• 한국지형학회지
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• 제19권2호
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• pp.173-185
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• 2012

인천 영종도 남부 해안충적평야의 퇴적층을 대상으로 화분분석을 실시하였다. 시료채취 지점(해발고도 7.2m)은 현재 냉온대 중부 낙엽활엽수림대에 속한다. 화분분석에 의해 밝혀진 조사지역 일대의 약 8,900yrBP 이후의 후빙기 환경변화는 다음과 같다. JS-I기(약 8,900~8,500yrB.P.)는 Pinus와 Quercus가 우점하고 일부 Abies와 Picea가 포함된 냉온대 북부의 침활혼효림 시대로서 후빙기 초기의 화분대인 RI대에 대비된다. 이 시대는 현재에 비해 상대적으로 한랭 건조 했다. JS-II기(약 8,500~4,000yrB.P. 어느 시기)는 Pinus가 우점하는 침엽수림시대로서 후빙기 중기 화분대인 RII대에 대비된다. JS-III기(약 4,000yrB.P.경)는 Pinus, Quercus 및 Carpinus가 우점하는 침활혼합림 시대이다. JS-II기와 JS-III기는 JS-I기 보다 상대적으로 온난 습윤했으며, 현재의 기후환경과 유사했을 것으로 생각된다. JS-IV기(약 4,000~900yrBP의 어느 시기)는 Pinus가 우점하는 침엽수림시대로서 후빙기 후기 화분대(RIII) 중에서 RIIIa에 대비된다. JS-V기(약 900yrB.P.~현재)는 2차림인 Pinus가 우점하는 침엽수림시대이다. 이 시대는 수목화분보다 초본화분의 출현율이 높고, 초본류 중에서 Fagopyrum 화분이 연속적으로 출현하는 점 등을 고려할 때, 인간간섭대인 RIIIb에 대비된다. JS-V기부터 조사지역 일대의 거주민들은 본격적인 농경생활을 했던 것으로 생각된다.

• 한국지형학회지
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• 제23권2호
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• pp.1-13
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• 2016

This study analyzed pollen compositions in archaeological site on alluvial plain of Taehwa River, Taehwa-dong, Ulsan-si vegetational and environmental change during 6,200~3,000 yr BP. The results consist upward of Pollen Zone I (Inner Bay environment), Pollen Zone II (Quercus-Alnus stage) and Pollen Zone III (Alnus stage). The pollen grains of Fagopyrum and reddish gray silty horizon including artifacts of the Bronze Age suggest that there was cultivation on dry-field around the study site, although it is not sure that there was paddy-field cultivation.

• 한국지형학회지
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• 제24권3호
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• pp.1-11
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• 2017

The pollen analysis on the deposits of the lower marine terrace I of the estimated paleoshoreline height of 18m was performed in order to estimate the formation age of this terrace developed at the Sanha-dong coast, Ulsan, southeastern coast of the Korean peninsula. The pollen assemblage of the peat layer of SH-1 pollen zone (Quercus-Ulmus/Zelkova zone), lying directly on the marine rounded pebble layer of this terrace, shows that the climatic environment of the deposition period of SH-1 pollen zone was almost similar to that of the Postglacial climatic optimum period, but slightly cooler than that of the late warm stage of Last Interglacial(MIS 5a) in the eastern coast of Korea. This heightens the possibility that the deposition period of the marine rounded pebble layer which was covered by the above SH-1 peat layer is the MIS 5e which has been estimated by a previous study of the sedimentary facies of this terrace deposits (Choi, 2016). The pollen assemblage of SH-2 pollen zone (Pinus-Quercus zone) shows that the climate of this period was almost similar to that of the late Postglacial, but slightly cooler than that of the period of SH-1 pollen zone. This means that the climate around the Sanha-dong was still warmer in the deposition period of the peat layer of SH-2 pollen zone. Thus, the peat layer of SH-2 pollen zone was considered to have been deposited during the period from the early regression stage of the MIS 5d which is the estimated final stage in the deposition period of the above peat layer of SH-1 pollen zone to any stage in which the warmer environment of MIS 5 has still lasted. The humic silt layer of SH-3 pollen zone (Pinus-Ulmus/Zelkova-Abies zone) is assumed to have been deposited during the interstadial of the Last Glacial (MIS 3).

• 한국작물학회지
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• 제65권1호
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• pp.22-29
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• 2020

Rice is very sensitive to high temperatures during the reproductive stage, particularly during the flowering and anthesis periods. To determine how high temperatures result in sterility during the flowering period in Korean rice cultivars, groups of 11 cultivars were subjected to different temperature regimes (24, 28, 30, and 33℃) during the flowering stage using sunlit phytotrons. At an average of 33℃, all 11 rice cultivars reached anthesis earlier than at the other temperatures. Microscopy analyses revealed significant differences in pollen germination and pollen viability in cultivars grown at 33℃ compared to those cultured at lower temperatures. At 33℃, the cultivars had significantly lower fertility rates (47% reduction) than cultivars grown at 24℃. These findings are important as rice pollination and fertility depend on the pollen viability and germination. The present study shows that rice fertility is negatively affected by excessively high temperatures.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1988년도 춘계 학술대회지
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• pp.24-25
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• 1988

• Journal of Plant Biology
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• 제38권1호
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• pp.25-32
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• 1995

The distribution of RNA in the anther wall of Brassica napus during male gametogenesis was followed by 3H-uridine autoradiography. Silver grain(SG) density was not above background in the anther wall just after microspore was released from tetrad callose wall. Significant accumulation of SGs occurred in tapetum, endothecium, and epidermis before microspore vacuolation. Accumulation of RNA in the tapetal cells was peak before the vaculation occurred in the microspore. With the onset of tapetal senescence at the partially vacuolated microspore stage, SGs decreased and they completely disappeared in the tapetum at the bicelled pollen stage. Accumulation of RNA in the endothecial cells was peak after the microspore mitosis and continued just after the generative cell mitosis. Appreciable SGs also occurred in cells of epidermis from nonvacuolate microspore stage to bicelled pollen stage. During this period, SG density was almost same and was not high as compared with tapetum or endothecium. At tricelled mature pollen stage, no incorporation occurred in anther wall. SGs were found mostly over the nucleouls and chromation of the cell nuclei.

