• Title/Summary/Keyword: Plant uptake

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Speculation on the Identity of Bacteria Named TFOs Occurring in the Inefficient P-Removal Phase of a Biological Phosphorus Removal System

  • Lee, Young-Ok;Ahn, Chang-Hoon;Park, Jae-Kwang
    • Environmental Engineering Research
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    • v.15 no.1
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    • pp.3-7
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    • 2010
  • To better understand the ecology of tetrade forming organisms (TFOs) floating in a large amount of dairy wastewater treatment plant (WWTP) effluent (sequencing batch reactor [SBR]) during the inefficient phosphorus (P) removal process of an enhanced biological P removal system, the TFOs from the effluent of a full scale WWTP were separated and attempts made to culture the TFOs in presence/absence of oxygen. The intact TFOs only grew aerobically in the form of unicellular short-rods. Furthermore, to identify the intact TFOs and unicellular short-rods the DNAs of both were extracted, analyzed using their denaturing gradient gel electrophoresis (DGGE)-profiles and then sequenced. The TFOs and unicellular short-rods exhibited the same banding pattern in their DGGE-profiles, and those sequencing data resulted in their identification as Acinetobacter sp. The intact TFOs appeared in clumps and packages of tetrade cells, and were identified as Acinetobacter sp., which are known as strict aerobes and efficient P-removers. The thick layer of extracellular polymeric substance surrounding Acinetobacter sp. may inhibit phosphate uptake, and the cell morphology of TFOs might subsequently be connected with their survival strategy under the anaerobic regime of the SBR system.

Fertilization Efficiency of Livestock Faeces Composts as Compared to Chemical Fertilizers for Paddy Rice Cultivation

  • Kang, C.S.;Roh, A.S.;Kim, S.K.
    • Korean Journal of Organic Agriculture
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    • v.19 no.spc
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    • pp.182-185
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    • 2011
  • Soil $NH_4$-N content became higher in proportion to the increase in the urea application rate, while in livestock faeces compost (LFC) plots, it became lower than in urea plots and had no significant difference statistically among LFC plots. There was a close relationship between phosphate fertilization rate and the increment of soil available phosphate content after experiment resulting y=0.1788x-6.169 ($R^2=0.9425$) when applied fused superphosphate fertilizer, and y=0.0662x-2.689 ($R^2=0.9315$) when applied LFCs by the same amounts of phosphate (x: phosphate application, kg $ha^{-1}$, y: increment in soil available phosphate content, mg $kg^{-1}$. Plant height, number of stems, nutrients uptake by rice and rice yield showed higher levels in N 100, 150% application plots of chemical fertilizers, while every LFC plots exhibited lower values and no significant difference among them. Relative nitrogen fertilization efficiencies of LFCs compared to urea was 12.3% for cattle faeces compost (CaFC), 8.8 for swine faeces compost (SwFC) and 24.6 for chicken faeces compost (ChFC), respectively.

Nutrient and Water Uptake of Tomato and Cucumber Plant by Rockwool and Perlite in medium Culture (암면과 펄라이트 배지에 따른 토마토와 오이의 양분흡수 특성)

  • 김형준;김완순;우영회;조삼증
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 2001.04b
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    • pp.77-78
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    • 2001
  • 순환식 양액재배에서 근권부 배지의 종류가 작물의 양분흡수에 미치는 영향을 구명하고자 하였다. 배지별 양액흡수량은 토마토 및 오이 모두 같은 경향이었는데, 단위일사량당 양액흡수량을 보면 오이는 생육 전반기인 6월 5일까지 지속적으로 양액흡수가 이루어지다가 일사량에 따라 전체 양액의 흡수가 변화하였다. 그러나 단위일사량당 양액흡수량은 생육 후반기에 일정한 수준을 유지하였는데 이것은 노화된 하엽을 제거하였기 때문으로 보인다(Fig 1). 배지에 따른 양액흡수량은 전체적으로 암면에서 생육 후기까지 다소 높은 경향을 보였으나 통계적으로 유의성은 인정되지 않았다. 또한 배지간의 무기이온 흡수율은 이온별로 차이가 있었으나 모든 이온에서 유의성은 인정되지 않았다. 배지에 따른 무기이온 흡수량은 전체적으로 암면에서 생육 후기까지 다소 높은 경향을 보였으나 통계적으로 유의성은 인정되지 않았다(Fig. 2). 작물의 생육 및 수량에서도 암면에서 전반적으로 높은 경향을 보였으나 통계적으로 유의성을 보이지는 않았으나 오이의 엽중은 암면에서 무거웠고 엽건물중도 차이가 있었다. 기타 생육 요소간에는 유의성이 인정되지 않았지만 암면에서 다소 좋은 경향을 보였다(Table 1). 따라서 작물의 무기이온 흡수량을 추정하는 모델식의 입력요소로 배지 종류는 적합하지 않았으며 다만 수분 보유력이 좋은 암면에서 양액 흡수량이 많았다는 결과를 얻었다.

