• Journal of Forest and Environmental Science
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• v.37 no.4
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• pp.304-308
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• 2021

Iris koreana (Iridaceae) is an endangered plant native to Korea. In order to develop an in vitro propagation method, we investigated the effect of 2,4-dichlorophenoxy acetic acid (2,4-D) and a-naphthalene acetic acid (NAA) on callus induction in different I. koreana tissues. In addition, we also investigated the effect of 2,4-D and Benzyl aminopurine (BA) treatments on adventitious shoot induction in viable calli and the effect of indole-3-butyric acid (IBA) on root formation in viable shoots. We found that callus production was highest with 1.0 mg/L NAA (94.4% cultured rhizome explants), and adding low concentrations of 2,4-D to BA containing media significantly increased the frequency of shoot primordial formation. The best rooting results were obtained with 1.0 mg/L IBA, on which 98% of regenerated shoots developed roots and produced an average of 7.4 roots within 45 days. This in vitro propagation protocol will be useful for conservation, as well as for mass propagation.

• Korean Journal of Plant Resources
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• v.28 no.3
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• pp.350-356
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• 2015

Abeliophyllum distichum is a monotypic taxon of Oleaceae and endemic to Korea. A comprehensive study on embryogeny and fruit and seed coat ontogeny in Abeliophyllum was carried out via microtome and light microscopy. The fertilization occurs during mid– to late April and embryo matures by early July. The embryo development follows the general fashion from globular embryo – transition embryo – heart shaped embryo – torpedo embryo – walking-stick embryo to mature embryo. The pericarp clearly differentiates into three histological zones: exocarp, mesocarp, and endocarp. The young seed comprises 10-12 cells thick seed coat and the mature seed coat comprises an exotesta, 6-8 mesotesta and an endotesta. Any crystals, phenolic-like compounds, idioblasts, and the sclereids are not found in pericarp as well as seed coat. An overall development confirms Solanade type of embryogenesis in Abeliophyllum. The endocarp becomes more prominent in mature fruit and all the layers of endocarp are highly lignified. On the basis of mechanical layer the seed coat is of exotestal type.

• Horticultural Science & Technology
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• v.34 no.3
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• pp.372-382
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• 2016

In this study, we investigated the effect of seed collection stage and alternating temperature on the germination of Deutzia paniculata (Hydrangeaceae). The seeds were collected from naturally growing D. paniculata populations in Mt. Palgong, Gyeonsangbuk-do, Korea every two weeks over a three month period from August through October, 2014, totaling five collections. We examined seed characteristics such as seed size (length and width) and weight, and germination percentages for each collection stage under four different germination temperature regimes. We also examined germination after 30 and 60 days of cold moist stratification. Each collection phase had a different germination percentage and the lowest germination percentage was observed in the seeds from phase I (average GP of 15.7%). The germination percentage was similar at the later collection phases and in the higher temperatures suggesting that later collection times and higher temperatures are optimal for D. paniculata germination. We found that the optimum collection stage with high viability and germination percentage of for D. paniculata was during the middle of the September.

• Journal of Plant Biotechnology
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• v.7 no.2
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• pp.135-141
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• 2005

Protocols for in vitro conservation was developed for Coleus forskohlii. Plants maintained both in field served as explant source. Shoot tips and single node cuttings were used to optimize protocols for in vitro multiplication. MS basal medium supplemented with $0.54\;{\mu}M$ naphthalene acetic acid (NAA) and $8.87\;{\mu}M$ benzy-ladenine (BA) induced multiple shoots in shoot tips and nodes. Shoot multiplication was amplified with a gradual decrease of BA concentration, leading to its final omission after 4 months. Concomitant rooting on multiplication media enabled successful establishment extra vitrum. For in vitro conservation studies, experiments were carried out with 2-3 week maintained in vitro plants under standard and reduced culture conditions (SCC, RCC). In vitro plants could be successfully conserved in full strength MS medium (FMS) under SCC for 6 months without subculture with full potential to regenerate, producing viable shoots and nodes. The root production remained unaffected due to conservation, showing high rooting activity in mannitol and low temperature treatments. Preset low temperature (15 and $10^{\circ}C$) and reduction in media constituents does not appear to favour conservation, although the former accomplished conservation levels equal to (FMS) under SCC.

• Korean Journal of Environment and Ecology
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• v.30 no.5
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• pp.896-907
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• 2016

The habitat characteristics of Aconitum austrokoreense Koidz. were investigated to provide basic data for conservation and restoration. The altitude ranged from 260m to 728m with inclinations of $4-39^{\circ}$. As a result of vegetation survey within natural populations, a total of 147 taxa were identified inside 20 quadrates in 7 natural habitats. The importance value of Aconitum austrokoreense was 23.0% based on the coverage and frequency of the herbaceous layer and sat is more dominant. Species diversity was 1.23, and dominace and evenness were found to be 0.22 and 0.77, respectively. The soil type were silty loam (four sites), sandy loam (two sites) and loam (one site). The average field organic matter was 7.83% and the total nitrogen and pH were 0.35, and 5.69. Correlation analysis between environmental factors and population characteristic showed the correlations between chlorophyll content and slope, flowering rate and soil humidity. Conservation on habitats conditions is important for stable maintenance the flora and the surrounding area including habitats has to be designated a protection area for habitats conservation.

