• Journal of Pharmacopuncture
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• v.4 no.1
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• pp.123-124
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• 2001

Purpose. Free radicals are implicated in the pathophysiology of aging, ischemic injury and neurodegenerative disorders. To deform]no whether Hominis Placenta extract prevents $H_2O_2$-induced apoptosis, we have performed morphological and biochemical analyses for the detection of apoptotic phenomena in the pineal tumor cell line $PGT-{\beta}$ We have also peformed cytochemical and immunocytochemical analyses for the detection of changes in nitric oxide synthase (NOS) activity and estimated the expression . of apoptotic genes using reverse transcription-polymerase chain reaction (RT-PCR) Methods. $PGT-{\beta}\;cells$ were pretreated with Hominis Placenta extracts $(0,\;10^{-2}\;{\mu}g/ml)$ for 2 hours and then exposed to $H_2O_2\;(0,\;50\;{\mu}M)$ for 3 hours. Appearance of apoptotic characteristics were monitored using 4, 6-diamidino-2-phenylindole dihydrochloride (DAPI) staining assay, terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) assay and flow cytometric analysis. NOS activity was measured by NADPH-diaphorase cytochemistry. Expression of inducible NOS (iNOS) and nuclear factor kappa B (NF k B) was assessed via immunocytochemistry. The expression of apoptotic genes was examined by RT-PCR. Results. After 3 flours of exposure to $H_2O_2$, it was shown that $PGT-{\beta}\;cells$ treated with $H_2O_2(50\;{\mu}M)$ exhibit classical apoptotic features and increases in NOS activity and caspase-3 expression. Treatment with Hominis Placenta extract resulted in a reduced occurrence of apoptotic features. DAPI staining, TUNEL and flow cytometric assays revealed decreases in the occurrence of nuclear fragmentation and in the sub-Gl fraction in the $PGT-{\beta}\;cells$ treated with Hominis Placenta extract. Cells treated with Hominis Placenta extract also showed lower activity of NADPH-diaphorase and immunoreactivities of both iNOS and NF k B than those of $H_2O_2$-treated cells which were not treated with Hominis Placenta extract. By RT-PCR, it was shown that the level of caspase-3 mRNA was derreased In the cells treated with Hominis Placenta . extract. Conclusions. This study shows that Hominis Placenta extract prevents $H_2O_2$-induced apoptosis in $PGT-{\beta}\;cells$; inhibitions of iNOS and caspnse-3 are possible mechanisms of the protection against apoptosis.

• Journal of Pharmacopuncture
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• v.13 no.4
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• pp.139-147
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• 2010

Objectives : The Purpose of this study is to investigate and report the effectiveness of Hominis Placenta using Pharmacopuncture treatment for trigger finger. Methods : 3 Patients are admitted at Dept. of Oriental Rehabilitation, Bu-Chun Jaseng Oriental Medicine Hospital, diagnosed as Trigger finger and treated with Hominis Placenta Pharmacopuncture. Each cases are measured and assessed by Quinnell's classification of triggering and VAS (Visual Analogue Scale) scores. Results : 3 Patients of trigger finger have a different kind of cause and fingers lesion they have, but nodules are not significantly found up, so we could classify all of 3 patients to diffuse type. After treatment of Hominis placenta Pharmacopuncture, spontaneous pain and tenderness, grades of triggering are decreased significantly. We would expect that Hominis placenta Pharmacopuncture has a effect on degenerative diseases of diffuse type's tendon sheath. Conclusions : Trigger finger is generally divided into two stages, inflammatory and degenerative stage, and when degenerative stage, Hominis placenta pharmacopuncture appears to be effective.

