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Histopathologic Changes of Articular Cartilage and Subchondral Bone in Cylindrical Biopsy Specimen from Talar Osteochondral Lesions (거골의 골연골 병변의 원주형 생검에서 관절 연골과 연골하 골의 조직병리학적 변화)

  • Lee, Ho-Seong;Jang, Jae-Suk;Lee, Jong-Suk;Cho, Kyung-Ja;Lee, Sang-Hoon;Jung, Hong-Keun;Kim, Yong-Min
    • Journal of Korean Foot and Ankle Society
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    • v.10 no.2
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    • pp.117-124
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    • 2006
  • Purpose: This study was aimed at elucidating the pathogenesis of talar osteochondral lesion by analyzing the histopathological findings. Materials and Methods: Twenty specimens from 20 patients who underwent surgical treatment for talus osteochondral lesions were studied. Preoperative MRI images including T1, T2, and stir images were taken and cases were classified according to modification of the Anderson's classification. There were 5 cases of MRI group 1, 6 cases of group 2, 7 cases of group 3 and 2 cases of group 4. A full thickness osteochondral plug including the osteochondral lesion of the talus was harvested from each patient and reviewed histopathologic changes of osteochondral fragment using H-E staining. Mean diameter of specimens was 8.5 mm and mean depth was 10.3 mm. Pathologic changes of articular cartilage and subchondral bone were observed. Subchondral bone was divided into superficial, middle and deep zones according to depth. Cartilage formation, trabecular thickening and marrow fibrosis were observed in each zone. Results: There were detachment of the joint cartilage at the tidemark in 16 cases of 20 cases and the separated cartilages were almost necrotic on the histopathologic findings. Cartilage formation within subchondral bone was discovered beneath the tidemark in 12 cases. Trabeculae were increased and thickened in 17 cases. These pathologic changes were similar to fracture healing process and these findings were more conspicuous near the tidemark and showed transition to normal bone marrow tissue with depth. No correlation between the pathological progression and MRI stages was found. A large cyst shown on MRI's was microscopically turned out to be multiple micro-cysts accompanied by fibrovascular structure and newly formed cartilage tissue. Conclusion: The histopathologic findings of osteochondral lesions are detachment of overlying cartilage at the tidemark and subsequent changes of subchondral bone. Subchondral bone changes are summarized as cartilage formation, marrow fibrosis and trabecular thickening that mean healing process following repeated micro fractures of trabecular. These osteochondral lesions should have differed from osteochondral fractures.

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The Spawning Behavior and Egg Development of Odontobutis interrupta IWATA and JEON, 1985 (얼룩동사리 (Odontobutis interrupta)의 산란행동 및 난발생에 관한 연구)

  • 최신석;나영언
    • Korean Journal of Environmental Biology
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    • v.18 no.3
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    • pp.323-330
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    • 2000
  • Early life history and spawning for Odontobutis interrupta were observed in the laboratory during May-August 1999 with a condition of natural habitats in the field. Optimal water temperature for spawning was between 17.5 and 22.$0^{\circ}C$ and appropriate water depth and current velocity in the natural habitat ranged 0.3-0.6 m and 0.1-0.3 m/sec, respectively. Ovary maturation index peaked at about 100mm in the total length and their values gradually decreased after the size. Male fishes showed a territory and courtship behavior to adult females and the males frequently pushed upper-ventral part of females for egg releases. After females spawned, the males guarded the egg masses and supplied dissolved oxygen using pectoral fins. According to observation of egg development in the laboratory, blastodisc formed in 1hr 17 min after the fertilization, cleavaging at 36-minute interval regularly. Blastulation occurred in 7 hr 12 min after the fertilization, with gastrulation after 11 hr 11 mins and formation of york plug after 32 hr 48 min. Embryo was formed in 33 hr 45 min after fertilization and optic vesicles appeared in 47 hr 27 mins when 30-31 somites were formed. Cardiac primordium was formed in 65 hr 15mins and its beat averaged 44- 48 time/min. Pectoral fins were formed in 138 hr 40 min, air-bladder and black vesicles were observed in lower portion of young fish. Embryo hatched from she-11 membrane after about 10 days and juvenile was 5.8$\pm$0.2mm in total length 3.0$\pm$0.5mg in weight.

