• Title/Summary/Keyword: Plant injury

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Development of a Specific antibody for the Detection of Ice Nucleation-Active Bacteria (빙핵세균의 검출을 위한 특이적 항혈청 개발)

  • Lee, Ung;Kwon, Mi-Kyung;Seong, Ki-Young;Cho, Baik-Ho;Kim, Ki-Chung
    • Plant Disease and Agriculture
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    • v.5 no.1
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    • pp.27-33
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    • 1999
  • Frost injury of crops is closely related to the epiphytic population dynamics of ice nucleation-active (INA) bacteria, and the injury can be reduced by decreasing the INA bacterial population. In order to predict the epiphytic population of INA bacteria on crops, a rapid and accurate detection method has to be developed. In the previous report, we produced some antibodies against INA proteins purified from the outer membrane of INA bacteria. However it was difficult to produce the antibodies because the purification procedures of the INA proteins were complicated, and the final yield was too low. We designed a specific peptide from the N-terminal region of INA protein by computer analysis and synthesized the peptide in vitro in this experiment. The peptide sequence was Asp-Ser-Por-Leu-Ser-Leu-His-Ala-Asp, that is corresponding to the highly conserved region in several INA proteins, with predicted beta turn, coiling, and hydrophilic region. A polyclonal anti-INA peptide antiserum produced specifically recognized INA bacteria as few as 10 colony-forming units (CFU) in the ELISA reactions and did not respond to other non-INA bacteria. Serological specificity of the anti-INA peptide antiserum will facilitate the forecasting of the INA bacterial population dynamics on crops.

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Screening of Seed Disinfectant for Controlling Brown Leaf Blight in Alisma plantago Double Cropping after Early Rice

  • Shin, Dong-Young;Kwon, Byung-Sun;Lim, June-Taeg;Hyun, Kyu-Hwan;Lim, Jung-Mook;Seo, Young-Nam
    • Korean Journal of Plant Resources
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    • v.19 no.6
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    • pp.692-696
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    • 2006
  • This study was conducted to evaluate the effect of seed disinfectant, in control of brown leaf blight, growth characteristics, and dry root yield in the cultivation of Alisma plantago after early maturing rice cropping. Experimental plot was laid out in split plots design with three replications. The major seed disinfectants were benomyl Wp, 20%, Captan Wp, 50%, Triferine Ec, 17%, Etridia zole Ec, 25%, and Thioplant-mythyl Wp, 50%. Even though seed disinfectant treated had no effect on the growth and flowering date of Alisma plantago, dry root yield was increased largely with benomyl Wp, 20%, in seed disinfectant than in the other seed disinfectants and contorl. All seed disinfectants had no injury with standard dosage. But all seed disinfectants had slight injury in the double dosage level for the Alisma plantago. On the basis of yield, vegetative and disease paramerer, benomyl Wp (20%) ($100g/20{\ell}$) had shown superior performance, however, all the seed disinfectants are effective as compare to without treatment.

Chemical Control of Sclerotinia rot in Rapeseed Double Cropping after Rice

  • Sun Kwon-Byung;Lim June-Taeg;Jung Dong-Soo;Shin Jong-Sup
    • Korean Journal of Plant Resources
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    • v.19 no.3
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    • pp.392-396
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    • 2006
  • This study was conducted to evaluate the control effect of fungicides of sclerotinia rot control, growth characteristics, and seed yield in the cultivation of rapeseed. All fungicides treated had no effect on the growth of rapeseed. The major fungicides were mancozeb 75% WP, chlorothalonil 75% WP, dithianon 43% WP. difenoconazole 10% WP, benomyl 50% WP, and propineb 70% WP. Dry seed yield and control were increased largely with chlorothalonil 75% WP ($33g/12{\ell}$), fungicide than the other fungicides Sclerotinia rot. All fungicides had no injury with standard dosage. On the other hand all fungicides had slight injury in the double dosage level for the rapeseed.

Chemical Control of Gray Mold in Scutellaria baicalensis Georg

  • Kwon, Byung-Sun;Shin, Dong-Young
    • Plant Resources
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    • v.8 no.2
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    • pp.130-134
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    • 2005
  • This study was conducted to evaluate the control effect of fungicides on control of Gray mold, growth characteristics, and root yield in the cultivation of Scutellaria baicalensis after barley cropping. All fungicides treated had no effect on the growth and flowering rate of Scutellaria baicalensis. All seed disinfectants had no effect on the growth and flowering date of Scutellaria baicalensis Georg. The major seed disinfectants were Benomyl Wp, $20\%$, Captan Wp, $50\%$, Hymexazol Ec, $30\%$, Carboxin D, $37.5\%$. Dry root yield were increased largely with Benomyl Wp, $20\%$, seed disinfectant than the other seed disinfectants had no injury with standard dosage. On the other hand, all seed disinfectants had slight injury in the double dosage level for the Scutellaria baicalensis Georg.