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Investigation into Tritium Behaviour in Chinese cabbage and Rice after a Short-term Exposure of HTO (HTO 피폭후 배추와 벼에서 삼중수소 거동)

  • Kim, Sang-Bog;Lee, Myung-Ho;Choi, Gun-Sik;Choi, Young-Mo;Lee, Chang-Woo
    • Journal of Radiation Protection and Research
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    • v.23 no.2
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    • pp.75-82
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    • 1998
  • Tritium concentration in the atmosphere during HTO exposure was different between two experiments due to different velocity of HTO evaporation. Assimilation rate of the rice plant was considered to be higher than that of Chinese cabbage The uptake of atmospheric HTO into tissue free water tritium(TFWT) was higher in rice plants than in Chinese cabbage. However, organically bound tritium(OBT) concentration is relatively higher in Chinese cabbage than in rice. The specific activity ratio(SAR) increased slowly after HTO exposure and decreased gradually with time. The behaviour of HTO in the soil was affected by the environmental conditions.

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Assessment of Nitrogen Fate in the Soil by Different Application Methods of Digestate (혐기성 소화액의 농지환원에 따른 질소 거동)

  • Nkombo, Laure Lysette Chimi;Hong, Seong Gu
    • Journal of The Korean Society of Agricultural Engineers
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    • v.63 no.3
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    • pp.35-45
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    • 2021
  • Digestate or slurry produced from anaerobic digestion is mostly applied to crop lands for its disposal and recovering nutrients. However, minimizing nitrogen losses following field application of the digestate is important for maximizing the plant's nitrogen uptake and reducing environmental concerns. This study was conducted to assess the effects of three different biogas digestate application techniques (sawdust mixed with digestate (SSD), the hole application method (HA), and digestate injected in the soil (SD)) on nitrate leaching potential in the soil. A pot laboratory experiment was conducted at room temperature of 25 ± 2 ℃ for 107 days. The experimental results showed that sawdust application method turned out to be appropriate for quick immobilization of surplus N in the form of microbial biomass N, reflecting its lower total nitrogen and NH4-N contents and low pH. The NH4-N and total nitrogen fate in the soil fertilized with manure showed no statistically significant (p > 0.05) differences between the different methods applied during the incubation time under room temperature. In contrast, NO3-N concentration indicates significant reduction in sawdust treatment (p < 0.05) compared to the control and other application methods. However, the soil sawdust mixed with digestate was more effective than the other methods, because of the cumulative labile carbon contents of the amendment, which implies soil net N immobilization.

Green tea and type 2 diabetes

  • Park, Jae-Hyung;Bae, Jae-Hoon;Im, Sung-Soon;Song, Dae-Kyu
    • Integrative Medicine Research
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    • v.3 no.1
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    • pp.4-10
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    • 2014
  • Green tea and coffee consumption have been widely popular worldwide. These beverages contain caffeine to activate the central nervous system by adenosine receptor blockade, and due to the caffeine, addiction or tolerance may occur. In addition to this caffeine effect, green tea and coffee consumption have always been at the center of discussions about human health, disease, and longevity. In particular, green tea catechins are involved in many biological activities such as antioxidation and modulation of various cellular lipid and proteins. Thus, they are beneficial against degenerative diseases, including obesity, cancer, cardiovascular diseases, and various inflammatory diseases. Some reports also suggest that daily consumption of tea catechins may help in controlling type 2 diabetes. However, other studies have reported that chronic consumption of green tea may result in hepatic failure, neuronal damage, and exacerbation of diabetes, suggesting that interindividual variations in the green tea effect are large. This review will focus on the effect of green tea catechins extracted from the Camellia sinensis plant on type 2 diabetes and obesity, and the possible mechanistic explanation for the experimental results mainly from our laboratory. It is hoped that green tea can be consumed in a suitable manner as a supplement to prevent the development of type 2 diabetes and obesity.

