• 생물환경조절학회지
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• 제6권4호
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• pp.264-269
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• 1997

본 실험은 고기능성 채소 생산을 위한 배양액내의 적정 셀레니움 농도를 구명하고자 수행되었다. 벨기에의 채소연구소에서 허브재배를 위해 개발된 양액을 이용하여 각각 $Na_2$SeO$_4$를 0, 2, 4, 6, 8mg/$\ell$농도로 처리하였다. 배양액내 셀레니움의 농도가 국화과 식물인 향쑥의 생육에 미치는 영향을 알아본 결과 저농도의 처리는 생육을 향상시켰으나 6mg/$\ell$이상의 고농도 처리는 생육을 감소시켰다. 엽록소의 함량은 셀레니움 처리에 의해 증가되었는데 배양액 내의 selenate ion 농도가 높을수록 전체 엽록소함량도 증가되었다. 비타민 C의 함량은 좋은 생장을 보였던 4mg/$\ell$처리까지는 증가하였으나 그 이상으로 농도가 증가했을 때 비타민C의 함량은 감소하였다. 저농도의 selenate 이온 농도는 정유의 함량을 증가시켰으나 고농도에서는 정유의 함량이 감소되었다. 식물에 의한 셀레니움의 흡수는 배양액내의 selenate 이온 농도가 증가할수록 촉진되었다.

• Journal of Microbiology and Biotechnology
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• 제18권2호
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• pp.299-307
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• 2008

Soybean seed ferritin is essential for human iron supplementation and iron deficiency anemia prevention because it contains abundant bioavailable iron and is frequently consumed in the human diet. However, it is poorly understood in regards its several properties, such as iron mineralization, subunit assembly, and protein folding. To address these issues, we decided to prepare the soybean seed ferritin complex via a recombinant DNA approach. In this paper, we report a rapid and simple Escherichia coli expression system to produce the soybean seed ferritin complex. In this system, two subunits of soybean seed ferritin, H-2 and H-1, were encoded in a single plasmid, and optimal expression was achieved by additionally coexpressing a team of molecular chaperones, trigger factor and GroEL-GroES. The His-tagged ferritin complex was purified by $Ni^{2+}$ affinity chromatography, and an intact ferritin complex was obtained following His-tagged enterokinase (His-EK) digestion. The purified ferritin complex synthesized in E. coli demonstrated some reported features of its native counterpart from soybean seed, including an apparent molecular weight, multimeric assembly, and iron uptake activity. We believe that the strategy described in this paper may be of general utility in producing other recombinant plant ferritins built up from two types of subunits.

• 한국토양비료학회지
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• 제34권3호
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• pp.213-236
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• 2001

토양용액의 이온조성은 작물의 양분흡수와 밀접한 관계를 가지고 있으며 양분의 이온조성을 추정하기 위하여 많은 모델이 개발되어 사용되고 있다. 토양용액 중 분석되는 양분의 몰농도는 insoluble 이온쌍과 평형을 이루고 있는 soluble 이온과 이온쌍 그리고 complex등의 농도로 간주하고, 양이온과 음이온을 동시에 고려한 양분의 이온조성을 추정하기 위하여 분석된 양분의 몰농도에 따른 선정된 평형식의 평형상수에 대한 conditional equilibrium constant를 구하여 모든 평형식이 만족되는 이온과 이온쌍의 몰농도를 구하는 모델을 설정하였다. 본 모델 이용시 기본적으로 필요한 성분은 pH, Eh, EC, 양이온 8종(K, Ca, Mg, Na, Fe, Mn, Al, $NH_4{^+}$, 음이온 6종(Si, 5, p Cl, $NO_3{^-}$, $HCO_3{^-}$)이며 다른 성분도 추가할 수 있도록 하였다. 토양과 토양용액이 평형조건인 경우 추정된 이온과 이온쌍의 조성과 이에 따른 존재 가능한 침전량과 mineral 종류별 함량을 계산하였다.

