• 제목/요약/키워드: Polyethylene Glycol treatment

검색결과 151건 처리시간 0.027초

Polyethylene Glycol 처리에 의한 생쥐 2세포기배의 분할구 융합에 관한 연구 (Studies on the Polyethylene Glycol-induced Fusion of Two-cell Mouse Embryo Blastomeres)

  • 양부근
    • 한국가축번식학회지
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    • 제14권2호
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    • pp.133-140
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    • 1990
  • This study was conducted to develop a simple and efficient technique for fusing 2-cell mouse embryos to obtain tertraploid embryos. Various concentration of PEG and exposure times were compared in order to determine the best condition for fusion and subsequent of fused embryos. The results obtained were follows ; 1. The incidence of fusion induction treated with 40% PEG(70.8%) and 45%(62.7%) for 60 sec. exposure were higher than those of 40% and 45% PEG for 30 sec., 90 sec., or 120 sec. exposure group. Also, the highest incidence of fusion induction(76.9%) was achieved with 120 sec. exposure at 50% PEG concentration. 2. Fused embryos after PEG treatment were cleavaged 2-to 4-cell, 8-cell, morula and blastocyst at 20-24 hr., 30-34., 44-52 hr., respectively, and were not different from those obtained fleshly. 3. The high proportions of the embryos developed to blastocysts after blastomere fusion with 40% PEG for 60 sec., 45% PEG for 60 sec. and 50% for 120 sec. were 66.7%(42/63), 69.0%(29/42) and 32.0%(16/50), respectively, this trend indicated that the fusion rate was similar to the incidence of fused embryos forming blastocysts. 4. The cell number of blastocyst developed from fused embryos(18.7 2.6) was samller than that of untreated embryos(48.9 1.69)

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PEG 포괄고정화담체를 이용한 난분해성 염색폐수 처리 (Refractory Textile Wastewater Treatment Using Cell-Immobilized Polyethylene glycol Media)

  • 한덕규;조영진;배우근;황병호;이용우
    • 대한환경공학회지
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    • 제28권3호
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    • pp.345-350
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    • 2006
  • 본 연구에서는 생물학적 처리방법으로 염색폐수의 난분해성 유기물질을 효과적으로 처리하기 위한 방안으로 활성슬러지를 Polyethylene glycol(PEG)에 포괄고정화한 담체를 유동상 반응기를 이용하여 난분해성 유기물질의 처리효율을 평가하고 적정 운전인자를 검토하였다. 담체는 내경 4 mm${\times}$높이 4 mm의 원통형으로 미세공극을 가진 PEG재질의 담체를 제조하기 위해 PEG-prepolymer와 미생물을 젤 상태에서 혼합하여 고형화 시켰으며, 이때 담체에 사용된 미생물은 염색폐수에 순응시키지 않은 상태에서 합성되었다. 연속 호기성 유동상 반응기의 유입수는 $SCOD_{Cr}$ 약 330 mg/L, $SBOD_5$ 20 mg/L 이하의 생물학적 처리수를 사용하였고, 체류시간의 변화에 따른 각 반응기의 난분해성 유기물질 제거효율을 비교하였다 생물학적 처리수를 유입수로 하여 동일한 HRT로 운전한 결과 모든 조건에서 45% 이상의 유기물 제거효율을 보였으며, HRT 12 hr이 유기물 제거효율 등을 고려할 때 안정된 효율을 얻을 수 있는 적정 체류시간으로 판단되었다. 특히 기질환경의 변화에 따른 유기물 제거효율의 변화를 확인하기 위하여 생물학적 처리전의 처리장 유입폐수를 처리한 결과 $SCOD_{Cr}$ 70% 이상, $SBOD_5$ 97% 이상의 높은 처리효과를 나타내었다. 포괄고정화 담체를 이용한 염색폐수처리에서 유동상 반응기는 기존 활성슬러지 공정을 대신하거나 혼용하여 적용하는 것이 가능할 것으로 판단되어진다.

중공사형 한외여과 막분리 공정에 의한 하천수 처리 (Lake Water Treatment Using a Ultrafiltration Membrane Process of Hollow Fiber Type)

  • 박진용
    • 멤브레인
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    • 제7권1호
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    • pp.39-47
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    • 1997
  • 자체적으로 설계한 막분리 장치 시스템을 사용하여 먼저 순수한 물(3차 처리수)을 대상으로 polysulfone 재질의 중공사형 한외여과막에 대한 분리 성능을 조사하였다. 분획분자량(Molecular cutoff) 5,000 및 10,000 두 종류의 한외여과막에 대하여 실험한 결과, 온도가 증가함에 따라 공급량에 대한 투과량의 비인 회수율이 증가하는 경향을 보였다. 또한, 다양한 분자량의 polyethylene glycol 및 dextran 2,000 ppm 수용액으로 분획분자량을 확인한 결과, 표시된 값보다 다소 큰 값을 얻을 수 있었다. 이러한 기초 실험결과를 토대로 하여, 최종적으로 생활하수의 유입으로 수질이 악화되고 있는 춘천시 공지천의 물을 처리 대상으로 선정하여 원수의 성분을 분석하고, 한외여과 실험을 실시하였다. 그 결과, 생물학적 산소요구량(BOD) 및 총고형물(TS), 탁도가 모두 처리수에서 원수보다 탁월한 감소를 나타내었다. 따라서, 중공사형 한외여과막을 사용한 생활하수 처리에 가능성을 본 연구를 통하여 확인할 수 있었다.