• Journal of Pharmacopuncture
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• v.18 no.2
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• pp.51-59
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• 2015

Objectives: This study was performed to analyze the toxicity and to find the lethal dose of the test substance Hominis placenta pharmacopuncture when used as a single-dose in 6 week old, male and female Sprague-Dawley (SD) rats. Methods: All experiments were conducted at Biotoxtech (Chungwon, Korea), an institution authorized to perform non clinical studies, under the regulations of Good Laboratory Practice (GLP). SD rats were chosen for the pilot study. Doses of Hominis placenta pharmacopuncture extracts, 0.125, 0.25 and 0.5 mL, were administered to the experimental group, and 0.5 mL doses of normal saline solution were administered to the control group. This study was conducted under the approval of the Institutional Animal Ethics Committee. Results: No deaths or abnormalities occurred in any of the groups. Also, no significant changes in body weights were observed among the groups, and no significant differences in hematology/biochemistry, necropsy, and histopathology results were noted. Hematologically, some changes in the male rats in two experimental groups were observed, but those changes had no clinical or toxicological meaning because they were not dose dependent. Histopathological tests on the injected parts showed cell infiltration in the male rats in one of the experimental groups; however, that result was due to spontaneous generation and had no toxicological meaning. Therefore, this study showed that Hominis placenta pharmacopuncture had no effect on the injected parts in terms of clinical signs, body weight, hematology, clinical chemistry, and necropsy. Conclusion: As a result of single-dose tests of the test substance Hominis placenta pharmacopuncture in 4 groups of rats, the lethal dose for both males and females exceeded 0.5 mL/animal. Therefore, the above findings suggest that treatment with Hominis placenta pharmacopuncture is relatively safe. Further studies on this subject are needed.

• Journal of Haehwa Medicine
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• v.25 no.1
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• pp.45-52
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• 2016

Objectives : The purpose of this study is to report the clinical effects of Hominis Placenta Pharmacopunture(HPP) on Vertigo. Methods : A 82-year-old female patient whose chief complaint was Vertigo was treated with Placenta Pharmacopunture at CV6 and GB21, twelves times for a month. Acupuncture and herbal medicine were added depending on the symptoms. Laboratory test was taken and Korean Version of Dizziness Handicap Inventory was examined before and after each treatment. Results : After the treatments, the symptoms were improved. Conclusions : Hominis Placenta Pharmacopuncture may be an efficacious therapy for Iron Deficiency Anemia.

• Journal of Pharmacopuncture
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• v.4 no.3
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• pp.119-126
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• 2001

Objective : In order to report the process of treatment of Aplastic anemia by herbal acupuncture Methods: This case was diagnosed as deficiency of Yang of spleen and kidneys, deficiency of Ki and blood, and fevescence from the deficiency of Urn. Hominis Placenta herbal acupuncture and Bee-venom acupuncture were choosen as the method to treat the case. Results: 1. The nocturnal fevescence and indigestion was decreased during the times when Hominis Placenta herbal acupuncture was used once a day. 2. The mobility of the joints was improved and the inflamation on the joints was decreased during the times when Bee-venom acupuncture was used every other day. 3. The interval between the transfusion was extended during the times when Hominis Placenta herbal acupuncture and Bee-venom acupuncture were used by turns. Conclution : Hominis Placenta herbal acupuncture and Bee-venom acupuncture sucessfully imporved the symtoms of patient with Aplastic anemia

• Journal of Pharmacopuncture
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• v.8 no.3
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• pp.99-105
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• 2005

Objectives : The purpose of this study is to analyze the functional and clinical improvement effect on temporomandibular disorder by additional Hominis Placenta and bee venom herbal acupuncture treatment. Methods : A patient was treated with Hominis Placenta, bee venom herbal acupuncture, simple acupuncture, chiropractic therapy, and herbal medicine. We evaluated the improvement by visual analogue scale(VAS) score, mouth opening range, and X-ray image. Results : The VAS score was significantly decreased and mouth opening range was increased after treatment. X-ray image showed improved change after treatments. Conclusions : We think Hominis Placenta and bee venom herbal acupuncture treatment mainly contributed to the improvement of temporomandibular disorders. Further study is needed for the confirmation of this effect of on temporomandibular disorders.