Anti-Inflammatory Effects of Hydroethanolic Extract from Ehretia asperula on Lipopolysaccharide-Stimulated RAW264.7 Macrophages

  • Bao Le;Vo Thi Kim Hong;Seung Hwan Yang
    • Journal of Microbiology and Biotechnology
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    • v.34 no.6
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    • pp.1340-1347
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    • 2024
  • Ehretia asperula is a medicinal plant of the Ehretiaceae family used to treat inflammatory disorders, but the underlying mechanisms are not fully elucidated. The anti-inflammatory potential was determined based on enzyme cyclooxygenase-2 (COX-2) inhibition, which showed that the 95% ethanol extract (95ECH) was most effective with a half-maximal inhibitory concentration (IC50) value of 34.09 ㎍/mL. The effects of 95ECH on phagocytosis, NO production, gene, and protein expression of the cyclooxygenase 2/prostaglandin E2 (COX-2/PGE2) and inducible nitric oxide synthase/ nitric oxide (iNOS/NO) pathways in lipopolysaccharide (LPS)-induced RAW264.7 cells were examined using the neutral red uptake and Griess assays, reverse-transcriptase polymerase chain reactions (RT-PCR), and enzyme-linked immunosorbent assays (ELISA). The results showed that 95ECH suppressed phagocytosis and the NO production in activated macrophage cells (p < 0.01). Conversely, 95ECH regulated the expression levels of mRNAs for cytokines tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) as well as the corresponding proteins. In addition, PGE2 production was inhibited in a dose-dependent manner by 95ECH, and the expression of iNOS and COX-2 mRNAs was decreased in activated macrophage cells, as expected. Therefore, 95ECH from E. asperula leaves contains potentially valuable compounds for use in inflammation management.

Concepts concerning various nitrogen efficiencies and their interrelation in rice plant (수도(水稻)에서 여러 질소효율의 개념(槪念)과 상호관계(相互關係))

  • Park, Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.8 no.2
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    • pp.69-80
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    • 1975
  • Relationships between yield and various nitrogen efficiencies, between efficiencies and between efficiency and nitrogen uptake amount of rice plant were proposed and tested using data from N.P.K simple trials about 30 to 50 locations, for three years. Established relationships are well in accordance with experimental results by showing highly significant correlations between them. The overall indications are that high yielding capacity of fields with fertilizer application, depends primarily on high fertilizer nitrogen uptake by increasing fertilizer use efficiency (Eu), secondly the efficiency (Ef) of absorbed fertilizer nitrogen (Nf) and fertilization efficiency (Fe) and also depends much on nitrogen efficiency for grain yield (E) to great extend and that the efficiency (Es) of soil nitrogen (Ns) contributes to E more than Ef does. All nitrogen efficiencies are negatively correlated with the uptake amount of corresponding nitrogen and counterpart efficiency. Es and Ef could be determined firstly by difference method and secondly E versus Cs (Cs=Ns/Ns+Nf) plotting and thirdly E-Cs plotting with labelled fertilizermethod using the equation E=Es Cs+B where B=Ef Cf but a constant under the given condition and at last Y-Ns plotting with labelled fertilizer using Eq Y=$Es{\cdot}Ns+B$ where B=$Ef{\cdot}Nf$. Es which seems not much variable from field to field is mostly greater (about 80% of tested fields) than Ef which is much variable and depends much on fertilizer form. The relationships tested and well agreed are as follows: 1. Y=$Es{\cdot}Ns+Ef{\cdot}Nf$ (Y is yield) 2. E=$Es{\cdot}Cs+Ef{\cdot}Cf$ where Cf=Nf/Nf+Ns 3. E=b-aN where E=E, Es or Ef and N=N, Ns or Nf respectively, (E=Y/N, N=Nf+Ns), b is theoretical maximum under the given system and a is tangent at N=O of the curve, Y=EN. 4. Fe=Ef Eu and Se=$Es{\cdot}Eu$ where Se is efficiency of soil available nitrogen. 5. E=$(Se{\cdot}Cs+Fe{\cdot}Cf)/Eu$ 6. Y=$Es{\cdot}Eu{\cdot}Sf+Ef{\cdot}Eu{\cdot}Fn$or Y=$Es{\cdot}Eu{\cdot}Ea{\cdot}Sn+Ef{\cdot}Eu{\cdot}Fn $where Sf=$Ea{\cdot}Sn$, Ea is soil available nitrogen equivalent to fertilizer(Sf) divided by total soil nitrogen (Sn).

