• The Korean Journal of Pesticide Science
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• v.21 no.1
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• pp.68-74
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• 2017

An analytical method for detecting metamifop residue in paddy water, soil, and rice with high performance liquid chromatography (HPLC) was developed. Water was extracted with ethyl acetate before analyzing by HPLC. Soil residues were extracted with acetone under acidic condition and after purifying with $Extrelut^{(R)}$ NT, and silica SPE, the residue was analyzed by HPLC. For residue analysis in rice, the procedure involved extraction with acetone, purification with $Extrelut^{(R)}$ NT, partitioning between acetonitrile/hexane, purification with silica SPE cartridge, and analysis by HPLC. The limit of detection (LOD) was 1.0 ng, limit of quantitation (LOQ) was 3.0 ng, and method limit of quantitation (MLOQ) were 0.001 mg/L for paddy water, 0.01 mg/kg for rice and soil, respectively. Standard calibration curve shows linearity from 0.05 mg/kg to 5.0 mg/kg ($R^2=0.9999$). The recoveries in fortified paddy water were $91.3{\pm}3.5%$ (0.01 mg/L level) and $93.2{\pm}6.3%$ (0.05 mg/L level). The recoveries in fortified paddy soils were $92.5{\pm}4.0%$ (0.1 mg/kg level) and $92.7{\pm}4.0%$ (0.5 mg/kg level) in soil A, while, $102.3{\pm}4.4%$ (0.1 mg/kg level) and $98.9{\pm}7.9%$ (0.5 mg/kg level) in soil B, respectively. The recoveries in fortified rice were $93.0{\pm}6.9%$ (0.1 mg/kg level) and $85.0{\pm}3.5%$ (0.5 mg/kg level). This method was proved to be effective and can be used to determine the metamifop residue in paddy water, paddy soil, and rice.

• Korean Chemical Engineering Research
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• v.43 no.4
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• pp.487-494
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• 2005

Nowadays a large amount of wastewater containing metal working fluids and cleaning agents is generated during the cleaning process of parts working in various industries of automobile, machine and metal, and electronics etc. In this study, aqueous or semi-aqueous cleaning wastewater contaminated with soluble or nonsoluble oils was treated using ultrafiltration system. And the membrane permeability flux and performance of oil-water separation (or COD removal efficiency) of the ultrafiltration system employing PAN as its membrane material were measured at various operating conditions with change of membrane pore sizes and soil concentrations of wastewater and examined their suitability for wastewater treatment contaminated with soluble or insoluble oil. As a result, in case of wastewater contaminated with soluble oil and aqueous or semi-aqueous cleaning agent, the membrane permeability increased rapidly even though COD removal efficiency was almost constant as 90 or 95％ as the membrane pore size increased from 10 kDa to 100 kDa. However, in case of the wastewater contaminated with nonsoluble oil and aqueous or semi-aqueous cleaning agent, as the membrane pore size increased from 10 kDa to 100 kDa and the soil concentration of wastewater increased, the membrane permeability was reduced rapidly while COD removal efficiency was almost constant. These phenomena explain that since the membrane material is hydrophilic PAN material, it blocks nonsoluble oil and reduces membrane permeability. Thus, it can be concluded that the aqueous or semi-aqueous cleaning solution contaminated with soluble oil can be treated by ultrafiltration system with the membrane of PAN material and its pore size of 100 kDa. Based on these basic experimental results, a pilot plant facility of ultrafiltration system with PAN material and 100 kDa pore size was designed, installed and operated in order to treat and recycle alkaline cleaning solution contaminated with deep drawing oil. As a result of its field application, the ultrafiltration system was able to separate aqueous cleaning solution and soluble oil effectively, and recycle them. Further more, it can increase life span of aqueous cleaning solution 12 times compared with the previous process.