• 한국토양비료학회지
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• 제49권4호
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• pp.327-334
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• 2016

In order to compare phosphorus (P) behavior of livestock manure compost (LMC) and chemical fertilizer (CF) applied to the sea-reclaimed land soils, incubation experiments were conducted for five weeks. Four soils differing textural classes, sandy loam and clay loam, and electric conductivity (EC) value, high and low, were applied with CF and LMC. LMC was applied at the level of 0, 1, 2, and 3% on the soil weight basis and CF was applied at the same levels of P as LMC. The results showed that increase of P application rate of CF and LMC led to linearly increase available $P_2O_5$ and $0.01M\;CaCl_2$ extractable P contents regardless of soil texture and EC. However, 0.01M $CaCl_2$ extractable P from soil applied with CF was significantly higher than with LMC. Correlation analysis between $0.01M-CaCl_2$ extractable P and fractionated P by different extraction methods showed that $0.01M-CaCl_2$ extractable P positively correlated with KCl-P (soluble and exchangeable P) and HCl-P (Ca and Mg bound P). However, NaOH-P (Fe and Al bound P and organic P) and residual P was adverse. The amount of NaOH-P significantly influenced to the amount of 0.01M $CaCl_2$ extractable P of CF and LMC in the soils. The application of LMC at sandy loam soil could be carried out in the consideration of nutrient leaching and crop uptake.

• 한국토양비료학회지
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• 제42권Spc호
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• pp.51-56
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• 2009

토양학과 비료를 가르치기 위하여 다양한 방법이 동원되고 있다. 카툰은 강의자와 학생 모두에게 도움이 되는 가장 효과적인 방법의 하나다. 강의자는 강의하고자 하는 장에 근거한 이야기를 전개하는 축이 된다.? 그리고 카툰은 표현하고자 하는 내용을 정확하고 간결하게 표현하기 위한 정상적인 문장을 선택하고난 다음 적정한 삽화를 삽입하여야 한다. 카툰이 다루어야 할 주요 내용은 용어풀이, 영양흡수, 산도, 염류 축척, 수분, 오염, 화학비료, 퇴비, 그리고 일반 토양과 관련한 분야가 포함된다.

• 한국토양비료학회지
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• 제49권6호
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• pp.669-676
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• 2016

A field study was conducted to determine the runoff loss of N and P in small scale of red pepper field plots (10% slope), consisting of three different plots with black polyethylene vinyl mulching (mulching), ridge without mulching (ridge), and flat without ridge and mulching (flat). Composted manure and urea as a basal application were applied at rates of $20MT\;ha^{-1}$ and $93kg\;N\;ha^{-1}$, respectively. Urea at $189kg\;N\;ha^{-1}$ and fused phosphate at $67kg\;P_2O_5\;ha^{-1}$ were additionally applied on June 25 with different fertilization methods, broadcast application in flat plot and hole injection in ridge and mulching plots. Plant uptake of N and P was positively correlated with their respective concentrations in surface soil: mulching > ridge > flat plots. The runoff loss by soil erosion was higher in flat plot than ridge and mulching plot with contour line. Nitrate loss by the runoff water had no significant differences among three surface management practices, but the higher average value in ridge and mulching plots than flat plot. Especially, the flat plot had no phosphate loss during summer season. This is probably due to low labile P content in surface soil of flat plot. In the summation of soil and water loss, flat plot was higher in N and P loss than ridge and mulching plot with contour line. Nevertheless, the nitrate and phosphate loss by runoff water could be more important for non-point source management because the water could meet the river easier than eroded soil because of re-deposition around slope land.

• 한국토양비료학회지
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• 제49권6호
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• pp.712-719
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• 2016

Understanding N mineralization dynamics in soil is essential for efficient nutrient management. An anaerobic incubation experiment was conducted to examine N mineralization potential and N mineralization rate of the organic amendments with different C:N ratio in paddy soil. Inorganic N in the soil sample was measured periodically under three temperature conditions ($20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$) for 90 days. N mineralization was accelerated as the temperature rises by approximately $10%^{\circ}C^{-1}$ in average. Negative correlation ($R^2=0.707$) was observed between soil inorganic N and C:N ratio, while total organic carbon extract ($R^2=0.947$) and microbial biomass C ($R^2=0.824$) in the soil were positively related to C:N ratio. Single exponential model was applied for quantitative evaluation of N mineralization process. Model parameter for N mineralization rate, k, increased in proportion to temperature. N mineralization potential, $N_p$, was very different depending on C:N ratio of organic input. $N_p$ value decreased as C:N ratio increased, ranged from $74.3mg\;kg^{-1}$ in a low C:N ratio (12.0 in hairy vetch) to $15.1mg\;kg^{-1}$ in a high C:N ratio (78.2 in rice straw). This result indicated that the amount of inorganic N available for crop uptake can be predicted by temperature and C:N ratio of organic amendment. Consequently, it is suggested that the amount of organic fertilizer application in paddy soil would be determined based on temperature observations and C:N ratio, which represent the decomposition characteristics of organic amendments.

