• Research in Plant Disease
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• v.12 no.3
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• pp.260-266
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• 2006

Pythium blight caused by Pythium spp. is one of major diseases in putting green of golf course. In this study, microorganisms which are anatgonistic to Pythium aphanidermatum, a pathogen of pythium blight, were selected primary through in vitro tests, dual culture method and triple layer agar diffusion method. In vivo test against pythium blight were conducted to select the best candidate biocontrol microorganism by pot experiment in a plastic house. Bacillus subtilis GB-0365 was finally selected as a biocontrol agent against pythium blight. Relative Performance Indies(RPI) was used as a criterion of selecting potential biocontrol agent. B. subtilis GB-0365 showed resistance to major synthetic agrochemicals used in golf course. Alternative application of synthetic agrochemicals and B. subtilis GB-0365 was most effective to successfully contol pythium blight. B. subtilis GB-0365 suppressed the development of pythium bight of bentgrass by 56.4% as compared to non-treated control and its disease control efficacy was 60.9% of a synthetic fungicide Oxapro(WP) efficacy. B. subtilis GB-0365 has a potential to be a biocontrol agent for control of pythium blight.

• Journal of Bio-Environment Control
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• v.14 no.3
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• pp.182-189
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• 2005

This research was conducted to determine physical properties of four root media, peatmoss + vermiculite (1:1, v/v; PV), peatmoss + composted rice hall (1:1, PR), peatmoss + composted saw-dust (1:1 : PS) and peatmoss + composted pine bark (1:1 PB), as influenced by incorporation rate of Stock-sorb C (STSB). Each root medium containing STSB was packed in 22 cm diameter plastic pot and the physical properties were determined at 5 weeks after packing. As incorporation rate of STSB were elevated, total porosity increased in PV, PS and PB media with statistical differences at $5{\%}$ level. Those also resulted in increase of container capacity in PS and PB media, but statistical differences were not observed in PV and PR media. Elevated incorporation rate of STSB in PV, PS and PB media resulted in increase of air space with statistical differences. Trends in air space of the three root media showed a linear as well as quadratic responses to STSB contents of media. As incorporation rate of STSB increased, more water was retained in four root media at the soil moisture tension of 4.90 kPa, 9.81 kPa, 29.4 kPa and 1.5 MPa. The amount of water retained in PS medium was the highest at the moisture tension at 29.4 kPa and 1.5 MPa followed by PB, PR and PV medium. These results indicated that elevation of incorporation rate of STSB to various root media increased moisture retention capacity, but did not increase the available water holding capacity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.5
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• pp.480-488
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• 1994

Low seedling growth rates of white clover (Trifolium repens L.) have been limited its good establishment to pastures. The experiment was done to determine the effect of removal of cotyledon and unifoliolate on the growth and morphological characters of contrasting white clover cultivars for 8 weeks after the treatment. Individual plants of cv. Regal (large leaf), Louisiana S-l (medium-large leaf), Grasslands Huia (medium-small leaf) and Aberystwyth S184 (small leaf) were grown in 10cm plastic pot containing a 2:1:1 soil:sand:peat moss mixture until the cotyledon or unifoliolate stage and then removed one (C1) or two cotyledons (C2) at cotyledon stage, and unifoliolate only (U), unifoliolate and one cotyledon (DC1) or unifoliolate and two cotyledons (DC2) at the unifoliolate stage. To measure the removal effect on biomass and morphological characters (leaf area, petiole and stolon lengths, growing tips and leaves), plants were sampled 4, 6 and 8 weeks after the treatment. Intact plants had greater biomass and morphological characters than removal-treated ones, Removal treatments at cotyledon stage, C1 and C2, were decreased more biomass and morphological characters than removal ones at unifoliolate stage while the severer cotyledon removal, the more reduction. Stolon length per plant and petiole length markedly inclined 6 weeks after the treatments although biomass and the other characters continuously did. Relatively large-leaved cultivar had more biomass, leaf area per plant and longer petiole than the other(s) but the reverse results were true in stolon length, growing tips and no. of leaves per plant. Biomass was linearly increased with increased leaf area but the earlier and severer removal, the less slope. The severer damage of cotyledon and unifoliolate had detrimental effects on the growth and aftermath establishment of white clover

