• Proceedings of the Korean Society of Crop Science Conference
• /
• 한국작물학회 2022년도 춘계학술대회
• /
• pp.104-104
• /
• 2022

• Plant Resources
• /
• 제8권3호
• /
• pp.286-292
• /
• 2005

Shoot induction system was developed in the recalcitrant plant species, Brassica rapa ssp. rapifera by using optimum selection of profit organ, phytohormone combination, seedling age and kind of culture container. Out of in vitro cultured leaf segment, petiole, hypocotyl, and cotyledon with petiole, only cotyledon with petiole derived from 4 day-old seedlings induced multiple shoot. The optimum combination of auxin and cytokinin for the multiple shoot induction was MS medium containing 5mg/L BA and 0.5mg/L NAA. The major factors for multiple shoot propagation were part of plant organ, age of seedling, and ratio of auxin and cytokinin. In addition, shoot regeneration was promoted in the 100ml Erlenmeyer flask compared with the $90mm{\times}20mm$ Petri-dish. The induced shoots formed roots easy on MS medium containing 0.1mg/L IBA and the whole plants were successfully cultivated in soil.

• Journal of Biosystems Engineering
• /
• 제39권2호
• /
• pp.87-95
• /
• 2014

Purpose: The purpose of this study was to design a variable frequency LED light system for plant factory which combined red, blue, green, white, and UV lights and controlled the ratio of the light wavelength. In addition, this study evaluated the performance of each combination of LED to verify the applicability. Methods: Four combinations of LED (i.e. Red+Blue, Red+Blue+Green, Red+Blue+White, Red+Blue+UV) were designed using five types of LED. The system was designed to control the duty ratio of each wavelength of LED by 1% interval from 0~100%, the pulse by 1Hz interval from 1~20kHz. Response characteristics of the control system, spectral distribution of each combination, light uniformity and uniformity ratio were measured to test the performance of the system. Results: Clean waveforms were measured from 10Hz to 10kHz regardless of duty ratio. Frequency distortion was observed within 5% of inflection point at frequencies above 10kHz regardless of duty ratio, but it was judged negligible. Spectra showed a normal distribution, and maximum PPF with duty ratio of 100% was $271.4{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ for the Red+Blue combination. PPF of the Red+Blue+Green combination was $258.9{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, and that of the Red+Blue+White combination was $273.9{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. PPF of the Red+Blue+UV combination was $267.7{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Uniformity ratio for the area excepting border showed 0.90 for the Red+Blue and Red+Blue+White combinations, 0.87 for the Red+Blue+Green combination, and 0.88 for the Red+Blue+UV combination. The light was irradiated evenly at the area excepting border, so it was suitable for plant growing. Conclusions: From the results of this study, response characteristics of the control system, spectral distribution of each combination, light uniformity and uniformity ratio were suitable for applying into the plant factory.

• Journal of Korean Society of Water and Wastewater
• /
• 제38권4호
• /
• pp.233-242
• /
• 2024

This study evaluates the potential of various coagulants to enhance the efficiency of total phosphorus removal facilities in a sewage treatment plant. After analyzing the existing water quality conditions of the sewage treatment plant, the coagulant of poly aluminium chloride was experimentally applied to measure its effectiveness. In this process, the use of poly aluminium chloride and polymers in various ratios was explored to identify the optimal combination of coagulants. The experimental results showed that the a coagulants combination demonstrated higher treatment efficiency compared to exclusive use of large amounts of poly aluminium chloride methods. Particularly, the appropriate combination of poly aluminium chloride and polymers played a significant role. The optimal coagulant combination derived from the experiments was applied in a micro flotation method of real sewage treatment plant to evaluate its effectiveness. This study presents a new methodology that can contribute to enhancing the efficiency of sewage treatment processes and reducing environmental pollution. This research is expected to make an important contribution to improving to phosphorus remove efficiency of similar wastewater treatment plant and reducing the ecological impact from using coagulants in the future.

• Korean Journal of Oriental Medicine
• /
• 제14권1호
• /
• pp.113-116
• /
• 2008

Petiole explant of Glehnia littoralis Fr. Schmidt was in vitro cultured MS plant medium(DUCHEFA co.) supplemented with various plant growth regulators and examined to find out their optimum combination and concentration for plantlet regeneration. We investigated optimal condition for efficient plant regeneration through adventitious shoot formation on MS plant medium with various kinds of plant growth regulators. Embryogenic calli and adventitious shoot formation were greatly influenced by plant growth regulators. Embryogenic calli induction showed a good response on MS medium supplemented with NAA and BA than others. Especially, combination of 1.0 mg/L NAA and 0.5 mg/L BA on MS medium led to the greatest frequency in adventitious shoot. The results suggest that plant regulator selection be important factor to achieve an efficient regeneration.