• Journal of Environmental Impact Assessment
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• v.6 no.2
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• pp.153-164
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• 1997

This study was conducted to develop the new Environmental Impact Assessment techniques for the grading of rareness in plant. 17 EISs which were submitted in 1996 were analyzed to know the problems in existent EIA techniques for the conservation of rare plants. Category of rare plant was reevaluated and evaluation technique for the grading of rareness in plant was developed. The results obtained from this study were as follows: 1. The evaluation technique of rareness in plant was not reflected in the current preparation provision for EIS. And t his fact also appeared in most of the EIS which were reexamined in this study. 2. The category of rare species which have been considered as the subject of protection in EIA have to be enlarged to more than legally protected species designated by MOE. 3. The taxonomic characteristics and status of species, characteristics of population, geographical characters, extent of threat and its possibility by man resulted from the habitat loss were investigated. Each item was endowed scores from 1 to 5 and all the scores were summed, and then this value was used to evaluate the order of conservation. 4. Conservation measures for the rare plants have to be applied according to the levels of rareness grade, and they are absolute conservation, in situ conservation, transplanting and ex situ conservation, post observation and none by the levels from A to E. 5. Considering the phenology of rare plants, investigation for the rare plants have to be made a1 least three times. 6. Size of the MVP(Minimum Viable Population), pollination ecology also have to be considered for the conservation of rare plants. And in the case of adopting ex situ conservation measure, the site and methods for the conservation of rare plants have to be proposed in detail.

• Journal of Ecology and Environment
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• v.33 no.4
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• pp.377-389
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• 2010

Understanding the factors relevant to endangerment and the patterns of habitat locations in relation to protected areas is critically important for the conservation of rare species. Although 64 plant species have recently been listed as endangered species in Korea, this information has, until now, not been available, making appropriate management and conservation strategies impossible to devise. Thus, we collected information on potentially threatening factors, as well as information on the locations in which these species were observed. The potentially threatening factors were classified into seven categories. National parks, provincial parks, ecosystem conservation areas, and wetland conservation areas were defined as protected conservation areas. Korean digital elevation model data, along with the maps of all protected areas were combined with the maps of endangered plant species, and analyzed via Geographic Information Systems (GIS). Excluding the category of "small population", endangered plant species in Korea were associated more frequently with extrinsic factors than intrinsic factors. Considering land surface only, all conservation areas in Korea totaled 4.9% of the land, far lower than International Union for Conservation of Nature and Natural Resources (IUCN)'s 10% coverage target. At the species level, 69% of the endangered plant species were detected in conservation areas, mostly in national parks. However, this result demonstrates that 31% of endangered species inhabit areas outside the conservation zones. Furthermore, at the habitat level, a large proportion of endangered species were found to reside in unprotected areas, revealing "gaps" in protected land. In the face of rapid environmental changes such as population increases, urbanization, and climate changes, converting these gap areas to endangered species' habitats, or at least including them in habitat networks, will help to perpetuate the existence of endangered species.

• Korean Journal of Plant Resources
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• v.28 no.4
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• pp.475-486
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• 2015

This survey was conducted in order to investigate the vascular plants of Hak Reservoir Valley in the DMZ Area, Korea. The vascular plants were collected six times (from April 2014 to November 2014) and consisted a total of 238 taxa; 69 families, 172 genera, 206 species, 2 subspecies, 26 varieties and 4 forms. The observed rare plant species as designated by Korea Forest Service were 3 taxa including Carex capricornis Meinsh. ex Maxim., Acorus calamus L., Penthorum chinense Pursh. Endemic plant species were 2 taxa including Salix koriyanagi Kimura, Clematis brachyura Maxim. 17 taxa were listed as specific plant species based on phytogeographical in the investigated area. 25 taxa were recorded as naturalized plants, and their Naturalization Ratio and Urbanization Index were recorded as 10.46%, and 7.78%, respectively. Of the 238 taxa listed; 105 were pasturing plants, 92 edible plants, 76 medicinal plants, 24 ornamental plants, 6 stain plants, 4 timber plants, 2 fiber plants, 2 industrial plants and 55 unknown plants.

• Korean Journal of Plant Taxonomy
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• v.42 no.2
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• pp.116-125
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• 2012

The distribution, vegetation characteristics and an assessment of the conservation status of the Korea endemic species Coreanomecon hylomeconoides Nakai were investigated to collect biological basic data to formulate a conservation strategy. According to the distribution map of C. hylomeconoides based on the literature, specimen information, and local field surveys, natural populations of C. hylomeconoides are intensively distributed in the Southern regions of the peninsula, around Gyeongsangnam-do and Jeollanan-do. C. hylomeconoides was distributed in the middle and low slope adjacent to a valley. The altitude ranged from 227 m to 744 m, with inclinations of $5-10^{\circ}$. As a result of a vegetation survey within natural populations, a total of 238 taxa were identified from 29 quadrates in 8 natural populations. The importance value of C. hylomeconoides is 25.34% based on the coverage and frequency of the herbaceous layer with in the populations. The species diversity of the occurrence of the species in 8 natural populations was 1.52, while the averages of species evenness and the dominance values were calculated to be 0.83 and 0.17, respectively. As a result of assessing the conservation status through IUCN Red List criteria, C. hylomeconoides was evaluated as Near Threatened (NT). Conservation strategies are also discussed for the sustainable conservation of C. hylomeconoides.

• Journal of the Korean association of regional geographers
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• v.21 no.2
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• pp.355-363
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• 2015

DMZ(Demilitarized Zone) created as part of the Armistice Agreement is important zone in the way that historic and eco-cultural value as well as military and political image of this are remarkable. Furthermore, the concern of DMZ has been rising at home and abroad. Even though various studies on this subject have been constantly spurring according to the significance, the practical researches on spatial range of DMZ are very weak. The Purpose of this research is to show the factual spatial extent of DMZ from the mouth of the Imjingang River in the west to the town of Goseong in the east. This study proved the area of corresponding DMZ is approximately $570km^2$ contrary to the previous studies and claims it is difficult to measure the actual length of MDL(Military Demarcation Line) regarded as 155mile in this situation of Korea peninsula.