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Field Survey of Moisture Injury in Peucedanum japonicum Thunberct and Rehmannia glutinosa Liboschitz (습해지역에서 식방풍과 지황의 생육양상)

  • Park, Sang-Gu;Kang, Dong-Kyoon;Chung, Sang-Hwan;Choi, Boo-Sull
    • Korean Journal of Medicinal Crop Science
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    • v.7 no.3
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    • pp.162-166
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    • 1999
  • In 1998, there was heavy precipitation of 60 days with amount of 1,042mm from June to September in Andong. Upland crop was severely injured by excessive moisture(EM) on poor drainage field. In this trial, effects of EM on the growth characteristics and yield in Peucedanum japonicum Thunberct and Rehmannia glutinosa Liboschitz was investigated. In Peucedanum japonicum Thunberct, growth of root was decreased more severely than that of aerial part by EM injury, and fresh weight of root per ha was decreased by $21{\sim}80%$ at different degree of EM injury compared to that of normal growth. In Rehmannia glutinosa Liboschitz, percent of withered plant was $10{\sim}52%$ at different degree of EM injury. and rhizome's number per plant and diameter was extremely decreased by EM injury. Fresh weight of rhizomes per ha was decreased by $19{\sim}83%$ at different degree of EM injury compared to that of normal growth.

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Effect of Virus-free Plant and Subsoiling Reversion Soil for Reduction of Injury by Continuous Cropping of Sweet Potato (고구마 연작장해 경감을 위한 바이러스 무병묘 재배와 심토반전 효과)

  • Song, Hae-Ahn;Kim, Kab-Cheol;Lee, Seung-Yeob
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.3
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    • pp.254-261
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    • 2012
  • To reduce the injury by continuous cropping of sweet potato (Ipomoea batatas (L.) Lam.), the farmer's plant and virus-free plant were cultivated with the density of $70{\times}25cm$ (June 10, 2011) in continuous cropping soil (CCS) and subsoiling reversion soil (SRS). Fertilizer was applied at the rates of 55-63-156 $kg\;ha^{-1}$ ($N-P_2O_5-K_2O$) and 10 $ton\;ha^{-1}$ of cattle manure in CCS, and it was applied the 50% increased cattle manure compost and nitrogen in DRS. Symptoms of viral infection were revealed in the farmer's plant at 30 days after planting, but there were no symptoms in virus-free plant. The yield of virus-free plant was more increased 15% and 10.5% than that of farmer's plant in DRS and CCS, respectively. The yield of sweetpotato in SRS was more increased 8.8% and 3.2% in farmer's plant and virus-free plant compared to CCS, respectively. In DRS, the rate of marketable tuber of virus-free plant was increased by 80% compared to the farmer's plant (60.1%). The virus-free plant was produced the tuber with more brilliant peel color and well-formed shape compared to the farmer's plant. The increased yield of virus-free plant and in SRS soil condition showed a positive relationship (p=0.05) with the number of leaf per plant at 30 days and the number of branch per plant at 120 days after planting. The results showed that the early growth after planting was very important for the development of storage root. Therefore, the deep-subsoil reversion and cultivation of virus-free plant could be reduced the injury by continuous cropping of sweet potato, and increased farm income.

Ga-mi-Yuk-Mi-Jihwang-Tang Ameliorates LPS-injected acute Liver Injury via Regulation of Sirtuin6 in Inflammasome Triggered-pyroptosis Using Mice Model

