• 한국환경과학회지
• /
• 제18권1호
• /
• pp.15-19
• /
• 2009

Early studies claimed that heterotrophic dinoflagellates Pfiesteria piscicida and related genera may produce a putative water-soluble toxin that causes death of fish and other marine animals. Several methods were tested to visualize plate morphology of Cryptoperidiniopsis brodyi and Pfiesteria piscicida. Cellulose plates of cells were exposed and visualized- by a membrane stripping method using Triton X-100. While calcofluor M2R white stain could readily bind to the thecal plates, details of the plate tabulation were difficult to observe. Fixation with osmium tetroxide $(OsO_4)$ produced well preserved cells with little morphological distortion, but thecal plates could not be visualized. Scanning electron microscopy (SEM) observation using the membrane stripping method showed distinctive plate tabulations between C. brodyi and P. piscicida suggesting that this method is a useful tool for morphological identification of lightly armored dinoflagellates.

• Structural Engineering and Mechanics
• /
• 제86권1호
• /
• pp.69-83
• /
• 2023

Analytical solutions to problems are crucial because they provide high-quality comparison data for assessing the accuracy of numerical solutions. Benchmark analytical solutions for the vibrations of cracked functionally graded material (FGM) plates are not available in the literature because of the high level of complexity of such solutions. On the basis of first-order shear deformation plate theory (FSDT), this study proposes analytical series solutions for the vibrations of FGM rectangular plates with side or internal cracks parallel to an edge of the plates by using Fourier cosine series and the domain decomposition technique. The distributions of FGM properties along the thickness direction are assumed to follow a simple power law. The proposed analytical series solutions are validated by performing comprehensive convergence studies on the vibration frequencies of cracked square plates with various crack lengths and under various boundary condition combinations and by performing comparisons with published results based on various plate theories and the theory of three-dimensional elasticity. The results reveal that the proposed solutions are in excellent agreement with literature results obtained using the Ritz method on the basis of FSDT. The paper also presents tabulations of the first six nondimensional frequencies of cracked rectangular Al/Al2O3 FGM plates with various aspect ratios, thickness-to-width ratios, crack lengths, and FGM power law indices under six boundary condition combinations, the tabulated frequencies can serve as benchmark data for assessing the accuracy of numerical approaches based on FSDT.