• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.2
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• pp.507-516
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• 2004

Alzheimer's disease(AD) is a geriatric dementia that is widespread in old age. In the near future AD will be the commom disease in public health service. Although a variety of oriental presciptions in study POD(Polygala tenuifolia extracted from dichlorometan) have been traditionally utilized for the treatment of AD, their pharmacological effects and action mechanisms have not yet fully elucidated. It has been widely believed that AP peptide divided from APP causes apoptotic neurotoxicity in AD brain. However, recent evidence suggests that CT105, carboxy terminal 105 aminoacids peptide fragment of APP, may be an important factor causing neurotoxicity in AD. SK-N-SH cells expressed with CT105 exhibited remarkable apoptotic cell damage. Based on morphological observations by phase contrast microscope and NO formation in the culture media, the CT105-induced cell death was significantly inhibited by POD. In addition, AD is one of brain degeneration disease. So We studied on herbal medicine that have a relation of brain degeneration. From old times, In Oriental Medicine, PO water extract has been used for disease in relation to brain degeneration. We were examined by ROS formation, neurite outgrowth assay and DPPH scravage assay. Additionally, we investigated the association between the CT105 and neurite degeneration caused by CT105-induced apoptotic response in neurone cells. We studied on the regeneratory and inhibitory effects of anti-Alzheimer disease in pCT105-induced neuroblastoma cell lines by POD. Findings from our experiments have shown that POD inhibits the synthesis or activities of CT105, which has neurotoxityies and apoptotic activities in cell line. In addition, treatment of POD(>50 ㎍/㎖ for 12 hours) partially prevented CT(105)-induced cytotoxicity in SK-N-SH cell lines, and were inhibited by the treatment with its. POD(>50 ㎍/㎖ for 12 hours) repaired CT105-induced neurite outgrowth when SK-N-SH cell lines was transfected with CT105. As the result of this study, In POD group, the apoptosis in the nervous system is inhibited, the repair against the degerneration of Neuroblastoma cells by CT105 expression is promoted. Decrease of memory induced by injection of scopolamin into rat was also attenuted by POD, based on passive avoidance test. Taken together, POD exhibited inhibition of CT105-induced apoptotic cell death. POD was found to reduce the activity of AchE and induced about the CA1 in rat hippocampus. Base on these findings, POD may be beneficial for the treatment of AD.

• Korean Journal of Environmental Agriculture
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• v.19 no.5
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• pp.382-393
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• 2000

The principal indicators contributing to water qualities was screened by factor analyses, based on the monitored chemical parameters of water quality for various water resources from 1995 to 1999 in the small agricultural catchments of the Han River Basin. Water samples of streams, groundwaters, and reservoirs were taken four times a year from upper (Daegwanryong), middle (Dunnae and Chunchon) and lower (Guri) reaches of Han River Basin. In these areas, the respective type of farming practiced was alpine agriculture and livestocks raising, typical upland and paddy cultivation, and intensive cropping in the plastic film house. Water quality was monitored for twenty-one water quality parameters, including pH, EC, SS, T-N, T-P, COD, cations, anions, and heavy metals. pH, EC and COD of the stream waters were suitable for the Korea irrigation water quality guidelines. However, T-N and T-P concentrations of water samples in four catchments far exceeded the irrigation water guideline. Concentrations of canons and heavy metals in Wangsuk stream in Guri area were higher than those in streams in other areas. Factor analysis revealed that significant correlation was observed for 81 pairs out of 231 water quality indicators of stream water among the $21\;{\times}\;21$ cross correlation matrix of stream water quality indicators. The first factor accounted for 27.01% of the total variation in stream water quality indicators, and high positive factor loadings were shown on EC, K, Na, $NH_4\;^+-N$, $PO_4\;^{3-}$, $SO_4\;^{2-}$, and COD. Fifty-three water quality indicator pairs were significant out of 190 ground water quality parameters. The first factor accounted for 28.54% of the total variation in ground water quality indicators, and high loadings were revealed on EC, Ca, Mg, K, Na, $NH_4\;^+-N$, and $SO_4$. Twenty-nine pairs of reservoir water quality indicators were significant out of 66 pairs. The first factor accounted for 37.06% of the total variation in reservoir water quality indicators, and high loadings were shown on EC, Mg, K, Na, SS, T-P, Cl, and COD. These results demonstrate that EC was the first factor contributing to water quality.

