• 대한기계학회논문집A
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• 제27권10호
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• pp.1644-1652
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• 2003

The monitor product is packed by cushioning materials because the monitor can be broken during transportation. However, the addition of the cushioning material increased the volume of the product. Therefore, it is required that the usage of cushioning material be minimized. In practice, design engineers have followed the ad hoc design with experiences of predecessors. Automation of the design process is very important for the reduction of engineering cost, and can be achieved by an excellent design process and software development. According to Axiomatic design, a design flow is defined and a software system is developed for automated design. At first, a basic model is defined. A user can modify the model from menus and design is carried out according to the input from the user. Finite element models are automatically generated based on the design. A nonlinear finite element analysis program called LS/DYNA3D is linked for the impact analysis. The process of Design of Experiments using orthogonal array is installed to minimize the maximum acceleration in drop test. Therefore, a new design can be proposed by the system. The program is designed according to the Independence Axiom of Axiomatic design. FRs and DPs of the software system are defined and decomposed by zigzagging process. Independent modules can be generated by analysis of the full design matrix and each module is coded as class in Object Oriented Programming (OOP). Design results are discussed.

• Natural Product Sciences
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• 제18권1호
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• pp.16-21
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• 2012

Honokiol, a naturally occurring neolignan mainly found in Magnolia species, has been shown to have the anti-angiogenic, anti-invasive and cancer chemopreventive activities, but the molecular mechanism of actions has not been fully elucidated yet. In the present study, we investigated the effect of honokiol on the growth inhibitory activity in cultured SNU-638 human gastric cancer cells. We found that honokiol exerted potent antiproliferative activity against SNU-638 cells. Honokiol also arrested the cell cycle progression at the G0/G1 phase and induced the apoptotic cell death in a concentration-dependent manner. The cell cycle arrest was well correlated with the downregulation of Rb, cyclin D1, cyclin A, cyclin E, and CDK4 expression, and the induction of cyclin-dependent kinase inhibitor p27. The increase of sub-G1 peak by honokiol was closely related to the induction of apoptosis, which was evidenced by the induction of DNA fragmentation, the cleavage of poly(ADPribose) polymerase, and the sequential activation of caspase cascade. These findings suggest the cell cycle arrest and induction of apoptosis might be one possible mechanism of actions for the anti-proliferative activity of honokiol in human gastric cancer cell.

• 한국잡초학회지
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• 제32권3호
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• pp.230-239
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• 2012

Coconut (Cocos nucifera L) is one of the predominant plantation crops in Sri Lankan economy which is known to have existed for over thousands of years. During the past decades coconut production had been reduced by a significant quantity. The usage of poor quality planting materials is a major reason for the low coconut production. Thus much attention needs to be paid in coconut nurseries. Weed management is a critical management practice in the nursery. Though glyphosate application is becoming popular in nurseries it can affect weeds as well as coconut seedlings growth. Therefore the effects of glyphosate were evaluated by determining the growth of shoot and root of coconut seedlings. Poly bag nursery was prepared and three treatments were used. Treatments were no glyphosate and manual weeding ($T_1$), application of glyphosate 1.08 ai kg $ha^{-1}$ at 2 monthly interval ($T_2$) and application of glyphosate 1.44 ai kg $ha^{-1}$ at 2 monthly interval ($T_3$). Application of glyphosate at early stage of seedling growth had a no significant effect on growth parameters tested. However, the concentrations of glyphosate negatively affected numbers, volumes and dry weights of secondary, tertiary and quaternary roots at the latter stage of seedling growth. The leaf area and the height of seedling were significantly reduced by the highest concentration of glyphosate. Among the growth parameters tested, seedling girth and shoot dry weight were not affected by the application of glyphosate. These results revealed that the usage of glyphosate at both concentrations negatively affected root growth of coconut seedlings. Based on these results, the both concentration levels of glyphosate should be applied to coconut nurseries before sprouting the seed nuts.

• International Journal of Oral Biology
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• 제43권2호
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• pp.61-68
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• 2018

Codium fragile (Suringar) Hariot is an edible green seaweed that belong to the Codiaceae family and has been used in Oriental medicine for the treatment of enterobiasis, dropsy, and dysuria. Methanol extract of codium fragile has anti-oxidant, anti-inflammatory and anti-cancer properties, although the anti-cancer effect on oral cancer has not yet been reported. In this study, we investigated the anti-cancer activity and the mechanism of cell death by methanol extracts of Codium fragile (MeCF) on human FaDu hypopharyngeal squamous carcinoma cells. Our data showed that MeCF inhibits cell viability in a dose-dependent manner, and markedly induced apoptosis, as determined by the MTT assay, Live/Dead assay, and DAPI stain. In addition, MeCF induced the proteolytic cleavage of procaspase -3, -7, -9 and poly(ADP-ribose) polymerase(PARP), and upregulated or downregulated the expression of mitochondrial-apoptosis factor, Bax(pro-apoptotic factor), and Bcl-2(anti-apoptotic factor). Futhermore, MeCF induced a cell cycle arrest at the G1/S phase through suppressing the expression of the cell cycle cascade proteins, p21, CDK4, CyclinD1, and phospho-Rb. Taken together, these results indicated that MeCF inhibits cell growth, and this inhibition is mediated by caspase- and mitochondrial-dependent apoptotic pathways through cell cycle arrest at the G1/S phase in human FaDu hypopharyngeal squamous carcinoma cells. Therefore, methanol extracts of Codium fragile can be provided as a novel chemotherapeutic drug due to its growth inhibition effects and induction of apoptosis in human oral cancer cells.

