• Neonatal Medicine
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• 제18권2호
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• pp.301-309
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• 2011

목적: Point-of-care test (POCT) 는 신생아 치료에 있어서 중요한 영향을 미칠 잠재력을 가지고 있다. 이 연구의 목적은 신생아 집중 치료실에서 POCT 화학 분석기기의 임상적인 유용성을 살펴보고자 하는데 있다. 방법: 신생아 집중 치료실에 입원한 신생아의 혈액 샘플을 2010년 3월부터 9월까지 POCT 화학 분석기기(Piccolo Xpress Chemistry Analyzer, Abaxis, Union City, CA, USA) 와 중앙 검사실 화학 분석기기(Chemistry analyzer 7600-110, Hitachi Ltd., Tokyo, Japan)를 이용하여 분석하였다. POCT와 중앙 검사실 기계 간의 15가지 항목에 대하여 상관 관계를 평가하였다. 또 POCT의 일치도를 알아보기 위해 세 번의 연속적인 혈액 검사를 시행하고, 세 검사간의 차이를 기록하여 평가하였다. 수행 오류가 있는 경우에는 log file을 통해서 오류의 원인을 파악하였다. 결과: 54명의 신생아에서 정확도를 위해 시행된 112쌍의 연구 중 오류가 없던 100쌍의 결과에서 두 기계 간의 높은 상관 관계를 보였다. 112쌍의 연구 도중 12개의 오류가 발생하였다. 가장 흔한 원인은 불충분한 샘플 오류였다. 일치도 검증을 위한 18명의 환아들에서 시행한 18개의 삼중 분석에서는 3-10%의 오차율이 나타났고, 이는 수용할 만한 것이었다. 54개의 분석 중 오류는 일어나지 않았다. 결론: 이 POCT는 짧은 시간 내에 소량의 전혈을 이용하여 다양한 항목을 검사할 수 있다. 비록 오류가 나타날 수 있지만, 이는 예방 가능한 것으로 사료된다. 이 POCT는 덜 침습적인 방법으로 소량의 혈액을 채취하기 때문에 신생아 집중 치료실에서 간단하고 빠른 진단에 유용하다.

• 한국임상수의학회지
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• 제37권1호
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• pp.23-27
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• 2020

Between May and November 2018, babesiosis was examined in 162 bloods samples obtained to an animal hospital in Jeju island for anemia and medical examination. Sixty-two of 162 (38.3%) were positive by PCR. The ultra fast real-time PCR test with blood directly analyzed without DNA extraction showed the same results. Accurate diagnosis, treatment and prognosis of babesiosis should be combined with clinical symptoms, blood tests, the babesia antibody test, and the PCR antigen test. Ultra fast real-time PCR, with these tests, is expected to be a point-of-care testing (POCT) for easy, fast and accurate diagnosis of babesiosis in the veterinary clinic.

• 대한디지털의료영상학회논문지
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• 제10권2호
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• pp.33-37
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• 2008

To keep the online medical records available to anyone without constraint of time and space, introducing EMR (Electronic medical record), which is a clinical support management system. The purpose of this study is to develop interface standard of clinical test device. Integration and sharing of medical information is faced with enormous obstacles because medical organizations and associated companies are separately developing the interface. I hope that multi-function management system with workstation concept is operated to efficiently transmit clinical device result data based on this study. Transfer of precise medical result data available for decision making will improve quality of health care service.

