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Nutrient Intake, Its Utilization, Rumen Fermentation Pattern and Blood Bio-Chemical Constituents of Sheep Fed Urea Treated Mustard (Brassica campestris) Straw

  • Misra, A.K.;Karim, S.A.;Verma, D.L.;Mishra, A.S.;Tripathi, M.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.12
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    • pp.1674-1680
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    • 2000
  • A study was conducted to compare the feeding value of urea treated and untreated mustard straw (MS) for sheep. Treated MS was prepared by adding urea-N at 1.84% and followed by packing in a pit silo for 21 days. Two groups of six empty Avikaline ewes were fed untreated (UTMS) and treated (TMS) mustard straw along with 200 g concentrate per head daily for 90 days. Untreated MS had 0.41% N and the urea treatment increased its N value to 1.58 %. The cell wall constituents were decreased in the TMS except for cellulose which remained unaffected. Dry matter intake of TMS was consistently higher than that of UTMS. Digestibility of DM, OM and fibre fractions of MS improved by the urea treatment. Ewes in both groups were in positive N balance while % N retention was lower in UTMS (26.30%) than in TMS (52.14%). The TMS fed group on average consumed 30.2 g DM, 2.9 g digestible crude protein and $0.2MJ\;DE\;per\;kg\;BW\;day^{-1}$ and maintained their weight whereas, the UTMS fed ewes lost weight. The VFA concentration in rumen liquor was higher in TMS than in UTMS. Total-N, ammonia-N and TCA-precipitable-N were also higher in TMS fed ewes. Blood glucose concentrations in the two groups were similar at initiation of the study. However the glucose concentration of UTMS fed group was significantly (p<0.01) lower than those fed UTMS at the termination of the study. Urea-N concentration was also higher in TMS fed group after 90 days of feeding period. It is concluded that urea treatment of MS improved N value of MS from 0.41% to 1.58% along with sizable improvement in nutritive value and in conjunction with 200 g concentrate, TMS can serve as maintenance ration for sheep. ($ME_{lakt}/ME_{m}=1.46$).

Penetration and Microleakage Assessment of Flowable Resin Applied on Carious Fissure Following Various Fissurotomy Techniques (교합면 우식열구에서 열구성형술 방법에 따른 유동성 레진의 침투도와 미세누출 평가)

  • Kwon, Seok;Lee, Sangho;Lee, Nanyoung;Jih, Meongkwan;Yoon, Youngmi
    • Journal of the korean academy of Pediatric Dentistry
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    • v.45 no.1
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    • pp.90-97
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    • 2018
  • The purpose of this study was to evaluate the effect of fissurotomy on the penetration and microleakage of flowable resins for carious fissures. A total of 250 extracted premolars with early fissure caries were selected and divided into five groups according to the fissurotomy; no fissurotomy (n = 50), fissurotomy with $Fissurotomy^{(R)}$ original bur (n = 50), fissurotomy with $Fissurotomy^{(R)}$ Miro NTF bur (n = 50), fissurotomy with SF104R tapered diamond bur (n = 50), fissurotomy with 1/2 round carbide bur (n = 50). Two types of flowable resins ($UniFil^{(R)}Flow$, $Filtek^{(R)}Flow$) were used as sealing materials. All samples were sectioned and observed using a stereoscopic microscope after thermocycling and immersing in methylene blue solution. The adaptation of flowable resin to the fissure wall was observed using scanning electron microscopy. The penetration of flowable resin into the carious fissure was significantly increased by fissurotomy, which also decreased microleakage. Fissure preparation using different burs showed a significantly different in penetration, but did not show any difference in microleakage. $Unifil^{(R)}Flow$ showed better penetration than $Filtek^{(R)}Flow$, but there was no significant difference in microleakage. Fissurotomy can be used to increase the penetration of flowable resin into carious fissures and decrease microleakage.

