes; however, the design standard is not established for specific types of agricultural products. Therefore, this study was conducted to determine mechanical properties (compressive and shear forces) of targeted agricultural products (radish, carrot, squash, cucumber, shiitake mushroom, and sweet potato) for developing a multipurpose vegetable cutting machine. According to ASAE standard (s368.3), compressive and shear forces of targeted agricultural products were measured by using a custom built UTM (universal testing machine). Shape type of samples and speed ranges (5 - 15 mm/min) of loading rate on bioyield and shear points varied depending on the targeted agricultural product. The range of averaged bioyield points of targeted agricultural products were between 7.89 and 146.98 N. On the other hand, their averaged shear points ranged from 22.50 to 53.47 N. Results clearly showed that the bioyield and shear points of targeted agricultural products were thoroughly affected by their components. As measuring compressive and shear forces of a variety of agricultural products, it will be feasible to calculate blade cutting force for designing multipurpose vegetable cutting machine.

• 한국식생활문화학회지
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• 제24권5호
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• pp.533-539
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• 2009

Frying is the most popular cooking style used in food service institutions in Korea because fried dishes are well accepted by students. However, trans fat contents reduction have recently been required in many foods for health reasons Therefore, alternatives to frying such as oven baking or roasting are being used in many institutions. Steam convection ovens provide dry heat as well as steam so that they can be used to make a wide variety of Korean dishes. In this study, a menu acceptance test was conducted over 2 weeks. Pork, chicken, fish and potato dishes cooked by conventional cooking methods were served for 4 successive days, after which the same dishes were prepared using the oven. Overall, 322 junior high school students evaluated the traditionally cooked foods, while 316 evaluated the oven cooked foods. Comparison of the foods prepared using both methods only revealed a significant difference in the acceptability of foods on the fish menu (p<0.05). Specifically, the acceptance of fried fish was higher than that of the oven baked fish. Additionally, overall acceptance of the menu by males was higher than the acceptance by girls. Furthermore, students who had the preference for special ingredients showed a higher menu acceptance for the menu cooked with those ingredients. On average, approximately 25% of the meal was not consumed and left as plate waste. The portion of the fried fish not consumed was smaller than that of the oven cooked fish, but the portion not consumed did not vary based on cooking method for any other foods evaluated. Overall, it is expected that the oven cooking method will be a good substitute for frying or other cooking method for traditional Korean dishes.

• 한국자원식물학회지
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• 제10권1호
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• pp.30-38
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• 1997

국내의 감자 재배지역(栽培地域)에 알맞은 가공용(加工用) 감자품종(品種) 선납(選拉) 및 재배지역(栽培地域)간 품질성(品質性)에 관한 기초자료를 얻고자 재배지역(栽培地域)별로 유망(有望) 가공용(加工用) 품종(品種) 및 계통(系統)들의 규격서(規格薯) 수량(收量) 및 품질(品質)에 미치는 영향에 대하여 시험을 실시한 결과를 요약하면 다음과 같다. 지역에 따른 평 균 총서(總薯) 수량(收量)을 보면 대관령(大關嶺) (3,051 Kg/10a), 강릉(江陵(2,863 Kg/10a), 보성(寶城) (2,844Kg/10a)의 순이었으며 평균(平均) 규격서(規格薯) 수량(收量)은 대관령9大關嶺) (2,351 Kg/10a), 강릉(江陵) (2, 278 Kg/10a), 보성(寶性) (2,246Kg/10a)의 순으로 높았다. 품종별(品種別) 수량(收量)은 각지역 모두 Gemehip이 가장 많았으며 이는 식용(食用)으로 사용하기에 유망하였다. 비중(比重)은 대관령(大關嶺)지역이 비교적 높았으며 품종간(品種間)에는 대서(大西)와 NS1이 높았고 Gemchlp은 낮았으며, 클루코스 함량(含量)은 NS1, 대서(大西), NS2, 수미(秀美) 및 Gemchip순으로 낮게 나타났고 chip color 역시 NS1, 대서(大西), NS2, Gemchip 수미(秀美)순으로 밝아 글루코스 함량과 밀접한 관계를 보여주었다. 제품수율(製品收率)은 각 지역 모두 대서(大닌西), NS1, 수미(秀美), NS2, Gemchip 순(順)으로 나타나 비중(比重)의 순서와 일치하였다. 이상과 같은 결과로 볼 때 대서(大西)와 NS1 품종(品種)이 칩 가공(加工)용 유망품종(有望品種)으로 판단되었다.