• Weed & Turfgrass Science
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• v.5 no.4
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• pp.187-190
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• 2016

This study was conducted to determine phytotoxicity of domestic rice varieties and characteristic of sensitive gene involved in herbicide reaction of 4-HPPD (4-hydroxy phenylpyruvate dioxygenase) inhibiting herbicides. The five rice varieties were grown for 2 to 3 leaf stage on seedling trays and then transplanted into plastic pot: 4-Japonica type (Sangjubyeo, Sambaekbyeo, Sanduljinmi and Kumyoung) varieties and 1-Indica type variety (IR8). We trialled standard (14 g a.i. $10a^{-1}$) and double fold (28 g a.i. $10a^{-1}$) dose of benzobicyclon treatment at 10 days after transplanting in order to investigate phytoxicity. The Japonica-type Sangjubyeo showed no rice injury but Indica-type IR8 show 4-5 (standard) and 5-6 (double fold) phytoxicity levels. In spite of Japonica-type, Sanduljinmi and kumyoung showed 3-4 and 4-5 levels. Target resistant gene, Hydroxyphenylpyruvate dioxygenase inhibitor sensitive gene No.1 (HIS1) have been isolated from five domestic rice cultivars (Sangjubyeo, Sambaekbyeo, Sanduljinmi, Kumyoung and IR8). Results of the sequence through PCR, all five tested rice cultivars had HIS1 gene regardless to rice cultivars. And the difference between rice varieties from sequence of HIS1 were identified some variation in genes.

• Journal of the Korean Society of Food Culture
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• v.15 no.2
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• pp.147-153
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• 2000

A survey on the preferences and notion about kimchi and kimchi products was conducted from july to october, 1999 to investigate basic information for increasing the consumption of kimchi for sale. 1,241 subjects of women and men aged 10 to 60 in Kyung-nam area participated in this survey. Most of the subjects made kimchi by themselves at home but this rate of making kimchi at home became lower than past, so increment of consumption of kimchi for sale will be expected in the future. When the people buy kimchi, they considered taste first, and then hygiene, nutrition in order. When they purchase kimchi, more men (69.1%) than women(56%) considered taste, but more women(36.1%) than men(20.6%) considered hygiene first. Subjects aged 30 or older groups considered more the hygiene of kimchi than the subjects of $10{\sim}20$ age groups did.(p<0.05) And people of household income over 3 million wons considered more hygiene than people of below 1 million wons income group did. People liked manufactured kimchi the packed with whole or partly transparent material in order to observe the contents.(p<0.05) They liked better kimchi packed with bottle(46.1%) and vinyl(39.6%) than plastic(14.3%) and this tendency was more in the subjects of women(49.8%) and over 30 age groups.(p<0.05) They prefered $200{\sim}500g$ packing unit of kimchi whether the family size were big or small. Among the processed kimchi products, men liked better a rice covered up with kimchi, a pot stew with kimchi or a soup with kimchi, On the other hand, women liked better a dumplings with kimchi, a grilled food with kimchi, a pizza with kimchi.(p<0.05) Subjects aged 10 to 20 years old liked all kinds of kimchi products, but people aged 40 or older disliked them.(p<0.001)

• Journal of Bio-Environment Control
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• v.11 no.3
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• pp.127-132
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• 2002

