• KSBB Journal
• /
• 제15권2호
• /
• pp.162-166
• /
• 2000

도라지에 H2O, ethanol, ethyl ether와 petroleum ether를 용매로 하여 추출한 시료를 기관지질환 유발세균(S. aureus, C. diphtheriae, Mycobacterium sp. F. nucleatum, A. funicatus, K. pneumoniae, S. pyogenes N. gonorrhoeae)에 투여한 결과 ethyl ether, petroleum ether, H2O와 ethanol의 순으로 항균력이 우수하다는 것이 판명되었다. 특히, ethyl ether에 의한 도라지 추출물의 항균력이 80%이상 뛰어난 효과를 나타내었다. 우수한 항균력을 가지는 도라지 추출물의 농도는 30mg부터 30%이상의 억제작용을 보였으며 70mg에서 70%이상의 세포 성장이 저해되는 현상을 관찰하였다. 기관지 질환 유발세균에 대한 도라지의 효과는 대조구에 비해 70%이상의 탁월한 항균효과가 있는 것으로 확인되었다.

• 농촌계획
• /
• 제26권1호
• /
• pp.137-147
• /
• 2020

Rural Development Administration (RDA) has developed a manufacturing technology which can increase the amount of platycodin D in Platycodon grandiflorum. A study is needed to estimate the value of this new technology and predict the market demand for a new product. This study estimates additional amount of willingness to pay (WTP) using contingent valuation method (CVM) for a new product with the technology that RDA has developed. The survey was created under virtual health functional foods market of Platycodon grandiflorums. It was conducted with 1,000 adult males and females aged between 19 and 59 years old in 17 major cities and provinces nationwide from December 4 to December 7, 2018. The amount of WTP for the health functional Platycodon grandiflorum was drawn using maxinum likelihood estimation method. The estimated average and median WTP values are 21,933.85 won per person and 10,000 won per person, respectively. The independent factors, including the ratio of monthly average health function food consumption to income, the average monthly income level of a household, and existence of family members or relatives engaged in food and nutrition-related professions in a household, have been shown to have a statistically significant influence on the WTP. This study presents the potential magnitude of health functional food market and the value of the new technology based on health functional food market.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
• /
• pp.113.3-114
• /
• 2003

The increased deposition of extracellular matrix by hepatic stellate cells following liver injury in a process known as activation is considered a key mechanism for increased collagen content of liver during the development of liver fibrosis. In this study, we investigated the protective effects of an aqueous extract from the roots of Platycodon grandiflorum A. DC (Campanulaceae), Changkil (CK), on hepatic fibrosis in hepatic stellate cells. We report that CK reduces the accumulation of collagen in acetaldehyde-induced hepatic stellate cells. (omitted)

• Journal of Plant Biology
• /
• 제38권4호
• /
• pp.337-341
• /
• 1995

Hairy roots of Korean ballon flower (Platycodon grandiflorum A. DC) were induced from the root tissues infected with Agrobacterium rhizogenes ATCC 15834. Growth and polyacetylene [lobetyol (1), lobetyolin (2) and lobetyolinin (3)] production fo ten hairy root clones cultured in 1/4 Gamborg B5 (B5) liquid medium were determined. One selected hairy root clone (D6) grew well in hormone free-B5 liquid medium and showed maximum content of polyacetylenes at week 6 for 1 (0.375% dry wt) and at week 7 for 2 and 3 (3.030% and 0.206% dry wt, respectively) whose levels were much higher than those of the intact plant root (1:0.019%, 2:0.077% dry wt, 3 was not detected).

