• Title/Summary/Keyword: Pomeranian

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The Effects of Rotating Magnetic Field on Growth Rate, Cell Metabolic Activity and Biofilm Formation by Staphylococcus Aureus and Escherichia Coli

  • Fijalkowski, Karol;Nawrotek, Pawel;Struk, Magdalena;Kordas, Marian;Rakoczy, Rafal
    • Journal of Magnetics
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    • v.18 no.3
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    • pp.289-296
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    • 2013
  • This work presents results of the study which concerns the influence of the rotating magnetic field (RMF) on the growth rate, cell metabolic activity and ability to form biofilms by E. coli and S. aureus. Liquid cultures of the bacteria were exposed to the RMF (RMF frequency f = 1-50 Hz, RMF magnetic induction B = 22-34 mT, time of exposure t = 60 min, temperature of incubation $37^{\circ}C$). The present study indicate the exposition to the RMF, as compared to the unexposed controls causing an increase in the growth dynamics, cell metabolic activities and percentage of biofilm-forming bacteria, in both S. aureus and E. coli cultures. It was also found that the stimulating effects of the RMF exposition enhanced with its increasing frequencies and magnetic inductions.

Extremely High Mortality Rate after a Successful Gastrectomy for Cancer in Older Adults

  • Ciesielski, Maciej;Kruszewski, Wieslaw Janusz;Szajewski, Mariusz;Walczak, Jakub;Spychalska, Natalia;Szefel, Jaroslaw;Zielinski, Jacek
    • Journal of Gastric Cancer
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    • v.19 no.2
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    • pp.202-211
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    • 2019
  • Purpose: Poor physiological reserve for withstanding major cancer surgery in older adults is an important concern in the selection of patients for oncologic gastrectomy. The present study aimed to analyze mortality patterns among patients who underwent gastrectomy for cancer according to age groups. The primary outcomes of this study were early- and middleterm results: 30-day and 3-, 6-, 12-, and 36-month mortality rates. Materials and Methods: A retrospective review of 288 patients who underwent surgical resection for gastric cancer in two centers was carried out. Patients were stratified into four groups according to age: 29-50 years (group I, n=27), 51-65 years (group II, n=117), 66-75 years (group III, n=81), and 76-92 years (group IV, n=58). Statistical calculations focused on the differences in the survival rates between groups I and II as well as between groups II and IV. Results: The middle-aged patients (group II) had significantly better 3-year survival than either the youngest (group I) or the oldest patients (group IV). The 6-month mortality rates were 16.9% in group III and 29.3% in group IV. Two-thirds of the patients from groups III and IV who died between 2 and 6 months after surgery had an uneventful postoperative course. Conclusions: Age is an important prognostic factor of middle-term survival after gastrectomy for cancer. Geriatric assessment and better patient selection for major surgery for cancer are required to improve the outcome of gastrectomy for cancer in patients aged over 75 years.

The Effect of Rotating Magnetic Field on Enterotoxin Genes Expression in Staphylococcus Aureus Strains

  • Fijalkowski, Karol;Peitler, Dorota;Zywicka, Anna;Rakoczy, Rafal
    • Journal of Magnetics
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    • v.21 no.1
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    • pp.141-147
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    • 2016
  • Staphylococcus aureus cultures exposed to rotating magnetic field (RMF) were studied in order to analyse the possible induced changes in staphylococcal enterotoxin genes (se) expression. Liquid cultures of S. aureus strains carrying different se were exposed to the RMF of magnetic frequency 50 Hz and magnetic induction 34 mT for 10 h at $37^{\circ}C$. Three time points of bacterial growth cycle were considered for RNA extractions. Gene expression analyses were evaluated using real-time quantitative PCR method. The present study confirmed, that the RMF can stimulate the growth rate of S. aureus cultures in comparison to the unexposed controls, while the stimulation is not strain dependent. The studies have also shown, that the RMF, depending on the exposure time but regardless the bacterial strain, can influence on the expression of various se. In general, except for sea, as a result of bacterial exposure to the RMF through subsequent growth phases, the expression of se decreased, reaching the values below results recorded for unexposed controls. In the case of sea expression remained at a lower level as compared to the control, regardless the time of exposition.

