• Journal of Animal Science and Technology
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• v.59 no.4
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• pp.7.1-7.13
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• 2017

Background: Selenium is one of the trace minerals whose deficiency is known to lead to complications of female reproduction. The identified gaps in researches regarding selenium and pregnancy include optimizing the dosage of selenium supplementation, timing of supplementation, finding the best form and type of selenium, and selenium administration combined with other antioxidants. Hence, this study was conceptualized to address one of the identified gaps, that is, to find out the best timing of selenium administration around the time of pregnancy. Specifically, this study aimed to assess the effects of maternal Selenium-supplementation, administered at various stages of periconception period, on murine blastocyst morphology, percent occurrence of good quality blastocysts, and implantation status. Methods: ICR female mice were randomly assigned into the unsupplemented group (Group I) receiving basal diet without selenium, and treatment groups given with $3.0{\mu}g$ selenium-supplement per day during pregestation only (Group II), pregestation-throughout-gestation (Group III) and gestation only (Group IV). Both blastocyst morphology and implantation status were assessed. Results: The morphometric measurements of blastocysts appeared to be unaffected by selenium-supplementation at different stages of periconception. Selenium-supplementation at pregestation only (Group II) and gestation only (Group IV) produced higher percent occurrence of good quality blastocysts and lower percent pre-implantation loss than Group III. Among all the treatment groups, Group III (Selenium-supplementation during pregestation-to-gestation) yielded the lowest quality blastocysts and highest percent pre-implantation loss. Conclusion: Maternal selenium-supplementation during pregestation and gestation stages of the periconception period yielded a high percent occurrence of good quality blastocysts and pre-implantation success.

• The Journal of Korean Medicine
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• v.26 no.2 s.62
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• pp.32-51
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• 2005

Objectives: To study the effect of Artemisiae Capillaris Herba extracts, that have been used as oriental medicine to treat liver disease, on the perinatal and lactational n;)productive toxicity of SD rats when administered by oral lavage. Methods: Female SD rats were dosed from 6 days of gestation to 3 weeks postpartum. This was conducted in accordance with the recommendations of the KFDA Guidelines for Detection of Toxicity to Reproduction for Medicinal Products. Results: No Artemisiae Capillaris Herba extracts treatment-related changes in clinical signs, mortalities, implantation number, dead fetus number, loss rate of fetus, number of live young, survival rate of fetus, sex ratio of live young, external anomalies, pregnancy periods, viability index, lactational index, survival rate of litters at 4 days after birth or delivery index were demonstrated in any dosed levels in this study. However, the body weight and gains, food consumption and absolute organ weights of brain, adrenal glands, liver, spleen, kidney, ovaries and heart were significantly increased in 2000 or 1000mg/kg-dosing groups and the relative organ Weights of adrenal glands were significantly increased in 2,000mg/kg-dosing groups. Therefore, it was concluded that this increase was natural according to growth. Also, no changes of gross findings, clinical signs, mortalities, body weight and gains, physical development results, necropsy findings, organ weight, faculty test, open filed test and water-filled simple T-maze test, copulation, fertility, pregnancy indices, body weight and gains during gestation periods, necropsy findings, corpora lutea number, implantation number, implantation rate, dead fetus number, post-implantation loss rate, live young, post-implantation survival rate, sex ratio of live young, external anomalies and individual body weights of live young were demonstrated in any dosed levels in this study. Conclusions: It is considered that the NOAEL (No-Observed-Adverse-Effect Level) for perinatal and lactational reproductive toxicity of Artemisiae Capillaris Herba extracts was up to 2000mg/kg/day because no changes of other perinatal and lactational reproductive indices were demonstrated.