Tegumental Ultrastructures of Spirometra erinacei by Developmental stages (만손열두조충의 발육단계별 표피 미세구조)

  • Sohn, Woon-Mok;Lee, Jin-Ha
    • Applied Microscopy
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    • v.35 no.1
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    • pp.41-56
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    • 2005
  • Present study was performed to observe the tegumental ultrastructures by the developmental stages which derived from the experimental life cycle of Spirometra erinacei in laboratory conditions. In SEM view, coracidium was spherical in shape with numerous cilia, and its surface was covered with long cilia, tuberclelike projections with millet-like processes, and small holes. The body surface of procercoid was covered with numerous pointed microtriches except that of frontal pit with stout spine-like ones. However that of cercomer was covered with somewhat sparse blunt-tiped microtriches. Plerocercoids of 3 days old resembled the mature procercoid in shape, and their frontal pits were covered with numerous stout spine-like microtriches. However frontal pit and body surface in more than 5 days old ones were covered with conoid microtriches. On the surface of adult scolex, hairly long filamentous and stout short microtriches were mixedly distributed. Filamentous microtriches were more densely distributed in the anterior portion than in the posterior of scolex. The neck and immature proglottid were covered with only stout short conoid microtriches. In TEM view of coracidia, embryophore and oncosphere were obviously distinguished. The embryophore contained numerous glycogen particles, mitochondria and lipid granules. The cilia on the surface of embryophore rooted in the coracidial sheath, and consisted of 9 pairs of microtubules and 2 core complex. The oncosphere was covered with a thin and unarmed tegument, and was multi-nucleated. The protoplasmic layer of procercoid and plerocercoid consisted of disc-shaped bodies, vacuoles and mitochondria. Their tegumental cells commonly retained a nucleus, granular endoplasmic reticulums and secretory granules. The protoplasmic layer of plerocercoid was more compacted than that of procercoid. From the above results, it was confirmed that the tegumental ultrastructures are something different according to the developmental stages of S. erinacei.

Development of 2.5D Electron Dose Calculation Algorithm (2.5D 전자선 선량계산 알고리즘 개발)

  • 조병철;고영은;오도훈;배훈식
    • Progress in Medical Physics
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    • v.10 no.3
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    • pp.133-140
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    • 1999
  • In this paper, as a preliminary study for developing a full 3D electron dose calculation algorithm, We developed 2.5D electron dose calculation algorithm by extending 2D pencil-beam model to consider three dimensional geometry such as air-gap and obliquity appropriately. The dose calculation algorithm was implemented using the IDL5.2(Research Systems Inc., USA), For calculation of the Hogstrom's pencil-beam algorithm, the measured data of the central-axis depth-dose for 12 MeV(Siemens M6740) and the linear stopping power and the linear scattering power of water and air from ICRU report 35 was used. To evaluate the accuracy of the implemented program, we compared the calculated dose distribution with the film measurements in the three situations; the normal incident beam, the 45$^{\circ}$ oblique incident beam, and the beam incident on the pit-shaped phantom. As results, about 120 seconds had been required on the PC (Pentium III 450MHz) to calculate dose distribution of a single beam. It needs some optimizing methods to speed up the dose calculation. For the accuracy of dose calculation, in the case of the normal incident beam of the regular and irregular shaped field, at the rapid dose gradient region of penumbra, the errors were within $\pm$3 mm and the dose profiles were agreed within 5%. However, the discrepancy between the calculation and the measurement were about 10% for the oblique incident beam and the beam incident on the pit-shaped phantom. In conclusions, we expended 2D pencil-beam algorithm to take into account the three dimensional geometry of the patient. And also, as well as the dose calculation of irregular field, the irregular shaped body contour and the air-gap could be considered appropriately in the implemented program. In the near future, the more accurate algorithm will be implemented considering inhomogeneity correction using CT, and at that time, the program can be used as a tool for educational and research purpose. This study was supported by a grant (#HMP-98-G-1-016) of the HAN(Highly Advanced National) Project, Ministry of Health & Welfare, R.O.K.

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The Correlation between HRCT Emphysema Score and Exercise Pulmonary Testing Parameters (HRCT Emphysema Scoring과 운동부하 폐기능검사 지표들 간의 상관관계)