• 한국작물학회지
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• 제47권1호
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• pp.62-67
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• 2002

An efficient system of rice microspore culture could contribute to the production of genetically modified rice. The microspores were isolated by mechanical or shed methods. The number of microspores per 100 anthers isolated at uninucleate stage was higher than (or similar to) those at binucleate stage in isolation method with pestle or spatular, but microspore divisions were not easily observed on both stages. On the other hand, pollen division in shed pollen culture was observed more frequently at uninuclear than at binuclear stage. Cold pretreatment at 1$0^{\circ}C$ for 10 days resulted in the best multicellular division to produce microcalli at 12.5% efficiency in shed microspores. Heat shock at 33$^{\circ}C$ for one hour before or after pollen shedding enhanced cell division and callus formation. Out of twelve green regenerants, two were haploids and ten were diploids based on the chromosome analysis of root tips. The size of stoma was 12$^{m}$ m in haploids and 15 ${\mu}{\textrm}{m}$ in diploids determined by scanning electron microscope (SEM).

• The Korean Journal of Ecology
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• 제25권1호
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• pp.45-49
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• 2002

Pollen analyses and $^{14}$ C dating of the sediments of the Akindonuma moor, which is situated in a closed depression of an old landslide, were peformed in order to study the vegetation history of the montane zone of the Miyagi Prefecture, Northeast Japan. The main results are as follows: Two forest zones have been distinguished: 1) the A-I zone, or the Fagus-Quercus-Betula forest (R I stage, before about 11,700 yrs B.P.), and 2) the A-II zone, or the Fagus-Quercus forest (R II stage, after about 11,700 yrs B.P.). By comparing the geological section with the pollen diagram of the moor, it is assumed that the deposit environment of the Middle Part, which mostly consists of peat layers, is very stable. During the period, the Ulmus/Zelfkova pollen ratio was very low and small peak was not recognized. According to the preceding research, theme is a close relationship between the fluctuation of the Ulmus/Zelkova pollen ratio and the general trend of hillslope instability in the changing balance of temporature and precipitation Actually, Zelkova serrata tends to cover the footslope and the lower sideslope. Ulmus davidiana covers the footslope and the alluvial cone. Therefore, the fact that the Ulmus/Zeikova pollen ratio was very low and small peak was not recognized, is believed to reflect the stability of the earth's surface environment, which was estimated from the geological section.

• Journal of Ecology and Environment
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• 제29권6호
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• pp.573-580
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• 2006

한반도 서해안 중부 지역의 식생 변천사 규명을 위해 천리포 수목원 퇴적물의 방사성 탄소 연대 측정과 화분 분석을 실시하였다. 그 결과 약9,300 yr BP 이후 현재까지 식생 변천사를 규명하였다. 분석 결과를 통하여 바닥층부터 표층까지 4개의 주요한 화분대가 설정되었다. : Zone CHL-I, Quercus stage(ca. 9,300$\sim$6,200 yr BP); zone CHL- II, Quercus-Pinus stage(ca. 6,200$\sim$4,600 y BP): zone CHL-III, Pinus-Quercus stage(ca. 4,600$\sim$1,160 yr BP); zone CHL-IV, Pinus stage(ca. 1,160 yr BP-present). 홀로세초기 약9,000$\sim$B,500 yrBP동안 한랭한 기후의 영향으로 냉온대 북부/고산지형 침엽-낙엽혼합림의 단편을 확인할 수 있었다. 약 8,500$\sim$6,000 yr BP 동안 Quercus를 중심으로 한 냉온대 중부/산지형 낙엽활엽수림이 절대적으로 우세한 식생이 유지되고 있었으며, 이러한 식생형의 완성 시기는 약 6,500 y BP 전후로 판단된다. 천리포 수목원 주변에 Pinus가 생육하기 시작한 시기는 약 5,700 yr BP 이후로 사료된다. 약 1,100 yr BP 이후 Pinus의 증가와 비수목화분의 갑작스러운 증가는 인간의 간섭에 의한 식생 ,변화로 보인다. 염습지의 주요한 표징종인 Chenopo-diaceae화분 동태를 통하여 염습지에서 담수호로 변화한 시기는 약 6,500 yr BP이후인 것으로 추정된다.

• Journal of Plant Biotechnology
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• 제4권4호
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• pp.151-154
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• 2002

Conditions for lily pollen growth in vitro and transformation were optimized. Active pollen tube development was achieved effectively in a medium containing 7% sucrose with pH adjusted to 5.7 at the temperature of 27$^{\circ}C$ for about 16-24 hours. Pollen growth was little impaired by the presence of kanamycin at concentration up to 100 mg/L. Pollen rains near the beginning of germination stage were more reliable for Agrobacterium-mediated GUS DNA transformation via vacuum infiltration lasted for 20-40 minutes. GUS DNA integration and its expression in fully developed pollen tubes could be confirmed by Southern blot hybridization, RT-PCR and histochemical staining.