• Journal of Microbiology and Biotechnology
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• 제31권9호
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• pp.1288-1294
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• 2021

There are a growing number of reports of hospital-acquired infections caused by pathogenic bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA). Many plant products are now being used as a natural means of exploring antimicrobial agents against different types of human pathogenic bacteria. In this research, we sought to isolate and identify an active molecule from Sedum takesimense that has possible antibacterial activity against various clinical isolates of MRSA. NMR analysis revealed that the structure of the HPLC-purified compound was 1,2,4,6-tetra-O-galloyl-glucose. The minimum inhibitory concentration (MIC) of different extract fractions against numerous pathogenic bacteria was determined, and the actively purified compound has potent antibacterial activity against multidrug-resistant pathogenic bacteria, i.e., MRSA and its clinical isolates. In addition, the combination of the active compound and β-lactam antibiotics (e.g., oxacillin) demonstrated synergistic action against MRSA, with a fractional inhibitory concentration (FIC) index of 0.281. The current research revealed an alternative approach to combating pathogenesis caused by multi-drug resistant bacteria using plant materials. Furthermore, using a combination approach in which the active plant-derived compound is combined with antibiotics has proved to be a successful way of destroying pathogens synergistically.

• Genomics & Informatics
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• 제19권4호
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• pp.46.1-46.11
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• 2021

Moringa oleifera is nowadays raising as the most preferred medicinal plant, as every part of the moringa plant has potential bioactive compounds which can be used as herbal medicines. Some bioactive compounds of M. oleifera possess potential anti-cancer properties which interact with the apoptosis protein p53 in cancer cell lines of oral squamous cell carcinoma. This research work focuses on the interaction among the selected bioactive compounds derived from M. oleifera with targeted apoptosis protein p53 from the apoptosis pathway to check whether the bioactive compound will induce apoptosis after the mutation in p53. To check the toxicity and drug-likeness of the selected bioactive compound derived from M. oleifera based on Lipinski's Rule of Five. Detailed analysis of the 3D structure of apoptosis protein p53. To analyze protein's active site by CASTp 3.0 server. Molecular docking and binding affinity were analyzed between protein p53 with selected bioactive compounds in order to find the most potential inhibitor against the target. This study shows the docking between the potential bioactive compounds with targeted apoptosis protein p53. Quercetin was the most potential bioactive compound whereas kaempferol shows poor affinity towards the targeted p53 protein in the apoptosis pathway. Thus, the objective of this research can provide an insight prediction towards M. oleifera derived bioactive compounds and target apoptosis protein p53 in the structural analysis for compound isolation and in-vivo experiments on the cancer cell line.

• 한국토양비료학회지
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• 제10권2호
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• pp.85-92
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• 1977

인산감수성(燐酸感受性)이 다른 9개대두재배종(個大豆栽培種) Lee, Hill, Harosoy, Clark-63, Chippewa 및 R56-49을 서로 다른 질소원(窒素源)으로 수경재배(水耕栽培)하여 엽중유리당(葉中遊離糖)과 유기산(有機酸)을 gas chronatograph로 조사(調査)하여 미동정(未同定)의 X, Y peak와 Sucrose peak가 주(主) peak로 나타났다. X 화합물(化合物)은 NO3-N 급여(給與)경우 흔적으로 나타난데 반하여 NH4-N나 urea 급여(給與)경우에 다량(多量)나타났다. Y 화합물(化合物)을 Urea 급여(給與)경우 감소하는 경향이었다. Sucrose는 NH4-N 급여(給與)에서 흔적으로 나타나고 Urea에서 NO3-N에서보다 높았다. X 화합물(化合物)은 erythrose에서 유래(由來)한 4 탄소(炭素) 산성당(酸性糖)이거나 Purine이나 Pyrimidine에서 유래(由來)한 환상화합물(環狀化合物)로 Y 화합물(化合物)은 glycolysis path에서 유래(由來)한 6 탄당(炭糖)으로 추정(推定)되었다. Y/X 치(値)는 인산감수성(燐酸感受性)의 좋은 지표(指標)가 되므로(역관계(逆關係)) 이 두 화합물(化合物)은 ammonia 독작용(毒作用) 및 인산감수성(燐酸感受性) 기작(機作)에 관여(關與)하는 요인화합물(要因化合物)로 보였다.

