• Natural Product Sciences
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• v.12 no.1
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• pp.50-54
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• 2006

The wound healing activity of plumbagin and the chloroform extract of Plumbago rosea Linn. (Yoot), incorporated into ointments with yellow soft paraffin, have been investigated on rats. Wound healing activity was studied using excision and incision wound models in rats following topical application. Both plumbagin and the Plumbago rosea root extract produced a significant response in both of the wound models studied. The wound contracted in 14 days in the case of plumbagin (0.1%) and 16 days in case of Plumbago rosea root extract (0.5%), as against in 22 days in the case of control animals. The results were also comparable to those of a standard drug, framycetin sulphate cream (1% w/w) in terms of wound contracting ability, wound closure time, tensile strength of wound and regeneration of tissues at the wound site. Histological studies revealed evidences for the healing process by formation of fibrovascular tissue, epithelization and increased collagenization when compared to control.

• Journal of Plant Biotechnology
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• v.5 no.3
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• pp.181-185
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• 2003

Callus and suspension cultures derived from leaf explants of Plumbago rosea were established on Murashige and Skoog's medium containing 1 mg/L IAA, 0.5 mg/L NAA and 0.3 mg/L BAP. Callus cultures were tested for their growth and accumulation of plumbagin, a naphthoquinone and was identified by $^1H$ NMR and electron ionization mass spectroscopy. While auxins (not 2,4-D) influenced growth and plumbagin accumulation, cytokinins did not influence them much. Increasing concentrations of IAA in presence of NAA and BAP increased plumbagin in suspensions only up to 1 mg/L. Growth of callus was optimum (8.3 g DCW/I) at a hormonal combination of 1.5 mg/L IAA, 0.5 mg/L NAA and 0.3 mg/L BAP, but high plumbagin accumulation (4.9 mg/g DCW) was recorded at 1.0 mg/L IAA plus 0.3 mg/L BAP. Since instability in growth and secondary metabolite accumulation was noticed, several cell lines/clumps of callus were screened for plumbagin accumulation by visual and analytical methods. Biomass and accumulation of plumbagin showed a negative correlation in several cell lines. But one cell line showed stability both in terms of biomass and plumbagin accumulation over a period of 6 months.