  • Choi, Eun-Kyoung;Choi, Young-Hee;Kim, Doh-Hyung;Kim, Yong-Ho;Yoon, Se-Young;Park, Jae-Seuk;Kim, Keun-Youl;Lee, Kye-Young
    • Tuberculosis and Respiratory Diseases
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    • v.50 no.4
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    • pp.415-425
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    • 2001
  • Background : The correlation between the high resolution computed tomography(HRCT) emphysema score and the physiologic parameters including resting and exercise pulmonary function test was investigated in 14 patients($60.6{\pm}10.3$ years) with pulmonary emphysema. Methods : The patients underwent a HRCT, a resting pulmonary function test, and incremental exercise testing(cycle ergometer, 10 W/min). Computed tomography scans were obtained on a GE highlight at 10 mm intervals using 10 mm collimation, from the apex to the base after a full inspiration. The emphysema scores were determined by a CT program 'Density mask' outlining the areas with attenuation values less than -900 HU, indicating the emphysema areas, and providing an overall percentage of lung involvement by emphysema. Results : Among the resting PFT parameters, only the diffusing capacity(r=-0.75) and $PaO_2$ (r=-0.66) correlated with the emphysema score(p<0.05). Among the exercise test parameters, the emphysema score correlated significantly with the maximum power(r=-0.74), maximum oxygen consumption(r=-0.68), anaerobic threshold(V-slope method: r=-0.69), maximal $O_2$-pulse(r=-0.73), and the physiologic dead space ratio at the maximum workload(r=-0.80)(p<0.01). Conclusion: We could find that exercise testing parameters showed a much better correlation with the HRCT emphysema score, which is known to have a good correlation with the pathologic severity than the resting PIT parameters. Therefore it is suggested that exercise testing is superior to resting PIT for estimating in the estimation of the physiologic disturbance in emphysema patients.

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Dental Caries Status of 14-16 Year Old Adolescents in Yangsan Area (양산시 거주 14-16세 청소년의 치아우식증 상태에 대한 조사연구)

  • Kwon, Bomin;Bae, Ikhyun;Kim, Shin;Kim, Jiyeon;Jeong, Taesung
    • Journal of the korean academy of Pediatric Dentistry
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    • v.41 no.1
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    • pp.8-17
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    • 2014
  • Although adolescents usually show peculiar aspects in oral health status, there have been not so many studies about it, while there have been innumerable studies for children. This study aimed at evaluating the prevalence of dental caries of adolescents in Yangsan in Republic of Korea, detecting its contributing factors, and distinguishing the high-risk group by epidemiological mass survey. Besides, the significance including early caries lesion in diagnosis criteria was assessed with minimal invasive concept. This survey was performed on 1,371 adolescents aged 14 to 16 in Yangsan as subject examinees by a single trained examiner. The dental caries status was checked by oral examination at schools and questionnaire survey was accompanied for some relevant risk factors. The data were analyzed to yield the results as follows: Regarding the caries experience, the DMFT score by WHO criteria with ECL including or not was 4.79 and 3.97 respectively. In both the criteria, female showed higher values and that of high-risk group was almost twice compared with whole examinees by Sic Index. About pit & fissure sealed rate, it revealed the tendency that the more sealed teeth, the lower DMFT values. Among the studied risk factors, only the habit of brushing before going to bed showed positive relationship (p < 0.05).

Sampling Methods for the 'dark grey cutworm' (Agrotis tokionis B.) Larval Population and Effect of its Larval Density on Tobacco Yield (숯검은밤나방(Agrotis tokionis B.) 유충개체군의 밀도추정방법 및 유충밀도와 연초감수량의 관계)

  • Kim S.S.;Boo K.S.;Kang Y.K.
    • Korean journal of applied entomology
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    • v.20 no.4 s.49
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    • pp.217-222
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    • 1981
  • As a primary study on the economic injury level of A. tokionis larval populations in tobacco fields, we carried out some experiments for the evaluation of sampling efficiency of 3 sampling methods (pit-fall traps of apple pomace and rice bran, and clover patches) for the larval population and the regressions between loss and infested larval density The $10\times10cm$ clover patch showed a better sampling efficiency $11.8\;to\;18.0\%$ than the others. The sampling efficiency of clover patch becomes higher when the plots did not have any green plants. The linear regression equation (Y=4.2+1.383x) between loss (Y:kg/10a) and infested larval density (X: no. of larvae/plot) which was obtained by substitution of damage ratio and corrected damage ratio fitted the observed data better than the one (Y=2.68X) obtained without substitioning.

