• Title/Summary/Keyword: Plutella xylostella L.

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Antifeeding Activity and Chitin Synthesis Inhibition Activity of the Ultrasonic Treatment on Plant Extract against Plutella xylostella(Lepldoptera : Yponomeutidae) (초음파 처리 식물체의 배추좀나방 유충에 대한 섭식 저해활성 및 카틴합성 저해활성)

  • 방혜선;이완주;황석조;구태원;김선여
    • Journal of Life Science
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    • v.9 no.3
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    • pp.248-252
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    • 1999
  • Effects of the ultrasonic treatment on plant represents antifeeding activity and chitin synthesis inhibition activity against diamondback moth (Plutella xylostella L.) by a leaf-dipping method at a concentration of 5,000ppm. Methanol extracts of leaves of the ultrasonic treatment on plant was sequentially partitioned with n-hexane, chloroform, ethlyacetate, butanol and water fractions, and bioassayed. Antifeeding activities were observed in n-hexane, chloroform and ethlyacetate fractions. N-hexane fraction has 94.6% of strongest antifeeding activity against P. xylostella. From the n-hexane sequentially partitioned showed H6 and H8 fractions of antifeeding activities against P. xylostella. And chitin synthesis inhibition activity against P. xylostella was obtained the methaol, n-hexane and water fractions.

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Diamondback moth (Plutella xylostella L.) resistance to organophosphorus and carbamate insecticides in Kangwon alpine vegetable croplands (강원도 고랭지대 배추경작지 배추좀나방(Plutella xylostella L.)의 유기인계 및 카바메이트계 살충제에 대한 저항성 발달)

  • Cho, Jun-Mo;Kim, Kyoung-Ju;Kim, Song-Mun;Han, Dae-Sung;Hur, Jang-Hyun
    • The Korean Journal of Pesticide Science
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    • v.5 no.1
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    • pp.30-35
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    • 2001
  • Diamondback moth (Plutella xylostella L.) recently became a single worst insect which is not controlled effectively by organophosphorus and carbamate insecticides in Kangwon alpine croplands. The objective of this study was to determine if diamondback moth has developed a resistance to organophosphorus and carbamate insecticides. Resistance of diamondback moth, collected at Keichon, Jangpyong Taebaek, Chunchon, and Hongchon, was determined by the concentration required to kill fifty percent of population, $LC_{50}$. Their response of resistance varied to insecticides and locations: Taebaek populations were 35 and 70 times more resistant to chlorpyrifos and fenitrothion, respectively, than susceptible(S) population. Hongchon populations were 94 and 254 times more resistant to chlorpyrifos and fenitrothion, respectively, than S population. In addition, Chunchon populations were 37 and 19 times more resistant to profenofos and benfuracarb, respectively, than S population. However, the field populations did not differ in resistance to diazinon, phenthoate, flupyrazofos, carbofuran, and furathiocarb. This study show that field populations of diamondback moth found in Kangwon alpine vegetable croplands have developed a resistance and/or multiple resistance to some insecticides, implying that farmers are losing organophosphorus and carbamate insecticide options for selective control in vegetable crops.

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Structural Characteristics that Influence on the Insecticidal Activity of 2-(n-Octyl)pseudothiourea Analogues against the Diamondback Moth (Plutella xylostella, L.)

  • Soung, Min-Gyu;Kil, Mun-Jae;Sung, Nack-Do
    • Bulletin of the Korean Chemical Society
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    • v.30 no.11
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    • pp.2749-2753
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    • 2009
  • Structural characteristics that influence on the insecticidal activity ($pI_{50}$) of 2-(n-octyl)isothiourea analogues (1-45) against the diamondback moth (Plutella xylostella, L.) based on three dimensional quantitative structure activity relationships (3D-QSARs) were discussed quantitatively using a comparative molecular field analysis (CoMFA) and a comparative molecular similarity indeces analysis (CoMSIA) methods. The statistical values of the CoMFA 2 model were better than those of the CoMSIA 1 model. The CoMFA 2 model was the optimized model with the correlativity (the training set: Ave. = 0.104 & PRESS = 0.613) and the predictability (the test set: Ave. = 0.086 & PRESS = 0.096). Insecticidal activities with the optimized CoMFA 2 model were dependent upon steric factors (79.4%) of $R_1-R_3$ substituents. From the analytical results of CoMFA contour maps, it is predicted that the R1 substituent of 1-45 which has a steric favor in a broad space, $R_2\;and\;R_3$ groups with a steric favor in a narrow space and a H-bond donor favor would have better the insecticidal activity.

