• Korean Journal of Veterinary Research
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• v.32 no.2
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• pp.207-210
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• 1992

Oral infection of colostrum-deprived, neonatal piglets with porcine enterovirus serotype 3 can result in hepatic lesions with a short incubation period. In the thin section of the affected liver, crystalline arrays of virus particles characteristic of picornavirus were identified in the sinusoid endothelial cells and Kupffer cells. There were also cytoplasmic aggregates of electron- dense, virus-like particles in the hepatocytes. These findings suggest that porcine enterovirus serotype 3 has hepatotropism as well as neurotropism.

• Korean Journal of Veterinary Research
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• v.28 no.1
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• pp.137-143
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• 1988

In the course of studying porcine enterovirus infection in piglets, the vascular endothelial cells in the CNS of colostrum-deprived piglets with polioence-phalomyelitis were investigated by electron microscope. The experimental piglets were orally infected with the porcine enterovirus serogroup 3 isolated in Korea and necropsied at 7 days postinoculation. Crystalline arrays of viral particles were found in the vascular endothelial cells of the spinal cord and cerebellum. Aggregates of immature viral particles were occasionally observed in the vascular endothelial cells in the meninges. The rough ER was deprived of ribosomes, irregularly dilated and associated with viral crystals, There were abundant cytoplasmic filaments, dilatation of perivascular space, perivascular cuffing, and the partial distruptions of endothelial cell membrane and basal lamina.

• Korean Journal of Veterinary Research
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• v.25 no.2
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• pp.103-112
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• 1985

A total of 1-0 colostrum-deprived pigs (1 or 2-day-old) and 6 pigs (35-day-old), which had been raised by natural maternal nursing, were used to study the pathogenicity of the porcine enteroviruses by the intracerebral and intramuscular routes of inoculation, which the enterovirus were isolated from the diseased pigs in Korea. The porcine enteroviruses produced an identical polioencephalomyelitis in colostrum-deprived pigs and 35-day-old pigs, which manifested clinical signs and histopathological changes. Clinically it was characterized by incoordination, rise in rectal temperature, ataxia, flaccid paralysis in all the experimental groups. Histopathologically, the lesions were present in both grey and white matter at all levels of central nervous system, though usually more severe in the grey matter. These changes were characterized by meningeal infiltration, degeneration of nerve cells, neuronophagia, diffuse and focal gliosis, glial nodules and perivascular lymphocytic infiltrations. Ganglionitis of the dorsal root ganglia was frequently observed. On the basis of the clinical and histopathological changes mentioned above, it was concluded that porcine enteroviruses isolated in Korea were pathogenic strains which could produce polioencephalomyelitis in pigs. The most severe Jisease was prcduced by the inoculation of both enterovirus and hog cholera vaccine in the 35-day-old pigs at a time when colostral immunity presumably was low. The porcine enterovirus infections seemed to be associated with certain stress factor such as hog cholera vaccine in or immediately following the weanling period.

• Korean Journal of Veterinary Service
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• v.14 no.2
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• pp.110-120
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• 1991

28 porcine enteroviruses were isolated from 86 pig-feces of 9 swine farms located in south region, Chung-buk, from March to September 1990. Physico-chemical properties and pathogenicity of isolates were investigated. Results obtained throughout experiments are summarized as follows. According to the age, weanlings(40-90 days), sucklings(10-30 days) and adult pigs(6 months over) showed the isolation rate of 67%. 8% and 4%, respectively. By physico-chemical tests, YD-90/22, YD-90/43 and YD-90/64 strains were found to be ether, chloroform and PH stable. Nucleic acid test suggests the virus to have a DNA genome. Most of the Isolates were not evident of hemagglutinin using erythrocytes from various mammalian & avian. 22 strains among the isolates were shown CPE type I and the remainders were CPE type II. 3 strains among isolates of CPE type I strains were neutralized with high titers to serotype 2 antiserum. In the study on virus growth curve in PK-l5 cells, YD-90/22, YD-90/43 and YD-90/64 strains showed the maximum infectivity titers($10^{6.0}-l0^{6.5} TCID({50}ml$) at 4days post inoculation(PI). When 30 day-old commercial piglets were inoculated only intraoral route with the YD-90/22 strain at $10^{6.0} TCID_{50}ml,$ piglets not showed the symptoms. But piglets inoculated by intramuscle route, intraoral and intramuscle route after pretreat with dexamethasone(2.5mg /kg) for 5 days were shown the symptoms of anorexia, diarrhea, pyrexia and ataxia at 4th-6th days PI. The viral reisolation in the virus-inoculated piglets was examined from feces. The viruses were recovered intermittently from 2nd to 16th day PI and at 4th-6th day PI, all piglets excreted viruses.