• 한국식품과학회지
• /
• 제46권6호
• /
• pp.769-772
• /
• 2014

도라지 건조 온도에 대한 기본 정보를 제공하고자 건조 온도에 따른 환원당 그리고 사포닌 함량을 조사한 결과를 요약하면 아래와 같다. Sucrose 함량은 $85^{\circ}C$에서 3,825 mg% $45^{\circ}C$에서 1,226 mg%로 건조 온도가 낮을수록 감소하였지만, glucose함량은 건조 온도가 높을수록 감소하였다. 본 시험에서 분석한 도라지 4종의 사포닌 중에 platycodin D 함량은 $85^{\circ}C$에서 197.5 mg%, $65^{\circ}C$에서는 334.7 mg%로 $45^{\circ}C$에서 956.0 mg%로 가장 높게 나타나 건조 온도가 낮을수록 증가하였으며, 고온(85) 건조에서 사포닌 중 polygalacin D의 함량이 가장 많았다. Sucrose와 사포닌 함량 간의 상관계수는 각각$-0.987^{**}$로 고도의 음의 상관을 보였다(p<0.01). 건조 온도 $45^{\circ}C$가 다른 건조 온도에 비해 색이 밝게 나타남을 확인할 수 있었다. 이상의 결과를 통하여 도라지의 건조 온도가 도라지의 사포닌 함량과 당 함량에 영향을 미치는 것을 확인할 수 있었으며, 도라지의 이용 용도에 따라 건조 온도를 달리 할 필요성이 있을 것으로 사료된다.

• 한국약용작물학회지
• /
• 제22권6호
• /
• pp.463-468
• /
• 2014

This study was carried out in order to survey the soil physico-chemical, morphology and content of saponins of 5 year-old Platycodon grandiflorum radix from different areas in Gyeongnam. Soil physical properties was showed 1.01-1.29 of bulk density of soil and soil hardness was increased with increasing soil depth, especially below the 30 cm, however it was maintained or small increased below the 40 cm. T-N and OM contents of top soil were highest at Geochang as 0.27% and 56.9 g/kg, respectively, compared to other areas. The root length was longer at Kimhea and Geochang as 33.8 and 33.7 cm, respectively, and fresh weight was heavy at Geochang as 208.5 g. The contents of saponin of P. grandiflorum radix was higher in fine root compare to main root. By region, content of saponins of P. grandiflorum radix were higher at Hapcheon than other area. Mg content was highly negatively correlated at p < 0.01 with platycodin D3, deapioplatycodin D, platycodin D, and deapioplatycodin D ($-0.499^{**}$, $-0.433^{**}$, $-0.421^{**}$, $-0.511^{**}$ respectively). These results suggest that no-fertilized Mg effected on the improvement of saponin contents in P. grandiflorum.

• 생약학회지
• /
• 제54권1호
• /
• pp.44-51
• /
• 2023

Fine dust has emerged as seriously hazardrous particles, which can cause respiratory and cardiovascular diseases. Thus, we have developed a natural complex extract consisted of Codonopsis lanceolata (Siebold & Zucc.) Trautv., Platycodon grandiflorum A. De Candolle, and Glycyrrhiza uralensis Fischer in the ratio of 3 : 5 : 2, which have a beneficial effect on respiratory system. In this study, simultaneous quantitative analysis of marker compounds, lobetyolin, platycodin D, and glycyrrhizic acid in the natural complex extract was developed and validated with high performance liquid chromatography-photodiode array detector. The marker compounds were shown in a large linearity with a correlation coefficient (R²) of 1.000. The limit of detection (LOD) of lobetyolin, platycodin D and glycyrrhizic acid were 5.40 ㎍/mL, 3.94 ㎍/mL and 5.86 ㎍/mL, respectively. The limit of quantification (LOQ) of lobetyolin, platycodin D and glycyrrhizic acid were 16.36 ㎍/mL, 11.94 ㎍/mL and 17.75 ㎍/mL, respectively. The content analysis revealed that the amounts of lobetyolin, platycodin D, and glycyrrhizic acid were 0.039±0.013 mg/g, 0.337±0.048 mg/g, and 1.171±0.003 mg/g, respectively, in the natural complex extract. Therefore, these results could be used as basic data for the standardization and quality control of the natural complex extract.

