• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.58-58
• /
• 2017

The advent of next generation sequencing technology has elicited plenty of sequencing data available in agriculturally relevant plant species. For most crop species, it is too expensive to obtain the whole genome sequence data with sufficient coverage. Thus, many approaches have been developed to bring down the cost of NGS. Genotyping-by-sequencing (GBS) is a cost-effective genotyping method for complex genetic populations. GBS can be used for the analysis of genomic selection (GS), genome-wide association study (GWAS) and constructing haplotype and genetic linkage maps in a variety of plant species. For efficiently dealing with plant GBS data, the TASSEL-GBS pipeline is one of the most popular choices for many researchers. TASSEL-GBS is JAVA based a software package to obtain genotyping data from raw GBS sequences. Here, we describe application of GBS and bioinformatics pipeline of TASSEL-GBS for analyzing plant genetics data.

• Mycobiology
• /
• v.29 no.3
• /
• pp.154-159
• /
• 2001

Natural sclerotium formation in two species of Curvularia was observed. The sclerotia were spherical to elongated. The scanning electron microscopical observations of sclerotia revealed that the sclerotia were of two distinct layers of cells, outer loosely woven hyphae and inner contact layer of cells. Different lights, viz. red, blue, green, fluorescent or addition of culture filtrate of Sclerotium rolfsii in the medium did not affect sclerotium formation.

• Mycobiology
• /
• v.41 no.2
• /
• pp.116-119
• /
• 2013

Three Penicillium species have been isolated from insect specimens in Korea; Penicillium sp., P. steckii, and P. polonicum. Penicillium sp. (KNU12-3-2) was isolated from Lixus imperessiventris, while P. polonicum (KNU12-1-8) and Penicillium steckii (KNU12-2-9) were isolated from Muljarus japonicas and Meloe proscarabaeus, respectively. The identification was based on the morphological characteristics of the fungi and in internal transcribed spacer analysis. This is the first report on the isolation of these three species of Penicillium from insects in Korea.

• Korean Journal of Ecology and Environment
• /
• v.54 no.2
• /
• pp.102-107
• /
• 2021

The distribution pattern of species richness was determined by temperature. To examine the relationship between hemipteran richness and temperature, hemipteran species were collected using pitfall traps at six different oak forest sites with different annual mean temperatures in South Korea. Multiple linear regression analyses were conducted with mean annual temperature (MAT) and plant richness to evaluate differences in hemipteran richness. The influences of MAT and plant richness of study sites on hemipteran richness were examined by comparing three models (plant richness+MAT+MAT², plant richness+MAT, and MAT) or two models (plant richness+MAT and MAT). Hemipteran richness showed an inverse diversity pattern as a function of temperature, with higher species richness at lower temperature sites. Meanwhile, Aphididae showed a bell-shaped diversity pattern with the highest value at low medium temperatures. The regression analysis showed that hemipteran richness was affected by temperature and plant richness in their habitats.

• Proceedings of the National Institute of Ecology of the Republic of Korea
• /
• v.3 no.1
• /
• pp.23-31
• /
• 2022

The analysis of seven islands in Gogunsan archipelago, Korea with insect fauna and vascular plant flora was carried out based on a field survey conducted in May, July, and September. As a result, a total of 2,817 insect individuals including 264 species and 315 taxa of vascular plant were recorded. Bangchukdo the largest island among the seven islands showed the largest number both insect species and plant taxa. The similarity analysis suggested that the nearness of each island strongly affected the insect fauna and vascular plant flora on each island. In addition, there was significant correlation between the areas of each island and the numbers of insect species (Spearman's correlation coefficient=0.857, P-value=0.014). In the future, the results of this study can be used as data related to island ecology and conservation.

• Korean Journal of Plant Resources
• /
• v.36 no.3
• /
• pp.219-224
• /
• 2023

Melilotus dentatus (Waldst. & Kit.) Desf. is an invasive plant native to Europe, Russia, Uzbekistan, Mongolia and China. It was introduced to North America to create grazing areas for livestock; its growth has since become uncontrollable. Melilotus dentatus is now found in Namyang-eup, Hwaseong-si, Gyeonggi-do, South Korea as an alien plant. This species is morphologically similar to Melilotus suaveolens Ledeb. and Melilotus officinalis (L.) Lam. M. dentatus differs from other species based on its oblong leaflets, distinct teeth, two ovules, surface of the legume that is minutely reticulate-nerved or smooth, and seeds that are dark green to greenish brown. Here, we have provided a detailed description and photographs of the alien species M. dentatus as well as an updated key to five Melilotus taxa studied, which will help prepare a continuous monitoring and management plan.