• 생약학회지
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• 제31권4호
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• pp.449-454
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• 2000

In search for plant-derived cytotoxic compounds, it was found that the $CHCl_3$ and EtOAC extracts obtained from the flowers of Paulownia coreana Uyeki (Scrophulariaceae) exhibited significant cytotoxic activity against human tumor cell lines, A549, SK-OV-3, SK-MEL-2, XF498, and HCT15. Activity-guided fractionation on the basis of the inhibitory activity against the growth of human tumor cell lines, in vitro, and repeated column chromatography afforded several cytotoxic compounds from P. coreana. The structures and stereochemistry of these compounds were established, on the basis of analysis of spectra including IR, UV, EI-MS, $^{1}H-NMR,\;^{13}C-NMR$ and some chemical transformations, as Compound PCCl $(2-hydroxy-4(15),11(13)-eudesmadien-8{\beta},12-olide)$, Compound $PCC2(2,3-dihydro-4-hydroxy-1(15),11(13)-xanthadien-8{\beta},12-olide)$, Compound PCE1 (chrysophanol), Compound PCE2 (emodin), Compound PCE3 (physcion). Cytotoxic activity of compounds obtained from P. coreana. on five tumor cells lines was evaluated by procedure of SRB methods.

• 식물병연구
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• 제30권2호
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• pp.189-193
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• 2024

오이(Cucumis sativus L.)은 국내에서 경제적으로 중요한 작물이며, 지속 가능한 생산을 위해서는 다양한 식물병을 효과적으로 관리해야 한다. 본 연구에서는 이전의 연구에서 선발한 오이 탄저병 억제 Streptomyces sp. S20-465가 생성하는 대사산물을 동정하고, Colletotrichum orbiculare에 의한 오이 탄저병 방제에 대한 효과를 규명하였다. Streptomyces sp. S20-465는 YEME 배지에서 효과적으로 생장, 포자 형성 및 항진균 활성이 있었으며, 배양 여과액의 n-hexane 추출물에 함유된 4-hydroxy-4-methyl-2-pentanone이 오이 탄저병을 효과적으로 억제하였다. 본 연구 결과는 Streptomyces sp. S20-465 유래 대사산물이 오이 탄저병 등 식물병 관리에 효과적인 소재로 활용될 수 있음을 제시하며, 이는 오이 생산을 지속 가능하게 향상시키는 방안이 될 수 있을 것이다.

• Biomolecules & Therapeutics
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• 제32권1호
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• pp.38-55
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• 2024

Cancer is a global health challenge with high morbidity and mortality rates. However, conventional cancer treatment methods often have severe side effects and limited success rates. In the last decade, extensive research has been conducted to develop safe, and efficient alternative treatments that do not have the limitations of existing anticancer medicines. Plant-derived compounds have shown promise in cancer treatment for their anti-carcinogenic and anti-proliferative properties. Rosmarinic acid (RA) and carnosic acid (CA) are potent polyphenolic compounds found in rosemary (Rosmarinus officinalis) extract. They have been extensively studied for their biological properties, which include anti-diabetic, anti-inflammatory, antioxidant, and anticancer activities. In addition, RA and CA have demonstrated effective anti-proliferative properties against various cancers, making them promising targets for extensive research to develop candidate or leading compounds for cancer treatment. This review discusses and summarizes the anti-tumor effect of RA and CA against various cancers and highlights the involved biochemical and mechanistic pathways.