Evaluation of Insecticidal Activity of Plant Extracts against the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae) on Vegetable Plant (배추좀나방(Plutella xylostella)에 대한 식물추출물의 살충력 평가)

  • Choi, I-Jin;Kwon, Hyuk-Hyun;Lee, Han-Ho;Son, Hyoung-Gi;Hong, Sang-Kil;Kang, Jong-Woon;Park, Yu-Soon
    • Korean Journal of Plant Resources
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    • v.26 no.1
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    • pp.19-25
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    • 2013
  • This study focused on the usefulness of plant extracts for a city organic farm. Insecticidal activity of 8 plant species, plants crude extracts by ethanol and water, against third instar larvae (susceptible strain) of Plutella xylostella was evaluated in this study. Ethanol(25%) extracts of plant materials had better insecticidal activity than water extracts at the room temperature. Insecticidal activity were investigated by leaf disc spray method. Five plant extracts (Capsicum annuum, Chelidoniun majus, Leonurus sibiricus, Coptis japonica, Mentha piperascens) showed over 50% insecticidal activity to P. xylostella at the concentration of $1500mg{\cdot}L^{-1}$. Extract of C. annuum showed the highest insecticidal activity (83.3%) against P. xylostella. Phytotoxicity was not observed on Raphanus sativus and Brassica campestris with spray application. Seed germination and growth of two plants were not affected. Extract of C. japonica revealed 73.3% control efficacy against P. xylostella of R. sativus and showed 70% control efficacy against those of B. campestris, respectively, in plastic house. Insecticidal activity against various insects was observed in C. japonica extract as 87.4% for R. sativus and 74.3% for B. campestris in the field. These results suggested that extracts of C. annuum, C. japonica showed the highest insecticidal activity, and could be used as city farm insecticides for organic farming.

Characterization of Bacillus thuringiensis StrainBT-14 having Insecticidal Activity against Plutella xylostella

  • Bok, Song-Hae-Jung, Yong-Chul;Kim, Sung-Uk;Son, Kwang-Hee;Lee, Hyung-Hoan
    • Journal of Microbiology and Biotechnology
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    • v.4 no.4
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    • pp.322-326
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    • 1994
  • Bacillus thuringiensis strain BT-14 was isolated from alfalfa dust in Korea. The strain BT-14 produced one bipyramidal crystal and one spore in the cell. The biochemical characteristics of the strain BT-14 were similar to those of Bacillus thuringiensis subsp. kurstaki HD-l. Examination of its antibiotic resistance revealed that while the strain BT-14 was less resistant than BTK HD-l to ampicillin, gentamycin, neomycin and tobramycin, it was more resistant to amikacin than BTK HD-l. The $\delta$-endotoxin crystal of strain BT-14 consisted of a single protein with a high molecular weight of ca 135 KD on a 10% SDS-PACE. The strain BT-14 contained at least nine different plasmids with sizes of 2.9, 5.3, 5.8, 6.2, 9.4, 15.1, 18.1, 23.1 and 79 Kb. In insect bioassay, the isolated strain BT-14 showed lethality of 67% against Plutella xylostella larvae at dilution of 5$\times$$l0^{-4}$ (5$\times$l0 to 3$\times$$l0^2$ spores/ml), which is, almost equivalent to that of BTK HD-l.