• 한국약용작물학회지
• /
• 제23권5호
• /
• pp.363-369
• /
• 2015

Background : Platycodon grandiflorum radix used as a therapeutic agent for lung and respiratory diseases in traditional Korean medicine. The saponin and sugar content in P. grandiflorum root is dependent on the drying temperature and method. In this study, we investigated saponins and antioxidant contents in dried P. grandiflorum roots either not cut or cut to different lengths. Methods and Results : P. grandiflorum roots were either cut to 0.5, 1.0 or 2.0 cm in length or left uncut. They were dried at $45^{\circ}C$ and the effect of antioxidant substances, antioxidant ability, and saponin content were investigated. P. grandiflorum roots rapidly dried out when cut to short lengths. The saponin contents (platycodin D, polygalacin D, and deapioplatycodin D) was highest in the uncut P. grandiflorum roots. When the cut lengths were long, the levels of polyphenolic compound increased. However, 2,2'-azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were higher in the samples cut to shorter lengths. Conclusions : These results suggested that the saponin and antioxidant content of P. grandiflorum roots could be maximized by controlling the drying rate of the roots via adjustments to the root cutting length.

• 한국식품과학회지
• /
• 제46권5호
• /
• pp.636-640
• /
• 2014

도라지 건조방법에 대한 기본 정보를 제공하고자 일광건조, 열풍건조 그리고 동결건조를 실시하여 무기성분과 환원당 그리고 사포닌 함량을 조사한 결과를 요약하면 아래와 같다. 칼륨과 칼슘은 각각 22.6과 9.2 mg%로 열풍건조에서 높게 나타났다. Platycodin D는 열풍건조에서 622.0mg%로 가장 높게 나타났으며, platycodin D3, Polygalacin D, deapioplatycodin D는 일광건조에서 각각 113.5, 756.6, 109.2 mg%로 나타났다. Glucose와 platycodin D, platycodin D3, deapioplatycodin D의 함량간의 상관계수는 각각 -0.924, -0.957, -0.861로 고도의 음의 상관을 보였다(p<0.01). 동결건조가 다른 건조조건에 비해 색이 밝게 나타났다. 이상의 결과로 도라지의 건조 방법은 일광건조와 열풍건조가 동결건조보다 좋은 것으로 평가 되었지만 일광건조는 건조기간이 길어 많은 노동력이 소요된다. 따라서 열을 가하여 건조하면서 건조기간도 줄이고 도라지 약리성도 높이기 위한 적정 건조 온도에 관한 연구도 수행 되어야 할 것으로 사료된다.

• Nutrition Research and Practice
• /
• 제14권5호
• /
• pp.453-462
• /
• 2020

BACKGROUND/OBJECTIVES: Platycodon grandiflorum (PG), an oriental herbal medicine, has been known to improve liver function, and has both anti-inflammatory and antimicrobial properties. However, little is known about the immune-enhancing effects of PG and its mechanism. In this study, we aimed to investigate whether fermented PG extract (FPGE), which has increased platycodin D content, activates the immune response in a murine macrophage cell line, RAW 264.7. MATERIALS/METHODS: Cell viability was determined by Cell Counting Kit-8 assay and the nitric oxide (NO) levels were measured using Griess reagent. Cytokine messenger RNA levels of were monitored by quantitative reverse transcription polymerase chain reaction. To investigate the molecular mechanisms underlying immunomodulatory actions of FPGE in RAW 264.7 cells, we have conducted luciferase reporter gene assay and western blotting. RESULTS: We found that FPGE treatment induced macrophage cell proliferation in a dose-dependent manner. FPGE also modulated the expression of NO and pro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin (IL)-1β, and IL-6. The activation and phosphorylation levels of nuclear factor kappa B (NF-κB) were increased by FPGE treatment. Moreover, 5-aminoimidazole-4-carboxamide ribonucleotide, an activator of AMP-activated kinase (AMPK), significantly reduced both lipopolysaccharides- and FPGE-induced NF-κB reporter gene activity. CONCLUSIONS: Taken together, our findings suggest that FPGE may be a novel immune-enhancing agent acting via AMPK-NF-κB signaling pathway.