• Journal of Korean Society of Forest Science
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• v.78 no.4
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• pp.401-411
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• 1989

The embryos of Pinus taeda, P. rigida, and P. taeda ${\times}$ rigida were cultured for adventitious shoot regeneration in vitro. Culture media were modified from Gresshoff and Doy (MGD), Murashige and Skoog (MMS), Lloyd and McCown (MLM), and Schenk and Hildebrandt (MSH). NAA was added to initiation media at a concentration of 0.1 or 0.01 mg/l. BAP was used at the concentrations of 0.1. 0.5, 1, 2, or 5mg/l. Each explant was induced for 3-4 weeks on solid medium. All explants were cultured up to 16 weeks. Illumination was about $1506{\pm}540lux$ at the level of the tissues in the growth room with a temperature of $25{\pm}2^{\circ}C$. A 16-hour photoperiod per 24 hours was used. Half-strength medium was used for all the subcultures. For shoot production by loblolly pine, MMS, MLM, or MSH is preferred with 5 mg/l BAP with either 0.1 or 0.01 mg/l NAA. For shoot production by pitch pine, MMS, MLM, or MSH is recommended with 2 or 5 mg/l BAP with 0.1 mg/l NAA. For shoot production by the hybrid pine, MMS or MLM is more effective with 1, 2 or 5 mg/l BAP with 0.1 mg/l NAA. There were no differences recognized among the species tried in the patterns of bud formation and shoot development. Different composition of media, in major and minor salts or possibly in vitamins, should be tested for the two developmental stages of adventitious shoots ; the induction of shoot buds and the elongation of them into shoots.

• Journal of Korean Society of Forest Science
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• v.59 no.1
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• pp.31-36
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• 1983

Flowering time of Pinus rigida Mill and Pinus taeda L, in an $F_1$-hybrid seed orchard was investigated for five years from 1971 through 1975. The two tree species flowered during late April to early May at the observation site, Flowering patterns of the two species of different sex of the same species changed yearly during the five observation years. Floral development of the two species appeared to depend largely on temperature factor during the period of the initiation of floral organs up to flowering provided that other environmental factors are normal. Six-days difference in flowing time between female inflorescence of pitch (flower later) and male inflorescence of loblolly (flower earlier) pines effectively isolated the two species reproductively on population levels. Not all of selected trees of the two species for their synchronized flowering appeared to be useful as parental trees for the establishment of $F_1$-hybrid seed orchards. With the result from this investigation the author suggested to use a modified simple recurrent selection method for pitch-loblolly hybrid pine breeding.

• Journal of Korean Society of Forest Science
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• v.112 no.2
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• pp.258-266
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• 2023

Pine wilt disease is caused by the pine wood nematode (Bursaphelenchus xylophilus) and has killed many pine trees in Europe and Northeast Asia, including South Korea. Resistance to pine wilt disease varies among species. Previous studies were mostly conducted in nature or greenhouses and only a few in test fields. In this study, seedlings of six pine species (Pinus thunbergii, P. koraiensis, P. densiflora, P. parviflora, P. rigida × P. taeda, and P. strobus) were artificially inoculated by pine wood nematodes in the test field. The Wilt Index was measured every 2 weeks after inoculation in addition to the mortality rate, detection rate, and pine wood nematode concentration measurement after 24 weeks. The pine wilt disease mortality rates were P. thunbergii (80%), P. koraiensis (77.8%), P. densiflora (62.5%), and P. parviflora (22.0%), and both P. rigida × P. taeda and P. strobus survived. The pine nematode detection rates were the same among the species except for P. rigida × P. taeda pine (22.2%). High Wilt-Index values were obtained for P. thunbergii, P. koraiensis, and P. densiflora, which had mortality rates higher than the other species. Furthermore, there were no significant differences in the Wilt Indexes between P. parviflora, P. rigida × P. taeda, P. strobus, and the control group. Statistically, P. thunbergii and P. koraiensis showed high susceptibility to pine wilt disease, P. densiflora and P. parviflora showed moderate susceptibility, and P. rigida × P. taeda and P. strobus showed apparent resistance. These results provide basic data for pine wood nematode resistance breeding or as evidence of the need for afforestation of P. rigida × P. taeda and P. strobus.

• Journal of Korean Society of Forest Science
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• v.43 no.1
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• pp.39-50
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• 1979

Twelve Pinus taeda were used as mother trees and one P. rigida plus tree was used as pollen tree for 12 cross combinations. Nine P. densiflora plus trees(4 mother and 7 pollen trees) were used as parents for 11 cross combinations. Those parents and 10 progenies were analyzed for LAP of ${\times}$P. taeda rigida hybrid and P. densiflora and for peroxidase of ${\times}$P. taeda rigida. The analysis, based on the banding patterns, indicate three alleles for LAP-A locus(A1, A2, A3) and two alleles for LAP-B locus (B1, B2) in ${\times}$P. taeda rigida hybrids. Chi-square test on the segregation for progenies did not show significant differences. The results indicated good agreement with monohybrid Mendelian inheritance. Independence test for occurrence frequency of 2 alleles(LAP-A3, LAP-B2) illustrated that there is neither linkage nor repulsion relationship between LAP-A3 and LAP-B2 alleles. Three band at LAP-A locus were always exhibited from all parents and their progenies of P. densiflora. However, the occurrence of two bands at LAP-B locus was variable, one bands assumed as homozygous alleles(B2/B2) and two bands as heterozygous alleles(B1/B2). The segregation ratio for progenies of P. densiflora suggested that LAP-B locus may be controlled by two alleles(B1 and B2). Three Peroxidase loci(Px-A, Px-B, Px-C) assumed to be controlled by allozyme in ${\times}$P. taeda rigida hybrid. The Px-B and Px-C loci could not find out the variations from banding patterns of parents and their progenies, while the Px-A locus showed the variations of occurrence frequency by two bands. The segregation ratio for A1/A2 at LAP-A locus suggest that the peroxidase allozymes of ${\times}$P. taeda rigida hybrid appeare to be monomeric products; that is, Px-A locus may be controlled by two alleles (A1 and A2).