• Korean Chemical Engineering Research
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• v.50 no.6
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• pp.1002-1007
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• 2012

The considerable portion of energy demand has been satisfied by the combustion of fossil fuel and the consequent $CO_2$ emission was considered as a main cause of global warming. As a technology option for $CO_2$ emission mitigation, absorption process has been used in $CO_2$ capture from large scale emission sources. To set up optimal operating parameters in $CO_2$ absorption and solvent regeneration units are important for the better performance of the whole $CO_2$ absorption plant. Optimal operating parameters are usually selected through a lot of actual operation data. However theoretical approach are also useful because the arbitrary change of process parameters often limited for the stability of process operation. In this paper, a theoretical approach based on vapor-liquid equilibrium was proposed to estimate optimal operating conditions of $CO_2$ absorption process. Two $CO_2$ absorption processes using 12 wt% aqueous $NH_3$ solution and 20 wt% aqueous MEA solution were investigated in this theoretical estimation of optimal operating conditions. The results showed that $CO_2$ loading of rich absorbent should be kept below 0.4 in case of 12 wt% aqueous $NH_3$ solution for $CO_2$ absorption but there was no limitation of $CO_2$ loading in case of 20 wt% aqueous MEA solution for $CO_2$ absorption. The optimal regeneration temperature was determined by theoretical approach based on $CO_2$ loadings of rich and lean absorbent, which determined to satisfy the amount of absorbed $CO_2$. The amount of heating medium at optimal regeneration temperature is also determined to meet the difference of $CO_2$ loading between rich and lean absorbent. It could be confirmed that the theoretical approach, which accurately estimate the optimal regeneration conditions of lab scale $CO_2$ absorption using 12 wt% aqueous $NH_3$ solution could estimate those of 20 wt% aqueous MEA solution and could be used for the design and operation of $CO_2$ absorption process using chemical absorbent.

• Korean journal of applied entomology
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• v.51 no.3
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• pp.235-243
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• 2012

Population dynamics of the American serpentine leafminer, Liriomyza trifolii (Burgess), were observed and modeled in order to compare the effects of air and tomato leaf temperatures inside a greenhouse using DYMEX model builder and simulator (pre-programed module based simulation programs developed by CSIRO, Australia). The DYMEX model simulator consisted of a series of modules with the parameters of temperature dependent development and oviposition models of L. trifolii were incorporated from pre-published data. Leaf surface temperatures of cherry tomato leaves (cv. 'Koko') were monitored according to three tomato plant positions (top, > 1.8 m above the ground level; middle, 0.9 - 1.2 m; bottom, 0.3 - 0.5 m) using an infrared temperature gun. Air temperature was monitored at the same three positions using a self-contained temperature logger. Data sets for the observed air temperature and average leaf surface temperatures were collected (top and bottom surfaces), and incorporated into the DYMEX simulator in order to compare the effects of air and leaf surface temperature on the population dynamics of L. trifolii. The initial population consisted of 50 eggs, which were laid by five female L. trifolii in early June. The number of L. trifolii larvae was counted by visual inspection of the tomato plants in order to verify the performance of DYMEX simulation. The egg, pupa, and adult stage of L. trifolii could not be counted due to its infeasible of visual inspection. A significant positive correlation between the observed and the predicted numbers of larvae was found when the leaf surface temperatures were incorporated into the DYMEX simulation (r = 0.97, p < 0.01), but no significant positive correlation was observed with air temperatures(r = 0.40, p = 0.18). This study demonstrated that the population dynamics of L. trifolii was affected greatly by the leaf temperatures, though to little discernible degree by the air temperatures, and thus the leaf surface temperature should be for a consideration in the management of L. trifolii within cherry tomato greenhouses.

• Korean journal of applied entomology
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• v.53 no.4
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• pp.375-380
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• 2014