• The Korea Journal of Herbology
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• v.26 no.1
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• pp.47-52
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• 2011

Objectives : The present study has been undertaken to investigate the effects of Leonuri Sibirici Herba and Pruni Persicae Semen on pregnant rats. Method : In this experiment, the pregnant rats were administered by water extracts of Leonuri Sibirici Herba and Pruni Persicae Semen. The levels of weights, ALT, AST, ALP, BUN, creatinine, progesterone, Na and K in serum and reproductive indices of the rats were measured after treatment. Results : 1. The levels of body weight gains were not significantly changed in comparison with Control group in Leonuri Sibirici Herba group and Pruni Persicae Semen group. 2. In the levels of reproductive indices of the rats, the number of corpora lutea, implantation, viable fetuses, pre-implantation loss, post-implantation loss, fetal weight and placental wight were not significantly changed in comparison with Control group in Leonuri Sibirici Herba group and Pruni Persicae Semen group. 3. The levels of BUN, creatine, ALT, AST and ALP were not significantly changed in comparison with Control group in Leonuri Sibirici Herba group and Pruni Persicae Semen group. 4. The level of progesterone was not significantly changed in comparison with Control group in Leonuri Sibirici Herba group and Pruni Persicae Semen group. 5. The levels of Na and K were not significantly changed in comparison with Control group in Leonuri Sibirici Herba group and Pruni Persicae Semen group. Conclusion : Reviewing these experimetal results, it appeared that Leonuri Sibirici Herba and Pruni Persicae Semen had not toxicity on pregnant rats.

• The Korea Journal of Herbology
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• v.28 no.3
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• pp.33-38
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• 2013

Objectives : The present study has been undertaken to investigate the effects of Carthami Semen and Jogyeongjongok-Tang on pregnant rats. Method : In this experiment, the pregnant rats were administered by water extracts of Carthami Semen and Jogyeongjongok-Tang. The levels of weights, ALT, AST, ALP, BUN, creatinine, progesterone, Na and K in serum and reproductive indices of the rats were measured after treatment. Results : The levels of body weight gains were not significantly changed in comparison with Control group in Carthami Semen group and Jogyeongjongok-Tang group. In the levels of reproductive indices of the rats, the number of corpora lutea, implantation and viable fetuses, post-implantation loss, fetal weight and placental wight were not significantly changed in comparison with Control group in Carthami Semen group and Jogyeongjongok-Tang group. But pre-implantation loss was significantly increased in comparison with Control group in Carthami Semen group. The levels of BUN, creatine, ALT, AST and ALP were not significantly changed in comparison with Control group in Carthami Semen group and Jogyeongjongok-Tang group. The level of progesterone was not significantly changed in comparison with Control group in Carthami Semen group and Jogyeongjongok-Tang group. The levels of Na and K were not significantly changed in comparison with Control group in Carthami Semen group and Jogyeongjongok-Tang group. Conclusion : Reviewing these experimetal results, it appeared that Carthami Semen and Jogyeongjongok-Tang had not toxicity on pregnant rats.

• Reproductive and Developmental Biology
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• v.33 no.4
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• pp.195-202
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• 2009

Abnormal development and fetal loss during the post-implantation period are key concerns in the production of cloned animals by somatic cell nuclear transfer (SCNT). We hypothesized that the problems in cloned porcine offspring derived from SCNT are related to interactions between the conceptus and the endometrial environment. In the present study, we investigated expression patterns in the formation of placenta-related genes (Cdx2 and GATA6) in whole in vivo normal porcine embryos (from single cell to blastocyst) and each tissue of a normal fetus at Days 25, 35 and 55 by quantitative mRNA expression analysis using real-time PCR. The expression of Cdx2 and GATA6 mRNA increased to around the blastocyst stage. These genes were gradually decreased from the peri-implantation to post-implantation stage. Moreover, we examined the expression patterns of Cdx2 and GATA6 in Day 35 normal and SCNT cloned fetuses by the same methods. And, the level of Cdx2 and GATA6 gene expression in the extraembryonic tissue of SCNT was significantly higher than that of control tissues. From the present results, it can be postulated that the aberrant expression of Cdx2 and GATA6 genes in the endometrial and extraembryonic tissues at pre- and peri-implantation stages may be closely related to the lower efficiency of animal cloning.