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Characteristics of resistance to chlorpyrifos in diamondback moth(Plutella xylostella L.) collected from Chinese cabbage alpine farmland at Gangwon-do, Korea (강원도 고랭지 Chlorpyrifos 포장저항성 배추좀나방(Plutella xylostella L.)의 저항성 특성)

  • Cho, Jun-Mo;Kim, Kyung-Joo;Kim, Seong-Soo;Park, Hong-Ryeol;Lim, Chun-Keun;Hur, Jang-Hyun
    • The Korean Journal of Pesticide Science
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    • v.10 no.1
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    • pp.50-55
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    • 2006
  • A field-resistant strain of the diamondback-moth(Plutella xylostella L.), collected from Chinese cabbage alpine farmland at Gangwon-do, Korea, was used for determination of the characteristics of resistance to chlorpyrifos using the activities of esterases and glutathione-S-transferase(GST), protein sequestration and AChE insenstivity. Although the activities of esterases extracted from resistant strain and susceptible strain were not significantly different, isozyme bands shown on the electrophoresis were different. GST activity from field resistant strain was 1.5-fold higher than that of susceptible. No differences were shown between resistant and susceptible ones in protein sequestration. The insensitivities of AChE to chlorpyrifos, however, extracted from susceptible strain was 460-fold higher than those of resistant. These results indicated that the insensitivity of AChE is the major factor for developing the resistance and activities of GST might be a minor factor.

Development of a Bioassay Method Using Aluminium Foil Sheet for Screening Ovicidal Activity Against Diamondback Moth Egg, Plutella xylostella L. and Selection of Plant Extracts with High Ovicidal Activity (알루미늄호일 이용 배추좀나방(Plutella xylostella L.) 살란활성 검정법 개발 및 살란활성 식물추출물의 선발)

  • Kwon, Min;Kim, Ju-Il;Lee, Seung-Hwan;Lin, Mei-Ying
    • The Korean Journal of Pesticide Science
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    • v.15 no.2
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    • pp.194-200
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    • 2011
  • Diamondback moth (DBM, Plutella xylostella L.) is known as the most destructive pest of cruciferous crops worldwide. As most insecticides targeted to mainly larval stage, new insecticides which have hatching-inhibitory or ovicidal activity could be more efficient to control DBM. Therefore, we developed an easy and efficient method for screening ovicidal activity of DBM eggs using aluminum foil. The aluminum foil ($4{\times}12$ cm) coated with filtered juice of chinese cabbage leave (hereinafter called oviposition foil) exposed to 300 newly-emerged adults for 24 hours inside the rearing container. The oviposition foils were replaced every 4 days consecutively after mating, but it was better to discarded over then. Oviposition foil were divided into 6 to 12 pieces depending on egg mass volume. After dipping into test solutions for 10 seconds using faucet, oviposition foil pieces were placed into common petri dish, and then investigated hatchability. The effect of methanol solvent (50%) for 10 seconds dipping on the toxicity against DBM eggs was negligible. In addition, whether covering the petri dish or not should be dependent on nature of active compounds tested. With applying the new bioassay method, methanol extracts from 50 plants were tested the ovicidal activity to DBM eggs. Among them, four plant extracts; Angelica tenuissima root, Lycium chinense root, Cnidium officinale root and Polygala tenuifolia root, showed high ovicidal activity of over 90% control efficacy, against DBM eggs.

Larvicidal Activity of Constituents Identified in Piper nigrum L. Fruit Against the Diamondback moth, Plutella xylostella (후추 열매 유래 화합물들의 배추좀나방 유충에 대한 살충 활성)

  • Park, Il-Kwon
    • Korean journal of applied entomology
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    • v.51 no.2
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    • pp.149-152
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    • 2012
  • The larvicidal activities of Piper nigrum fruit methanol extracts and its constituents against larvae of the diamondback moth, Plutella xylostella, were investigated using the leaf dipping method. Administering the P. nigrum methanol extract resulted in 100 and 97% mortality against diamondback moth larvae at 5.0 and 2.5 mg/mL concentrations, respectively. Larvicidal activities of the P. nigrum fruit-derived piperidine alkaloids, piperine, and N-isobutylamide, as well as pellitorine, guineensine, pipercide, and retrofractamide A against P. xylostella varied according to test compound. Based on the 48 h $LC_{50}$ values, the most toxic compound to diamondback moth larvae was guineensine (0.013 mg/mL) followed by retrofractamide A (0.020mg/mL), pipercide (0.033mg/mL), and pellitorine (0.046 mg/mL). The $LC_{50}$ value of piperine was >0.5 mg/mL.