• Journal of Plant Biotechnology
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• 제31권3호
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• pp.209-217
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• 2004

기내 배양환경을 조절하여 건전한 유식물체를 획득하기 위해 도라지를 무균발아시켜 shoot부분을 NAA 0.1 mg/L가 첨가된 MS 기본배지에 치상한 후 membrane filter 부착 유무와 sucrose 농도를 달리 처리하여 생장반응을 조사하였다. Membrane filter 미부착구에서는 배양용기 내의 $CO_2$와 $C_2$H$_4$ 농도가 membrane filter 부착구에 비해 높았으며 membrane filter가 부착되지 않았을 때 $CO_2$ 농도는 배양기간이 경과될수록 증가하였으나 $C_2$H$_4$ 농도는 감소하는 경향이었다. 식물체 생장은 membrane filter 부착구가 미부착구에 비해 양호하였고, 미부착구에서는 sucrose 농도가 증가할수록 생육이 양호하게 나타났다. 생체중과 건물중은 membrane filter를 부착하지 않은 sucrose 4.5% 처리구에 비해 membrane filter를 부착한 sucrose 무첨가구에서 비슷하거나 높았다. 엽록소 함량은 membrane filter 부착구에서 미부착구에 비해 높았으며 당 함량도 비슷한 경향이었는데 두 처리간 차이는 더 컸다. 기공은 membrane filter 부착구에서 미부착구에 비해 닫혀 있었으며 엽육조직은 membrane filter를 부착하거나 sucrose 농도가 높을수록 잘 발달하였다. 또한 상토에 이식한 후 13일째 생존율은 sucrose 처리 농도에 관계없이 membrane filter 미부착구는 0%인데 비해 부착구는 100%로 나타났다.

• 생물환경조절학회지
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• 제18권1호
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• pp.23-28
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• 2009

이전 실험에서 결정된 생육 단계별 최적 환경조건을 평가하기 위한 4가지 처리는 다음과 같았다: 생육 단계별 최적 환경 조건을 사용한 광독립 영양배양(photoautotrophic optimum condition with growth stage (POG)), 생육 단계별 평균 광합성 광량자속 밀도(photosynthetic photon flux density(PPFD))와 $CO_2$ 농도를 사용한 광독립 영양배양(photoautotrophic constant condition with average PPFD and $CO_2$ of POG(PCA)), 생육 단계별 최대 PPFD와 $CO_2$농도를 사용한 광독립 영양배양(photoautotrophic constant condition with maximum PPFD and $CO_2$ of POG(PCM)) 그리고 대조군으로 3%의 당을 포함한 광혼합 영양배양(photomixotrophic conventional condition with 3% sucrose(PMC)). 실험 결과 각 생육 단계별 환경제어(POG)는 기내에서 배양된 감자 소식물체의 모든 생육 관련 항목에서 유의적 증진을 유도하였다. 또한 단위 건물중 당 소비된 전력과 $CO_2$는 모든 처리 중 POG에서 가장 낮았다. 기외 이식 이후에도 POG에서 생산된 감자 묘는 PMC에서 자란 감자 묘와 전체적으로 큰 차이 없이 왕성한 생육을 유지하였다. 특히 POC는 기존 광혼합 영양방식(PCM)과 비교했을 때 기외 이식전과 이식 후 20일째 각각 4.7배와 3.8배 높은 건물중을 기록하였다. 따라서 POG와 같은 생육 단계별 환경 조절을 통한 광독립 영양 미세 증식 방법은 에너지 절감 효과와 함께 무균의 건강한 감자 묘의 생산에 효과적이었다.