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.596-597
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• 2005

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.332-332
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• 2005

• Journal of Forest and Environmental Science
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• v.24 no.3
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• pp.119-126
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• 2008

Growth and yield prediction methods, ranging from whole-stand models to individual-tree models, have been developed for forest types managed for wood production. The resultant models are used for a host of purposes including inventory updating, management planning, evaluation of silvicultural alternatives, and harvest scheduling. Because of the large investment in developing growth and yield models for improved genotypes and silvicultural practices for loblolly pine (Pinus taeda) in the Southern United States, this region serves to illustrate approaches for modelling intensively managed forests. Analytical methods and computing power generally do not restrict development of reliable growth and yield models. However, long-term empirical observations on stand development, which are time consuming and expensive to obtain, often limit modelling efforts. Given that growth and yield models are used to project present volumes and to evaluate alternative treatment effects, data of both the inventory type and the experimental type are needed. Data for developing stand simulators for loblolly pine plantations have been obtained from a combination of permanent plots in operational forest stands and silvicultural experiments; these data collection efforts are described and summarized. Modelling is essential for integrating and synthesizing diverse information, identifying knowledge gaps, and making informed decisions. The questions being posed today are more complex than in the past, thus further accentuating the need for comprehensive models for stand development.

• The Korean Journal of Ecology
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• v.24 no.4
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• pp.213-217
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• 2001

The purpose of this study is to investigate the effects of hardwood competitions in stand structure and dynamics by applying prediction models for unthinned loblolly pine (Pinus taeda L.) plantations. A parameter recovery procedure for the Weibull distribution function based on four percentile equations was applied to develop diameter distribution prediction models. Four percentiles of the cumulative diameter distribution prediction equations were predicted as a function of quadratic mean diameter plus competin hardwood trees perhectare varibales. According to the results of this study. it was found that as the amount of competing hardwood trees increased, diameter distributions in terms of stand structure dynamics tended to be more skewed to the right. Therefore, the influence of non-planted hardwood trees interspecific competitoin on planted loblolly pines showed negative effects on the stand structure and dynamics.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.153-158
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• 2023

Pinus species are native to the Northern Hemisphere and some parts of the tropics to temperate regions in the Southern Hemisphere. They were used as food and medicine in prehistoric times. Massonianoside B is a compound found in pine trees and possesses antioxidant activity. In order to determine the presence and content of this compound in Pinus species, three different parts (needles, branches, and bark) of three Pinus species were extracted and investigated. High-performance liquid chromatography with a gradient elution system along with a reverse-phase INNO column with photodiode array detector was employed. Results showed that the branches of the three Pinus species had higher massonianoside B content (5.502 to 9.751 mg/g DW) than either the needles or bark. Furthermore, among the three species, P. rigida × P. taeda had the highest concentration of total massonianoside B (11.557 mg/g DW). These findings thus provide evidence of biological activity in Pinus species and establish a foundation for further research.

• Korean journal of applied entomology
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• v.31 no.4
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• pp.386-395
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• 1992

The black pine bast scale, Matsucoccus thunbergianae, causes severe damage to the black pines of southern coastal areas. It has one generation a year, coming out of the pine bark to mate a and lay eggs from early March to early May. Especially, its peak time is from late March to m mid-April. The host plants were found to be 7 species, Pinus thunbergii, P. strobus, P. taeda, P. b banksiana, P. massoniana, P. taiwannesis, and P. densiflora. The percentage of damaged black p pine by the age were 0.8% for I-year old ones, 3.7% for 4-6 years, 5.2% for 7-9 years, 9.3% f for 10-12 years, 8.1% for 13-15 years, 7.8% for 16-18 years, 6.7% for 19-21 years, 3.3% for 2 22-24 years, 1.9% for 25-27 years, and 1.1 % for 28 years. The highest rate of damage happened to 7 -20 years old trees, whereas the highest rate of damage upon branches happened to 6 6-7 years old ones. Finally, as far as the trunk is concerned, the damages proceeded from the m middle parts of the trunk, whose branches were alive up to the top. The rates of damage s spread in pure forest/mixed forest area were turned out to be 81.3/52.5% in Koheung, 80.3/ 5 58.1 % in Haenam, and 76.3/48.5% in Muan. That is, the damage rate was higher in the pure f forest areas than the mixed forest ones. The higher the density of trees beyond 20 trees per m 100$m^2$, the higher the damage rate was.