• The Plant Pathology Journal
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• 제29권3호
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• pp.344-349
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• 2013

Intraspecific variation in morphometry of pine wood nematodes Bursaphelenchus xylophilus and B. mucronatus in relation to geographical locations in South Korea was investigated using morphometric characters (body length, a, b and c ratio, stylet length, and spicule length for a male nematode and V (%) value for a female nematode). B. xylophilus was isolated from Pinus thunbergii in Jinju (1998), Ulsan (2000), Yangsan (2000), Mokpo (2001) and Jeju (2004), and from P. densiflora in Gumi (2001). B. mucronatus was isolated from P. thunbergii in Jinju (1991) and from P. densiflora in Milyang (2001). The body length of male and female B. xylophilus had the highest coefficient of variability and showed significant differences among geographical locations. The V (%) value for female B. xylophilus showed the lowest coefficient of variability, changing little with geographical area and host plant. All morphometric characters in B. mucronatus except for stylet length and female body length showed no significant differences between locations or hosts, suggesting they may not be affected by geographical area or host plant.

• Molecules and Cells
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• 제20권2호
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• pp.201-209
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• 2005

We have constructed an AFLP-based linkage map of Japanese red pine (Pinus densiflora Siebold et Zucc.) using haploid DNA samples of 96 megagametophytes from a single maternal tree, selection clone Kyungbuk 4. Twenty-eight primer pairs generated a total of 5,780 AFLP fragments. Five hundreds and thirteen fragments were verified as genetic markers with two alleles by their Mendelian segregation. At the linkage criteria LOD 4.0 and maximum recombination fraction 0.25(${\theta}$), a total of 152 markers constituted 25 framework maps for 19 major linkage groups. The maps spanned a total length of 2,341 cM with an average framework marker spacing of 18.4 cM. The estimated genome size was 2,662 cM. With an assumption of equal marker density, 82.2% of the estimated genome would be within 10 cM of one of the 230 linked markers, and 68.1% would be within 10 cM of one of the 152 framework markers. We evaluated map completeness in terms of LOD value, marker density, genome length, and map coverage. The resulting map will provide crucial information for future genomic studies of the Japanese red pine, in particular for QTL mapping of economically important breeding target traits.

• Food Science and Biotechnology
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• 제15권4호
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• pp.582-588
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• 2006

The antioxidant and antimicrobial activity of the fermentation extract (PFE) and the 50 and 80% ethanol extracts (PE 50, PE 80) of Pinus densiflora pine needles were evaluated. Electron donating ability, superoxide dismutase (SOD) ability, and antimicrobial activity were observed in PFE; those abilities differed in PE 80 and PE 50, depending on the ethanol concentration used for the extraction. PFE had the highest electron donating ability with a value of 92.20%, while PE 80 and PE 50 had values of 74.66 and 53.47%, respectively. For SOD activity, PE 80 exhibited a slightly higher value of 31.11% compared to that of PFE and PE 50, which were 29.65 and 25.43%, respectively. PFE, PE 50, and PE 80 were all found to inhibit bacteria, and the effectiveness of this inhibition was strongly related to the type of extracts used. PFE showed good antimicrobial effects for all of the tested Gram-positive strains and for most of the tested Gram-negative strains. These results suggest that PFE has superior functionality compared to the ethanol extracts (PE 80, PE 50), in terms of antioxidant and antimicrobial activity. On the basis of these results, pine needle fermentation extracts can be used for industrial applications as a functional material.

• 한국환경생태학회지
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• 제23권1호
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• pp.56-65
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• 2009

