• Food Science and Biotechnology
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• v.15 no.3
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• pp.424-430
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• 2006

Low molecular weight polyphenols were isolated from hot water extracts of radiata pine (Pinus radiata) bark using a Sephadex LH-20 column and characterized by $^1H$ and $^{13}C$ NMR, UV, FT-IR, and GC-MS analyses. Major compounds isolated and identified were protocatechuic acid, trans-taxifolin, and quercetin. Trans-taxifolin, an important intermediate in biosynthetic route of proanthocyanidin (PA), was isolated in large quantities and indicates that PA is a major component of radiata pine bark. Small amounts of polyphenols were identified by GC-MS analysis. The presence of p-hydroxybenzoic acid, vanillic acid, protocatechuic acid, cis- and trans-feruic acid, p-coumaric acid, trans-caffeic acid, (-)-epicatechin, (+)-catechin, trans- and cis-taxifolin, (+)-gallocatechin, and quercetin was confirmed by comparison of mass fragmentation patterns and retention times (RT) with authentic samples. In addition, the presence of astringenin, astringenin glycoside, trans- and cis-leucodelphinidin was strongly assumed from characteristic mass fragment ions due to their conjugated structure and retro Diels-Alder reaction, and also from biosynthetic route of PA. GC-MS analysis allowed us to detect small amounts of phenolic acids and flavonoids and eventually discriminate trans- and cis-configuration in the identified polyphenols.

• Journal of agriculture & life science
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• v.43 no.2
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• pp.17-21
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• 2009

This experiment was conducted to clarify the effect of waste tire chip on the growth and nutrient content of Cymbidium Pine Clash 'Moon Venus'. There were no significant differences between bark only medium and mixed medium in leaf and stem growth. But in both medium and large size chip only, the leaf and stem growth decreased remarkably. The total number of roots, new roots and root length had similar tendency as in leaf and stem growth. In medium and large tire chip only, the decayed roots increased. There were no significant differences between bark only medium and mixed medium in total sugar, starch, content of chrolophyll, N, P and K, but decreased significantly in both medium and large size chip only.

• Journal of the Korean Wood Science and Technology
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• v.46 no.4
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• pp.323-337
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• 2018

Merkusii pine wood (Pinus merkusii) was extensively planted throughout Indonesia, where it is only indigenous in northern Sumatera, by the Dutch during colonial times. The demand for this wood species, especially in the domestic market, has increased notably, despite its limited durability regarding decay resistance. The purpose of this study was to investigate the occurrence of juvenile and mature wood on merkusii pine and to analyze its radial features from pith to bark based on density, shrinkage, static bending in modulus of rupture and modulus of elasticity, fiber length, microfibril angle, and durability. A segmented modeling approach was used to find the juvenile-mature transition. The graveyard test was performed to characterize the termite resistance from pith to bark of merkusii pine. The maturations were estimated to start at radial increments of 15 cm from the pith by fiber length and of 12 cm from the pith by microfibril angle. The projected figures for the proportion of juvenile wood at breast height were around 65%. The results also indicate that the pine wood was $0.52g/cm^3$ in density, 1.45 in coefficient of anisotropy, which indicates its good stability, 7597 MPa in modulus of elasticity, and 63 MPa in modulus of rupture. Natural durability against subterranean termite of the merkusii pine wood was rated to be grade 4 to 0 from pith to bark. However, after being treated by Entiblu and Enborer preservatives, its rating increased to grade 10 to 9.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.32 no.4
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• pp.66-73
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• 2000