• Korean Journal of Veterinary Research
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• v.59 no.2
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• pp.89-96
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• 2019

Enterovirus species G (EV-G) is highly diverse, and is ubiquitous in pig populations, usually without diarrhea. The present study aimed to investigate the presence of novel EV-G recombinants with the torovirus papain-like cysteine protease (PLCP) in Jeju pig herds. EV-G1-PLCP mono-infections were most prevalent in diarrheic weaned piglets. The PLCP genes of the Jeju isolates varied in size and junction sequences, and were greatly heterogeneous, with 77.0-90.7% homology amongst all recombinants. Our results suggest that the exogenous PLCP gene has undergone continuous rapid mutation in the individual EV-G genomes following cross-order recombination, thereby causing clinical disease in swine.

• Korean Journal of Veterinary Research
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• v.26 no.2
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• pp.301-305
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• 1986

Enterovirus 감염증시(感染症時) dexamethasone이 미치는 영향(影響)을 알아보기 위하여 초유(初乳)를 먹지 않은 1일령(日齡) 자돈(仔豚) enterovirus를 경구감염(經口感染)시키고 dexamethasone을 근육주사(筋肉注射)한 후 임상(臨床) 및 병리조직학적(病理組織學的)으로 관찰(觀察)하였던 바 다음과 같은 결과(結果)를 얻었다. 임상적(臨床的)으로는 dexamethasone을 투여(投與)한 자돈(仔豚)에서 감염후(感染後) 3일(日)째 체온(體溫)의 상승(上昇), 4일(日)째 보행실조(步行失調) 그리고 8일(日)째 횡와(橫臥)한 반면(反面) enterovirus만을 감염(感染)시킨 대조군(對照群)은 감염후(感染後) 7일(日)째 체온(體溫)의 상승(上昇), 14일(日)째 보행실조(步行失調)가 확인(認定)되었다. 병리조직학적(病理組織學的) 소견(所見)으로는 중추신경계(中樞神經系) 전반(全般)에 걸쳐 수막하(髓膜下) 원형세포(圓形細胞)의 침윤(侵潤), 혈관주위(血管周圍) 원형세포(圓形細胞)의 침윤(侵潤), 미만성(彌漫性) 또는 한국성(限局性) gliosis, 신경세포(神經細胞)의 퇴행성(退行性) 변화(變化)가 관찰(觀察)되었고, 특히 대뇌(大腦) 제(第)3뇌실부(腦室部)와 중뇌수도(中腦水道), 제(第)4뇌실(腦室) 및 척수(脊髓)의 중심관(中心管) 주변부(周邊部)에서 glia 세포(細胞)의 침윤(侵潤)이 현저(顯著)하였으며, 병변(病變)의 정도(程度)는 enterovirus만을 감염(感染)시킨 대조군(對照群)에 비해 dexamethasone을 투여(投與)한 자돈(仔豚)에서 훨씬 심하게 나타난 점으로 보아 dexamethasone 투여(投與)는 자돈(仔豚)의 enterovirus 감염증(感染症)을 촉진(促進)시킬 수 있는 한 유인(誘因)으로 간주(看做)된다.

• Korean Journal of Veterinary Research
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• v.38 no.2
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• pp.336-344
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• 1998

Eight monoclonal antibodies(MAbs) against the transmissible gastroenteritis virus (TGEV) were produced and characterized. Four of the MAbs were produced against a reference TGEV, Purdue strain(P115) and the others were produced against the Korean vaccine virus, Pyungtaek strain. Only one MAb(5C8) produced against P115 had neutralizing activity and was found to be E2 protein-specific. The other seven MAbs(4E2, 4G8, 5H6, 1F8, 2C6, 5H5, and 3A6) had specificity of nucleocapsid protein and no neutralizing activity. All MAbs reacted with different strains of TGEV, but none of the MAbs was reactive with porcine enteropathogenic viruses such as rotavirus, epidemic diarrhea virus and enterovirus by fluorescence antibody(FA) test.

• Korean Journal of Veterinary Research
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• v.27 no.2
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• pp.223-226
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• 1987

Serological classification was carried out on 10 samples of PEV isolated from the feces, the milk and the brain of the diseased pigs in Korea by CFT using type specific antisera of PEV. Ten samples of PEV were classified into 2 serotypes which were serotype 3 and 8. PEV from the feces belonged to serotype 8 and those from the milk and the brain to serotype 3. The cross reaction of serotype 3 occurred especially with serotype 1. Talfan virus. It can be said that at least 2 serotypes of PEV have existed in Korea.