• ALGAE
• /
• v.19 no.3
• /
• pp.257-270
• /
• 2004

In order to clarify the structure and seasonal dynamics of warm tolerant benthic marine algal community in Korea, the species composition and biomass of marine algae at the discharge canal of Uljin nuclear power plant on the East Coast of Korea were investigated seasonally from February 1992 to October 2000. 107 species of marine algae were found at the discharge canal during the past nine years. In general, the number of species observed was abundant in spring or summer and less in autumn or winter. 27 species (4 blue-green, 5 green, 6 brown and 12 red algae) of marine algae occurred more than 1/ 6 frequency and thus can be categorized as warm tolerant species. Among these, one brown (Dictyota dichotoma) and four red algae (Gelidium amansii, Anphiroa ephedraea, Hydrolithon sargassi, Marginisporum crassissimum) are recorded as warm tolerant marine algae for the first time in Korea. Padina arborescens, Anphiroa zonata and Corallina pilulifera were common species found more than 75% frequency. Seasonal fluctuations of mean biomass were 0-1,330 g dry wt m^(-2) and dominant species in biomass were Corallina pilulifera (contribution to a total biomass proportion 34%), Anphiroa zonata (23%), Padina arborescens (18%) and Sargassum micracanthum (11%). The red algae appeared as predominant algal group at the discharge canal of Uljin nuclear power plant in the qualitative and quantitative aspects. The green algae such as Enterornorpha compressa appeared rather frequently at the discharge canal, but the biomass proportion was very low, in contrast to Kori nuclear power plant where there was definite green algal dominance. Differences in algal communities developed at the discharge canals of Uljin and Kori nuclear power plant on the East Coast of Korea, particularly biomass proportions of green algae, can probably be related to local environmental factors such as water velocity through the canal and natural seawater temperatures.

• The Plant Pathology Journal
• /
• v.31 no.3
• /
• pp.212-218
• /
• 2015

In this study, we developed a species-specific PCR assay for rapid and accurate detection of three Xanthomonas species, X. axonopodis pv. poinsettiicola (XAP), X. hyacinthi (XH) and X. campestris pv. zantedeschiae (XCZ), based on their draft genome sequences. XAP, XH and XCZ genomes consist of single chromosomes that contain 5,221, 4,395 and 7,986 protein coding genes, respectively. Species-specific primers were designed from variable regions of the draft genome sequence data and assessed by a PCR-based detection method. These primers were also tested for specificity against 17 allied Xanthomonas species as well as against the host DNA and the microbial community of the host surface. Three primer sets were found to be very specific and no amplification product was obtained with the host DNA and the microbial community of the host surface. In addition, a detection limit of $1pg/{\mu}l$ per PCR reaction was detected when these primer sets were used to amplify corresponding bacterial DNAs. Therefore, these primer sets and the developed species-specific PCR assay represent a valuable, sensitive, and rapid diagnostic tool that can be used to detect three specific pathogens at early stages of infection and may help control diseases.

• Journal of Ecology and Environment
• /
• v.42 no.2
• /
• pp.70-76
• /
• 2018

Background: Sasa borealis (Hack.) Makino, a clonal dwarf bamboo, is widespread in Korean forests. Although S. borealis is native to that country, its growth habit can cause considerable harm when occupying particular areas where it dominates and influences those forested communities. However, few reports have described the extent of its inhibitory effects on the vigor of co-existing plant species. Therefore, we investigated the distribution, abundance, and diversity of other plant species in the communities where this plant occurs in the east-central forests on the Korean Peninsula. Results: S. borealis was most commonly found at an elevational range of 800 to 1,200 m, on gentle, usually lower, and near valley northern slopes. Out of the 13 forest communities based on 447 forest stands that we surveyed, S. borealis was detected in eight communities, mostly where Quercus mongolica dominates. In particular, it was more common in late-successional mixed stands of Q. mongolica, other deciduous species, and the coniferous Abies holophylla. Because of their ability to expand rapidly in the forest, this plant covered more than 50% of the surface in most of our research plots. Species diversity declined significantly (F = 78.7, p = 0.000) as the abundance of S. borealis increased in the herb stratum. The same trend was noted for the total number of species (F = 18.1, p = 0.000) and species evenness (F = 91.5, p = 0.000). Conclusions: These findings clearly demonstrate that S. borealis is a weed pest and severely hinders species diversity. Authorities should be implementing various measures for ecological control to take advantage of declining chance after the recent synchronized massive flowering of S. borealis.

• The Korean Journal of Ecology
• /
• v.18 no.3
• /
• pp.307-321
• /
• 1995

Division of ecoregions having respective functions was attempted through quantitative and qualitative analysis on vegetation diversity, and heterogeneity and on soil environment of the study sites. Field research was carried out in a square of 81 ㎢ around Andongpo (126°38'E, 37°30'N), Kimpo-gun, Kyonggi provice. Conventional methods applied are as follows: classical syntaxonomy by the Zurich-Montpellier School, interpolation method to determine the degree of diversity, heterogeneity and distribution pattern of vegetation, and correlation analysis between soil properties and plant communities. 41 plant communities were identified and composed of 6 forests, 4 mantle and 31 herb communities including 6 saltmarsh plant communities. In a mesh, number of plant communities was highly correlated to the number of species. The highest number of plant community and species was 25 communities·km-2·mesh-1 and 381 species· km-2·mesh-1 ,and the highest value of vegetation heterogeneity was 28.1 species· community-1·mesh-1. Their lowest numbers were 4 communities·km-2·mesh-1. and 28 species·km-2·mesh-1. and 7 species·community-1·mesh-1, respectively. Contour map on vegetation diversity and heterogeneity enabled us to establish two regions; coastal and inland vegetation. Isoline 〔150〕,〔10〕and〔10〕and〔15〕on the species diversity, the community diversity and the vegetation heterogeneity, respectively, were regarded as ecolines in the study area. Cl- content was recognized as the most important factor from correlation analysis between soil properties. Ordination of sites indicated that the study area be divided into two edaphic types: inland and coastal habitats. It was considered that the extent of desalinization in soil played a major role in determining the species composition in the reclamed area. By matching edaphic division of habitats with division of vegetation structures, designation of ecoregion was endorsed. The approach of current study was suggested as an effective tool to implement an assessment of the vegetation dynamics by the disparity of natural environment and anthropogenic interferences.