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Polyethylene Glycol 처리한 아크릴 운동용 양말의 축열 . 방열성과 수분전달 특성이 착용 성능에 미치는 영향 (The Effect of Thermal Storage/Release and Moisture Transport Properties of Polyethylene Glycol-Treated Acrylic Athletic Socks on the Wear Performance)

  • 조길수;이은주
    • 한국의류학회지
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    • 제19권1호
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    • pp.36-50
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    • 1995
  • The purpose of this study was 1) to estimate the improvement of thermal storage/release and moisture transport properties of PEG-treated acrylic athletic socks and suggest the optimum add-on for PEG treatment, 2) to investigate wear performance of untreated cocks and two kinds of socks treated with PEG of minimum and optimum add-on respectively, and 3) to consider the effect of thermal storage/release and moisture transport properties of PEG- treated socks on the wear performance and the subjective comfort zone. Thermal activities of specimens treated by PDC were evaluated on a DSC by measuring the heat of fusion on heating and the heat of crystallization on cooling. Moisture regain, absorption speed, wickability, water retenti on value, and water-vapor permeability were measured. In the wear trials that the subjects performed a subsequent exercise protocol wearing three differently treated socks in a conditioned environment ($14\pm2^{\circ}C$, 65$\pm$2% R.H.), microclimate temperature and humidity, and subjective wear sensations including thermal sensation, wettedness, softness, fit, and overall comfort were obtained. PEC-treated specimens with more than 20% add-on showed thermal storage on heating and thermal release on cooling by a DSC and the heat contents of treated ones were generally proportional to the add-ons. Moisture transport properties were highly improved after PEG treatment and increased rapidly with increasing add-on. The tendencies were, however, relaxed above 50% add-on and the treated knits were much stiffer above that add-on. In the wear trials of untreated, PEG add-on 20%, and 50% acrylic socks, the changes of microclimate temperature of 50% socks were significantly less than that of 20% socks. PEG add-on 50% socks showed significantly less changes of microclimate humidity than other two kinds of socks. Three kinds of socks showed significant differences in overall comfort and add-on 50% socks were accepted more comfortable than other two kinds of socks. Comfort zone of foot was extended after PEG treatment on socks and it implied that the subjects wearing PEG- treated socks felt comfortable in wider ranges of microclimate temperature and humidity.

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폴리에틸렌글리콜(PEG) 함침처리한 완도선 목재의 표면 안정화를 위한 하이드록시프로필 셀룰로오스(HPC) 처리효과 (Effect of Hydroxypropyl Cellulose Treatment for Surface Stabilization of Waterlogged Wood of Wan-do Shipwreck Impregnated with Polyethylene Glycol)

  • 김응호;한규성
    • 보존과학회지
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    • 제32권2호
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    • pp.155-165
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    • 2016
  • 수침고목재인 목재유물의 보존처리에 가장 많이 사용되는 PEG 함침 후 진공동결건조 방법의 단점인 표면 결함에 대한 안정화처리를 위해 HPC를 도포하였다. HPC의 효과를 확인하기 위해서 외관 및 실체현미경 관찰, 색차, 중량변화율, 치수변화율을 측정하고, 통계분석을 실시하였다. 목재시료의 표면에 HPC가 얇은 층을 형성하였고, 가도관의 관공과 균열의 틈 사이를 메우고 있었다. HPC 1,000,000을 처리한 시료의 부후되지 않은 표면에서 나타난 백화현상을 제외하고 다른 변화는 나타나지 않았다. 열화시킨 시료는 수분 흡착에 의한 색변화가 나타났다. 시료 표면의 얇은 HPC 층은 열화 후에도 유지되고 있었으며, 수분 흡착에 의한 중량변화와 치수변화는 HPC에 의해 억제되는 것으로 나타났다.

Purification of a Thermostable Recombinant Sulfolobus solfataricus Esterase Expressed in a Mesophilic Host

  • 김성훈;이선복
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2000년도 춘계학술발표대회
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    • pp.501-504
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    • 2000
  • The purification of a thermostable esterase expressed in Escherichia coli was investigated using thermoprecipitation of unclarified cell homogenates followed by after applying the heat-treated lysate to phenyl-sepharose column, and elution with detergent. Heat treatment at $70^{cdot}C$ was capable of removing to E. coli proteins. Specially, the thermoprecipitation with 15% polyethylene glycol 8000 can remove host proteins and nucleic acids efficiently. Various detergents were used to recover the esterase, which was strongly bound to phenyl-sepharose resin. Triton X-100, non-ionic detergent, was found to be the most efficient of all tested detergents.