• Korean Journal of Environmental Agriculture
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• v.11 no.1
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• pp.41-49
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• 1992

To determine the efficacy of uniconzaole[(E)-1-(4-chlorophenyl)-4,4-dimethy 2-(1,2,4-triazol-1-yl)-1-penten-3-ol)](XE-1019) as a phytoprotectant against $O_3$ injury in tomato plants(Lycopersicon esculentum Mill. 'Pink Glory'), plants were given a 50ml soil drench of uniconazole solution at concentrations of 0.001, 0,01, 0.1 and 0.2mg/pot thirteen days prior to $O_3$ fumigation. All four uniconazole concentrations were effective in providing protection against $O_3$ exposure(16h at 0.3 ppm). Uniconazole treatment above 0.001 mg/pot significantly reduced stem elongation, leaf enlargement, leaf area and fresh weight of plant, whereas increased chlorophyll concentration. Transpiration rate on a whole plant basis was reduced by uniconazole treatment and $O_3$ exposure. Uniconazole reduced ethylene production induced by $O_3$ injury but had little or no effect on defoliation of cotyledons and leaf epinasty. Activities of peroxidase (POD) and superoxide dismutase(SOD) were slightly increased by application of uniconazole. With increasing exposure time, $O_3$ increased POD activity but decreased SOD activity. The phytoprotective effects of uniconazole were diminished by applying gibberellin at $10{\sim}20$ ppm. These results suggest that the phytoprotective effects of uniconazole are related to its role of increasing activities of free radical scavengers such as POD and SOD, in addition to growth-retardation as an anti-gibberellin.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.8
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• pp.105-114
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• 2018

Membrane fouling in EC-MBR (Electro-Coagulation aided Membrane Bio-Reactor) processes was evaluated according to the operating parameters, such as current density and contact time. In addition, the fouling mechanism was investigated. Compared to the control (i.e., no electro-coagulation), membrane fouling for filtration of the activated sludge suspension after electro-coagulation was reduced significantly. Membrane fouling was improved further when the contact time was doubled under a low current density of $2.5A/m^2$. On the other hand, membrane fouling was not mitigated further, as expected, even though the contact time was doubled from 12 to 24 hr. at a current density of $10A/m^2$. This indicates that the overall decrease in membrane fouling is a function of the product of the current density and contact time. The particle size of the activated sludge flocs after electro-coagulation was changed slightly, which means that the membrane fouling reduction was not attributed to a larger particle size resulting from electro-coagulation. The experimental confirmed that the dynamic membrane made from aluminum hydroxide, Al(OH)3, and/or aluminum phosphate, Al(PO4), which had been formed during the electro-coagulation, played a key role on the reduction of membrane fouling. The dynamic membrane prevents the particles in the feed solution from deposition to the membrane pores and cake layers. Dynamic membrane formation as a result of electro-coagulation plays a critical role in the mitigation of membrane fouling in EC-MBR.

• The Korean Journal of Physiology
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• v.24 no.1
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• pp.51-65
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• 1990

The purpose of the present study was to examine the hemodynamic responses, especially in arterial and skin blood flows, in conjunction with the changes of plasma catecholamine levels as an indirect marker of adrenergic tone during the early stage of head-down tilt (HDT), and to evaluate the early physiological regulatory mechanism in simulated weightlessness. Ten mongrel dogs, weighing8\;{\sim}\;14\;kg, were intravenously anesthetized with nembutal, and postural changes were performed by using the tilting table. The postural changes were performed in the following order: supine, prone, HDT $(-6^{\circ}C)$ and lastly recovery prone position. The duration of each position was 30 minutes. The measurements were made before, during and after each postural change. The arterial blood flow $({\.{Q}})$ at the left common carotid and right brachial arteries was measured by the electromagnetic flowmeter. Blood pressure (BP) was directly measured by pressure transducer in the left brachial artery. To evaluate the peripheral blood flow, skin blood flow $({\.{Q}})$ was calculated by the percent changes of photoelectric pulse amplitude on the forepaw, and skin temperature was recorded. The peripheral vascular resistance (PR) was calculated by dividing respective mean BP values by ${\.{Q}}$ of both sides of common carotid and brachial arteries. Heart rate (HR), respiratory rate (f) and PH, $Po_{2},\;Pco_{2}$ and hematocrit of arterial and venous blood were also measured. The concentration of plasma epinephrine and norepinephrine was measured by radioenzymatic method. The results are summarized as follows: Tilting to head-down position from prone position, HR was initially increased (p<0.05) and BP was not significantly changed. While ${\.{Q}}$ of the common carotid artery was decreased (p<0.05) and PR through the head was increased, ${\.{Q}}$ of the brachial artery was increased (p<0.05) and PR through forelimbs was decreased. ${\.{Q}}$ of the forepaw was initially increased (p<0.05) and then slightly decreased, on the whole revealing an increasing trend. Plasma norepinephrine was slightly decreased and the epinephrine was slightly increased. f was increased and arterial pH was increased (p<0.05). In conclusion, the central blood pooling during HDT shows an increased HR via Bainbridge reflex and an increased ${\.{Q}}$ of the forepaw and brachial ${\.{Q}}$, due to decreased PR which may be originated from the depressor reflex of cardiopulmonary baroreceptors. It is suggested that the blood flow to the brain was adequately regulated throughout HDT $(-6^{\circ}C)$ in spite of central blood pooling. And it is apparent that the changes of plasma norepinephrine level are inversely proportional to those of ${\.{Q}}$ of the forepaw, and the changes of epinephrine level are paralleled with those of the brachial ${\.{Q}}$.