Alkaline sulfite-anthraquinone(AS-AQ) cooking process has been applied to unground pine bark. The properties of the spent liquor such as molecular weight distributions, sulfonic equivalent weights, degree of sulfonation, phenolic hydroxyl groups and kaolin dispersing ability have been investigated to evaluate the possibility of using this liquor as concrete additives or binders. In the case of ground bark meal, more than 90% delignification was achieved at the optimal cooking conditions. However, applying these conditions on unground bark the delignification was slightly retarded. The decrease in the degree of delignification may be attributed to less penetration and diffusion of chemicals during the cooking of the bark. Increasing the cooking temperature only by $5^{\circ}C$ improved the delignification of the bark and about 90% delignification can be achieved. These results indicate that bark can be used successfully during AS-AQ cooking without any mechanical or physical pretreatment. The properties of lignin or polyphenol sulfonates in the spent liquor after AS-AQ cooking of the bark were compared with Sunflo-R, which is commercial lignosulfonate(CLS) prepared from wood. The weight average molecular weights(Mws) estimated by gel-filteration chromatography was found to be ranging from 1,200~1,800. These are considerably lower than those in CLS. Lignin or polyphenol sulfonates in the spent liquor of bark and CLS have similar degree of sulfonation, but the phenolic hydroxyl group was 1.8 times higher than CLS. Moreover, the dispersing abilities of the spent liquors were better than that of CLS, especially after 0.1% addition to kaolin suspension.

• Korean Journal of Agricultural Science
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• v.46 no.4
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• pp.781-790
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• 2019

Changes in the physical and chemical properties of soil materials during the nursing process have a great influence on the quality of containerized seedlings and on growth and survival after planting. In this study, the effect of biomaterials and their mixed ratios on the growth of Zelkova serrata seedlings in a containerized seedling production system was investigated. Mushroom sawdust, pine bark, and carbonized rice husk were used as biomaterials. The mixed ratios were 10% and 20% volume ratio of the growing medium volume, including the untreated controls. There was no significant difference in the height growth of the Zelkova serrata seedlings according to the biomaterials. The root collar diameter was the highest with the 20% carbonized rice husk and the lowest with the mushroom sawdust. The difference between the highest quality index and the lowest quality index was 30% in the order of the carbonized rice husk, pine bark, control, and mushroom sawdust, but there was no statistical significance. In this study, if the growing medium mixed with biomaterials does not reduce the seedling growth compared with the control, it is considered that the biomaterial can replace a part of the growing media. Therefore, the results show that some of the growing media can be replaced with carbonized rice husk or pine bark when producing Zelkova serrata seedlings.

• Journal of the Korean Wood Science and Technology
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• v.30 no.2
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• pp.128-133
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• 2002

This study was conducted to investigate the influence of storage time on the sapstain development of Japanese red pine and Korean pine logs during storage in log yard, and their stain characteristics. Japanese red pine and Korean pine trees were harvested and cut into logs in mid January of 2001. These logs were transported to the two local sawmills where they were closely stacked in remote parts of log yard. The logs were then sampled destructively by cutting seven to nine 3-cm long discs along the length of each log at intervals of 3, 4, 5, 6, and 8 month after felling. The stain coverage and maximal radial penetration of stain were measured from the discs of the sampled logs after the isolation of causal staining fungi. The sapstain was primarily infested by the attack of bark beetles and the species of bark beetle was identified as Tomicus piniperda. The main fungal species isolated from stained wood was Ophiostoma species. Based on the present study, the logs could be stored in log yard until May without stain; but stain development was rapid after May and the severity of stain increased proportionally with storage time. Korean pine was more susceptible to fungal stain than Japanese red pine. During summer storage, decay started to develop in logs and the main species were identified as Tyromyces sp. and Schizophyllum commune. Information provided in this paper would be very useful to develop more effective control strategies for sapstain prevention in Japanese red pine and Korean pine logs.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.33 no.4
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• pp.42-48
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• 2001

The spent liquor (BSL) obtained from alkaline sulfite-anthraquinone (AS-AQ) cooking of pine bark was applied as a deinking agent. In the ONP deinking system, although BSL removed the small size of ink particles (${\geq}$$12.5{\mu}m$), it did not improve the brightness of pulp. The brightness of pulp slightly improved when treated with BSL at 150~$180^{\circ}C$. However, the brightness of the pulp was lower than that of a commercial deinking agent (oleic acid). It seems that BSL treated with a high temperature still had a high dispersing ability on the ink particles. Intensive oxidations were introduced, and remarkable deinking effects were observed at 13kgf/$cm^2$ oxygen pressure, $160^{\circ}C$ and 2h. When BSL treated at 13kgf/$cm^2$ oxygen pressure, $170^{\circ}C$ and a time period of 30~90 min is added to the pulp, at 0.1% or lower, the deinking effect became higher than that of oleic acid. On the other hand, additional alkali treatments during the oxidation possibility as a deinking agent when oxidized. However, since the oxidized BSL still has a high dispersing ability and lower molecular weight, several modifications need to be done in the near future in order to be applied as a high quality deinking agent.