Enhanced supply of $Ca^{2＋}$ as well as NO$_3$$^{[-10]}$ is known to restrict the uptake of the Na$^{＋}$ and Cl$^{[-10]}$ ion and ameliorate growth under saline conditions. This test was conducted to investigate the ameliorating effects of Ca(NO$_3$)$_2$ or CaCl$_2$ on the growth and yield of NaCl-stressed tomato plants grown in plastic pot filled with soil. All treatments except for the control were supplied with 80 mM NaCl fur two weeks after transporting. The saline solutions with nutrient were supplemented with either 0, 10 or 20 mM Ca(NO$_3$)$_2$ and either 0, 10 or 20 mM CaCl$_2$ during harvesting time from two weeks after transporting. Ca(NO$_3$)$_2$ or CaCl$_2$ application enhanced the growth such as plant height, fresh weight, dry weight, fruit number, and fruit weight, and yield of NaCl-stressed tomato, and also their effects increased greater as concentration of supplemented Ca(NO$_3$)$_2$ or CaCl$_2$increased. Yield increased in 20 mM Ca(NO$_3$)$_2$ compared with the others except fur the control. Photosynthetic rate in Ca treatments was lower than that of the control, but higher than that of NaCl treatment. Leaf chlorophyll content was higher in Ca treatments compared with the others, especially in younger leaf, while that was not affected by concentration of supplemented Ca. Ca(NO$_3$)$_2$ or CaCl$_2$ supply increased the $K^{＋}$ and $C^{2＋}$ concentration of tomato plants, whereas the Na$^{＋}$ transport to the leaves was inhibited. There was a strong increase in the $K^{＋}$/Na$^{＋}$ ratio in plants treated Ca(NO$_3$)$_2$, or CaCl$_2$. Cl$^{[-10]}$ content of plants was decreased by supplemental Ca(NO$_3$)$_2$ but Cl$^{[-10]}$ was increased in plants with CaCl$_2$compared with Ca(NO$_3$)$_2$. N concentration in plants of tomato increased with enhanced Ca(NO$_3$)$_2$ or CaCl$_2$supply, In conclusion, our study confirms the potential of Ca(NO$_3$)$_2$ or CaCl$_2$to alleviate NaCl-induced growth reductions in tomato.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.2
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• pp.83-90
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• 2004

A pot experiment was conducted to find out the effects of nitrogen content of irrigation water and soil EC on lettuce growth under plastic film house conditions. The square-pots with 42 x 54.5 x 22 cm in length, width and height, were filled with two different soils of different EC. Lettuce was grown with different nitrogen fertilizer application levels including non fertilization (Non-F), decrement of 50% of nitrogen fertilizer recommended by soil testing (DNFRST-50) and fertilization recommended by soil testing (FRST). Two kinds of irrigation water of different nitrogen contents, $6.6mg\;L^{-1}$ and $21.0mg\;L^{-1}$, were used for the experiment. In the low EC soil irrigated with low nitrogen water, fresh weights of lettuce were 6,733, 11,933 and $12,733kg\;ha^{-1}$ for the treatments of Non-F, DNFRST-50 and FRST, respectively. While with high nitrogen water, the yields were 9,733, 13,400 and $12,800kg\;ha^{-1}$, respectively. In the high EC soil irrigated with low nitrogen water, lettuce yields of the Non-F, DNFRST-50 and FRST treatments were 12,400, 12,867 and $10,400kg\;ha^{-1}$, respectively, and with high nitrogen irrigated water lettuce yields of the Non-F, DNFRST-50 and FRST treatments were 13,600, 14,067 and $10,733kg\;ha^{-1}$, respectively. Nitrogen uptake of lettuce from ferilizer in DNFRST-50 was higher than of FRST. Nitrogen uptake of lettuce from irrgation water was found in soils of low EC, but it was not found in soils of high EC. These results suggest that both soil EC and nitrogen content of irrigation water should be considered when we recommend the level of fertilizer application for lettuce.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.2
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• pp.195-202
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• 1995

This experiment was performed to characterize the optimum water table level for the growth and yield of barley(var. Olbori) and wheat(var, Grumil), Olbori and Grumil were grown in the 550 liter plastic pot filled with silt loam or sandy loam, During the whole growth period, the water table adjusted to be 20, 30, 40, 50, and 70cm, Higher water table was resulted in the decrease in plant height and top dry weight, but in the increase of the ratio of top to root dry weight, especially in barley, This suggested that high water table level affected more the growth of top than that of root, The number and area of green leaves were decreased as the water table was higher than 30 to 40cm at the late growth period(May 18, 1993), The largest number and area of green leaves were shown at 50cm of water table in sandy loam and at 70cm in silt loam, As the water table was high, the leaf chlorophyll content was low, And barley was affected more significantly than wheat by soil texture, The photosynthetic activity was decreased remarkably at 20cm water table, Heading period was 2 to 3 and 4 days earlier at the 20cm water table of sandy loam in barley and wheat, respectively, However this earlier heading was not shown in silt loam, Grain filling was accelerated 5 to 7 days earlier in barley and 10 days in wheat grown at 20cm water table, The highest yield was present at 50 and 70cm water table, The yield was decreased remarkably at 20cm water table, resulting that yield reduction ratio of barley was 71.1% and 72, 2%, and that of wheat was 41.0% and 60, 0% in sandy loam and silt loam, respectively, High water table decreased the number of spike per unit area, but increased the seed weight per spike in barley, However, High water table reduced the seed weight per spike in wheat. There was significant correlation between yield and leaf chlorophyll content in wheat and barley, Yield was correlated significantly with green leaf area in barley, and with top dry weight, ratio of top to root dry weight chlorophyll content and photosynthetic activity in wheat. The optimum water table was 50 to 70cm in wheat and barley, They grew fairly well at 30cm water table of sandy loam, and at 40cm of silt loam.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.26 no.2
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• pp.125-132
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• 1990