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Study of Combustion Characteristics with Variations of Combustion Parameter in Ultra-Lean LPG Direct Injection Engine (연소제어인자의 변화에 따른 직접분사식 초희박 LPG엔진의 연소특성 연구)

  • Park, Yun Seo;Park, Cheol Woong;Oh, Seung Mook;Kim, Tae Young;Choi, Young;Lee, Yong Gyu
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.37 no.6
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    • pp.607-614
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    • 2013
  • Nowadays, automotive manufacturers have developed various technologies to improve fuel economy and reduce harmful emissions. The ultra-lean direct injection engine is a promising technology because it has the advantage of improving thermal efficiency through the deliberate control of fuel and ignition. This study aims to investigate the development of a spray-guided-type lean-burn LPG direct injection engine through the redesign of the combustion system. This engine uses a central-injection-type cylinder head in which the injector is installed adjacent to the spark plug. Fuel consumption and combustion stability were estimated depending on the ignition timing and injection timing at various air-fuel ratios. The optimal injection timing and ignition timing were based on the best fuel consumption and combustion stability.

Hearing Threshold Shift Level of Weavers on Wearing the Ear Plugs (이전장용여부(耳栓裝用與否)에 따른 난청도(難聽度)의 추이(推移))

  • Kim, Yeong-Hwan;Lee, Jong-Young;Kim, Doo-Hie
    • Journal of Preventive Medicine and Public Health
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    • v.17 no.1
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    • pp.57-63
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    • 1984
  • To assess the hearing conservation by the hearing protection devices in the field, 1,237 healthy female weavers of 16 to 24 year old were surveyed from April 1982 to January 1983. By reviewing the charts, their working durations, hearing threshold shift levels and whether wearing the ear plugs or not were checked. The hearing threshold levels were measured at 4,000Hz by audiometry AA-30A, RION Japan. They were divided into two groups: an experimental group with ear plug and the control group without it. The threshold shift level above 40dB in controls was 44.6% in left and 40.9% in right ear and that in experimental group 8.0% in left and 6.6% in right ear. The distribution of threshold shift levels between control and experimental group was significantly different in both ears, but that between left and right ear not significantly different in both groups. The longer the working durations were, the more the proportion of high threshold shift levels was and the larger the differences in the proportion of above 40dB between both groups were.

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Nano-scale Design of electrode materials for lithium rechargeable batteries

  • Gang, Gi-Seok
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2012.05a
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    • pp.72-72
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    • 2012
  • Lithium rechargeable batteries have been widely used as key power sources for portable devices for the last couple of decades. Their high energy density and power have allowed the proliferation of ever more complex portable devices such as cellular phones, laptops and PDA's. For larger scale applications, such as batteries in plug-in hybrid electric vehicles (PHEV) or power tools, higher standards of the battery, especially in term of the rate (power) capability and energy density, are required. In PHEV, the materials in the rechargeable battery must be able to charge and discharge (power capability) with sufficient speed to take advantage of regenerative braking and give the desirable power to accelerate the car. The driving mileage of the electric car is simply a function of the energy density of the batteries. Since the successful launch of recent Ni-MH (Nickel Metal Hydride)-based HEVs (Hybrid Electric Vehicles) in the market, there has been intense demand for the high power-capable Li battery with higher energy density and reduced cost to make HEV vehicles more efficient and reduce emissions. However, current Li rechargeable battery technology has to improve significantly to meet the requirements for HEV applications not to mention PHEV. In an effort to design and develop an advanced electrode material with high power and energy for Li rechargeable batteries, we approached to this in two different length scales - Atomic and Nano engineering of materials. In the atomic design of electrode materials, we have combined theoretical investigation using ab initio calculations with experimental realization. Based on fundamental understanding on Li diffusion, polaronic conduction, operating potential, electronic structure and atomic bonding nature of electrode materials by theoretical calculations, we could identify and define the problems of existing electrode materials, suggest possible strategy and experimentally improve the electrochemical property. This approach often leads to a design of completely new compounds with new crystal structures. In this seminar, I will talk about two examples of electrode material study under this approach; $LiNi_{0.5}Mn_{0.5}O_2$ based layered materials and olivine based multi-component systems. In the other scale of approach; nano engineering; the morphology of electrode materials are controlled in nano scales to explore new electrochemical properties arising from the limited length scales and nano scale electrode architecture. Power, energy and cycle stability are demonstrated to be sensitively affected by electrode architecture in nano scales. This part of story will be only given summarized in the talk.