The sweetpotato whitefly (SPW), Bemisia tabaci Gennadius, is a major pest in tomato greenhouses on Jeju Island because they transmit viral diseases. To develop practical sampling methods for adult SPWs, yellow-color sticky traps were used in commercial tomato greenhouses throughout the western part of Jeju Island in 2011 and 2012. On the basis of the size and growing conditions in the tomato greenhouses, 20 to 30 traps were installed in each greenhouse for developing a sampling plan. Adult SPWs were more attracted to horizontal traps placed 60 cm above the ground than to vertical trap placed 10 cm above the plant canopy. The spatial patterns of the adult SPWs were evaluated using Taylor's power law (TPL) and Iwao's patchiness regression (IPR). The results showed that adult SPWs were aggregated in each surveyed greenhouse. In this study, TPL showed better performance because of the coefficient of determination ($r^2$). On the basis of the fixed-precision level sampling plan using TPL parameters, more traps were required for higher precision in lower SPW densities per trap. A sequential sampling stop line was constructed using TPL parameters. If the treatment threshold was greater than 10 maximum adult SPWs on a trap, the required traps numbered 15 at a fixed-precision level of 0.25. In estimating the mean density per trap, the proportion of traps with two or more adult SPWs was more efficient than whole counting: ${\ln}(m)=1.19+0.90{\ln}(-{\ln}(1-p_T))$. The results of this study could be used to prevent the dissemination of SPW as a viral disease vector by using accurate control decision in SPW management programs.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.4
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• pp.460-472
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• 1995

This study was aimed to provide understanding on the eco-physiological response of barley tillers as affected by difference in seeding date. Yield and yield contribution rates of tillers were investigated with the data of field experiments in the former Wheat and Barley Research Institute of Suwon, Korea from September 1982 to July 1984. When barley was sowed 15 days earlier than or at the locally recommended sowing date (i.e. October 5), it produced more mainstem leaves than the that sowed 15 days later than the local recommendation. The effective tillers (i.e. ear-bearing tillers) were observed in concurrently occurring leaves up to 10/0 (abbreviation of the 10th mainstem leaf) from early and optimum sowed crops, while 9/0 from late sowed crops. Tillering followed the production rule of Gatayama(l952) which has the linear leaf appearance pattern. Early sowing produced more effective tillers, but the highest production of effective ears (i.e. ears heavier than 0.505g) was noticed in sowing at the recommended date. The tillers in the axil of first leaf in mainstem (abbreviated as 1) produced more effective ears than other mainstem tillers (i.e. primary tillers such as C, 2, 3 and 4). The tillers from the axil of first leaf, whether they were primary or secondary, always performed better in the production of effective tillers, grains per ear, grain weight, and grain yield per ear than those from the axil of coleoptile or prophyll. Other tillers from subsequent leaves were also inferior in production with the order of their appearance, thus making first leaf tillers as the best performer(e.g. 1 against C, 2, 3, 4 of primary tillers, 11 and 21 against 1P, 12, 13, 2P, 22 of secondary tillers). Even though the first leaf tiller from the first mainstem leaf(i.e. 11) emerged at a same time with the fourth mainstem leaf tiller(i.e. 4), it was always a better producer of ears and grains. The above observations of hierarchy among tillers were persistent irrespective of cropping conditions and treatments. Sowing at the recommended date produced more effective tillers and grains per ear than early or late sowing. In early and late sowings, more grains per effective ear were observed by early sowing, whereas more grains per ear were produced by late sowing. The order of performance in production of effective tillers and ears per plant was as follows: optimum > early > late sowings. In optimum sowing the mainstems were the highest in grain weight per ear, while the first leaf tillers were the ones in early sowing probably due to winter damage on mainstem ears. Yield contribution by the tillers was greater with the following order irrespective of sowing dates: mainstem, 1, 2, 3, C, 11, 12 and Cl. The contribution of CP, 2P, 21 and 31 varied with sowings.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.8-9
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• 2017