MMP3 in Comparison to CA 125, HE4 and the ROMA Algorithm in Differentiation of Ovarian Tumors

  • Cymbaluk-Ploska, Aneta;Chudecka-Glaz, Anita;Surowiec, Anna;Pius-Sadowska, Ewa;Machalinski, Boguslaw;Menkiszak, Janusz
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.5
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    • pp.2597-2603
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    • 2016
  • Ovarian cancer is a highly malignant neoplasm with high mortality rates. Research to identify markers facilitating early detection has been pursued for many years. Currently, diagnosis is based on the CA 125 and HE4 markers, as well as the ROMA algorithm. The search continues for new proteins that meet the criteria of good markers A total of 90 patients were included in the present study, allocated into: group 1, ovarian cancer, with 29 patients; group 2, endometrial cysts, with 30s; and group 3, simple ovarian cysts, with 31. Following histopathological verification, the CA 125, HE4, and metalloproteinase 3 (MMP3) levels were determined and the ROMA algorithm was calculated for all patients. The mean concentrations of all determined proteins, CA 125, HE4, and MMP3, as well as the ROMA values, were significantly higher in group 1 (ovarian cancer) compared to group 3 (simple ovarian cysts). The highest significant differences for the CA 125 levels (p<0.000001) and ROMA (p<0.000001) values were observed in postmenopausal women. For HE4, statistical significance was at the level of p=0.00001 compared to p=0.002 for MMP3. For the differentiation between ovarian cancer and endometrial cysts, the respective AUC ratios were obtained for CA 125, HE4, and MMP3 levels, as well as the ROMA values ( 0,93 / 0,96 / 0,75 / 0,98). After removing the post-menopausal patients, the MMP3 AUC value for ovarian cancer vs. benign ovarian cysts increased to 0.814. For post-menopausal women, the MMP3 AUC value for ovarian cancer vs. endometrial cysts was 0.843. As suggested by the results above, both the CA 125 and HE4 markers, as well as the ROMA algorithm, meet the criteria of a good diagnostic test for ovarian cancer. MMP3 seems to meet the criteria of a good diagnostic test, particularly in postmenopausal women; however, it is not superior to the tests used to date.

Lactation milk yield prediction in primiparous cows on a farm using the seasonal auto-regressive integrated moving average model, nonlinear autoregressive exogenous artificial neural networks and Wood's model

  • Grzesiak, Wilhelm;Zaborski, Daniel;Szatkowska, Iwona;Krolaczyk, Katarzyna
    • Animal Bioscience
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    • v.34 no.4
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    • pp.770-782
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    • 2021
  • Objective: The aim of the present study was to compare the effectiveness of three approaches (the seasonal auto-regressive integrated moving average [SARIMA] model, the nonlinear autoregressive exogenous [NARX] artificial neural networks and Wood's model) to the prediction of milk yield during lactation. Methods: The dataset comprised monthly test-day records from 965 Polish Holstein-Friesian Black-and-White primiparous cows. The milk yields from cows in their first lactation (from 5 to 305 days in milk) were used. Each lactation was divided into ten lactation stages of approximately 30 days. Two age groups and four calving seasons were distinguished. The records collected between 2009 and 2015 were used for model fitting and those from 2016 for the verification of predictive performance. Results: No significant differences between the predicted and the real values were found. The predictions generated by SARIMA were slightly more accurate, although they did not differ significantly from those produced by the NARX and Wood's models. SARIMA had a slightly better performance, especially in the initial periods, whereas the NARX and Wood's models in the later ones. Conclusion: The use of SARIMA was more time-consuming than that of NARX and Wood's model. The application of the SARIMA, NARX and Wood's models (after their implementation in a user-friendly software) may allow farmers to estimate milk yield of cows that begin production for the first time.

Reduction of proteins and products of their hydrolysis in process of cleaning post-production herring (Clupea harengus) marinating brines by using membranes

  • Drost, Arkadiusz;Nedzarek, Arkadiusz;Torz, Agnieszka
    • Membrane and Water Treatment
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    • v.7 no.5
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    • pp.451-462
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    • 2016
  • The molecular weight of proteins and protein hydrolysis products (PHP) in the fractionated post-production marinating brines left after herring marination process was determined by the HPLC. The proteins and PHP retention was evaluated in the three-stage purification process with the usage of polypropylene bag ($25{\mu}m$) and ceramic membranes with the cut-off of 150 and 1 kDa. It was found that the process of marination contributes to high participation of compounds in the post-production marinating brines. Those are characterised by low molecular weight, formed as a result of protein hydrolysis. Each stage of the scavenging process was reducing the content of proteins and PHP. The lowest retention was observed in the stage at which a PP bag was used, while the highest in the UF process, with the usage of 150 kDa membrane. The total retention of proteins and PHP differed according to the type of post-production marinating brines and reached the level of 16-22%.