• 생약학회지
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• 제49권3호
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• pp.231-239
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• 2018

Parkinson's disease (PD), one of common neurodegenerative diseases, is caused by the death of dopaminergic neurons in the substantia nigra pars compacta. The loss of dopaminergic neurons in PD is associated with oxidative stress and mitochondrial dysfunction. Hyperoside (quercetin 3-O-${\beta}$-D-galactopyranoside) was reported to have protective properties against oxidative stress by reducing intracellular reactive oxygen species (ROS) and increasing antioxidant enzyme activity. In this study, we examined the neuroprotective effect of hyperoside against 1-methyl-4-phenyl pyridinium ($MPP^+$)-induced cell model of PD and the underlying molecular mechanisms. Hyperoside significantly decreased $MPP^+$-induced cell death, accompanied by a reduction in poly ADP-ribose polymerase (PARP) cleavage. Furthermore, it attenuated $MPP^+$-induced intracellular ROS and disruption of mitochondrial membrane potential (MMP), with the reduction of Bax/Bcl-2 ratio. Moreover, hyperoside significantly increased the phosphorylation of Akt, but it has no effects on $GSK3{\beta}$ and MAPKs. Pharmacological inhibitor of PI3K/Akt abolished the cytoprotective effects of hyperoside against $MPP^+$. Taken together, these results demonstrate that hyperoside significantly attenuates $MPP^+$-induced neurotoxicity through PI3K/Akt signaling pathways in SH-SY5Y cells. Our findings suggest that hyperoside might be one of the potential candidates for the treatment of PD.

• 동의생리병리학회지
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• 제18권1호
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• pp.101-109
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• 2004

Spatholobus suberectus belonging the family Leguminosae has been used for promoting blood circulation, removing blood stasis, tonifying the blood, relaxing tendons, stopping internal bleeding and eliminating dampness in oriental traditional medicine. This study investigates whether the water extracts of S. suberectus induce apoptotic cell death in Jurkat T-acute lymphoblastic leukemia (ALL) cells. Jurkat cells were increased inhibitions of cell viability in a concentration-dependent manner by S. suberectus, as measured by cell morphology. The capability of S. suberectus to induce apoptosis was associated with proteolytic cleavage of specific target protein such as poly (ADP­ribose)polymerase protein suggesting the possible involvement of caspases. The purpose of the present study is also to investigate the effect of S. suberectus on cell cycle progression. G1 checkpoin related gene products tested (cyclin D1, cyclin dependent kinase 4, retinoblastoma, E2Fl) were decreased in their protein levels in a dose-dependent manners after treatment of the extract. These results indicate that the increase of apoptotic cell death by S. suberectus may be due to the inhibition of cell cycle progression in wild type p53-lacking Jurkat cells.

• 동의생리병리학회지
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• 제19권6호
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• pp.1563-1567
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• 2005

Mori Cortex Radicis is distributed in Northwestern China, northern Asia, northern Europe, North America, and Korea. This extracts drops sugar in bloods and inhibits cyclic AMP phophodiesterase. In this study, we investigated whether Mori Cortex Radicis would cause apoptotic death of A549 lung cancer cells. To examine the apoptotic effect of Mori Cortex Radicis, cytotoxicity assay, DNA fragmentation analysis, caspase-3 activity assay, and Western blotting for caspase-3, caspase-9 and poly(ADP-ribose) polymerase (PARP) and cytochrome c were performed. Treatment of cells with Mori Cortex Radicis was shown to induce cell death in a dose-dependent manner. DNA fragmentation was made in response to Mori Cortex Radicis. The active fragments of caspase-3, caspase-9 and PARP were almost completely induced and cytochrome c was released following exposure to Mori Cortex Radicis. To elucidate the apoptotic mechanisms, RT-PCR and Western blot analyses for the expression of Bcl-2, Bu and Cox-2 were carried out. Treatment with Mori Cortex Radicis was expressed the reduction of Bcl-2 and Cox-2 and the induction of Bax. Especially p21 and p53 were increased prior to untreated control, while cyclin E and cyclin D1 decreased in the cytosol. These results suggest that the effect Mori Cortex Radicis is associated with the cell cycle arrest and pro-apoptotic cell death in A549 lung cancer cells.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2011년도 추계학술대회 초록집
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• pp.153-153
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• 2011