• The Journal of Korean Academy of Prosthodontics
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• v.59 no.3
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• pp.281-290
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• 2021

Purpose. The aim of this study is to evaluate bone change around the implant in patients who underwent alveolar ridge preservation for implantation in the posterior teeth using radiographic data measuring changes of mesial, distal crestal bone level according to post-implantation, post-final prosthesis delivery and follow-up periods. Materials and methods. In total, 36 implants were placed in 32 patients. The mesial and distal crestal bone level of all the areas where alveolar ridge preservation was performed uses panoramic images taken post-implantation, post-final prosthesis delivery, and follow-up period each was measured and evaluated as a vertical value. The following factors were analyzed: associations between changes of crestal bone level and factors (e.g., age, sex, systemic diseases, dentist, implant location, tooth, bone type, membrane). The statistical analysis was performed using the mean, standard deviation and independent t-test, paired t-test (P < .05). Results. Analysis of crestal bone level differences between periods shows statistically significant differences (P < .05). There was no statistically significant difference when the changes of crestal bone level between post-implantation, post-final prosthesis delivery and follow-up periods were correlated with each factors. Conclusion. After alveolar ridge preservation, bone around the implant remained stable during the maintenance period without being affected by the patient and surgical factors, and alveolar ridge preservation is considered a clinically usable procedure.

• Korean Journal of Oriental Medicine
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• v.16 no.2
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• pp.143-157
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• 2010

Objectives : The objective of this study was to access the effect of Artemisiae capillaris herba Extracts on the organogenetic reproductive toxicity of New Zealand White rabbits when administered by oral gavage. Methods : Gestational New Zealand White rabbits were dosed from 6 days of gestation to 18 days of gestation. It was conducted in accordance with the recommendations of the KFDA Guideline for Detection of Toxicity to Reproduction for Medicinal Products. In addition, serum AST, ALT, LDH and ALP levels were detected with their necropsy and histopathological observation Results: No significant changes of body weights, gains and food consumption were demonstrated in all dosed groups compared to those of vehicle control group except for 2,000mg/kg-dosing group, which showed significantly increase of body weight compared to that of vehicle control group. Normal gross findings were demonstrated in all tested groups. No significant changes of number of corpora lutea, implantation, implantation rate, number of fetal death, loss rate of post-implantation, number of live youngs at C-section, survival rate of post-implantation, number of male live youngs at C-section, number of female live youngs, sex ratio of live young, external anomalies of live youngs and body weight of live youngs were demonstrated in all dosed groups compared to those of vehicle control group. Although some visceral variations such as thymic reminant in the neck, dilation of renal pelvis, dilated ureter and malpositioned left common carotid artery, no significant changes were demonstrated in all dosed groups compared to that of vehicle control group. Some malformations and variation were demonstrated with retardations. However, no significant changes were demonstrated in all dosed groups compared to that of vehicle control group. In addition, similar ossification numbers were detected in sternebrae, metacarpals, metatarsals, phalanges, cervical vertebrae, and sacral and caudal vertebrae. No Artemisiae capillaris herba Extracts treatment-related changes of serum AST, ALT, LDH and ALP levels were demonstrated in all dosed levels in this study. Conclusions : We could conclude that oral administration of Artemisiae capillaris herba Extracts hasn't an influence on the organogenetic reproductive toxicity.

• The Journal of Korean Academy of Prosthodontics
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• v.50 no.3
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• pp.191-197
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• 2012