• 원예과학기술지
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• 제33권4호
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• pp.585-590
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• 2015

본 실험은 생물반응기를 이용한 딸기 조직배양묘 대량증식 시 flurprimidol 을 처리하여 순화율을 향상시키고자 실시하였다. Flurprimidol 을 0, 0.1, 0.5, 1.0 및 $2.0mg{\cdot}L^{-1}$의 농도로 6주동안 처리하여 생육조사한 결과, flurprimidol을 첨가한 처리구의 생장량이 대조에 비해 감소하였고, 처리농도가 증가할수록 생장억제효과가 크게 나타났다. 초장은 대조가 7.9cm인 것에 비해 flurprimidol 처리는 2.2-3.7cm 범위로 매우 짧아졌다. 뿌리수는 대조가 51.8개/주에 비해 flurprimidol 처리가 11.6-34.2개/주 범위로 감소하였다. 뿌리길이는 대조가 4.36cm에 비해 flurprimidol 처리는 0.88-3.08cm 범위로 크게 짧아졌다. 그러나 액아수는 flurprimidol $2.0mg{\cdot}L^{-1}$ 처리를 제외하고 모든 처리구에서 대조의 8.6개/주에 비해 많이 발생하였을 뿐만 아니라 액아수가 증가할수록 그에 따라 잎수도 증가하였다. Flurprimidol $1.0mg{\cdot}L^{-1}$ 처리 시 뿌리가 부패하여 지하부 발달이 불량하였다. Flurprimidol $1.0mg{\cdot}L^{-1}$와 $2.0mg{\cdot}L^{-1}$ 처리를 제외한 모든 처리의 순화율은 100%로 대조에 비해 23.3% 향상되었다. 순화 4주 후, flurprimidol $0.1mg{\cdot}L^{-1}$ 처리는 생체중을 제외한 지상부 및 지하부 생육이 대조와 비슷하게 회복되었다. 따라서 생물반응기를 이용한 딸기의 조직배양묘 대량 증식 시 순화율 향상을 위한 적정 flurprimidol 농도는 $0.1mg{\cdot}L^{-1}$였다.

• Journal of Plant Biotechnology
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• 제4권4호
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• pp.179-181
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• 2002

Nodal explants of Ocimum basilicum L. (Sweet basil, Lamiaceae), showed shoot proliferation after 7-10 days on MS media containing 1.5 mg/L kinetin. In vitro flowering was achieved from 90% of the shootlets which were sub cultured on a half strength MS media fortified with 5 mg/L BAP and 1 mg/L IAA. Cytokinin alone or in combination with $CA_3$and NAA resulted in shoot proliferation only. For rooting the plantlets were subcultured on MS basal medium supplemented with 3 mg/L NAA and rootlets emerged after 10 days of incubation. The survival percentage of transplanted plantlets was 70%.

• Journal of Plant Biotechnology
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• 제34권3호
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• pp.197-200
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• 2007

Mature seeds of Artemisia annua L. were placed onto Murashige and Skoog's (MS) medium supplemented with $4.52\;{\mu}M$ 2,4-dichlorophenoxyacetic acid (2,4-D). After 6 weeks of culture, off-white, compact calluses were formed on the plumule of seedlings at a frequency of 5.9%. Calluses were subcultured on the same medium. After an additional 2 weeks of subculture, calluses produced a few somatic embryos at a frequency of 28.8%. Upon transfer to MS basal medium, calluses producing a few somatic embryos gave rise to numerous somatic embryos, which subsequently developed into plantlets. Plantlets were successfully transplanted to potting soil and grown to maturity in a greenhouse.

• Plant Biotechnology Reports
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• 제2권3호
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• pp.207-212
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• 2008

Plantlets of Alocasia amazonica regenerated under a photon flux density (PFD) of 15 or $30{\mu}mol\;m^{-2}s^{-1}$ showed better growth and development than those grown under higher PFDs. While chlorophyll a and chlorophyll b decreased, the number of stomata increased with increasing PFD. Photoperiods also affected plantlet growth and stomatal development. Highest growth was observed for the short photoperiod (8/16 h) and for equinoctial (12/12 h) light and dark periods. Very few stomata developed in the leaves of plantlets grown under a short photoperiod (8/16 h) and the number of stomata increased with increasing light period. In conclusion, both light intensity and photoperiod independently affect growth of A. amazonica and development of stomata, depending on the intensity and duration of light treatment.