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The influence of polymer compounds on the HTS films

  • Soh, Deawha;Korobova, N.;Park, Jung-Cheul;Jeun, Yong-Woo
    • 한국항해항만학회:학술대회논문집
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    • 한국항해항만학회 2000년도 추계학술대회논문집
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    • pp.112-115
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    • 2000
  • In this work the results of the systematic investigations on the effect of organic addition by using polymer compound as starting materials on the superconducting properties of YBCO electrophoretic deposited films on silver substrate are presented. The characteristics of the films were examined by X-ray diffraction and SEM observation. Our results show that the adhesion and microstructure of these films are sensitive to the nature of polymer compounds and sintering conditions (electrophoretic deposition, drying and heat-treatment procedures). To develop optimum microstructures for samples processed in this manner it is necessary to have an understanding of how these processes affect the microstructure and hence the properties of ceramic superconductors.

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The influence of polymer compounds on the HTS films

  • Soh, Deaw-Ha;Korobova, N.;Park, Jung-Cheul;Jeon, Yong-Woo
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2000년도 추계학술대회 논문집
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    • pp.112-115
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    • 2000
  • In this work the results of the systematic investigations on the effect of organic addition by using polymer compound as starting materials on the superconducting properties of YBCO electrophoretic deposited films on silver substrate are presented. The characteristics of the films were examined by X-ray diffraction and SEM observation. Our results show that the adhesion and microstructure of these films are sensitive to the nature of polymer compounds and sintering conditions (electrophoretic deposition, drying and heat-treatment procedures). To develop optimum microstructures for samples processed in this manner it is necessary to have an understanding of how these processes affect the microstructure and hence the properties of ceramic superconductors.

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Efficacy and Safety of Combined Oral and Enema Therapy Using Polyethylene Glycol 3350-Electrolyte for Disimpaction in Pediatric Constipation

  • Yoo, Taeyeon;Bae, Sun Hwan
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • 제20권4호
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    • pp.244-251
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    • 2017
  • Purpose: We evaluated the efficacy and safety of combined oral and enema therapy using polyethylene glycol (PEG) 3350 with electrolyte solution for disimpaction in hospitalized children. Methods: We retrospectively studied 28 children having functional constipation who received inpatient treatment between 2008 and 2016. The amount of oral PEG 3350 electrolyte solution administered was 50-70 mL/kg/d (PEG 3350, 3-4.1 g/kg/d), and an enema solution was administered 1-2 times a day as a single dose of 15-25 mL/kg (PEG 3350, 0.975-1.625 g/kg/d). A colon transit time (CTT) test based on the Metcalf protocol was performed in some patients. Results: Administration of oral and enema doses of PEG 3350 electrolyte solution showed $2.1{\pm}0.3$ times and $2.9{\pm}0.4$ times, respectively. After disimpaction, the frequency of defecation increased from $2.2{\pm}0.3$ per week to once a day ($1.1{\pm}0.1$ per day). The number of patients who complained of abdominal pain was reduced from 15 (53.6%) to 4 (14.3%). Before hospitalization, nine patients underwent a CTT test, and 5 of 9 patients (55.6%) were classified as belonging to a group showing abnormalities. And in some patients, mild adverse effects were noted. We examined electrolytes and osmolality before and after disimpaction in 16 of 28 patients, and no abnormalities were noted. Conclusion: In terms of therapeutic efficacy and safety, combined oral and enema therapy using high-dose PEG 3350 with electrolytes is considered superior to conventional oral monotherapy or combined oral and enema therapy on an outpatient basis.

Dimensional Stability of Bentwoods by Treatment Conditions

  • Jung, In-Suk;Lee, Weon-Hee;Chang, Jun-Pok;Bae, Hyun-Mi
    • Journal of the Korean Wood Science and Technology
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    • 제30권3호
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    • pp.85-90
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    • 2002
  • This study was carried out to investigate the dimensional stability of bentwoods by three treatments: steaming, urethane varnish coating, and polyethylene glycol (PEG) treatment. Bentwood processing employed a bending-jig with only 4 cm radius of curvature (ROC). The used species were bitter wood (Picrasma quassioides), painted maple (Acer mono), and birch (Betula schmidtiii). The bending properties of these are well-known in bentwood production (Jung et al., 2002). The bentwoods were treated repeated at room temperature [20℃, RH 80% (12 hours) and 40℃ under RH 10% (12 hours)]. To estimate the dimensional stability of bentwoods, we measured the radius of curvature and end-distance. The best results could be attained with PEG treatment. Steaming was the worst treatment. Comparing the properties of the different species, the dimensional stability of bitter wood was excellent. It was concluded that the steaming treatment was unsuitable for dimensional stability of bentwoods.