Soybean yield has been low and unstable in Japan and other areas in East Asia, despite long history of cultivation. This is contrasting with consistent increase of yield in North and South America. This presentation tries to describe perspective of breaking stagnation of soybean yield in East Asia, considering the factors of the different yields between regions. Large amount of rainfall with occasional dry-spell in the summer is a nature of monsoon climate and as frequently stated excess water is the factor of low and unstable soybean yield. For example, there exists a great deal of field-to-field variation in yield of 'Tanbaguro' soybean, which is reputed for high market value and thus cultivated intensively and this results in low average yield. According to our field survey, a major portion of yield variation occurs in early growth period. Soybean production on drained paddy fields is also vulnerable to drought stress after flowering. An analysis at the above study site demonstrated a substantial field-to-field variation of canopy transpiration activity in the mid-summer, but the variation of pod-set was not as large as that of early growth. As frequently mentioned by the contest winners of good practice farming, avoidance of excess water problem in the early growth period is of greatest importance. A series of technological development took place in Japan in crop management for stable crop establishment and growth, that includes seed-bed preparation with ridge and/or chisel ploughing, adjustment of seed moisture content, seed treatment with mancozeb+metalaxyl and the water table control system, FOEAS. A unique success is seen in the tidal swamp area in South Sumatra with the Saturated Soil Culture (SSC), which is for managing acidity problem of pyrite soils. In 2016, an average yield of $2.4tha^{-1}$ was recorded for a 450 ha area with SSC (Ghulamahdi 2017, personal communication). This is a sort of raised bed culture and thus the moisture condition is kept markedly stable during growth period. For genetic control, too, many attempts are on-going for better emergence and plant growth after emergence under excess water. There seems to exist two aspects of excess water resistance, one related to phytophthora resistance and the other with better growth under excess water. The improvement for the latter is particularly challenging and genomic approach is expected to be effectively utilized. The crop model simulation would estimate/evaluate the impact of environmental and genetic factors. But comprehensive crop models for soybean are mainly for cultivations on upland fields and crop response to excess water is not fully accounted for. A soybean model for production on drained paddy fields under monsoon climate is demanded to coordinate technological development under changing climate. We recently recognized that the yield potential of recent US cultivars is greater than that of Japanese cultivars and this also may be responsible for different yield trends. Cultivar comparisons proved that higher yields are associated with greater biomass production specifically during early seed filling, in which high and well sustained activity of leaf gas exchange is related. In fact, the leaf stomatal conductance is considered to have been improved during last a couple of decades in the USA through selections for high yield in several crop species. It is suspected that priority to product quality of soybean as food crop, especially large seed size in Japan, did not allow efficient improvement of productivity. We also recently found a substantial variation of yielding performance under an environment of Indonesia among divergent cultivars from tropical and temperate regions through in a part biomass productivity. Gas exchange activity again seems to be involved. Unlike in North America where transpiration adjustment is considered necessary to avoid terminal drought, under the monsoon climate with wet summer plants with higher activity of gas exchange than current level might be advantageous. In order to explore higher or better-adjusted canopy function, the methodological development is demanded for canopy-level evaluation of transpiration activity. The stagnation of soybean yield would be broken through controlling variable water environment and breeding efforts to improve the quality-oriented cultivars for stable and high yield.

• Korean Journal of Poultry Science
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• v.18 no.1
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• pp.43-55
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• 1991

This experiment was conducted to study the sparing effects of animal proteins on weight gain, nutrients utilizability and economic analysis of broiler. Experiment diet contained different ratio of animal and plant protein and were supplemented different levels of methionine and lysine for the respective protein levels. A total of 264 broiler chicks were fed four diets of control, $T_1$, $T_2$ and $T_3$ for 42 days from April 7, 1990 to May 19, 1990. Dietary protein levels of control, $T_1$, $T_2$ and $T_3$ for starter and finisher were 20~18, 18~16, 18~16 and 22~20%, respectively. Methionine and lysine levels of control, $T_1$, $T_2$ and $T_3$ were 0.4~1.1, 0.44~1.21, 0.48~1.32 and 0.48~1.32% for starter diet, respectively, and were 0.32~0.90. 0.35~0.99, 0.38~1.08 and 0.38~1.08% for finisher diet, respectively. The results obtained were summarized as follows. 1. The birds fed control diet gained most for overall period. $T_3$ treatment which was high in protein, methionine and lysine levels gained most for finisher period. 2 The birds fed control diet consumed most feed, and the birds fed T$_3$ diet consumed least feed. for overall period. Feed conversion during 1~4 weeks was better in $T_1$ (1.51) and $T_2$ (1.53) than in control (1.61) and $T_3$ (1.63) . During 4~6weeks, feed conversion was better in $T_3$ (1.37) and control(1.58) than T, (2.05) and T, (2.16) (P＜0.01) 3. Dry matter, crude fiber and NFE utilizability were increased for 1~4 weeks and decreased for 4~6 weeks as methionine and lysine levels increased and crude protein utilizability tended to be increased as protein levels increased. 4. Abdominal fat content was lowest in bird fed control diet and was high in birds fed low protein diet Carcass percentage was highest at control and the abdominal fat content was higher in bird fed lower protein diet than bird fed other protein diets(P＜0.05). 5. Feed cost per kg weight gain was lowest at $T_3$ which contained more soybean oil meal than other feeds and next was control. According to the results of this experiment, it was revealed that optimum protein, methionine and lysine levels for starter and finisher broiler diet were 20~18, 0.4~0.32 and 1.1~0.9%, respectively.