• 한국약용작물학회지
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• 제15권5호
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• pp.315-318
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• 2007

An effective rapid propagation method was established through in vitro cultures of the medicinal plant, Cudrania tricuspidata. In vitro plantlets were obtained from in vitro germinated seeds. The various levels of cytokinins (BAP, Kinetin and TDZ) were tested on multiple shoot formation from plantlets. BAP (1.0 mg/l) treatment induced highest number of multiple shoots. Single shoot cultures gave higher initial shoot numbers than 5 shoots per culture. Among the various culture media, the shoot elongation was optimal on 2 MS basal medium without growth regulators. The IAA (2.0 mg/l) treatment induced highest number of roots. IBA (2.0 mg/l) treatment more promoted in vitro root growth than other concentrations. Rooted shoots were transferred directly to small pots with an artificial soil and successfully acclimatized.

• Journal of Plant Biotechnology
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• 제6권2호
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• pp.87-90
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• 2004

An efficient plant regeneration system in alfalfa (Medicago sativa L.) through somatic embryogenesis was established. Embryogenic callus was obtained by culture of hypocotyl segments on MS medium with 0.02mg $L^{-1}$ IAA and 1.0mg $L^{-1}$ zeatin after 45 days of culture. Embryogenic calli were converted to the somatic embryos when transferred to either MS medium without plant growth regulators (PGRs) or MS medium containing various cytokinin (BA, kinetin and zeatin). Most of the somatic embryos were developed into plantlets on MS medium supplemented with 0.1 mg $L^{-1}$ kinetin. Also, secondary embryos appeared on the surface of primary embryo but they showed abnormal growth. Regenerated plantlets were transplanted to pots containing vermiculite and perlite for further analysis.

• Journal of Plant Biotechnology
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• 제6권3호
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• pp.181-188
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• 2004

Zingiber officinale buds from the rhizomes were used to produce in vitro shoots. These explants produced the largest number of multiple shoots, 9.8 shoots per explant, when were cultured on MS (Murashige and Skoog 1962) medium supplemented with 2.0 mg/L benzyladenine (BA) and 2.0 mg/L indole butyric acid (IBA). This medium was also found to be suitable for in vitro propagation of other Zingiberaceae species: Alpinia conchigera, Alpinia galanga, Curcuma domestica, C. zedoaria and Kaempferia galanga. Both C. domestica and C. zedoaria produced more multiple shoots when were cultured in the liquid proliferation medium, MS medium containing 2.0 mg/L BA and 2.0 mg/L IBA. To maintain the in vitro plantlets of Zingiberaceae species, they were required to subculture every four weeks. After executing proper acclimatization protocol, in vitro plantlets of Alpinia galanga, A. conchigera, Curcuma domestica, C. zedoaria, Kaempferia galanga and Zingiber officinale could be successfully planted in the field with high percentage of survival.

• 한국약용작물학회지
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• 제11권4호
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• pp.316-320
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• 2003

A protocol is described for rapid multiplication of Zanthoxylum piperitum DC. (Rutaceae), an important aromatic and medicinal plant, through shoot-tip explant cultures. Murashige and Skoog (MS) medium supplemented with various concentrations of N-6-benzyladenine (BA), N-6-benzylaminopurine (BAP) and thidiazuron (TDZ), in single or in combination with ${\alpha}-naphthaleneacetic$ acid (NAA), was used to determine the rate of shoot proliferation. N-6-benzyladenine (BA) used at 0.5mg/l, was the most effective in initiating multiple shoot proliferation at the rate of 23 microshoots per shoot-tip explants after 40 days of culture. Shoot multiplication increased 1.2-fold in each successive subculture. Induction of rooting (98%) was achieved by transferring the shoots to the same basal medium containing 2 mg/l indole-3-butyric acid (IBA). Plantlets went through a hardening phase in a controlled growth chamber, prior to in vivo transfer. These results represented that possible application for the mass production of plantlets through in vitro culture system of Zanthoxylum piperitum DC.