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Pollen Tube Elongation and Fruit Set of Asian Pear (Pyrus pyrifolia) upon Mechanical Damage on the Style (동양배 암술의 기계적 손상에 따른 화분관 신장과 착과)

  • Kang, Sam-Seok;Kim, Yoon-Kyeong;Choi, Jang-Jeon;Cho, Kwang-Sik;Hwang, Hae-Sung;Jeong, Sang-Bouk;Lee, Hee-Jae
    • Horticultural Science & Technology
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    • v.28 no.6
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    • pp.942-947
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    • 2010
  • In the present study, pollen tube growth and fruit set in Asian pear ($Pyrus$ $pyrifolia$) were examined upon the mechanical damage on the stigma and style following the artificial pollination. When the upper middle part of style was cut off at 48 h after the artificial pollination, pollen tube grew to the middle of the style and the subsequent pollen tube elongation and fruit set normally occurred. When the style was cut off before the pollen tube reached the middle of the style, however, no pollen tube elongation and fruit set occurred. With the pollen tube elongation through the style, callose plugs at the basal part of the pollen tube were formed to the direction of the elongation at regular intervals. When the upper part of pollen tube attached pollen grain was cut off, however, callose plugs were formed defectively in the pollen tube, and the pollen tube opened and lost their turgor pressure. These facts might be the reason for the ceased or delayed pollen tube elongation and ultimately the failure of the fertilization.

Growth and Phytochemical Contents of Ice Plant as Affected by Light Quality in a Closed-type Plant Production System (완전제어형 식물생산시스템에서 광질에 따른 아이스플랜트의 생육과 기능성물질 함량)

  • Kim, Young Jin;Kim, Hye Min;Hwang, Seung Jae
    • Horticultural Science & Technology
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    • v.34 no.6
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    • pp.878-885
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    • 2016
  • A study was conducted to examine the effects of light quality on the growth and phytochemical contents of ice plant in a closed-type plant production system. Seeds were sown in a 128-cell plug tray using rockwool. The seedlings were then transplanted into a deep floating technique system with recirculating nutrient solution (EC $1.5dS{\cdot}m^{-1}$, pH 6.5) in a closed-type plant production system. The nutrient solution was supplied at two weeks after transplanting with 2.0 mM NaCl concentration in all treatments for the development of the bladder cells. The three light sources with different light qualities used were as followed; FL (fluorescent lamps), combined RW LED (red:white = 7:3), and combined RBW LED (red:blue:white = 8:1:1) at $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD with a photoperiod of 14/10 hours (light/dark). The results showed that the FL treatment had the greatest growth enhancement effects on the leaf area and the fresh and dry weights of the shoots and roots. The SPAD values were significantly higher under the FL and RBW LED treatments, at 29.8 and 30.6, respectively. No significant difference was observed in salinity under all treatments. Chlorophyll fluorescence was significantly higher under the FL treatment. The total phenol content and antioxidant activity were the highest under the RBW LED treatment. The total flavonoid content was significantly higher under the RBW LED and FL treatments. Hence, the results indicate that the growth of ice plant was maximized under the FL treatment. The phytochemical contents were maximized under the RBW LED treatment.

