• The Korean Journal of Mycology
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• v.43 no.1
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• pp.26-32
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• 2015

In this study, a new cultivar of Lentinula edodes was bred from dikaryotic KFRI 490 and monokaryotic KFRI 536 by Di-mon method. The new breed was named "Poongnyunko" and physiological characteristics was investigated. Its optimal growing temperature was $25^{\circ}C$, and it had thin density. Its colony was colorless and did not form any epithelium. On the $7^{th}$ day of mycelial growth, "Poongnyunko" was 56.6 mm, and showed higher cellulase activity compared with "Sanlim 4-ho"(52.9 mm). On the $5^{th}$ day of mycelial growth, "Poongnyunko" was 58.0 mm, and showed higher laccase activity compared with "Sanlim 4-ho" (55.6 mm). Its pH at a substrate was 6.0. Before cultivation, the pH of sawdust substrate was 4.7-5.5 went down to 3.7-3.9 after 120-day cultivation. After 60 days of cultivation, the change of $CO_2$ was the greatest. During the cultivation, $CO_2$ concentration in a growing bag was 4.67% to 3.90%.

• The Korean Journal of Mycology
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• v.43 no.2
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• pp.104-111
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• 2015

In this study, a new strain of Lentinula edodes (Shiitake) Poongnyunko was bred from dikaryotic KFRI 490 and monokaryotic KFRI 536 by Di-mon crossing method. In this study, the productivity was conducted through bed-log and sawdust substrate cultivation. The Poongnyunko showed significant intensive production from summer to autumn. Optimum temperatures of fruit-body formation were $17{\sim}24^{\circ}C$. The total amount of fruit-body production during 4 years by bed-log cultivation was $22kg/m^3$, and total yield of fruit-body until the 3rd flush was 648 g/ 2 kg. In case of sawdust substrate cultivation, the mycelial incubation period was 110~130 days. The suitable temperature and relative humidity were $18{\pm}1^{\circ}C$ and $90{\pm}5%$ respectively.

• Journal of Mushroom
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• v.16 no.4
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• pp.272-278
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• 2018

Lignin degrading enzymes from Lentinula edodes have broad substrate specificities, and therefore can degrade a variety of recalcitrant compounds. In this study, the lignolytic biodegradation was investigated in five different L. edodes fungi (Chunbaegko, Sanjo 303ho, Poongnyunko, Baekhwahyang, and Soohyangko). The fungi were evaluated for their ability to decolorize Remazol Brilliant Blue R (RBBR) in malt extract broth medium. Sanjo 303ho, Poongnyunko, Baekhwahyang, and Soohyangko rapidly decolorized RBBR within 7 days. The activities of manganese peroxidase (MnP) and laccase were determined in the absence and presence of lignin. Poongnyunko displayed the highest ligninolytic activity on day 7 of incubation (2,809 U/mg and 2,230 U/mg for MnP and laccase, respectively).

• Journal of Mushroom
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• v.15 no.1
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• pp.31-37
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• 2017

In this study, we investigated the amino acids content of shiitake mushrooms grown in eight different media and under three different temperature conditions. The sawdust media were supplemented with 4 kinds of nutrients - rice bran, barley, bean curd refuse, and red ginseng refuse in an 8:2 ratio with or without 0.5% calcium chloride, and the fruiting temperature was set to low ($13-15^{\circ}C$), medium ($18-20^{\circ}C$), or high temperatures ($24-26^{\circ}C$). Seventeen amino acids were detected, and the content varied depending on the compositions of the media. The highest total amino acid content (209.7 mg/g) was measured in mushrooms from the media with red ginseng refuse, and the glutamic acid content was the highest (51.3 mg/g) in mushrooms from the media with barley. In terms of fruiting temperature, the amino acid content was the highest (38.2%) at medium temperature followed by the content at low (32.5%) and high temperature (29.3%). The amino acid composition of shiitake mushrooms was similar between the medium and low temperature conditions.

• Journal of Mushroom
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• v.19 no.4
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• pp.347-352
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• 2021

In this study, to select suitable shiitake strains in Jangheung region for domestic strains of Lentinula edodes for log cultivation, a total of five different Korean strains(Chunbaegko, Sanjo 303ho, Poongnyunko, Baekhwahyang, Soohyangko) and two Japanese strains (Mori 290, Moriyujiro) were selected and a cultivation test was conducted to select suitable strains. As a result of the yield, Sanjo 303ho produced 40.9 kg, which was superior to 39.2 kg for Mori 290 and 37.7 kg for Moriyujiro. As for the quality characteristics, it was confirmed that Baekhwahyang had a hemispherical shape, and high quality fruit bodies. In conclusion, Sanjo 303ho, which has excellent production, and Baekhwahyang, which is advantageous for producing high-quality shiitake, were selected in Jangheung region for domestic strains.

• The Korean Journal of Mycology
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• v.46 no.1
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• pp.51-57
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• 2018

We investigated the antioxidant activities of nine Lentinula edodes varieties cultivated on oak log. The total polyphenol content (TPC), total flavonoid content (TFC), and antioxidant properties of L. edodes (varieties: Dasanhyang, Chunbaegko, Poongnyunko, Soohyangko, Baekwhahyang, Chunjang 1ho, Chunjang 2ho, Sanlim 5ho, and Sanlim 7ho) were evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay and the ferric reducing antioxidant power (FRAP) assay. The TPC and TFC of mushroom extracts ranged from 5.10 to 10.88 mg gallic acid equivalent (GAE)/g and 0.76 to 5.78 mg quercetin equivalent (QE)/g, respectively. In addition, mushroom extracts exhibited DPPH radical scavenging activity of 24.22~90.41%, 2.0 mg/mL and FRAP of 0.056~0.231, 2.0 mg/mL. The Chunbaegko variety showed the highest TPC (10.88 mg GAE/g), TFC (5.78 mg QE/g), DPPH activity (38.19~90.11%, 0.2~2.0 mg/mL), and FRAP (0.075~0.231, 0.2~2.0 mg/mL).