  • 임수아;조명래;김태수;성수희;김보람;최경민;정진우
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.114-114
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    • 2022
  • Excessive endogenous endotoxin, especially lipopolysaccharide (LPS) reflux from gastrointestinal (GI) tract to the liver tissue is one of the most serious reasons of severe and acute liver injury which is mainly mediated by Kupffer cell activations. However, there is no clear molecular clues to explain the exact pathophysiological mechanism and effective drugs available till nowadays. We aimed to comprehend the pathophysiological features of LPS-induced liver injury and evaluate the efficacies of potential therapeutic drug, Ga-mi-Yuk-Mi-Jihwang-Tang (GYM), which is composed of herbal plants. GYM remarkably caused to normalize hepatic inflammation and oxidations against LPS-induced liver injury by evidence of serum liver enzymes, histopathological analysis, both hepatic protein and gene expression levels of pro-inflammatory cytokines, nitric oxide levels, and hepatic tissue levels of reactive oxygen species (ROS) levels, malondialdehyde (MDA), and 4-hydroxyneoneal, respectively. To assess molecular events in the hepatic tissue, we further found hepatic Sirtuin6 (Sirt6) levels were considerably depleted by LPS injection with aberrant alterations of Nrf2/HO-1 signaling pathways, whereas administration with GYM notably exerted to normalize these abnormalities. Our results exhibited that GYM would be one of target drug to diminish hepatic inflammation as well as oxidative stress by regulation of hepatic Sirt6 levels.

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Salt Injury and Overcoming Strategy of Rice (수도의 염해와 대책)

  • 이승택
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.s02
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    • pp.66-80
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    • 1989
  • Salt injury in rice is caused mainly by the salinity in soil and in the irrigated water, and occasionaly by salinity delivered through typhoon from the sea. The salt concentration of rice plants increased with higher salinity in the soil of the rice growing. The climatic conditions, high temperature and solar radiation and dry conditions promote the salt absorption of rice plant in saline soil. The higher salt accumulation in the rice plant generally reduces the root activity and inhibits the absorption of minerals of rice plant, resulting the reduction of photosynthesis. The salt damages of rice plant, however, are different from different growth stage of rice plants as follows: 1. Germination of rice seed was slightly delayed up to 1.0% of salt concentration and remarkably at 1. 5%, but none of rice seeds were germinated at 2.5%. This may be due to the delayed water uptake of rice seeds and the inhibition of enzyme activity, 2. It was enable to establish rice seedlings at seed bed by 0.2% of salt concentration with some reduction of leaf elongation. The increasing of 0.3% salt concentration caused to the seedling death with varietal differences, but most of seedlings were death at 0.4% with no varietal differences. 3. Seedlings grown at the nursery over 0.1% salt, gradually reduced in rooting activity after transplanting according to increasing the salt concentration from 0.1% up to 0.3% of paddy field. However, the seedlings grown in normal seed bed showed no difference in rooting between varieties up to 0.1% but significantly different at 0.3% between varieties, but greatly reduced at 0.5% and died at last in paddy after transplanting. 4. At panicle initiation stage, rice plant delayed in heading by salt damage, at meiotic stage reduced in grains and its filling rate due to inhibition of glume and pollen developing, and salt damage at heading stage and till 3 weeks after heading caused to reduction of fertilization and ripening rate. In viewpoint of agricultural policy the overcoming strategy for salt injury is to secure sufficient water source. Irrigation and drainage systems as well as underground drainage is necessary to desalinize more effectively. This must be the most effective and positive way except cost. By cultural practice, growing the salt tolerant variety with high population could increase yield. The intermittent irrigation and fresh water flooding especially at transplanting and from panicle initiation to heading stage, the most sensitive to salt injury, is important to reduce the salt content in saline soil. During the off-cropping season, plough and rotavation with flooding followed by drainage, or submersion and drainage with groove could improve the desalinization. Increase of nitrogen fertilizer with more split application, and soil improvement by lime, organic matter and forign soil addition, could increase the rice yield. Shift of trans-planting is one of the way to escape from the salt injury.

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Studies on Reduction of Quinclorac Phytotoxicity in Ginseng Growth (인삼에 대한 제초제 Quinclorac의 약해 경감 연구)

  • 이일호;김명수
    • Journal of Ginseng Research
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    • v.20 no.1
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    • pp.101-105
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    • 1996
  • Several attempts have been made to protect crops against injury from herbicide quinclorac residue in soil. In this experiment, a selection of suitable crops for bioassay of the residue and a reduction of phytotoxicity by treatment with active carbon were carried out to prevent or to counteract the phytotoxicity. Cucumber (Cuumts satims) and kidney bean (Phaseolus Mgaris) were the suitable indicator plant in points of a sensitivity to the herbicide residue and an easy cultivation. The phytotoxicity was able to be observed at 20 and 30 days after seeding on kidney bean and cucumber respectively. In pot trials, application of the active carbon at 50 kg/10a protected effectively the 2-year-old ginseng plant from the injury in a paddy soil where the herbicide had been treated at 3 g a. i./10a.

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