• 대한의용생체공학회:의공학회지
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• 제42권3호
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• pp.125-142
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• 2021

The POCT (point-of-care test) sensing that has been a fast-developing field is expected to be a next generation technology in health care. The POCT sensors for the detection of proteins, small molecules and especially nucleic acids have lately attracted considerable attention. According to the World Health Organization (WHO), the POCT methods are required to follow the ASSURED guidelines (Affordable, Sensitive, Specific, User- friendly, Robust and rapid, Equipment-free, Deliverable to all people who need the test). Recently, several CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) based diagnostic techniques using the sensitive gene recognition function of CRISPR have been reported. CRISPR/Cas (Cas, CRISPR associated protein) systems based detection technology is the most innovative gene analysis technology that is following the ASSURED guidelines. It is being re-emerged as a powerful diagnostic tool that can detect nucleic acids due to its characteristics that enable rapid, sensitive and specific analyses of nucleic acid. The first CRISPR-based diagnosis begins with the discovery of the additional function of Cas13a. The enzymatic cleavage occurs when the conjugate of Cas protein and CRISPR RNA (crRNA) detect a specific complementary sequence of the target sequence. Enzymatic cleavage occurs on not only the target sequence, but also all surrounding non-target single-stranded RNAs. This discovery was immediately utilized as a biosensor, and numerous sensor studies using CRISPR have been reported since then. In this review, the concept of CRISPR, the characteristics of the Cas protein required for CRISPR diagnosis, the current research trends of CRISPR diagnostic technology, and some aspects to be improved in the future are covered.

• 농촌의학ㆍ지역보건
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• 제47권4호
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• pp.220-228
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• 2022

119 구급대원들이 사용하는 현장 검사 장비 중 하나인 자가 혈당측정기는 결과 값이 정확해야 환자에게 적절한 처치를 제공할 수 있다. 연구 최초 조사 시점 이후 현재까지도 119 구급대원 현장업무 지침에 현장 검사 장비의 작동 유무, 수량 등을 확인하는 것은 있으나, 장비의 점검에 수량, 작동 여부 등은 있으나 본 연구에서 분석한 정도관리 내용은 없다. 현장검사 장비의 질 관리가 되지 않고 있다는 것은 환자에 대한 부정확한 평가와 이로 인한 불필요한 처치와 과소, 과대처치 또는 환자 상태가 악화될 가능성을 높일 수 있다. 본 연구를 통해, 앞으로 119 구급대원의 혈당측정기를 포함한 모든 현장검사 장비의 사용실태조사가 이루어져야 하고, 119 구급대 현장 업무 지침에 현장 검사 장비 정도관리를 추가하여야 하겠다. 또한, 현장 검사 장비가 관련 지침에 따라서 적절한 질 관리가 이루어져야 하고, 지역에서 관련 업무 전문가나 응급의료지도를 담당하고 있는 지도의사에게 관리되는 것이 합당하겠다.

• 대한임상독성학회지
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• 제9권1호
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• pp.26-29
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• 2011

Purpose: The role of a point of care test (POCT) is currently becoming important when treating patients and making decisions in the emergency department. It also plays a role for managing patients presenting with drug intoxication. But the availability of the test has not yet been studied in Korea. Therefore, we investigated the utility and the availability of POCT for drug screening used in the emergency department. Methods: This was a retrospective study for those patients with drug intoxication between January 2007 and December 2010 in an urban emergency department. Results: Between the study period, 543 patients were examined with a Triage$^{(R)}$-TOX Drug Screen. Among those, 248 (45.7%) patients showed negative results and 295 (54.3%) patients showed positive results. The sensitivity of the test for benzodiazepine, acetaminophen and tricyclic antidepressants were 85.9%, 100%, 79.2%, respectively. Conclusion: POCT of drug screening in emergency department showed good accuracy especially in patient with benzodiazepine, acetaminophen and tricyclic antidepressant intoxication. Therefore, it can be useful diagnostic tool for the management of intoxicated patients.

• 세라미스트
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• 제21권3호
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• pp.293-301
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• 2018

Paper-based analytical devices (${\mu}PAD$) are highly advantageous for portable diagnostic systems owing to their low costs and ease of use. ${\mu}PADs$ are considered as the best candidates for realizing the World Health Organization (WHO) ASSURED criteria: affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free, and deliverable to end users. However, they have several limitations such as low sensitivity and accuracy. This article reports a mini review for a micro-fluidic paper-based analytical devices (${\mu}PAD$), especially for addressing low sensitivity and accuracy issues.