The authors carried out an experiment to investigate the responsive behavior of sea eel, Astroconger myriaster(BREVOORT) to chemical stimuli. The experimental tank was made in doughnut type by using FRP plates. The channel of tank was divided into three concentric troughs by using perforated plastic plates. The inside trough was used as influent part, the outside one as effluent part, and the middle one as the testing trough in which testing fish may be swimmable. The influent part was radially portioned into 12 sections so as to be 30 degrees of central angle. But a basin of any section in testing trough was diverged in the range of 45 degrees of central angle. The the testing trough are radially divided into eight zones. Water is supplied at the rate of 6.3l per minute from the central water tank set as high as 50cm in the center of doughnut, passed across the influent part, testing trough, effluent part and finally discharged by overflow pipes. The chemical substance to stimulate the sea eel was extracted from mackerel, shad and krill which are used as bait for fishing. The chemical substance was injected into any one of 12 hoses which supplies water from central water tank to the influent part at the rate of 2ml per minute. Sea eels used for the experiment were caught by pot in the coast of Chung-mu and accustomed to the tanks for 5 days before applying them to the experiment. The result obtained are as follows: 1. The rate of time length of sea eel's staying in the stimulated zone, as the extracts of mackerel, shad and krill were given to it, was observed as 7.9%, 30.9% and 11.4% respectively. It means that the extract from shad was the most effective of three in attracting sea eel. 2. To compare the effect of freshness of bait fish, the extracts were prepared from shad just killed, form the 24 hour-lapsed one and from the 48 hour-lasped one after killed. The rate of time length of sea eel's staying in the stimulated zone was 30.9%, 17.1% and 11.3% respectively. It means that the freshness is much effective in attracting olfactory fishes like sea eel.

• Horticultural Science & Technology
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• v.33 no.2
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• pp.219-226
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• 2015

The objective of this research was to investigate the influence of various root media on the growth of mother and daughter plants during propagation and early yield after transplanting of 'Seolhyang' strawberry. To achieve this, daughter plants were fixed to connected small pots that contained expanded rice-hull (ERH), a strawberry-specialized commercial medium (SSCM), soil mother materials (SMM), or loamy sand (LS). Then, growth of daughter plants in above- and below-ground tissue as well as early yield after transplanting to plastic house soil were investigated. The growth of daughter plants in terms of plant height, leaf area and fresh weight were the highest in the SSCM treatment. Root growth in terms of the amount of primary roots and root dry weight were the highest in the treatments of ERH and SMM and the lowest in that of SSCM, among treatments tested. The ERH treatment also showed the highest values among treatments in root length, surface area and volume when roots with 0 to 0.4 mm in diameter were investigated. The flower bud differentiation of daughter plants began on Sept. 3 in the ERH treatments, earlier than the SMM (Sept. 5) and in SSCM (Sept. 7) treatments. The tissue N contents of daughter plants were in the range of 1.41 to 1.55% in all treatments, and no significant differences were observed among treatments. This indicates that the low moisture retention capacity of ERH and water stress, rather than tissue N contents, promote the flower differentiation of daughter plants. In the evaluation of early yield after transplant, the ERH treatment of showed the highest yield in the period from November to December, reaching 667 g fruit weight per 10 plants. The yields per 10 plants in the other treatments were 581 g in SMM, 475 g in SSCM and 295 g in LS. Above results imply that the various root media have different effects on the growth of daughter plants as well as flower bud differentiation. Therefore, improvement in early yield after transplant can be achieved through selection of proper root medium for daughter plant propagation.