신 재생에너지 정책이 발전차액지원제도(Feed In Tariff; FIT)에서 신 재생에너지 의무할당제(Renewable Portfolio Standard; RPS)로 변화하면서 원별 경쟁이 가속화되고 있다. 태양광 산업 역시 이러한 환경 변화에 대처해야 하며, 그를 위한 정책 구성이 필요하다. 이에 태양광 산업 정책은 크게 두 부분으로 구분하여 진행해야 한다. 첫째, 폴리실리콘(Poly silicon)을 활용하는 다결정실리콘 태양광에너지에 초점을 맞춘 정책이며, 둘째, 미래에 상용화될 차세대 태양광 에너지에 대한 대비를 위한 연구 개발(R&D) 정책이다. 먼저 다결정실리콘 태양광에너지에 초점에서의 산업 정책은 산업육성정책과 수출정책, 인프라 구성 등으로 나눌 수 있다. 현재 과도한 국가부채로 인한 세계경제 악화로 태양광에너지 업체들의 경제성이 악화하고 있다. 더욱 빨리 그리드 패리티(Grid Parity)를 달성하기 위해 수직통합 등의 필요성이 대두하고 있다. 이에 본 연구는 그리드 패리티 달성시기를 위해 태양광 산업 내 세대 변화를 하는 경우와 하지 않는 경우를 비교하기 위해 고려할 요소를 분석하였다. 현재 신 재생에너지 가운데 태양광에너지는 타에너지원 대비 가격경쟁력을 갖추지 못한 상황이다. 그러나 수출을 고려했을 때의 향후 한국의 차세대 성장동력으로의 발전가능성이 존재한다. 따라서 가격경쟁력이 가장 중요한 영향을 미칠 신 재생에너지 의무할당제 정책 하에서 태양광에너지가 전혀 채택되지 않는 상황을 막기 위한 정책이 필요하다. 그를 위해 필요한 정책적 요소들을 알아보았다. 마지막으로 인프라 구성을 위해 태양광 산업의 가치사슬(Value Chain) 상에서의 기업 분포와 경쟁력에 대한 조사를 시행하였다. 이는 태양광 산업 내의 경쟁력을 갖춘 부문과 그렇지 못한 부문을 구별하기 위함이다. 미래에 상용화될 차세대 태양광 에너지를 준비하는 과정에서는 연구개발 관련 정책이 가장 중요하게 다뤄야 할 부분이며, 그를 위해 정부 차원에서 지원하고자 하는 기술로드맵 등에 대해서 정리하였다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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• pp.154-154
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• 2012

Flexible complementary inverters based on thin-film transistors (TFTs) are important because they have low power consumption and high voltage gain compared to single type circuits. We have manufactured flexible complementary inverters using pentacene and amorphous indium gallium zinc oxide (IGZO) for the p-channel and n-channel, respectively. The circuits were fabricated on polyimide (PI) substrate. Firstly, a thin poly-4-vinyl phenol (PVP) layer was spin coated on PI substrate to make a smooth surface with rms surface roughness of 0.3 nm, which was required to grow high quality IGZO layers. Then, Ni gate electrode was deposited on the PVP layer by e-beam evaporator. 400-nm-thick PVP and 20-nm-thick ALD Al2O3 dielectric was deposited in sequence as a double gate dielectric layer for high flexibility and low leakage current. Then, IGZO and pentacene semiconductor layers were deposited by rf sputter and thermal evaporator, respectively, using shadow masks. Finally, Al and Au source/drain electrodes of 70 nm were respectively deposited on each semiconductor layer using shadow masks by thermal evaporator. The characteristics of TFTs and inverters were evaluated at different bending radii. The applied strain led to change in voltage transfer characteristics of complementary inverters as well as source-drain saturation current, field effect mobility and threshold voltage of TFTs. The switching threshold voltage of fabricated inverters was decreased with increasing bending radius, which is related to change in parameters of TFTs. Throughout the bending experiments, relationship between circuit performance and TFT characteristics under mechanical deformation could be elucidated.

• 한국정보과학회논문지:소프트웨어및응용
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• 제34권3호
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• pp.235-245
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• 2007

무인수중로봇은 인간의 직접적인 접근이 제한되는 위험한 지역을 운항하기 때문에 인식, 결정, 그리고 행동과 같은 영역전문가의 고유능력을 수행하는 지능형 제어소프트웨어를 반드시 탑재해야한다. 본 논문에서는 다양한 무인항체에 적용 가능한 RVC 지능시스템 모델을 제안하며, 또한 충돌회피시스템, 항해 계획시스템, 그리고 충돌위험도산출시스템으로 구성된 무인수중로봇을 위한 지능형 자율운항시스템을 개발 한다. 충돌회피시스템에서는 퍼지관계곱에 기반한 장애물회피 알고리즘을 제안하는데 이는 생성경로 관점의 안전성과 효율성을 보장한다. 그리고 항해계획시스템에서는 폴리선을 이용한 항로계획 알고리즘을 제안 한다. 제안된 지능형 자율운항시스템의 성능검증을 위해 환경관리자, 객체, 그리고 3차원뷰어로 구성된 시뮬레이션시스템을 개발하여 시뮬레이션을 수행한다.