• 전기전자학회논문지
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• 제19권3호
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• pp.295-303
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• 2015

지구 온난화 및 탄소배출권 저감을 위한 대안으로 에너지저장장치(ESS, Energy Storage System)의 중요성을 인식하고 ESS 산업육성을 위한 보급사업 및 인센티브 정책을 추진하고 있다. 우리나라는 2020년까지 ESS 누적 용량 2GW 보급을 추진하고 있다. 이러한 보급정책과 기업들의 기술개발에 따라 시장 확대로 인한 ESS의 배터리 가격이 점차 하락할 전망이다. 본 논문에서는 ESS 기술개발에 따른 ESS 가격 하락에 따른 ESS 보급량 추정을 하였으며, 이에 따라 발생하는 비용의 경제성 분석을 실시하였다. 또한 ESS 종류에 따라 발생할 수 있는 비용 및 ESS와 풍력전원의 연계에 따른 투자비용 분석을 하였으며, ESS보급에 따라 발생할 수 있는 전력판매수익 및 신재생에너지공급인증서 판매수익에 대한 경제성 분석을 실시하였다.

• 한국작물학회지
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• 제47권3호
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• pp.221-225
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• 2002

본 연구는 벼 휴립건답직파 절수재매시 완효성비료의 효과를 알아보기 위하여 질소비료를 표준시비(N-160kg/ha) 대비 완효성비료(LCU: 18-12-13%)를 기비로 100%와 80% 시용구 및 기비로 60% 시용하고 속효성비료인 요소로 분얼비 또는 수비로 20%씩 각각 시용하여 시험한 결과를 요약하면 다음과 같다. 1. 생육기간중 지상부 생육은 LCU 100%에서 가장 컸고, LCU 시비량간에는 시비량이 많을수록 컸다. 2. 시비질소의 흡수량은 LCU 100%에서 가장 높았고 질소 시비효율은 LCU 60% + 수비(요소) 20% 시용구에서 가장 높았다. 3. 처리간 수량구성요소를 보면 $m^2$당 수수와 $m^2$당 립수는 LCU 100%에서 가장 많았고, 등숙비율과 현미 천립중은 비슷하였는데, 쌀 수량은 관행인 속효성 시비구 대비 LCU 100%에서 7% 증수되었다. 따라서 벼 휴립건담직파재배에서 관개수 부족시 절수재배가 필요하여 이때에 완효성비료로 시비할 경우 표준시비량(N-160kg/ha) 수준으로 시비해야 할 것으로 생각된다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.205-205
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• 2017

An excess soil water condition is one of the major problems for the field crops growing in paddy fields because of their poor drainage and less availability for oxygen uptake which leads to adversely affect the photosynthesis. Therefore, the current study was undertaken with aim to investigate the effects groundwater level on the photosynthetic response of soy bean (Urum), red bean (Arari), sesame (Geonbaek), perilla (Dayu) after the transplanting to the lysimeter to investigate the plant-water relation and their effect on photosynthesis. The chlorophyll content of the crops according to the humid conditions of the soy bean, sesame and the perilla was found to be 5%, 6.89 % and 13.7% higher than that of the groundwater treated at 40cm, respectively. On the other hand, the chlorophyll content of adzuki bean decreased 6.6% from the groundwater level of 40cm, and the sorghum decreased by 5.7%. As a result of investigating the Fv / Fm value of groundwater, the adzuki bean at 20cm above groundwater was lower than that of groundwater by 40cm immediately before flowering. The Fv / Fm value of soy bean and sesame at 40cm above groundwater were lowered by flowering under groundwater 20 cm and Fv / Fm value of sorghum is increased at 40 cm treatment immediately before flowering while the Fv / Fm values of the perilla had no significant difference in comparison to those at 20 cm and 40 cm of groundwater. In the case of chlorophyll fluorescence reaction, it is known that the when the absolute value is closer to 0.82, the stress is considered less. As a result of comparing the numerical values of the crops, it was found that the sorghum was the most stressed followed by adzuki bean and sesame, while the soy beans and perilla was found on the average, as they received less stress.