• Journal of Plant Biology
• /
• 제39권3호
• /
• pp.179-184
• /
• 1996

The purpose of this study is to obtain the most efficient combination of Agrobacterium tumefaciens strains and Ti plasmids for the construction of dicotyledonous plant vector system. Ti plasmid-curing A. tumefaciens A136 and KU12C3 were transformed with four kinds of Ti plasmids, pTiBo542, pTiA6, pTiKU12 and pTiAch5, respectively. The stems of 28 species of dicotyledonous plants were then inoculated with these transformants and examined for crown gall formation. The different combination of A. tumefaciens strains and Ti plasmids showed quite a difference in terms of the crown gall formation. Agrobacterium strins A136 and KU12C3 have a same plant host range in case that both strains harour the same kind of Ti plasmid, pTiBo542 or pTiAch5. However, the above-mentioned both strains have quite different host range in the event of containing the same Ti plasmid, pTiKU12 or pTiA6. In case that KU12C3 contains pTiA6 or pTiKU12, this strain has a wider plant host range than A136. The plant host range of pTiBo542 is the widest, followed by pTiA6, pTiKU12 and pTiAch5. Twelve plants among 28 tested plants are not transformed by any virulent Agrobacterium strains used in this study. In conclusion, A. tumefaciens KU12C3 and A136 harboring pTiBo542 showed the widest host range for transforming dicotyledonous plants. Also, it was acertained that the host range of Ti plasmids is affected by chromosomal level.

• The Plant Pathology Journal
• /
• 제25권3호
• /
• pp.256-262
• /
• 2009

The potential of. nonpathogenic Fusarium oxysporum strain Avr5, either alone or in combination with chitosan and Bion, for inducing defense reaction in tomato plants inoculated with F. oxysporum f. sp lycopersici, was studied in vitro and glasshouse conditions. Application Bion at concentration of 5, 50, 100 and $500{\mu}g$/ml, and the highest concentration of chitosan reduced in vitro growth of the pathogen. Nonpathogenic F. oxysporum Avr5 reduced the disease severity of Fusarium wilt of tomato in split plants, significantly. Bion and chitosan applied on tomato seedlings at concentration $100{\mu}g$ a.i./plant; 15, 10 and 5 days before inoculation of pathogen. All treatments significantly reduced disease severity of Fusarium wilt of tomato relative to the infected control. The biggest disease reduction and increasing tomato growth belong to combination of nonpathogenic Fusarium and Bion. Growth rate of shoot and root markedly inhibited in tomato plants in response to tomato Fusarium wilt as compared with healthy control. These results suggest that reduction in disease incidence and promotion in growth parameters in tomato plants inoculated with nonpathogenic Fusarium and sprayed with elicitors could be related to the synergistic and cooperative effect between them, which lead to the induction and regulation of disease resistance. Combination of elicitors and non-pathogenic Fusarium synergistically inhibit the growth of pathogen and provide the first experimental support to the hypothesis that such synergy can contribute to enhanced fungal resistance in tomato. This chemical could provide a new approach for suppression of tomato Fusarium wilt, but its practical use needs further investigation.

• Transactions of the Korean Society for Noise and Vibration Engineering
• /
• 제15권6호
• /
• pp.652-659
• /
• 2005

In this paper, seismic qualification analysis for the Plant control Panel is carried out to confirm the structural integrity under the seismic conditions represented by required response spectra(RRS). The finite element method(FEM) is used for the analysis and a mode combination method is adopted to obtain a more reliable spectrum analysis results. In addition, the experimental analysis is performed to compare the reliability of the analytical results. The analysis results shows that the plant control panel system is designed to have the dynamic rigidity with no resonance frequency below 33 Hz. The analytically calculated maximum stress of the plant control panel system is $36\%$ of the field strength of material, thus it can be shown that the system has a stable structure for the seismic load.

• Plant Biotechnology Reports
• /
• 제4권2호
• /
• pp.173-178
• /
• 2010

An efficient protocol for shoot regeneration was developed for sesame (Sesamum indicum L.) internodes using the transverse thin cell layer (tTCL) culture method. The frequency of shoot regeneration and the number of adventitious buds produced from regenerated shoots depend significantly on explant age, thickness of the tTCL sections, and the phytohormones supplemented to the culture medium. A combination of 6-benzyladenine (2.0 $mg\;l^{-1}$) and a-naphthaleneacetic acid (0.5 $mg\;l^{-1}$) was found to be the best phytohormone combination for shoot bud induction, with the maximum number of shoots obtained when the tTCL sections were 0.5-1.0 mm thick and derived from 4- to 6-week-old seedlings of sesame. Well-developed shoots were rooted on MS medium without phytohormones, and 80% of the regenerated plantlets were successfully established in soil.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 한국자원식물학회 2001년도 춘계 학술발표대회
• /
• pp.71-79
• /
• 2001