• Ocean and Polar Research
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• v.27 no.1
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• pp.15-24
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• 2005

In order to investigate the structures and dynamics of phytoplankton communities, each physicochemical environmental factor, species composition, standing crop, and dominant species were examined in the marine ranching ground of Tongyeong coastal waters from April to October, 2000. During the studies, mean water temperature and salinity were $18.8^{\circ}C$ and 33.1 psu, respectively. DO, SS and transparency varied from 5.43 to 11.39 mg/l, 14.6 to 32.4mg/l and 3.5 to 9.0m, respectively. Light intensities varied from 0.02 to $966{\mu}E/m^2/s$, which decreased with depth. $NH_4-N,\;NO_3-N,\;NO_2-N,\;PO_4-P,\;and\;SiO_2-Si$ were fluctuated from 0.059 to 0.332 mg/l, 0.040 to 0.800 mg/l, 0.001 to 0.468 mg/l, 2.3 to $143.0{\mu}g/l$, and 0.007 to 0.600 mg/l, respectively. chlorophyll a concentrations were fluctuated from 0.7 to $8.9{\mu}g/l$. Among 130 taxa of phytoplankton communities observed. diatoms occupied more than 81.54% of the total species, and the others were dinoflagellates and silicoflagellates. Phytoplankton standing crops ranged from $4.6{\times}10^4\;to\;2.6{\times}10^6cells/l$. In October, the standing crops were at bloom level showing more than $10^6cells/lat$ all stations. Dominant species changed by month and station. Leptocylindrus danicus occupied 59.84% in April and 22.03% in June. Pseudo-nitzschia pungens in August and Chaetoceros socialis and Skeletonema costatum in October were predominant species. In order to investigate factors influencing the total phytoplankton standing crops the correlations between the standing crops of diatoms, dinoflagellates, all phytoplanktons occurred and environmental factors were calculated using a multiple regression analysis. The coefficient of determination $(R^2)$ for total standing crops was 0.63 which explained 63% of variance and that of $R^2$ for diatom was 0.82. In statistical analysis, the results showed that the environmental factors influencing the size of the communities were predominantly water temperature, salinity and silicate.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.23 no.3
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• pp.163-171
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• 2017

Effects of different packaging methods for maintaining the shelf life and postharvest quality of iceberg lettuce (Lactuca sativa L.) were studied after harvesting in summer season. Lettuce heads were packaged in plastic crate with or without different films such as (A) Individual lettuce head sealed packaging with linear low density polyethylene (LLDPE) film; (B) Packaging lettuce head in plastic crate and wrapped with LLDPE film; (C): Individual lettuce head sealed packaging with perforated high density polyethylene (HDPE) film; (D) Packaging lettuce head in plastic crate and wrapped with perforated HDPE; and (E) Packaging lettuce head in plastic crate without any film (control), and stored at $2^{\circ}C$ for 35 days. Several quality parameters such as fresh weight loss, SPAD (soil & plant analyzer development) meter value, respiration rate, moisture content and appearance of lettuce were investigated. The lettuce wrapped with individually-sealed LLDPE film showed the lowest weight loss and the highest SPAD value rendering the best appearance index among the treataments throughout the three-week storage period at $2^{\circ}C$. Extending the freshness of iceberg lettuce during low temperature storage will definitely increase the salability potential in the domestic market even during summer season.