• Journal of the Korean Wood Science and Technology
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• v.22 no.2
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• pp.54-60
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• 1994

The pine bark from mechanical pulp industry was cooked with bisulfite liquor, and the chemical and physical properties of the spent liquor after cooking for 90 minutes, which allowed partial defibration were determined. Lignosulfonate or lignin-related materials in the spent liquor have a molecular weight of approximately 3,000, which is lower than that of commercial lignosulfonate (C-LS) prepared from wood. The sulfonic equivalent weight calculated by colloid titration was 307 for C-LS, 238~239 for spent liquor. The contents of lignosulfonic acid and phenolic hydroxyl groups on $C_9$ unit of lignin in the spent liquor were 0.98~0.99, and 1.51~1.66, respectively. These values were remarkably higher than those of C-LS. Especially the content of phenolic hydroxyl group was almost twice that of C-LS. The viscosity of spent liquor after freeze drying was very low compared to C-LS. The dispersing ability of spent liquor was almost equivalent to or more than that of C-LS.

• Journal of the Korean Wood Science and Technology
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• v.24 no.2
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• pp.81-93
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• 1996

To elucidate the structure of procyanidin in Korean red pine (Pinus densiflora S. et Z.), bark, the extractives were extracted with acetone-water mixture(7:3, v/v) from inner bark of Korean red pine. The extracts separated three fractions which were extracted by liquid-liquid extraction. The extracting solvents were chloroform and ethyl acetate and water. The part of ethylacetate soluble was chromatographed by liquid chromatography. The ethylacetate soluble portion yielded four natural procyanidin dimers, two known epicatechin-($4{\beta}{\rightarrow}6$)-catechin, catechin-($4{\alpha}{\rightarrow}8$)-catechin and two unknown catechin-($4{\beta}{\rightarrow}6$)-catechin and conformational isomer of epicatechin-($4{\alpha}{\rightarrow}6$)-catechin. The additional catechins was also isolated. The structures of these procyanidins were elucidated by their $^{13}C$-NMR spectra.

• Journal of Korean Society of Environmental Engineers
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• v.29 no.8
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• pp.930-937
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• 2007

Korean pine(Pinus densiflora) bark was evaluated for its adsorption capacity of Cu(II) ions from aqueous solution by running a series of batch experiments. Prior to the tests, the milled barks were treated with 1 N NaOH or 1 N HCl to examine the effect of surface modification. For comparison, untreated bark was tested under same condition. Within the tested pH range between 3 and 6, NaOH treatment increased Cu(II) adsorption capacity by $139\sim184%$, while HCl treatment decreased it by $37\sim42%$. Maximum copper ion uptake by bark was observed at pH $5\sim6$, but pH of solution was not a potent influence. A pseudo second-order kinetic model fitted well for the sorption of copper ion onto bark. For NaOH-treated bark, the calculated sorption capacity$(q_e)$ increased from 6.58 to 12.77 mg/g, while the equilibrium rate constant$(k_2)$ decreased from 0.284 to 0.014 g/mg/min as initial Cu(II) concentration doubled from 100 mg/L. A batch isotherm test using NaOH-treated bark showed that equilibrium sorption data were represented by both the Langmuir model and the Freundlich model. It was confirmed that carboxylic acid of bark was involved in the Cu(II) adsorption. For NaOH-treated bark, in particular, carboxylate ion produced by hydrolysis or saponification appeared to be a major functional roup responsible for the enhanced Cu(II) sorption.