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Effect of Salt Concentration in Soil on the Growth, Yield, Photosynthetic Rate, and Mineral Uptake of Tomato in Protected Cultivation (토양 염류농도가 시설토마토의 생육, 수량, 광합성속도 및 무기양분 흡수에 미치는 영향)

  • Rhee, Han-Cheol;Cho, Myeung-Whan;Lee, Si-Young;Choi, Gyeong-Lee;Lee, Jae-Han
    • Journal of Bio-Environment Control
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    • v.16 no.4
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    • pp.328-332
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    • 2007
  • This study was conducted to investigate the effects of high concentrations of salts in soil on the growth, yield, quality, photosynthetic rate, and mineral uptake of tomato ('House Momotaro') in pot cultivation. The growth of tomato such as plant height, top plant weight and root weight decreased as the concentrations of salts in soils increased. Yield decreased by 31% and 41% in EC 5.0 and $7.5dS{\cdot}m^{-1}$, respectively compared with the salt concentration of EC $1.5dS{\cdot}m^{-1}$. Yield reduction was caused by low mean weight and number of fruit if at high salt concentration in soil, and affected by low photosynthetic rate and water potential in leaf, The rate of blossom-end rot was highest (16.7%) in EC $7.5dS{\cdot}m^{-1}$ and increased as the concentrations of salts in soils increased. The contents of soluble solids and titratable acids showed a tendency to increase with increasing the concentrations of salts in soils. Photosynthetic rate, water potential and stomatal conductance in leaf decreased as the salt concentration in soil increased. The higher the salt concentration in soil, the lower the mineral uptake such as T-N, P, K, Ca and Mg but, the higher the content of Na.

Changes of Nitrogen Uptake, Growth and Activities of Nitrogen Metabolizing Enzymes by Different Source of Nitrogen in Tobacco (담배에서 질소 형태에 따른 흡수 양상 및 생육과 질소대사 효소의 활성 변화)

  • 이상각;심상인;강병화;배길관
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.5
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    • pp.515-521
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    • 1997
  • Tobacco plant was grown for 40 days hydroponically in nutrient solutions composed of different forms of nitrogen, like NO$_3$$^{[-10]}$ -N, NH$_4$$^{+}$-N, and a mixed formulation of NO$_3$$^{[-10]}$ -N and NH$_4$$^{+}$-N. Uptake response, nitrate reductase, and glutamine synthetase activity at growth stage were investigated to understand the basic knowledge of nitrogen metabolism. The better growth of shoot and root was observed in the mixed nutrient solution than NO$_3$$^{[-10]}$ -N or NH$_4$$^{+}$-N, alone. The plant growth in NH$_4$$^{+}$-N nutrient solution was poor due to ammonium toxicity. The pH of nutrient solution containing NO$_3$$^{[-10]}$ -N increased up to 40 days after transplanting. But the pH of solution containing NO$_3$$^{[-10]}$ -N decreased drastically to 3.42 at 20 days after transplant. The pH in the mixed formulation dropped to pH 3.64 at 30 days after transplant and showed re-increase. It is assumed that nitrogen of NH$_4$$^{+}$-N form was taken up preferentially at early stage and NO$_3$$^{[-10]}$ -N form was taken up preferentially at middle stage in the treatment with the mixed solution. The result indicates that the relative proportion of nitrogen forms affected the uptake patterns at each growth stages. The contents of chlorophyll and soluble protein were high with the mixed solution. Total nitrogen content was the highest in NH$_4$$^{+}$-N solution and the content also increased by the application of the mixed type of nitrogen. The amount of nitrate in leaves was high in NO$_3$$^{[-10]}$ -N treatment and the amount of ammonium was high in NH$_4$$^{+}$-N treatment. The activity of nitrate reductase or glutamine synthetase was highest in the leaves grown in mixed nutrient solution than in those with any other single of nitrogen form.

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