한국에서 소나무는 문화적, 역사적, 정서적으로 중요한 위치에 있으며, 목재자원 생산이 가능한 수종이다. 소나무의 점유면적은 점차 줄어드는 반면, 참나무류를 비롯한 활엽수림이 증가하는 추세이므로 소나무림 유지를 위한 갱신과 시업법에 관한 연구가 요구된다. 본 연구는 중부지역을 대상으로 소나무에 대한 임분유형 및 구조분석을 통하여 생육환경과 천이과정을 파악함으로서, 안정적이며 지속적인 경영림으로서의 발달과 천연갱신 유도에 그 목적이 있다. 중부지역 소나무림은 당단풍나무군락, 상수리나무군락, 비목군락, 전형군락으로 분류되었으며, 지역에 따라 유형별 다른 특성을 보이고 있었다. 상층에는 소나무가 높게 나타나고 있으나, 중층에서는 참나무류 등 활엽 교목성 수종의 중요치가 높았으며, 하층에서 소나무 출현은 빈약하였다. 따라서 상관적으로 소나무가 생태적 영향력이 높은 것처럼 나타나고 있지만, 차후 자연 상태에서 교목층의 소나무가 고사하면 아교목층 이하 신갈나무, 굴참나무, 졸참나무 등 참나무류가 우점할 것으로 사료되었다. 소나무림의 형질과 생장패턴은 임분유형에 따라 지역별 차이를 보이고 있어 소나무림에 대한 시업도 차별 적용되어야 할 것으로 사료되었다.

• The Korean Journal of Ecology
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• 제20권5호
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• pp.315-321
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• 1997

Peroxidase activities and isozyme patte군 of the pine needles (Pinus densiflora) were examined and compared in the coastal regions of Anmyum-Do(Choongnam, Taean-Gun) and inland regions of Shinchang-Myun(Choongnam, Asan-City). The pine needle peroxidase from Anmyum-Do showed approximately three times higher specfic activity than Shinchang pine needle peroxidase. The pine needle extracts of Anmyun-Do and Shinchang contained three anionic isoperoxidases, named A1, A2 and A3, when subjected to starch gel electrophoresis at pH 7.0. Cjationic isoperoxidases could not be found in both extracts., However, there existed unique isoperoxidase An only from the extracts of Anmyun-Do pine needles under the salinary environment. Moreover, the specific activities of catalase and glucose-6-phosphate dehydrogenase from Anmyun-Do, known for the inducible enzymes under the stress condition, were about 1.8 times higher than those of Shinchang pine needles. However, the specific activities of other enzymes did not show great differences between the two regions. Considering the above results of the higher specific activity of peroxidase and the unique expression of isoperoxidase An, pine needle peroxidase might involve in the defence mechanism against the salinary stress of Anmyun-Do.

• The Korean Journal of Ecology
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• 제25권6호
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• pp.389-393
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• 2002

Effects of environmental factors on in vitro pine pollen performance were investigated. Pinus densiflora and P. rigida pollen grains collected at Mt. Kwanak, Korea were used. Three environmental factors, such as pollen storage temperature, pollen culture temperature and nutrient condition in medium, were tested. To determine the storage temperature effects on pollen viability, pine pollen was stored at $-70^{\circ}C$, $-12^{\circ}C$, $4^{\circ}C$ and $22^{\circ}C$. Pollen viability was substantially extended at the storage temperatures of $-12^{\circ}C$ and $4^{\circ}C$ for more than 300 days. To elucidate the culture temperature effects on pine pollen germination and tube growth, pollen grains were cultured at the temperatures from $5^{\circ}C$ to $40^{\circ}C$ at $5^{\circ}C$intervals. The germination rate and tube growth were highest at $25^{\circ}C$ and decreased above $30^{\circ}C$. To investigate boron and sucrose effects on pollen tube growth, the pollen was cultured at different sucrose and boric acid concentrations. Germination rate was optimal in germination medium containing 3 or 5$\%$ sucrose with 0.01 $\%$ boric acid. These results indicate that the pine pollen can be stored for considerable length of time without noticeable loss of viability at storage temperature below or near $0^{\circ}C$. Optimal germination medium conditions were established for pine pollen. Therefore, pine pollen can be used for many biological and environmental monitoring researches.

• 한국식품영양과학회지
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• 제27권4호
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• pp.568-573
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• 1998

Volatile compounds in Pinus densiflora were extracted with Likens-Nickerson apparatus for three hours, and were separated and identified by gas chromatography(GC) and mass selective detector(MSD). Twenty six compounds were isolated from Pinus densiflora, identified by GC-MSD and twelve compounds were confirmed by matching retention times of the pure comounds. The main valatile compounds were terpenoids such as limonene(36.2%), $\beta$-pinene(16.9%), $\beta$-myrcene(12.6%) and $\alpha$-pinene(10.9%), and the total amount of these main compounds was 367.9$\mu\textrm{g}$ per 1g of pine twigs.