• 한국식품영양학회지
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• 제36권6호
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• pp.572-580
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• 2023

Hypertension is characterized by excessive renin-angiotensin system activity, leading to blood vessel constriction. Several synthetic compounds have been developed to inhibit renin and angiotensin-converting enzyme (ACE). These drugs often have adverse side effects, driving the exploration of plant protein-derived peptides as alternative or supplementary treatments. This study assessed the phenolic compound and amino acid content and the antioxidant and antihypertensive activity of 5 South Korean staple crops. Sorghum had the highest phenolic compound content and exhibited the highest antioxidant activity. Millet grains, particularly finger millet (38.86%), showed higher antihypertensive activity than red beans (14.42%) and sorghum (17.16%). Finger millet was found to contain a large proportion of branched-chain, aromatic, and sulfur-containing amino acids, which are associated with ACE inhibition. In particular, cysteine content was positively correlated with ACE inhibition in the crops tested (r=0.696, p<0.01). This study confirmed that the amino acid composition was more correlated with the antihypertensive activity of grains than the phenolic compound content. Finger millet mainly contained amino acids, which have higher ACE inhibitory activity, resulting in the strongest antihypertensive activity. These findings underscore the antihypertensive potential of select crops as plant-based food ingredients, offering insight into their biological functions.

• Journal of Applied Biological Chemistry
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• 제64권2호
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• pp.153-158
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• 2021

Cordyceps militaris (CM) is one of the most important medicinal mushrooms known to possess various biological activities. Cordycepin (CP) is a bioactive compound present in the fruiting bodies of CM and is known to have anti-tumor, anti-metastatic immunomodulatory and anti-inflammatory activities. In this study, we aim to analyze CP quantitatively under various CM extraction conditions. CP was measured using high-performance liquid chromatography, quantified using a reversed phase column using a gradient elution system of water and acetonitrile, and detected with a UV absorbance wavelength of 260 nm. The CP content of CM was the highest in 100% ethanol extract of the fruiting bodies and 60% ethanol extract of the mycelium. This study provides an efficient analysis method to determine the optimal extraction conditions for CP that can be used as a basis for developing functional foods and pharmaceutical products derived from CM.

• Journal of Microbiology and Biotechnology
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• 제25권9호
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• pp.1429-1432
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• 2015

To identify plant-derived cell signaling inhibitors with antifungal properties, a twocomponent screening system using both wild-type Cryptococcus neoformans and a calcineurin mutant was employed owing to their counter-regulatory actions on the Hog1 mitogenactivated protein kinase and calcineurin pathways. Of the 2,000 plant extracts evaluated, a single bioactive compound from M. obovata Thunb. was found to act specifically on the calcineurin pathway of C. neoformans. This compound was identified as magnoloside A, and had potent antifungal activities against various Cryptococcus strains with minimum inhibitory concentration values ranging from 1.0 to 4.0 μg/ml.

• 한국응용곤충학회지
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• 제60권3호
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• pp.315-322
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• 2021

충북 청주지역에서 채집된 작은소피참진드기(Haemaphysalis longicornis)에 대하여 다양한 식물체 메탄올 조추출물과 천궁유래 물질들을 이용하여 살비활성과 기피활성을 조사하였다. 총 24종의 식물추출물을 이용한 작은소피참진드기에 대한 살비활성을 조사한 결과, 천궁(Cnidium officinale)에서 93.3%의 살비율로 가장 높은 활성을 보였으며, 기피 효과의 경우 도라지, 미국자리공, 백목련, 어성초, 천궁, 칡잎 등에서 통계적으로 유의미한 차이를 보였다. 기피 및 살비활성이 가장 높은 천궁의 메탄올추출물을 용매 분획한 물질들과 천궁 유래물질인 butylidenephthalide의 활성을 비교한 결과, 헥산(hexane)층 처리 3일 후에 90%의 높은 살비효과를 보였으며 기피반응도 통계적으로 유의미한 차이를 보였다. 천궁유래물질인 butylidenephthalide은 처리 7일 후에 90%의 살비활성을 보였고 T-tube olfactometer 실험에서 모든 작은소피참진드기가 무처리구로 이동하여 기피활성이 있음을 알 수 있었다. 따라서 본 연구결과는 천궁 조추출물과 천궁유래물질인 butylidenephthalide를 이용하여 작은 소피참진드기의 방제 개발에 기초자료로 활용될 수 있을 것으로 생각된다.