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Measurements of Greenhouse Gas from the Manure in the Piggery (축산 돈사에서 온실가스 측정 방법에 대한 연구)

  • Kam, D.H.;Park, K.H.;Choi, D.Y.;Jung, M.S.;Min, B.R.;Lee, D.W.;Kim, J.K.
    • Journal of Animal Environmental Science
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    • v.17 no.3
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    • pp.155-162
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    • 2011
  • This study was conducted to suggest the measurement procedure and to build up national greenhouse gas inventory database of animal agricultural sector by assessing methane and nitrous oxide emissions according to IPCC guidelines for national greenhouse gas inventory report in order to correspond to the Climate Change Convention. Ten house-made steady-state Half dome floating chambers were used to collect air samples emitted from slurry stored in the pit under the slat. Those chambers were spread out in order that air samples might represent the whole area of slurry under the slat. Fresh air was pumped into the chambers by $5{\sim}9{\ell}/min$ and air inside the chambers was sampled by $1{\ell}/min$. Surplus air by the higher flow rate of fresh air than sampling flow rate was passed through a hole on the top of chambers. Nitrous oxide fluxes measured from 10 locations would be negligible as concentrations between background air and sampled air from the chambers were within the error range. However, mean $CH_4$ fluxes were $0.15{\sim}1.02mg/m^2{\cdot}s$. The application of continuous greenhouse gas measurement techniques would be preferred if the patterns of greenhouse gas emissions are considered.

Effects of dye-guidance brushing etching technique on the performance of pits and fissures sealant (Dye-guidance와 brushing을 통한 산부식 방법이 치면열구전색술의 수복의 질에 미치는 영향)

  • Hung, Phan Ai;Lee, Nan-Young;Lee, Sang-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.34 no.1
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    • pp.106-121
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    • 2007
  • The purpose of this study was to examine the effects of suggested etching method on the performance of pits and fissures sealant. In the first part, seventy extracted sound human permanent third molars and premolars were used. The teeth were randomly divided and performed in three different groups as follows : conventional etching, enameloplasty, and testing group. Non-pumicing, dye-guidance vigorous brushing-start etching technique was applied on the occlusal of testing group. Then the pit and fissure sealant was applied on all of the specimens. After the thermocycling and immersing in 1% methylene blue, the resin embedded sections were made. The microleakage data on the section were then recorded under the stereoscope and statistic analysis was done. Additional experiments were also performed : direct fissure surface etched pattern experiment, replica study, and micro-shear bond strength testing observation. The second part included two groups. A paired study was designed to evaluate the influence the environment has on the performance of the sealant. After etching, half of each occlusal surface received one of the two following treatments in succession: sealing in laboratory and intraoral condition (group 1), sealing in intraoral condition with and without a single-bonding agent (group 2). The results of present study can be summarized as follows: - The microleakage of testing group was significant different with conventional method (P<.05) and was not different with the enameloplasty group (P>.05). - The quality and quantity of etched enamel were improved. - Microshear bond strength of testing group was higher than control group (p<.05). - The environmental condition was influenced on the performance of the sealant. The testing etching method modified the capacity of the etching agent to penetrate into the pits and fissures, and simultaneous enhance their efficiency in vitro condition.

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A Study of Columnar Joint in Goheung, Jeollanam-do, Korea (전라남도 고흥지역에 분포하는 주상절리에 관한 연구)

  • Son, Jeong-Mo;Ahn, Kun Sang
    • Journal of the Korean earth science society
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    • v.37 no.6
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    • pp.332-345
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    • 2016
  • The columnar joints in Goheung are developed in three places of Yuju-san area, Palyeong-san and Yongbawi area. Vertical and fan-shaped columnar joints which have maximum width 100 m and maximum heigh 50 m are developed in the Yuju-san area Columnar joints are developed next to the road near the the Yuju-san and along the coast of Jijuk-do. Thick columnar joints of maximum width 1m are developed in the Paryeong-san area. Horizontal columnar joints of maximum width 50 cm at length of polygon side are developed on dyke in the Yongbawi area. The columnar joints show high rate of rectangles and pentagons in the number of polygons. The length of polygon side of columnar joints in study area ranges from 10 to 100 cm, and 20 cm among the range appears in high frequency. Columnar joints are developed vertically to the ground from the cooling surface in Yuju-san and Palyeong-san area. Columnar joints in Yongbawi area are developed vertically to the contact of country rocks. As a result, the columnar joints began cooling from the country rock contact. And columnar joints are developed vertically to contact surface. The rocks in columnar joints is rhyolitic welded tuff in Yuju-san and Palyeong-san area, dacite in Yongbawi area. In the acid volcanic rocks flow structure well developed. The white phenocryst mineral about 2 mm size by eye, is usually feldspar, and includes some quartz. The rate of $SiO_2$ is 70wt.% or more. It is the last stage of differentiation to calc-alkaline series. The columnar joints of the Yuju-san area are expected to be distributed along a band that extends to about 1km east of the stone pit.