• The Korean Journal of Ecology
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• 제24권6호
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• pp.341-348
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• 2001

Population dynamics of the oldest relic stand of Pinus densiflora for. erecta which remains as a status of patch in Sokwang-Ri area, Uljin-Gun, was studied to provide the basic data for conservation of the oldest pine stand in Korea. The upper slope site and ridge site including total 12 plots mainly represented Pinus densiflora for. erecta and Quercus mongolica. These two tree species showed reverse J-shaped distribution. From this information, it was concluded that intensive interspecific competition between two species could be occurring. Age distribution of P. densiflora for. erecta on the sites above middle slope area was indicated bi-modal type or tri-modal type which was known as an age structure of climax forest. Therefore, it was estimated that Pinus densiflora for. erecta population could be continuously maintained in the each patch above middle slope area. In the valley sites, hardwood species such as Q. mongolica, Q. variabilis, Styrax obassia and Acer pseudosieboldianum occupied most part of the under crown-cover area and their seedlings and saplings were developing into the closed upper layer crown because the height of upper layer crown reached about 35 meters and such a high crown height was more suitable for light utilization by plants of under layer. The growth patterns were mainly fluctuated for the entire life time of the oldest tree caused by frequent disturbance. And the initial growth curves of the mature trees and suppressed juvenile trees showed different tendencies along the sites.

• Journal of the Korean Wood Science and Technology
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• 제27권4호
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• pp.15-19
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• 1999

Three wood coffins of Chosun Dynasty period buried about 450 years ago were excavated in the sound condition in Andong area in the early 1998. The proprietors of wood coffins were grandparents, Mr. Myoung Jong Lee and Mrs. Mun, and their grandson, Mr. Eung Tae Lee, and the social standing of their family was known to belong to the nobility in those days by the clan genealogy. All the wood coffins investigated through light microscopy had same anatomical characteristics as follows: abrupt to somewhat abrupt tracheid transition from earlywood to latewood; normal longitudinal and horizontal resin canals with thin-walled epithelium; tylosoids in resin canals; bordered pits frequently in 1 row on radial walls of tracheids; 1 or 2 window-like pits per cross-field; uniseriate and fusiform rays; heterogeneous rays composed of body ray parenchyma cells and marginal ray tracheids or homgeneous rays composed of only ray tracheids; dentate ray tracheids; occasional trabeculae traversing tracheids in radial direction. Based on theses microscopic characteristics, all the wood coffins were identified to be Korean red pine (Pinus densiflora) or Korean black pine (Pinus thunbergii). Korean black pine growing naturally in coastal area might not be probable because the site of excavation, Andong area, was mountainous and inland area of Korea Thus, Korean red pine was thought to be the possible species for the wood coffins because of its natural distribution through the Korean Peninsula and the easy availability.

• 한국농림기상학회지
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• 제22권2호
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• pp.47-56
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• 2020

본 연구는 기후변화에 따른 소나무 잠재분포변화 예측 및 환경요인과의 관계를 파악하기 위한 목적으로 수행되었다. 입력자료인 종속변수는 1:5,000 임상도에서 추출한 소나무 출현자료를 사용하였으며, 독립변수는 RCP 시나리오 기후자료 및 임상도, 입지도에서 추출한 기후, 입지, 생육환경자료 등 총 14개의 환경요인 변수를 사용하였다. 이러한 입력자료를 바탕으로 생태적 지위 개념을 기반으로 한 종 분포 모형 중 하나인 MaxEnt (Maximum Entropy Modeling) 모형을 구동하여 미래의 소나무 잠재분포를 예측하였다. 분석결과 training AUC (Area Under Curve)가 0.79로 우수한 수준의 정확도를 보였으며 현존 소나무 분포 자료와 유사한 현재 잠재분포 결과를 보였다. RCP 시나리오를 적용한 결과 소나무 잠재분포지는 시간이 지남에 따라 지속적으로 감소할 것으로 나타났으며 RCP8.5 기준으로 2050년과 2070년에 각각 11.1%, 18.7%의 잠재분포지가 줄어들 것으로 예측되었다. 입력자료의 소나무 잠재분포 판단에 대한 기여도는 계절기온, 고도, 겨울철 기온 등이 높게 나타났다. 본 연구의 결과는 기후변화로 인한 소나무림 보전 및 대책 수립을 위한 기초자료로 활용될 것으로 판단된다.