• 한국콘텐츠학회논문지
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• 제14권3호
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• pp.52-58
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• 2014

현장검사는 환자가 있는 즉석에서 검사하여 결과를 알 수 있으며 신속하고 경제적으로 시행할 수 있는 장점이 있어 널리 이용되고 있다. 특히 면역크로마토그래피법을 이용한 검사 항목은 편리하게 쓰이고 있으나 오류가 발생하여 재확인 할 경우, 판독선이 시간이 경과됨에 따라 재확인이 불가능한 단점이 있고 반응이 약한 경우 판독자에 따라 오류가 생길 수 있다. 이에 검사에 쓰이는 다양한 현장검사용 스트립의 판독이 가능하고 화상을 저장할 수 있는 기능을 가진 멀티 스트립 리더기를 개발하였고, 요 임신검사가 의뢰된 검체를 대상으로 개발된 멀티 스트립 리더기를 평가한 결과 100% 일치된 결과를 보였다. 본 연구에서 개발된 멀티 스트립 리더기는 실제 임상에서 편리하고 경제적으로 사용될 수 있을 것으로 생각된다.

• Journal of Microbiology and Biotechnology
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• 제23권1호
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• pp.1-6
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• 2013

Tetanus is a specific infectious disease, which is often associated with catastrophic events such as earthquakes, traumas, and war wounds. The obligate anaerobe Clostridium tetani is the pathogen that causes tetanus. Once the infection of tetanus progresses to an advanced stage within the wounds of limbs, the rates of amputation and mortality increase manifold. Therefore, it is necessary to devise a rapid and sensitive point-of-care detection method for C. tetani so as to ensure an early diagnosis and clinical treatment of tetanus. In this study, we developed a detection method for C. tetani using loop-mediated isothermal amplification (LAMP) assay, wherein the C. tetani tetanus toxin gene was used as the target gene. The method was highly specific and sensitive, with a detection limit of 10 colony forming units (CFU)/ml, and allowed quantitative analysis. While detecting C. tetani in clinical samples, it was found that the LAMP results completely agreed with those of the traditional API 20A anaerobic bacteria identification test. As compared with the traditional API test and PCR assay, LAMP detection of C. tetani is simple and rapid, and the results can be identified through naked-eye observation. Therefore, it is an ideal and rapid point-of-care testing method for tetanus.

• Allergy, Asthma & Respiratory Disease
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• 제6권6호
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• pp.310-314
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• 2018

Purpose: Conventional serum IgE assay was costly, required the skills of expert, and relied heavily on expensive equipment. Quantitative measurement of total IgE using Point of Care Test (POCT) device can be the solution for these limitations. This study evaluated and validated the reproducibility of ImmuneCheck IgE. Methods: This study included 120 patients of allergic diseases such as allergic rhinitis, asthma, drug allergy, food allergy, atopic dermatitis, or anaphylaxis. The reliability of POCT ImmuneCheck IgE was evaluated by comparing results from the naked eye and from the Q-Reader. Intratest reproducibility and intertest correlation were analyzed using intraclass correlation coefficient (ICC). Results: Of the 120 enrolled patients, 51 were males and 69 were females. The ages ranged from 19 to 84 years, with an average age of 51.5 years. The concentration of serum total IgE measured by Phadia ImmunoCAP IgE ranged from 5.95 to 5,000 IU/mL. ICC for Intratest reproducibility of ImmuneCheck IgE by naked eye and by Q-Reader were 0.991 (P< 0.001) and 0.989 (P< 0.001), respectively. In addition, intertest correlation between ImmuneCheck IgE and Phadia ImmunoCAP IgE results of naked eye and Q-Reader were 0.968 (P< 0.001) and 0.948 (P< 0.001), respectively. Conclusion: The ImmuneCheck IgE was reproducible and highly correlated with conventional Phadia ImmunoCAP IgE assay. This result suggests that ImmuneCheck IgE can be a useful tool for rapid and precise detection of total IgE.