The influence of age, sex, and tooth type on the anatomical relationship between tooth roots and the mandibular canal

  • Pucilo, Michal;Pucilo, Aleksandra;Safranow, Krzysztof;Nowicka, Alicja
    • Imaging Science in Dentistry
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    • v.51 no.4
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    • pp.373-382
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    • 2021
  • Purpose: Cone-beam computed tomography (CBCT) reconstructions were analysed to elucidate factors affecting the anatomical relationship between tooth roots and the mandibular canal(MC). Materials and Methods: Images of 300 volumetric tomography scans of patients aged between 20 and 79 years old (167 women and 133 men) were analysed. The mean distances between 2,053 dental root apices and the internal border of the MC were obtained by measuring the horizontal and vertical distances on coronal CBCT images. The actual distance was then calculated mathematically with the Pythagorean formula. The statistical significance of differences between men and women was assessed using the Mann-Whitney test. Correlations with patient age were evaluated with the Spearman rank correlation coefficient. Results: The mean distances ranged from 2.17 mm, for single right third molar roots in women, to 8.02 mm for single left third molar mesiolingual roots in men. The mean distances measured for the mandibular right second molar mesial roots and the right second premolar roots were larger in men than in women. Age showed a significant positive correlation with the measured distances for mesial and distal roots of the first and second molar on both sides and the right third molar, mesiolingual roots of the left third molar, and single roots of the right third molar. Conclusion: The root-to-mandibular canal distance depended on age and the type of tooth. In 2 root types, this distance was impacted by sex.

Natural infection of Crenosoma vulpis (Nematoda: Crenosomatidae) in an urban Korean dog

  • Choi, Sungjin;Sim, Cheolho;Kim, Hyeon-Cheol;Choi, Ho-Jung;Park, Bae-Keun
    • Korean Journal of Veterinary Research
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    • v.54 no.2
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    • pp.127-129
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    • 2014
  • A male, 3.5 month old Pomeranian dog was diagnosed with a natural infection of Crenosoma (C.) vulpis in Daejeon, Korea. First stage larvae of C. vulpis were detected by fecal examination using the Baermann technique. Thoracic radiographs revealed mild, pervasive bronchial infiltration of the lung. Enumeration of larvae via the McMaster technique revealed 1,600 larvae per gram of feces. The dog was treated with mebendazole, and clinical symptoms were resolved 2 weeks post-treatment, as indicated by the subject presenting fecal tests negative for C. vulpis.

Clinical, Radiographic, Echocardiographic, Intraoperative Findings of Diaphragmatic Hernia in a Pomeranian Dog (포메라니안견에서 발생한 횡격막허니아의 임상학적, 방사선학적, 초음파학적 및 수술적 소견)

  • 정순욱;박수현;이충헌;신영규;정월순
    • Journal of Veterinary Clinics
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    • v.16 no.2
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    • pp.478-481
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    • 1999
  • A 8 years old male Pomeranian weighing 4 kg was referred because of coughing of 4 months' duration. Heart sounds and cardiac apex beat were showed more intense on the right side. On radiographic views, loss of normal line of the diaphragm, gas-containing intestines and stomach in thoracic cavity, and right displacement of heart were observed. Ultrasonography revealed that liver located adjacent to the heart. Although the dog died due to severe respiratory disorder in surgical procedure, in thoracic and abdominal surgery, a large defect was found in the left and right ventral muscular portion and left central tendon of the diaphragm, extending from the esophageal hiatus to rib. Left and right cranial lobe of liver, small intestines, stomach and spleen were herniated in the thoracic cavity. Because of the size and chronicity of the defect in the diaphragm, closure was impossible with an abdominal muscle graft.

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Balloon valvuloplasty for severe subaortic stenosis in a Pomeranian dog

  • Han, Sang-Woo;Lee, Chang-Min;Park, Hee-Myung
    • Korean Journal of Veterinary Research
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    • v.56 no.4
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    • pp.261-264
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    • 2016
  • A nine-month-old Pomeranian dog with exercise intolerance and syncope was presented. The dog was depressed with grade 4 systolic murmur on cardiac auscultation. Based on cardiac examination, the dog was diagnosed with severe subaortic stenosis with involvement of the anterior mitral valve. ${\beta}-blocker$ administration was initiated and clinical signs were improved, but not fully resolved. Balloon valvuloplasty was performed and the dog survived for nearly one year without clinical sign and the cardiac troponin I level was normalized. This case describes successful management of severe subaortic stenosis in a small breed dog through balloon valvuloplasty.