• 제목/요약/키워드: Plant molecular biology

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ORGANIZATION AND EXPRESSION OF CALMODULIN GENES IN PLANTS

  • Sang Hyoung Lee;Jong Cheol Kim;Mal Soon Lee;Young Ju Choi;Jong Chan Hong;Jeong Dong Bahk
    • 한국식물학회:학술대회논문집
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    • 한국식물학회 1993년도 제7회 식물생명공학 심포지움 식물 세포 분화의 분자적 접근 Seventh Symposium on Plant Biotechnology -Approach to Plant Cell Differentiation-
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    • pp.71-88
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    • 1993

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Large-scale identification of lettuce and cucumber phloem proteins

  • Chen, Xiong-Yan;Kim, Su-Hwa;Shi, Chun-Lin;Rim, Yeong-Gil;Moon, Ju-Yeon;Jung, Jin-Hee;Kwon, Hey-Jin;Kim, Sun-Seon;Kwon, Seo-Nok;Park, Zee-Yong;Kim, Jae-Yean
    • 한국식물생명공학회:학술대회논문집
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    • 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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    • pp.341-341
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    • 2005

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Molecular Analysis of Rice Plants Harboring an Ac/Ds Transposable Element-Mediated Gene Trapping System

  • Hang Gyeong;Mi Sook Choe;Sung-Ho Lee;Sung Han Park;Hyuk Kim;Ja Choon Koo;No Youl Kim;Su Hyun Park;Jeung Joo Lee
    • 한국식물학회:학술대회논문집
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    • 한국식물학회 1999년도 제13회 식물생명공학심포지움 New Approaches to Understand Gene Function in Plants and Application to Plant Biotechnology
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    • pp.17-20
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    • 1999

  • In rice, limited efforts have been made to identify genes by the use of insertional mutagens, especially heterologous transposons such as the maize Ac/Ds. We constructed Ac and gene trap Ds vectors and introduced them into the rice genome by Agrobacterium-mediated transformation. In this report, rice plants that contained single and simple insertions of T-DNA were analyzed in order to evaluate the gene-tagging efficiency. The 3'end of Ds was examined for putative splicing donor sites. As observed in maize, three splice donor sites were identified at the 3'end of the Ds in rice. Nearly 80% of Ds elements wered excised from the original T-DNA sites, when Ac cDNA was expressed under a CaMV 35S promoter. Repetitive ratoon culturing was performed to induce new transpositions of Ds in new plants derived from cuttings. About 30% of the plants carried at least one Ds that underwent secondary transposition in the later cultures. 8% of transposed Ds elements expressed GUS in various tissues of rice panicles. With cloned DNA adjacent to Ds, the genomic complexities of the insertion sites were examined by Southern hybridization. Half of the Ds insertion sites showed simple hybriodization patterns which could be easily utilized to locate the Ds. Our data demonstrate that the Ac/Ds mediated gene trap system could prove an excellent tool for the analysis of functions of genes in rice. We discuss genetic strategies that could be employed in a largee scale mutagenesis using a heterologous Ac/Ds family in rice.

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