Esthetics is important in restoring maxillary anterior area. Alveolar bone resorption and loss of interdental papilla may be minimized by immediate implantation. Previous studies showed successful results with the immediate implantation in healthy extraction socket, while many of these studies objected the immediate implantation into extraction sites with periapical lesions. Recent studies, however, reported successful results of the immediate implantation into extraction sites with periapical lesions with careful debridement of extraction sockets and general medication of antibiotics prior to implantation. A 73-year-old female visited the department of Prosthodontics in ${\bigcirc}{\bigcirc}$ University Dental Hospital with the chief complaint of fallen post-core and crown on left maxillary incisor. Although the incisor was with vertical root fracture and periapical lesion, the immediate implantation following the extraction of tooth was planned. Thorough socket debridement, irrigation with chlorhexidine, and tetracycline soaking were followed by immediate implantation. The general medication of antibiotics (Moxicle Tab.$^{(R)}$, 375 mg) was prescribed before and after the surgery. Immediate provisional restoration was delivered two days after the surgery, and the definitive metal-ceramic restoration was placed about six months later after reproducing the emergence profile from the provisional restoration. This case presents satisfying result esthetically and functionally upto two years after the placement of prosthesis with the harmonious gingival line and no loss of marginal bone.

• Journal of Veterinary Clinics
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• v.25 no.5
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• pp.385-390
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• 2008

The purpose of this study was to compare the long term prognosis of Ahmed valve implantation and medical therapy in dogs with early primary glaucoma. The medical records of dogs with early primary glaucoma from the Veterinary Medical Teaching Hospital of Seoul National University between January 2005 and May 2008 were reviewed. Five dogs underwent Ahmed valve implantation and medical therapy was performed in six dogs. Breeds of the dogs included Shih Tzu(n = 7), American cocker spaniel(n = 2), and miniature poodle(n = 2). Adequate intraocular pressure(IOP) was better maintained in the Ahmed valve implantation group than in the medical treatment group throughout the follow-up period. Mean time for vision loss to develop after treatment in the Ahmed valve implantation group($198.0{\pm}115.4$ days) was significantly longer than that in medical treatment group($43.2{\pm}30.4$ days, p < 0.05). Post-operative complications included occlusion of the tube by fibrin in the anterior chamber (n = 1), fibrous capsule about the episcleral base of the device(n = 1), and dislocation of the valve body(n = 1). It is considered that Ahmed valve implantation is more effective strategy to control IOP and maintain vision than medical treatment in early glaucomatous dogs.

• Journal of Veterinary Clinics
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• v.15 no.1
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• pp.83-93
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• 1998

The purposes of the present study were to evaluate the effect of estrone on the prevention of implantation and pregnancy and the concentrations of estradiol and progesterone or sodium and potassium in the plasma or uterine fluid of pregnant rats injected a single dose of 0, 0.1, 0.4 or 1.0 mg on days 5, 9, or 13 of pregnancy. The body weight gains of pregnant rats were significantly decreased in administering estrone on day 5 of pregnancyi no effect obtained on days 9 or 13. Numbers of viable fetuses were significantly decreased and pre- and post-implantation loss increased by administering estrone 0.1, 0.4 or 1.0 mg and numbers of implantation sites significantly increased by administering estrone 0.1 mg but significantly decreased or not occurred by 0.4 or 1.0 mg on day 5 of pregnancy, respectively, but no effect obtained on days 9 or 13. Plasma estradiol concentrations of pregnant rats were significantly increased on 1, 2 or 18 days after treatment with estrone (1.4 or 1.0 mg and 3 days after 0.4 mg on day 5 of pregnancy, respectively. But plasma progesterone concentrations had no effect on the treatment with estrone. Plasma sodium contents were significantly increased on 1 day after treatment with estrone 0.1 or 1.0 mg and 3 days after 0.1 mg on day 5 of pregnancy, respectively, except potassium content were no detectable change. Sodium contents in uterine fluid were significantly increased on 1 day after treatment with estrone 1.0 mg and 3 days after 0.1, 0.4 or 1.0 mg on day 5 of pregnancy, respectively. And potassium contents were significantly increased on 1 or 2 days after treatments with estrone 0.1 mg but significantly decreased on 3 days after 0.4 or 1.0 mg on day 5 of pregnancy, respectively. It is suggested that the prevention of implantation and failure of pregnancy could be induced by treatment with estrone 0.4 mg on day 5 of pregnancy and might be interrelated with changes of plasma estradiol concentration and sodium and potassium contents in uterine fluid.