• Journal of Food Hygiene and Safety
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• v.31 no.3
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• pp.186-193
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• 2016

An analytical method was developed for the determination of oxathiapiprolin in agricultural commodities. Oxathiapiprolin is a new oomycide (fungicide of piperidinyl thiazole isoxazoline class) which controls downy mildew in cucurbits caused by Pseudoperonospora cubensis (oomycete plant pathogen). Agricultural commodities were extracted with acetonitrile and partitioned with dichloromethane to remove the interference, adjusting pH between 9 and 10 by 1 N sodium hydroxide. After purification by silica SPE cartridge to clean up the interference of organic compounds, they were finally quantified by HPLC-UVD (high performance liquid chromatograph ultraviolet detector) using a wavelength at 260 nm and confirmed by LC-MS (liquid chromatograph mass spectrometer) in electro-spray ionization positive ion mode. The standard calibration curve was linear with coefficients of determination ($r^2$) 1.00 over the calibration ranges (0.025-2.5 mg/L). Recoveries were ranged between 86.7 to 112.7%, with relative standard deviations less than 10% at three concentration levels (LOQ, 10LOQ, and 50LOQ) performing five replicates. The overall results were determined and estimated according to the CODEX guidelines (CAC/GL40). The proposed method for determination of oxathiapiprolin residues in agricultural commodities can be used as an official method.

• KSBB Journal
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• v.14 no.3
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• pp.328-336
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• 1999

A green microalga, Chlorella vulgaris UTX 259, was cultivated in a bench-scale raceway pond. During the culture, 15%(v/v) $CO_2$ was supplied and industrial wastewater discharged from a steel-making plant was used as a culture medium. In a small scale culture bottle, the microalga grew up to 1.8 g $dm^{-3}$ of cell concentration and ammonia was completely removed from the wastewater with an yield coefficient of 25.7 g dry cell weight $g^{-1}\;NH_3-N$. During the bottle-culture, microalga was dominant over heterotrophic microorganisms in the culture medium. Therefore, the amount of carbon dioxide fixation could be estimated from the change of dry cell weight. In a semi-continuous operation of raceway pond with intermittent lighting (12 h light and 12 h dark), increase of dilution rate resulted in increase of the ammonia removal rate as well as the $CO_2$ fixation rate but the ammonia removal efficiency decreased. Ammonia was not completely removed from the medium (wastewater) of raceway pond which was operated in a batch mode under a light intensity up to 20 klux. The incomplete removal of ammonia was believed due to insufficient light supply. A mathematical model, capable of predicting experimental data, was developed in order to simulate the performance of the raceway pond under the light intensity of sun during a bright daytime. Simulation results showed that the rates of $CO_2$ fixation and ammonia removal could be enhanced by increasing light intensity. According to the simulation, 80 mg $dm^{-3}$ of ammonia in the medium could be completely removed if the light intensity was over 60 klux with a continuous lighting. Under the optimal operating condition determined by the simulation, the rates of carbon dioxide fixation and ammonia removal in the outdoor operation of raceway pond were estimated as high as $24.7 g m^{-2} day^{-1}$ and $0.52 g NH_3-N m^{-2} day^{-1}$, respectively.