A Study of 3D Modeling of Compressed Urban LiDAR Data Using VRML (VRML을 이용한 도심지역 LiDAR 압축자료의 3차원 표현)

  • Jang, Young-Woon;Choi, Yun-Woong;Cho, Gi-Sung
    • Journal of Korean Society for Geospatial Information Science
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    • v.19 no.2
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    • pp.3-8
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    • 2011
  • Recently, the demand for enterprise for service map providing and portal site services of a 3D virtual city model for public users has been expanding. Also, accuracy of the data, transfer rate and the update for the update for the lapse of time emerge are considered as more impertant factors, by providing 3D information with the web or mobile devices. With the latest technology, we have seen various 3D data through the web. With the VRML progressing actively, because it can provide a virtual display of the world and all aspects of interaction with web. It offers installation of simple plug-in without extra cost on the web. LiDAR system can obtain spatial data easily and accurately, as supprted by numerous researches and applications. However, in general, LiDAR data is obtained in the form of an irregular point cloud. So, in case of using data without converting, high processor is needed for presenting 2D forms from point data composed of 3D data and the data increase. This study expresses urban LiDAR data in 3D, 2D raster data that was applied by compressing algorithm that was used for solving the problems of large storage space and processing. For expressing 3D, algorithm that converts compressed LiDAR data into code Suited to VRML was made. Finally, urban area was expressed in 3D with expressing ground and feature separately.

Expression of Growth Differentiation Factor-9 in the Mouse Ovaries at Different Developmental Stages (생쥐 난소의 발생단계에 따른 Growth Differentiation Factor-9의 유전자 발현)

  • 윤세진;이경아;고정재;차광열
    • Development and Reproduction
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    • v.3 no.1
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    • pp.95-100
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    • 1999
  • Growth differentiation factor-9 (GDF-9) is a member of the transforming growth factor $\beta$ (TGF-$\beta$) superfamily. It has been known that GDF-9 is a growth factor having a crucial role in normal folliculogenesis and its expression is oocyte-specific. The present study was aimed to elucidate the expression of GDF-9 mRNA in the mouse primordial follicles as well as in the other developmental stages. The semiquantitative analysis of GDF-9 mRNA expression was conducted. Total RNA was extracted from the ICR mice ovaries at gestational day 19, postnatal day 1, day 10, day 21, and day 28, and RT-PCR was performed to measure GDF-9 and $\beta$-actin mRNA levels. Level of GDF-9 mRNA were normalized against the level of $\beta$-actin mRNA, and compared among different stages. GDF-9 mRNA was detected in all samples including the fetal ovaries that mainly consists of primordial follicles. The highest level of mRNA was observed in ovaries obtained at day 10 that mainly consists of growing follicles. The present result suggests that GDF-9 may play an important role in the early stage of folliculogenesis.

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Seedling Conditions for Kimchi Cabbage, Head Lettuce, Cabbage and Broccoli for a Riding-type Transplanter

  • Lim, Jung-Hyun;Park, Suh-young;Chae, Won-Byung;Kim, Sung-Kyeom;Choi, Seung-Kook;Yang, Eun-Young;Lee, Min-Ji;Park, Gyeong-Bin;Jang, Yoon-ah;Seo, Myeong-Hoon;Jang, Suk-Woo
    • Journal of Biosystems Engineering
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    • v.42 no.2
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    • pp.104-111
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    • 2017
  • Purpose: We have studied the necessary qualities of seedlings of some leafy vegetables for a riding-type two-row automatic transplanter. When seedlings are planted using a transplanter, long roots may be rounded in the bottom of the tray, and this can interfere with the separation of the seedlings. Uprightness related to leaf spread angle is an important quality for seedlings in mechanical planting. Methods: To select cultivars suitable for the transplanter, we compared varieties of Kimchi cabbage (Chukwang, Daetong, Whipalam and Namdo), head lettuce (Abi and Sensation), cabbage (YR Onnuri, YR Hogel, Harutama, and Ogane), and broccoli (Nicegreen and Earlyyou). To compare the effect of bed soil on root formation and growth, we used five types of soil: Chologi, Burger, Wonjomix, Bio, and Baroker with 2.6-3 L per tray. Growth increment and the degree of root formation were measured according to the RDA guidelines 25 days after sowing for Kimchi cabbage and head lettuce and 44 days after sowing for cabbage and broccoli. Conclusions: According to the plug tray, the optimum seedling age in both 128- and 200-hole trays was 28 days for Kimchi cabbage and 44 days for cabbage and broccoli. Head lettuce took 35 days in 128-hole trays and 31 days in 200-hole trays. Burger soil was most effective for root formation and growth of the four kinds of leafy vegetables; it appeared that smaller soil volume led to faster root formation.