• Title/Summary/Keyword: Pleurotus cornucopiae(Per.) Rolland

Search Result 1, Processing Time 0.016 seconds

Synthesis of Resin Derivatives and Purification of Protein - Synthesis of Benzoyl-AH-Sepharose 4B and Purification in Pleurotus cornucopiae(mushroom) - (친화성 고분자 유도체의 합성 및 단백질의 분리정제에 관한 연구 - Benzoyl-AH-Sepharose 4B의 합성 및 흰느타리버섯중 단백질의 분리정제 -)

  • Min, Tae-Jin;Chang, Hung-Bae;Choi, Won-Ki
    • The Korean Journal of Mycology
    • /
    • v.16 no.3
    • /
    • pp.121-127
    • /
    • 1988

  • For selective purification of protein in Pleurotus cornucopiae (Per.) Rolland, affinity chromatography was performed by benzoyl-AH-Sepharose 4B gel synthesized using AH-Sepharose 4B with starting materials. Ligand capacity of benzoyl group was 9.3 micromole per milliliter of gel. Total apparent molecular weight of affinity protein was 255KD, which were protein complex of 29.5, 31.5 34.0, 71.0 and 89.0KD, respectively. The contents of nonpolar, polar, positively charged, and negatively charged amino acid were 45.68%, 26.93%, 11.81% and 15.58%, respectively. The nonpolar protein was selectively purified by hydrophobic ligand of benzoyl group of gel.

  • PDF