• Journal of Acupuncture Research
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• v.22 no.6
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• pp.85-92
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• 2005

Objectives : This study is to evaluate the efficacy of Hominis Placenta Herbal Acupuncture(HPA) on dysmenorrhea. Methods : Volunteers of fourteen subjects who used to feel pains in menstrual period were employed to answer the questionnaire. Subjects were divided into two groups Hominis Placenta Herbal Acupuncture(HPA) treatment group(Sample Group) and Normal Saline(N/S) treatment group(Control Group). HPA and N/S were injected on the Cheonchu(ST25), Gwanwon(CV4), Joksamni(ST36) and Hyeolhae(SP10) acupuncture points. Subjects were treated depending on menstrual period, two times a weeks, in total four times after ovulation cycle. Digital Infrared Thermal Imaging(D.I.T.I.) was taken and Visual Analog Scale(VAS) was examined before and after each treatment. Results : The VAS score of Sample Group were decreased significantly compared to that of Control Group.(p<0.05) The changes of difference of abdominal mean temperature of Control Group and Sample Group were significancy. HPA therapy reduced difference of right and left abdomial thermal temperature more than N/S therapy in dysmenorrheic patients. Conclusion : The Hominis Placenta Herbal Acupuncture therapy may be good effects on the Dysmenorrhea.

• The Journal of Korean Obstetrics and Gynecology
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• v.23 no.3
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• pp.139-155
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• 2010

Purpose: The aim of this study was to investigate the effect of hominis placenta herbal aupuncture therapy on the postpartum women's heat feeling, sweat and thirst. Methods: The subjects were 25 women who admitted for postpartum treatment in Hospital of Woosuk University from 15th November 2008 to 5th February 2010. The control group(normal saline group, NSG) were 12 women and hominis placenta herbal acupunture treatment group(HPAG) were 13 women. We injected at Kwanwon(CV4), Sinsu(BL23). We checked VAS(heat feeling, sweat and thirst), vital sign, wrong response. We tested CBC, 7-zone-diagnostic system, HRV before and after clinical study. Results: VAS of thirst was significantly decresed in the HPAG than NSG. Ln(LF) of HPAG was lower than NSG after clinical study. Conclusion: The results suggest that hominis placenta herbal aupuncture therapy may be useful for the postpartum women.

• Journal of Acupuncture Research
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• v.24 no.6
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• pp.75-88
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• 2007

Objectives : We investigated the effects of Hominis placenta Herbal acupuncture on rheumatoid arthritis. Methods : We investigated the Type II collagen-induced arthritis test, vascular permeability test, phenyl benzoquinone-induced writhing test, hot plate test, carbon clearance test, determination of prostaglandin $E_2$ content and cyclooxygenase-2 activity test. Results : Hominis placenta Herbal acupuncture suppressed Type II collagen-induced arthritis in DBA/1J mice and vascular permeability with 10.2% inhibition in ICR mice. We see the phenyl benzoquinone-induced writhing test and the hot plate test in the analgesic activity test. In the phenyl benzoquinone-induced writhing test, Hominis placenta Herbal acupuncture showed that it increased its analgesic effect more than the control(test?) by 85.5%. In the hot plate test, it was also shown that the analgesic effect increased more than the control(test) by 32.1%. In the carbon clearance test, the immuno-stimulatory effect showed more than the control(test) by 8.0%. The formation of prostaglandin $E_2$ was also reduced more than the control(test) by 10.1%. Finally, cyclooxygenase-2 activity was inhibitedwith 36.3%. Conclusions : Hominis placenta Herbal acupuncture may be useful for the treatment of rheumatoid arthritis after injection.

• Journal of Acupuncture Research
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• v.28 no.5
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• pp.135-142
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• 2011

Objectives : This study was to investigate on the hominis placenta pharmacopuncture of FBSS patient who were diagnosed as the cauda equina syndrome which has been described as a complex of low back pain, bilateral sciatica, saddle anesthesia and hypoesthesia in the lower extremity with bladder and bowel incontinence. Methods : The patient was treated with hominis placenta parmacopuncture at Samchosu($BL_{22}$), Shinsu($BL_{23}$), Sangryo($BL_{31}$), Charyo($BL_{32}$), Jungryo($BL_{33}$) and Haryo($BL_{34}$) with oriental medical conservative treatment. We estimated by visual analog scale and Oswestry disability index and nerve level dermatome test for evaluate the effect of Hominis Placenta Pharmacopuncture with oriental conservative treatment. Results : After treatment, patient's visual analogue scale score, Oswestry disability index score, bilateral sciatica, saddle anesthesia and hypoesthesia in the lower extremity with bladder and bowel incontinence were generally decreased. Conclusions : The hominis placenta pharmacopuncture with oriental medical conservative treatment might be an effective method to treat the FBSS patient who were diagnosed as the cauda equina syndrome.