• Bulletin of the Korean Chemical Society
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• v.35 no.8
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• pp.2342-2348
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• 2014

Poly(lactic-co-glycolic acid) (PLGA) were grafted to both ends of Pluronic$^{(R)}$ F68 ($(EO)_{75}(PO)_{30}(EO)_{75}$) triblock copolymer to produce poly{(lactic acid)$_m$-co-(glycolic acid)$_n$}-b-poly(ethylene oxide)$_{75}$-b-poly(propylene oxide)$_{30}$-b-poly(ethylene oxide)$_{75}$-b-poly{(lactic acid)$_m$-co-(glycolic acid)$_n$} (PLGA-F68-PLGA) pentablock copolymers. Molecular weights of PLGA blocks were controlled and five kinds of pentablock copolymers with different PLGA block lengths were synthesized using in-situ ring-opening polymerization of D,L-lactide and glycolide with tin(II) 2-ethylhexanoate ($Sn(Oct)_2$) catalyst. PLGA-F68-PLGA pentablock copolymers were characterized by $^1H$- and $^{13}C$-NMR, GPC, and TGA. The numbers (2m, 2n) of repeating units for lactic acid and glycolic acid inside PLGA segments were obtained as (48, 17), (90, 23), (125, 40), (180, 59), and (246, 64), with $^1H$-NMR measurement. From NMR data, the resultant molecular weights were determined in the range of 12,700-29,700, which were similar to those obtained from GPC. Polydispersity index was increased in the range of 1.32-1.91 as the content of PLGA blocks increased. TG and DTG thermograms showed discrete degradation traces for PLGA and F68 blocks, which indicate the weight fractions of PLGA blocks in pentablock copolymers can be calculated by TG profile and it is possible to remove PLGA block selectively. Hydrodynamic radius and radius of gyration of pentablock copolymer micelle were obtained in the range of 46-68 nm and 31-49 nm, respectively, in very dilute (i.e. 0.005 wt %) aqueous solution of THF:$H_2O$ = 10:90 by volume at $25^{\circ}C$.

• KSBB Journal
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• v.15 no.2
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• pp.174-180
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• 2000

Pichia pastoris and Hansenula polymorpha, the methylotrophic yeasts have been widely used as a host for the production of e eudaryotic proteins due to the advantages related to their inherited characters. This paper describes the method to enhance t the productivity of recombinant proteins by P. pastoris and H. po$\psi$morpha. In the production of recombinant proteins using a f fed batch fermentation system, the effects of specific growth rate on the specific expression rate of re$\infty$mbinant proteins w were studied. In both species, the expression system of recombinant proteins using the fed batch fermentation was optimezed.

• The Korean Journal of Pharmacology
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• v.32 no.1
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• pp.75-82
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• 1996

The hypothesis that calcium provoke $O_2^-$ formation by Kupffer cells and may contribute to carbon tetrachloride $(CCl_4)$ induced liver injury was studied in SD rats. In $CCl_4-treated$ animals, hepatic malonaldehyde (nmole/gm liver) and plasma ALT (IU/ml) levels elevated significantly from $119.63{\pm}13.00$ to $268.97{\pm}14.82$ and from $17.3{\pm}0.18$ to $806.08{\pm}37.63$, respectively, compared to those in controls. Activation of Kupffer cells with high dose of retinol (250,000 IU/kg/day, po, for 7 day) significantly enhanced ALT levels, while inactivation of Kupffer cells with gadolinium chloride (7.5 mg/kg/day, ip, for 2 day) attenuated the increase of serum ALT level following $CCl_4$ treatment. Diltiazem (10 mg/kg/day, ip for 2 day) given in combination with retinol led to a marked decrease in ALT levels compare to the level in rats treated only with retinol against $CCl_4$ treatment. In order to determine any alterations in cytochrome P450 activities, the P450 content and the CYP2E1 activity were measured and all $CCl_4-treated$ rats showed significantly lower levels compared to those in controls and vehicle-treated animals. There were significant increases in glutathione peroxidase in all $CCl_4-treated$ rats except diltiazem treated groups. No difference was found among untreated and vehicle-treated rats. It is concluded that Kupffer cells contribute to $CCl_4-induced$ liver injury and that calcium antagonist attenuated the increased $CCl_4-induced$ liver injury due to activation of Kupffer cells.